(Circular) (Six-base) MAPSORT of: pFN21AE2594.seq Check: 9840 from: 1 to: 15697 pFN21AE2594 15697 bp With 182 enzymes: SgfI * January 6, 2016 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5270 8747 5270 Size: 3477 12220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4204 13507 278 Size: 53 83 186 936 2668 9303 2468 Fragments arranged by size: 9303 2668 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7104 9539 13040 1937 Size: 5167 2435 3501 4594 Fragments arranged by size: 5167 4594 3501 2435 Acc65I G'GTAC_C Cuts at: 13018 13018 Size: 15697 AclI AA'CG_TT Cuts at: 1563 12843 13824 14197 1563 Size: 11280 981 373 3063 Fragments arranged by size: 11280 3063 981 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2397 3087 3108 4494 4739 5490 6024 Size: 522 243 690 21 1386 245 751 534 Cuts at: 6024 7431 7802 10458 10907 12063 12530 12648 13760 Size: 1407 371 2656 449 1156 467 118 1112 Cuts at: 13760 15144 15412 1632 Size: 1384 268 1917 Fragments arranged by size: 2656 1917 1407 1386 1384 1156 1112 751 690 534 522 467 449 371 268 245 243 118 21 AfeI AGC'GCT Cuts at: 3383 4355 11352 3383 Size: 972 6997 7728 Fragments arranged by size: 7728 6997 972 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 15500 AflIII A'CryG_T Cuts at: 1203 5067 8030 8460 11608 14596 15573 1203 Size: 3864 2963 430 3148 2988 977 1327 Fragments arranged by size: 3864 3148 2988 2963 1327 977 430 AgeI A'CCGG_T Cuts at: 9319 11922 14528 9319 Size: 2603 2606 10488 Fragments arranged by size: 10488 2606 2603 AhdI GACnn_n'nnGTC Cuts at: 14426 14426 Size: 15697 AleI CACnn'nnGTG Cuts at: 1166 4688 8051 10133 1166 Size: 3522 3363 2082 6730 Fragments arranged by size: 6730 3522 3363 2082 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 11210 12124 12173 1184 Size: 10026 914 49 4708 Fragments arranged by size: 10026 4708 914 49 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4381 4413 5767 5799 4381 Size: 32 1354 32 14279 Fragments arranged by size: 14279 1354 32 32 AlwNI CAG_nnn'CTG Cuts at: 2776 2951 6869 7262 7352 7409 7439 8770 9686 Size: 175 3918 393 90 57 30 1331 916 Cuts at: 9686 11519 11845 12538 12566 13440 15012 2776 Size: 1833 326 693 28 874 1572 3461 Fragments arranged by size: 3918 3461 1833 1572 1331 916 874 693 393 326 175 90 57 30 28 ApaI G_GGCC'C Cuts at: 8060 10071 11019 11404 12035 12639 8060 Size: 2011 948 385 631 604 11118 Fragments arranged by size: 11118 2011 948 631 604 385 ApaLI G'TGCA_C Cuts at: 2178 8760 11891 13754 14910 2178 Size: 6582 3131 1863 1156 2965 Fragments arranged by size: 6582 3131 2965 1863 1156 ApoI r'AATT_y Cuts at: 1493 2112 3077 3086 3266 3296 5133 5927 5998 Size: 619 965 9 180 30 1837 794 71 Cuts at: 5998 6293 6421 9232 9901 10541 12434 13006 13232 Size: 295 128 2811 669 640 1893 572 226 Cuts at: 13232 13384 15493 1493 Size: 152 2109 1697 Fragments arranged by size: 2811 2109 1893 1837 1697 965 794 669 640 619 572 295 226 180 152 128 71 30 9 AseI AT'TA_AT Cuts at: 160 1032 14251 160 Size: 872 13219 1606 Fragments arranged by size: 13219 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 15697 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2257 2268 2523 2679 3960 4035 Size: 73 102 212 11 255 156 1281 75 Cuts at: 4035 4647 7217 7578 10739 11011 11096 11364 11471 Size: 612 2570 361 3161 272 85 268 107 Cuts at: 11471 11505 13022 1870 Size: 34 1517 4545 Fragments arranged by size: 4545 3161 2570 1517 1281 612 361 272 268 255 212 156 107 102 85 75 73 34 11 AvrII C'CTAG_G