(Linear) (Six-base) MAPSORT of: KIEE2594.seq Check: 6642 from: 1 to: 10992 KIEE2594.orf With 182 enzymes: SgfI * January 6, 2016 09:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3265 6742 10992 Size: 3265 3477 4250 Fragments arranged by size: 4250 3477 3265 AatII G_ACGT'C Cuts at: 0 2199 10992 Size: 2199 8793 AccI GT'mk_AC Cuts at: 0 5099 7534 10992 Size: 5099 2435 3458 Fragments arranged by size: 5099 3458 2435 AclI AA'CG_TT Cuts at: 0 10838 10992 Size: 10838 154 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 392 1082 1103 2489 2734 3485 4019 Size: 149 243 690 21 1386 245 751 534 Cuts at: 4019 5426 5797 8453 8902 10058 10525 10643 10992 Size: 1407 371 2656 449 1156 467 118 349 Fragments arranged by size: 2656 1407 1386 1156 751 690 534 467 449 371 349 245 243 149 118 21 AfeI AGC'GCT Cuts at: 0 1378 2350 9347 10992 Size: 1378 972 6997 1645 Fragments arranged by size: 6997 1645 1378 972 AflIII A'CryG_T Cuts at: 0 3062 6025 6455 9603 10992 Size: 3062 2963 430 3148 1389 Fragments arranged by size: 3148 3062 2963 1389 430 AgeI A'CCGG_T Cuts at: 0 7314 9917 10992 Size: 7314 2603 1075 Fragments arranged by size: 7314 2603 1075 AleI CACnn'nnGTG Cuts at: 0 2683 6046 8128 10992 Size: 2683 3363 2082 2864 Fragments arranged by size: 3363 2864 2683 2082 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 9205 10119 10168 10992 Size: 9205 914 49 824 Fragments arranged by size: 9205 914 824 49 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2376 2408 3762 3794 10992 Size: 2376 32 1354 32 7198 Fragments arranged by size: 7198 2376 1354 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 771 946 4864 5257 5347 5404 5434 6765 Size: 771 175 3918 393 90 57 30 1331 Cuts at: 6765 7681 9514 9840 10533 10561 10992 Size: 916 1833 326 693 28 431 Fragments arranged by size: 3918 1833 1331 916 771 693 431 393 326 175 90 57 30 28 ApaI G_GGCC'C Cuts at: 0 6055 8066 9014 9399 10030 10634 10992 Size: 6055 2011 948 385 631 604 358 Fragments arranged by size: 6055 2011 948 631 604 385 358 ApaLI G'TGCA_C Cuts at: 0 173 6755 9886 10992 Size: 173 6582 3131 1106 Fragments arranged by size: 6582 3131 1106 173 ApoI r'AATT_y Cuts at: 0 107 1072 1081 1261 1291 3128 3922 3993 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GCCn_nnn'nGGC Cuts at: 0 6571 9670 10819 10992 Size: 6571 3099 1149 173 Fragments arranged by size: 6571 3099 1149 173 BglII A'GATC_T Cuts at: 0 4113 6235 6735 10880 10992 Size: 4113 2122 500 4145 112 Fragments arranged by size: 4145 4113 2122 500 112 BlpI GC'TnA_GC Cuts at: 0 5348 10992 Size: 5348 5644 Bme1580I G_kGCm'C Cuts at: 0 177 6055 6759 7283 8066 8149 9014 9194 Size: 177 5878 704 524 783 83 865 180 Cuts at: 9194 9399 9890 10030 10266 10634 10992 Size: 205 491 140 236 368 358 Fragments arranged by size: 5878 865 783 704 524 491 368 358 236 205 180 177 140 83 BmgBI CAC'GTC Cuts at: 0 3063 6028 6456 10992 Size: 3063 2965 428 4536 Fragments arranged by size: 4536 3063 2965 428 BmrI ACTGGGnnnn_n' Cuts at: 0 629 738 954 3271 3779 6399 6483 9181 Size: 629 109 216 2317 508 2620 84 2698 Cuts at: 9181 9189 9311 9815 10992 Size: 8 122 504 1177 Fragments arranged by size: 2698 2620 2317 1177 629 508 504 216 122 109 84 8 BmtI G_CTAG'C Cuts at: 0 779 1463 10992 Size: 779 684 9529 Fragments 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102 1385 1905 341 Cuts at: 5902 5905 6115 6118 7169 7172 7339 7531 7919 Size: 3 210 3 1051 3 167 192 388 Cuts at: 7919 9065 9347 9484 10022 10049 10992 Size: 1146 282 137 538 27 943 Fragments arranged by size: 1905 1417 1385 1146 1051 943 538 414 388 341 282 210 192 189 167 149 137 102 27 3 3 3 BseYI C'CCAG_C Cuts at: 0 1521 3238 5267 