################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BseRI BsgI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 183 GTAC GT^AC AflIII 311 ACATGT A^C[GA][CT]GT AluI 489 AGCT AG^CT AseI 369 ATTAAT AT^TAAT AsnI 369 ATTAAT AT^TAAT AspHI 31 GTGCTC G[AT]GC[AT]^C AvaII 471 GGACC G^G[AT]CC BbsI 460 GAAGAC GAAGAC(2/6) BglII 11 AGATCT A^GATCT BpuAI 460 GAAGAC GAAGAC(2/6) BsaBI 217 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 67 CCTGGG C^C[ACGT][ACGT]GG BsaWI 54 TCCGGT [AT]^CCGG[AT] BsiEI 74 CGGCCG CG[GA][CT]^CG BsiHKAI 31 GTGCTC G[AT]GC[AT]^C BsmAI 292 GAGAC GTCTC(1/5) BspLU11I 311 ACATGT A^CATGT BsrBI 44 CCGCTC CCGCTC(-3/-3) BstF5I 465 CATCC GGATG(2/0) BstYI 11 AGATCT [GA]^GATC[CT] Cfr13I 471 GGACC G^G[ACGT]CC EagI 74 CGGCCG C^GGCCG EclXI 74 CGGCCG C^GGCCG Eco52I 74 CGGCCG C^GGCCG FauI 121 CCCGC CCCGC(4/6) FokI 465 CATCC GGATG(9/13) HinfI 521 GAATC G^A[ACGT]TC MamI 217 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 11 AGATCT [GA]^GATC[CT] MseI 370 TTAA T^TAA MwoI 51 GCCTCCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 471 GGACCC GG[ACGT]^[ACGT]CC NspI 311 ACATGT [GA]CATG^[CT] PshBI 369 ATTAAT AT^TAAT RsaI 183 GTAC GT^AC Sau96I 471 GGACC G^G[ACGT]CC SfcI 287 CTGTAG C^T[GA][CT]AG StuI 164 AGGCCT AGG^CCT TfiI 521 GAATC G^A[AT]TC Tru9I 370 TTAA T^TAA Tsp509I 368 AATT ^AATT TspRI 285 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 186 CCAAGTTTTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 11 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BmyI 31 GTGCTC G[AGT]GC[ACT]^C 560 GGGCAC BpmI 233 CTCCAG CTGGAG(16/14) 172 CTGGAG Bsp1286I 31 GTGCTC G[AGT]GC[ACT]^C 560 GGGCAC BsrFI 76 GCCGGC [GA]^CCGG[CT] 124 GCCGGC BsrI 430 ACTGG ACTGG(1/-1) 456 ACTGG Cac8I 76 GCCGGC GC[ACGT]^[ACGT]GC 124 GCCGGC Cfr10I 76 GCCGGC [GA]^CCGG[CT] 124 GCCGGC DpnII 12 GATC ^GATC 177 GATC MboI 12 GATC ^GATC 177 GATC NaeI 76 GCCGGC GCC^GGC 124 GCCGGC NdeII 12 GATC ^GATC 177 GATC NgoMIV 76 GCCGGC G^CCGGC 124 GCCGGC NlaIII 153 CATG CATG^ 312 CATG Sau3AI 12 GATC ^GATC 177 GATC SfaNI 513 GCATC GCATC(5/9) 534 GCATC TaqI 95 TCGA T^CGA 179 TCGA TthHB8I 95 TCGA T^CGA 179 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI BbvI BssKI BstNI DdeI EaeI EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MboII MnlI MspI MvaI ScrFI TseI