Cuts at: 2612 5840 2612 Size: 3228 12469 BamHI G'GATC_C Cuts at: 5824 9468 13027 13394 5824 Size: 3644 3559 367 8127 Fragments arranged by size: 8127 3644 3559 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 6136 6997 7704 8997 Size: 325 217 627 462 3887 861 707 1293 Cuts at: 8997 11659 13018 14473 618 Size: 2662 1359 1455 1842 Fragments arranged by size: 3887 2662 1842 1455 1359 1293 861 707 627 462 325 217 BanII G_rGCy'C Cuts at: 1391 2550 2620 2997 6774 7773 8060 8084 9485 Size: 1159 70 377 3777 999 287 24 1401 Cuts at: 9485 9535 10028 10071 10409 10746 11019 11371 11404 Size: 50 493 43 338 337 273 352 33 Cuts at: 11404 12035 12054 12639 13016 1391 Size: 631 19 585 377 4072 Fragments arranged by size: 4072 3777 1401 1159 999 631 585 493 377 377 352 338 337 287 273 70 50 43 33 24 19 BbeI G_GCGC'C Cuts at: 7001 7708 9001 7001 Size: 707 1293 13697 Fragments arranged by size: 13697 1293 707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4500 5391 5402 6246 6977 7025 8463 Size: 971 2601 891 11 844 731 48 1438 Cuts at: 8463 10478 928 Size: 2015 6147 Fragments arranged by size: 6147 2601 2015 1438 971 891 844 731 48 11 BbvCI CC'TCA_GC Cuts at: 5093 8103 8550 8878 11682 12066 5093 Size: 3010 447 328 2804 384 8724 Fragments arranged by size: 8724 3010 2804 447 384 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3597 3631 5473 5507 13888 13922 1903 Size: 34 1660 34 1842 34 8381 34 3678 Fragments arranged by size: 8381 3678 1842 1660 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 11819 13589 14799 11819 Size: 1770 1210 12717 Fragments arranged by size: 12717 1770 1210 BclI T'GATC_A Cuts at: 1548 5365 1548 Size: 3817 11880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8576 11675 12824 Size: 107 122 71 631 605 6904 3099 1149 Cuts at: 12824 14308 136 Size: 1484 1525 Fragments arranged by size: 6904 3099 1525 1484 1149 631 605 122 107 71 BglII A'GATC_T Cuts at: 6118 8240 8740 12885 15692 6118 Size: 2122 500 4145 2807 6123 Fragments arranged by size: 6123 4145 2807 2122 500 BlpI GC'TnA_GC Cuts at: 7353 13114 7353 Size: 5761 9936 Bme1580I G_kGCm'C Cuts at: 1790 2182 8060 8764 9288 10071 10154 11019 11199 Size: 392 5878 704 524 783 83 865 180 Cuts at: 11199 11404 11895 12035 12271 12639 13758 14914 1790 Size: 205 491 140 236 368 1119 1156 2573 Fragments arranged by size: 5878 2573 1156 1119 865 783 704 524 491 392 368 236 205 180 140 83 BmgBI CAC'GTC Cuts at: 1318 5068 8033 8461 1318 Size: 3750 2965 428 8554 Fragments arranged by size: 8554 3750 2965 428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2634 2743 2959 Size: 416 53 83 399 24 1217 109 216 Cuts at: 2959 5276 5784 8404 8488 11186 11194 11316 11820 Size: 2317 508 2620 84 2698 8 122 504 Cuts at: 11820 13428 14386 442 Size: 1608 958 1753 Fragments arranged by size: 2698 2620 2317 1753 1608 1217 958 508 504 416 399 216 122 109 84 83 53 24 8 BmtI G_CTAG'C Cuts at: 1056 2784 3468 1056 Size: 1728 684 13285 Fragments arranged by size: 13285 1728 684 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2283 2283 Size: 15697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2824 2904 3903 4236 4958 7416 8236 8537 Size: 1585 80 999 333 722 2458 820 301 Cuts at: 8537 8745 8841 9534 9580 9753 9822 10116 11194 Size: 208 96 693 46 173 69 294 1078 Cuts at: 11194 11822 11881 12689 14357 1239 Size: 628 59 808 1668 2579 Fragments arranged by size: 2579 2458 1668 1585 1078 999 820 808 722 693 628 333 301 294 208 173 96 80 69 59 46 Bpu10I CC'TnA_GC Cuts at: 4902 5093 8103 8550 8878 9209 10408 11273 11682 Size: 191 3010 447 328 331 1199 865 409 Cuts at: 11682 12027 12066 12243 4902 Size: 345 39 177 8356 Fragments arranged by size: 8356 3010 1199 865 447 409 345 331 328 191 177 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3506 4880 5221 5634 7544 7913 9075 9321 10602 Size: 1374 341 413 1910 369 1162 246 1281 Cuts at: 10602 12147 12533 12773 13822 14687 14985 3506 Size: 1545 386 240 1049 865 298 4218 Fragments arranged by size: 4218 1910 1545 1374 1281 1162 1049 865 413 386 369 341 298 246 240 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2214 3710 4143 4765 5868 10904 12478 Size: 617 715 1496 433 622 1103 5036 1574 Cuts at: 12478 14360 882 Size: 1882 2219 Fragments arranged by size: 5036 2219 1882 1574 1496 1103 715 622 617 433 BsaAI yAC'GTr Cuts at: 493 1204 6251 12877 13488 493 Size: 711 5047 6626 611 2702 Fragments arranged by size: 6626 5047 2702 711 611 BsaBI GATnn'nnATC Cuts at: 5823 15691 5823 Size: 9868 5829 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4201 6998 7338 7705 Size: 53 83 186 936 2668 2797 340 367 Cuts at: 7705 8998 13504 13886 14521 275 Size: 1293 4506 382 635 1451 Fragments arranged by size: 4506 2797 2668 1451 1293 936 635 382 367 340 186 83 53 BsaWI w'CCGG_w Cuts at: 4287 4399 9319 11922 14130 14528 14802 14949 15360 Size: 112 4920 2603 2208 398 274 147 411 Cuts at: 15360 4287 Size: 4624 Fragments arranged by size: 4920 4624 2603 2208 411 398 274 147 112 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2557 2587 3381 3411 9532 Size: 30 165 30 1154 30 794 30 6121 Cuts at: 9532 9562 9578 9608 9751 9781 10773 10803 12842 Size: 30 16 30 143 30 992 30 2039 Cuts at: 12842 12872 1178 Size: 30 4003 Fragments arranged by size: 6121 4003 2039 1154 992 794 165 143 30 30 30 30 30 30 30 30 30 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2154 2343 2757 4174 4276 5661 7566 7907 Size: 968 189 414 1417 102 1385 1905 341 Cuts at: 7907 7910 8120 8123 9174 9177 9344 9536 9924 Size: 3 210 3 1051 3 167 192 388 Cuts at: 9924 11070 11352 11489 12027 12054 1186 Size: 1146 282 137 538 27 4829 Fragments arranged by size: 4829 1905 1417 1385 1146 1051 968 538 414 388 341 282 210 192 189 167 137 102 27 3 3 3 BseYI C'CCAG_C Cuts at: 3526 5243 7272 7402 8704 9309 9389 10064 10976 Size: 1717 2029 130 1302 605 80 675 912 Cuts at: 10976 11018 11026 11129 11382 11434 11460 11958 12093 Size: 42 8 103 253 52 26 498 135 Cuts at: 12093 12120 12217 12398 14900 15396 3526 Size: 27 97 181 2502 496 3827 Fragments arranged by size: 3827 2502 2029 1717 1302 912 675 605 498 496 253 181 135 130 103 97 80 52 42 27 26 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2937 3128 5701 8607 10215 10236 11433 Size: 573 1201 191 2573 2906 1608 21 1197 Cuts at: 11433 11543 11546 11903 12084 12095 12590 1163 Size: 110 3 357 181 11 495 4270 Fragments arranged by size: 4270 2906 2573 1608 1201 1197 573 495 357 191 181 110 21 11 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7952 10390 13147 13908 14936 1540 Size: 275 186 5951 2438 2757 761 1028 2301 Fragments arranged by size: 5951 2757 2438 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2182 6774 8084 8764 10058 10452 11895 13758 13843 Size: 4592 1310 680 1294 394 1443 1863 85 Cuts at: 13843 14914 2182 Size: 1071 2965 Fragments arranged by size: 4592 2965 1863 1443 1310 1294 1071 680 394 85 BsmI GAATG_Cn' Cuts at: 9355 10006 10165 10207 13208 13301 9355 Size: 651 159 42 3001 93 11751 Fragments arranged by size: 11751 3001 651 159 93 42 BsmBI CGTCTCn'nnnn_ Cuts at: 4227 4414 7045 9988 12589 4227 Size: 187 2631 2943 2601 7335 Fragments arranged by size: 7335 2943 2631 2601 187 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2182 2550 2620 2997 6774 7773 8060 Size: 399 392 368 70 377 3777 999 287 Cuts at: 8060 8084 8764 9288 9485 9535 10028 10058 10071 Size: 24 680 524 197 50 493 30 13 Cuts at: 10071 10154 10409 10452 10746 11019 11199 11371 11404 Size: 83 255 43 294 273 180 172 33 Cuts at: 11404 11895 12035 12054 12271 12639 13016 13758 13843 Size: 491 140 19 217 368 377 742 85 Cuts at: 13843 14914 1391 Size: 1071 2174 Fragments arranged by size: 3777 2174 1071 999 742 680 524 493 491 399 392 377 377 368 368 294 287 273 255 217 197 180 172 140 85 83 70 50 43 33 30 24 19 13 BspEI T'CCGG_A Cuts at: 4399 15360 4399 Size: 10961 4736 BspHI T'CATG_A Cuts at: 2593 12975 13586 15316 2593 Size: 10382 611 1730 2974 Fragments arranged by size: 10382 2974 1730 611 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5270 5344 7078 7116 7924 8747 12235 12819 Size: 4426 74 1734 38 808 823 3488 584 Cuts at: 12819 13052 844 Size: 233 3489 Fragments arranged by size: 4426 3489 3488 1734 823 808 584 233 74 38 BsrBI CCG'CTC Cuts at: 1394 1823 2395 2530 2707 7755 13584 1394 Size: 429 572 135 177 5048 5829 3507 Fragments arranged by size: 5829 5048 3507 572 429 177 135 BsrDI GCAATG_nn' Cuts at: 65 1248 9675 9843 10536 12984 14192 14366 65 Size: 1183 8427 168 693 2448 1208 174 1396 Fragments arranged by size: 8427 2448 1396 1208 1183 693 174 168 BsrFI r'CCGG_y Cuts at: 1700 1810 7875 8203 8882 9319 10927 11922 14341 Size: 110 6065 328 679 437 1608 995 2419 Cuts at: 14341 14528 1700 Size: 187 2869 Fragments arranged by size: 6065 2869 2419 1608 995 679 437 328 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 15697 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 15697 BssSI C'ACGA_G Cuts at: 2376 5630 6375 13757 14769 2376 Size: 3254 745 7382 1012 3304 Fragments arranged by size: 7382 3304 3254 1012 745 BstAPI GCAn_nnn'nTGC Cuts at: 794 2951 7058 10202 10304 10634 11890 12538 794 Size: 2157 4107 3144 102 330 1256 648 3953 Fragments arranged by size: 4107 3953 3144 2157 1256 648 330 102 BstBI TT'CG_AA Cuts at: 3581 3913 6100 6427 14501 3581 Size: 332 2187 327 8074 4777 Fragments arranged by size: 8074 4777 2187 332 327 BstEII G'GTnAC_C Cuts at: 1184 2733 7178 7739 9379 11869 1184 Size: 1549 4445 561 1640 2490 5012 Fragments arranged by size: 5012 4445 2490 1640 1549 561 BstXI CCAn_nnnn'nTGG Cuts at: 3386 8053 8509 10531 3386 Size: 4667 456 2022 8552 Fragments arranged by size: 8552 4667 2022 456 BstYI r'GATC_y Cuts at: 1972 2129 2518 2834 4933 5824 6118 6681 7252 Size: 157 389 316 2099 891 294 563 571 Cuts at: 7252 8240 8740 9468 10512 12885 13027 13394 13779 Size: 988 500 728 1044 2373 142 367 385 Cuts at: 13779 13796 15248 15334 15346 15692 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2373 2099 1977 1452 1044 988 891 728 571 563 500 389 385 367 346 316 294 157 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3933 7938 8037 9605 3933 Size: 4005 99 1568 10025 Fragments arranged by size: 10025 4005 1568 99 BtgI C'CryG_G Cuts at: 513 1065 2004 7734 8085 8526 9527 10143 11579 Size: 552 939 5730 351 441 1001 616 1436 Cuts at: 11579 11640 11875 12489 12993 513 Size: 61 235 614 504 3217 Fragments arranged by size: 5730 3217 1436 1001 939 616 614 552 504 441 351 235 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 8521 10578 487 Size: 683 56 7295 2057 5606 Fragments arranged by size: 7295 5606 2057 683 56 BtsI GCAGTG_nn' Cuts at: 2633 2884 2893 3793 7341 7506 8268 8926 9416 Size: 251 9 900 3548 165 762 658 490 Cuts at: 9416 10203 10224 10305 13213 14007 14027 2633 Size: 787 21 81 2908 794 20 4303 Fragments arranged by size: 4303 3548 2908 900 794 787 762 658 490 251 165 81 21 20 9 ClaI AT'CG_AT Cuts at: 1554 5493 1554 Size: 3939 11758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 9658 9693 529 Size: 35 9094 35 6533 Fragments arranged by size: 9094 6533 35 35 DraI TTT'AAA Cuts at: 4983 5925 13001 4983 Size: 942 7076 7679 Fragments arranged by size: 7679 7076 942 DraIII CAC_nnn'GTG Cuts at: 8938 11411 8938 Size: 2473 13224 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 12335 14704 809 Size: 507 11019 2369 1802 Fragments arranged by size: 11019 2369 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 5973 8655 Size: 54 1425 50 275 18 262 3881 2682 Cuts at: 8655 10266 10521 10644 10929 11342 12136 12748 13144 Size: 1611 255 123 285 413 794 612 396 Cuts at: 13144 14033 15662 8 Size: 889 1629 43 Fragments arranged by size: 3881 2682 1629 1611 1425 889 794 612 413 396 285 275 262 255 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 13144 1537 Size: 275 11332 4090 Fragments arranged by size: 11332 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 3123 3847 5910 6373 6552 7008 7526 Size: 702 1068 724 2063 463 179 456 518 Cuts at: 7526 7884 9561 12348 13627 1353 Size: 358 1677 2787 1279 3423 Fragments arranged by size: 3423 2787 2063 1677 1279 1068 724 702 518 463 456 358 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 14284 14658 14804 387 Size: 1064 138 12695 374 146 1280 Fragments arranged by size: 12695 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 6772 8082 6772 Size: 1310 14387 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2397 2824 2904 3087 3108 3903 Size: 393 522 243 427 80 183 21 795 Cuts at: 3903 4236 4494 4739 4958 5490 6024 7416 7431 Size: 333 258 245 219 532 534 1392 15 Cuts at: 7431 7802 8236 8537 8745 8841 9534 9580 9753 Size: 371 434 301 208 96 693 46 173 Cuts at: 9753 9822 10116 10458 10907 11194 11822 11881 12063 Size: 69 294 342 449 287 628 59 182 Cuts at: 12063 12530 12648 12689 13760 14357 15144 15412 1239 Size: 467 118 41 1071 597 787 268 1524 Fragments arranged by size: 1524 1392 1071 795 787 693 628 597 534 532 522 467 449 434 427 393 371 342 333 301 294 287 268 258 245 243 219 208 183 182 173 118 96 80 69 59 46 41 21 15 EcoNI CCTnn'n_nnAGG Cuts at: 9710 11285 12042 9710 Size: 1575 757 13365 Fragments arranged by size: 13365 1575 757 EcoO109I rG'GnC_Cy Cuts at: 2342 2805 7221 7959 8057 8593 9715 10013 10088 Size: 463 4416 738 98 536 1122 298 75 Cuts at: 10088 10613 10918 11015 11400 11401 11677 12031 12032 Size: 525 305 97 385 1 276 354 1 Cuts at: 12032 12186 12535 12635 12636 2342 Size: 154 349 100 1 5403 Fragments arranged by size: 5403 4416 1122 738 536 525 463 385 354 349 305 298 276 154 100 98 97 75 1 1 1 EcoRI G'AATT_C Cuts at: 5998 9232 12434 13006 13384 5998 Size: 3234 3202 572 378 8311 Fragments arranged by size: 8311 3234 3202 572 378 EcoRV GAT'ATC Cuts at: 2659 5713 2659 Size: 3054 12643 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3083 3218 4727 4892 5153 6362 7656 8993 9669 Size: 135 1509 165 261 1209 1294 1337 676 Cuts at: 9669 9934 12814 3083 Size: 265 2880 5966 Fragments arranged by size: 5966 2880 1509 1337 1294 1209 676 265 261 165 135 FspI TGC'GCA Cuts at: 14203 14203 Size: 15697 HaeII r_GCGC'y Cuts at: 1678 3385 4357 4966 7001 7708 8771 9001 10500 Size: 1707 972 609 2035 707 1063 230 1499 Cuts at: 10500 11153 11354 14844 1678 Size: 653 201 3490 2531 Fragments arranged by size: 3490 2531 2035 1707 1499 1063 972 707 653 609 230 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2557 2589 3124 3156 5057 Size: 32 135 32 985 32 535 32 1901 Cuts at: 5057 5089 5607 5639 6392 6424 8327 8359 8387 Size: 32 518 32 753 32 1903 32 28 Cuts at: 8387 8419 8795 8827 9221 9253 9578 9610 10372 Size: 32 376 32 394 32 325 32 762 Cuts at: 10372 10391 10404 10423 10709 10741 12010 12042 14338 Size: 19 13 19 286 32 1269 32 2296 Cuts at: 14338 14370 14412 14444 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2296 1903 1901 1269 985 762 753 535 518 394 376 325 286 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2203 6454 9586 9733 10314 10999 Size: 988 281 265 4251 3132 147 581 685 Cuts at: 10999 13041 13287 669 Size: 2042 246 3079 Fragments arranged by size: 4251 3132 3079 2042 988 685 581 281 265 246 147 HindIII A'AGCT_T Cuts at: 748 4709 6339 7595 10880 748 Size: 3961 1630 1256 3285 5565 Fragments arranged by size: 5565 3961 3285 1630 1256 HpaI GTT'AAC Cuts at: 13287 13287 Size: 15697 KasI G'GCGC_C Cuts at: 6997 7704 8997 6997 Size: 707 1293 13697 Fragments arranged by size: 13697 1293 707 KpnI G_GTAC'C Cuts at: 13022 13022 Size: 15697 MfeI C'AATT_G Cuts at: 13296 13296 Size: 15697 MluI A'CGCG_T Cuts at: 15573 15573 Size: 15697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5821 7235 9105 9293 12981 14811 14995 1305 Size: 4516 1414 1870 188 3688 1830 184 2007 Fragments arranged by size: 4516 3688 2007 1870 1830 1414 188 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5975 10646 10 Size: 54 1425 343 4143 4671 5061 Fragments arranged by size: 5061 4671 4143 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4688 5817 6779 8051 10133 10376 11171 Size: 648 3522 1129 962 1272 2082 243 795 Cuts at: 11171 11192 11607 13655 14014 14173 518 Size: 21 415 2048 359 159 2042 Fragments arranged by size: 3522 2082 2048 2042 1272 1129 962 795 648 415 359 243 159 21 MspA1I CmG'CkG Cuts at: 1606 2820 3707 3781 5350 7093 7496 7832 7976 Size: 1214 887 74 1569 1743 403 336 144 Cuts at: 7976 10187 10232 10489 10843 11057 11374 11438 11642 Size: 2211 45 257 354 214 317 64 204 Cuts at: 11642 11901 12023 12221 12563 12682 13113 13790 14938 Size: 259 122 198 342 119 431 677 1148 Cuts at: 14938 15183 1606 Size: 245 2120 Fragments arranged by size: 2211 2120 1743 1569 1214 1148 887 677 431 403 354 342 336 317 259 257 245 214 204 198 144 122 119 74 64 45 NaeI GCC'GGC Cuts at: 7877 8884 10929 7877 Size: 1007 2045 12645 Fragments arranged by size: 12645 2045 1007 NarI GG'CG_CC Cuts at: 6998 7705 8998 6998 Size: 707 1293 13697 Fragments arranged by size: 13697 1293 707 NcoI C'CATG_G Cuts at: 513 1065 2004 7734 8085 11875 513 Size: 552 939 5730 351 3790 4335 Fragments arranged by size: 5730 4335 3790 939 552 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 15697 NgoMIV G'CCGG_C Cuts at: 7875 8882 10927 7875 Size: 1007 2045 12645 Fragments arranged by size: 12645 2045 1007 NheI G'CTAG_C Cuts at: 1052 2780 3464 1052 Size: 1728 684 13285 Fragments arranged by size: 13285 1728 684 NotI GC'GGCC_GC Cuts at: 13144 13144 Size: 15697 NruI TCG'CGA Cuts at: 2622 2622 Size: 15697 NspI r_CATG'y Cuts at: 1132 1229 3217 3547 11612 13055 14600 1132 Size: 97 1988 330 8065 1443 1545 2229 Fragments arranged by size: 8065 2229 1988 1545 1443 330 97 PasI CC'CwG_GG Cuts at: 1348 8310 10364 10777 10804 11287 11634 1348 Size: 6962 2054 413 27 483 347 5411 Fragments arranged by size: 6962 5411 2054 483 413 347 27 PciI A'CATG_T Cuts at: 11608 14596 11608 Size: 2988 12709 PflMI CCAn_nnn'nTGG Cuts at: 1108 12528 1108 Size: 11420 4277 PfoI T'CCnGG_A Cuts at: 2010 4213 8722 8820 12008 2010 Size: 2203 4509 98 3188 5699 Fragments arranged by size: 5699 4509 3188 2203 98 PmeI GTTT'AAAC Cuts at: 13001 13001 Size: 15697 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 13766 13798 15279 15311 13766 Size: 32 1481 32 14152 Fragments arranged by size: 14152 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2805 7959 10918 12186 2805 Size: 5154 2959 1268 6316 Fragments arranged by size: 6316 5154 2959 1268 PshAI GACnn'nnGTC Cuts at: 1613 10389 10916 11618 1613 Size: 8776 527 702 5692 Fragments arranged by size: 8776 5692 702 527 PsiI TTA'TAA Cuts at: 2238 13267 2238 Size: 11029 4668 PspOMI G'GGCC_C Cuts at: 8056 10067 11015 11400 12031 12635 8056 Size: 2011 948 385 631 604 11118 Fragments arranged by size: 11118 2011 948 631 604 385 PspXI vC'TCGA_Gb Cuts at: 3960 4647 11505 3960 Size: 687 6858 8152 Fragments arranged by size: 8152 6858 687 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3411 3443 6281 6313 3411 Size: 32 2838 32 12795 Fragments arranged by size: 12795 2838 32 32 PstI C_TGCA'G Cuts at: 830 2944 4915 5355 7348 7501 7660 7831 7921 Size: 2114 1971 440 1993 153 159 171 90 Cuts at: 7921 7975 8323 8775 9436 10630 11185 11379 12060 Size: 54 348 452 661 1194 555 194 681 Cuts at: 12060 12816 12885 13049 830 Size: 756 69 164 3478 Fragments arranged by size: 3478 2114 1993 1971 1194 756 681 661 555 452 440 348 194 171 164 159 153 90 69 54 PvuI CG_AT'CG Cuts at: 2001 10390 2001 Size: 8389 7308 PvuII CAG'CTG Cuts at: 3707 3781 5350 7093 7496 7976 10843 11057 11438 Size: 74 1569 1743 403 480 2867 214 381 Cuts at: 11438 12023 12221 12563 12682 3707 Size: 585 198 342 119 6722 Fragments arranged by size: 6722 2867 1743 1569 585 480 403 381 342 214 198 119 74 SacI G_AGCT'C Cuts at: 6774 8084 6774 Size: 1310 14387 SacII CC_GC'GG Cuts at: 11643 11643 Size: 15697 SalI G'TCGA_C Cuts at: 1936 13039 1936 Size: 11103 4594 SanDI GG'GwC_CC Cuts at: 12186 12186 Size: 15697 SapI GCTCTTCn'nnn_ Cuts at: 6552 9561 6552 Size: 3009 12688 SbfI CC_TGCA'GG Cuts at: 10630 12816 13049 10630 Size: 2186 233 13278 Fragments arranged by size: 13278 2186 233 ScaI AGT'ACT Cuts at: 11137 13945 15657 11137 Size: 2808 1712 11177 Fragments arranged by size: 11177 2808 1712 SexAI A'CCwGG_T Cuts at: 6127 6127 Size: 15697 SfcI C'TryA_G Cuts at: 826 1045 2512 2662 2940 3042 4300 4911 5351 Size: 219 1467 150 278 102 1258 611 440 Cuts at: 5351 7344 7497 7656 7827 7917 7971 8319 8771 Size: 1993 153 159 171 90 54 348 452 Cuts at: 8771 8838 9057 9432 10626 10989 11181 11332 11375 Size: 67 219 375 1194 363 192 151 43 Cuts at: 11375 12056 12812 12881 13045 14180 14861 15052 826 Size: 681 756 69 164 1135 681 191 1471 Fragments arranged by size: 1993 1471 1467 1258 1194 1135 756 681 681 611 452 440 375 363 348 278 219 219 192 191 171 164 159 153 151 150 102 90 69 67 54 43 SfoI GGC'GCC Cuts at: 6999 7706 8999 6999 Size: 707 1293 13697 Fragments arranged by size: 13697 1293 707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 15697 SgrAI Cr'CCGG_yG Cuts at: 1700 7875 9319 1700 Size: 6175 1444 8078 Fragments arranged by size: 8078 6175 1444 SmaI CCC'GGG Cuts at: 1872 2681 7219 11098 13024 1872 Size: 809 4538 3879 1926 4545 Fragments arranged by size: 4545 4538 3879 1926 809 SmlI C'TyrA_G Cuts at: 820 1017 1943 2523 3521 3960 4647 4895 5236 Size: 197 926 580 998 439 687 248 341 Cuts at: 5236 5649 7559 7928 9090 9336 10617 11505 12162 Size: 413 1910 369 1162 246 1281 888 657 Cuts at: 12162 12548 12788 13801 14702 14964 820 Size: 386 240 1013 901 262 1553 Fragments arranged by size: 1910 1553 1281 1162 1013 998 926 901 888 687 657 580 439 413 386 369 341 262 248 246 240 197 SnaBI TAC'GTA Cuts at: 493 13488 493 Size: 12995 2702 SpeI A'CTAG_T Cuts at: 152 11536 152 Size: 11384 4313 SphI G_CATG'C Cuts at: 1132 3217 3547 13055 1132 Size: 2085 330 9508 3774 Fragments arranged by size: 9508 3774 2085 330 SspI AAT'ATT Cuts at: 5 52 13621 5 Size: 47 13569 2081 Fragments arranged by size: 13569 2081 47 StuI AGG'CCT Cuts at: 1517 7861 8555 8854 9575 1517 Size: 6344 694 299 721 7639 Fragments arranged by size: 7639 6344 721 694 299 StyI C'CwwG_G Cuts at: 513 1065 2004 2612 3276 3997 5840 7734 7908 Size: 552 939 608 664 721 1843 1894 174 Cuts at: 7908 7955 7990 8085 8094 8898 9918 10728 10914 Size: 47 35 95 9 804 1020 810 186 Cuts at: 10914 11114 11875 13136 513 Size: 200 761 1261 3074 Fragments arranged by size: 3074 1894 1843 1261 1020 939 810 804 761 721 664 608 552 200 186 174 95 47 35 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2020 2188 2201 2721 2971 6196 7727 Size: 1255 440 168 13 520 250 3225 1531 Cuts at: 7727 8507 9403 10377 10490 10664 10790 10817 11235 Size: 780 896 974 113 174 126 27 418 Cuts at: 11235 11273 12116 12269 12600 12794 13724 13741 13894 Size: 38 843 153 331 194 930 17 153 Cuts at: 13894 14079 14524 325 Size: 185 445 1498 Fragments arranged by size: 3225 1531 1498 1255 974 930 896 843 780 520 445 440 418 331 250 194 185 174 168 153 153 126 113 38 27 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 9127 9197 11135 Size: 274 80 33 51 1443 7150 70 1938 Cuts at: 11135 13943 15655 96 Size: 2808 1712 138 Fragments arranged by size: 7150 2808 1938 1712 1443 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3381 3413 3721 3753 10352 10384 10773 10805 11962 Size: 32 308 32 6599 32 389 32 1157 Cuts at: 11962 11994 12461 12493 15657 15689 3381 Size: 32 467 32 3164 32 3389 Fragments arranged by size: 6599 3389 3164 1157 467 389 308 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4724 10892 11266 12781 1316 Size: 3408 6168 374 1515 4232 Fragments arranged by size: 6168 4232 3408 1515 374 XbaI T'CTAG_A Cuts at: 2753 2792 2825 5075 6640 6943 13033 2753 Size: 39 33 2250 1565 303 6090 5417 Fragments arranged by size: 6090 5417 2250 1565 303 39 33 XcmI CCAnnnn_n'nnnnTGG Cuts at: 7801 7941 8050 8260 8393 11173 12584 7801 Size: 140 109 210 133 2780 1411 10914 Fragments arranged by size: 10914 2780 1411 210 140 133 109 XhoI C'TCGA_G Cuts at: 1943 2523 3960 4647 11505 1943 Size: 580 1437 687 6858 6135 Fragments arranged by size: 6858 6135 1437 687 580 XmaI C'CCGG_G Cuts at: 1870 2679 7217 11096 13022 1870 Size: 809 4538 3879 1926 4545 Fragments arranged by size: 4545 4538 3879 1926 809 XmnI GAAnn'nnTTC Cuts at: 11352 13826 11352 Size: 2474 13223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4202 13505 276 Size: 53 83 186 936 2668 9303 2468 Fragments arranged by size: 9303 2668 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I FseI FspAI NsiI PacI PmlI RsrII SfiI SrfI SwaI