5397 6699 7304 7384 8059 Size: 1521 1717 2029 130 1302 605 80 675 Cuts at: 8059 8971 9013 9021 9124 9377 9429 9455 9953 Size: 912 42 8 103 253 52 26 498 Cuts at: 9953 10088 10115 10212 10393 10992 Size: 135 27 97 181 599 Fragments arranged by size: 2029 1717 1521 1302 912 675 605 599 498 253 181 135 130 103 97 80 52 42 27 26 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 932 1123 3696 6602 8210 8231 9428 9538 Size: 932 191 2573 2906 1608 21 1197 110 Cuts at: 9538 9541 9898 10079 10090 10585 10992 Size: 3 357 181 11 495 407 Fragments arranged by size: 2906 2573 1608 1197 932 495 407 357 191 181 110 21 11 3 BsiEI CG_ry'CG Cuts at: 0 5947 8385 10992 Size: 5947 2438 2607 Fragments arranged by size: 5947 2607 2438 BsiHKAI G_wGCw'C Cuts at: 0 177 4769 6079 6759 8053 8447 9890 10992 Size: 177 4592 1310 680 1294 394 1443 1102 Fragments arranged by size: 4592 1443 1310 1294 1102 680 394 177 BsmI GAATG_Cn' Cuts at: 0 7350 8001 8160 8202 10992 Size: 7350 651 159 42 2790 Fragments arranged by size: 7350 2790 651 159 42 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2222 2409 5040 7983 10584 10992 Size: 2222 187 2631 2943 2601 408 Fragments arranged by size: 2943 2631 2601 2222 408 187 Bsp1286I G_dGCh'C Cuts at: 0 177 545 615 992 4769 5768 6055 6079 Size: 177 368 70 377 3777 999 287 24 Cuts at: 6079 6759 7283 7480 7530 8023 8053 8066 8149 Size: 680 524 197 50 493 30 13 83 Cuts at: 8149 8404 8447 8741 9014 9194 9366 9399 9890 Size: 255 43 294 273 180 172 33 491 Cuts at: 9890 10030 10049 10266 10634 10992 Size: 140 19 217 368 358 Fragments arranged by size: 3777 999 680 524 493 491 377 368 368 358 294 287 273 255 217 197 180 177 172 140 83 70 50 43 33 30 24 19 13 BspEI T'CCGG_A Cuts at: 0 2394 10992 Size: 2394 8598 BspHI T'CATG_A Cuts at: 0 588 10970 10992 Size: 588 10382 22 Fragments arranged by size: 10382 588 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3265 3339 5073 5111 5919 6742 10230 10814 Size: 3265 74 1734 38 808 823 3488 584 Cuts at: 10814 10992 Size: 178 Fragments arranged by size: 3488 3265 1734 823 808 584 178 74 38 BsrBI CCG'CTC Cuts at: 0 390 525 702 5750 10992 Size: 390 135 177 5048 5242 Fragments arranged by size: 5242 5048 390 177 135 BsrDI GCAATG_nn' Cuts at: 0 7670 7838 8531 10979 10992 Size: 7670 168 693 2448 13 Fragments arranged by size: 7670 2448 693 168 13 BsrFI r'CCGG_y Cuts at: 0 5870 6198 6877 7314 8922 9917 10992 Size: 5870 328 679 437 1608 995 1075 Fragments arranged by size: 5870 1608 1075 995 679 437 328 BssSI C'ACGA_G Cuts at: 0 371 3625 4370 10992 Size: 371 3254 745 6622 Fragments arranged by size: 6622 3254 745 371 BstAPI GCAn_nnn'nTGC Cuts at: 0 946 5053 8197 8299 8629 9885 10533 10992 Size: 946 4107 3144 102 330 1256 648 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at: 9870 10484 10992 Size: 614 508 Fragments arranged by size: 5729 1436 1001 616 614 508 441 351 235 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 6516 8573 10992 Size: 6516 2057 2419 Fragments arranged by size: 6516 2419 2057 BtsI GCAGTG_nn' Cuts at: 0 628 879 888 1788 5336 5501 6263 6921 Size: 628 251 9 900 3548 165 762 658 Cuts at: 6921 7411 8198 8219 8300 10992 Size: 490 787 21 81 2692 Fragments arranged by size: 3548 2692 900 787 762 658 628 490 251 165 81 21 9 ClaI AT'CG_AT Cuts at: 0 3488 10992 Size: 3488 7504 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 7653 7688 10992 Size: 7653 35 3304 Fragments arranged by size: 7653 3304 35 DraI TTT'AAA Cuts at: 0 2978 3920 10992 Size: 2978 942 7072 Fragments arranged by size: 7072 2978 942 DraIII CAC_nnn'GTG Cuts at: 0 6933 9406 10992 Size: 6933 2473 1586 Fragments arranged by size: 6933 2473 1586 DrdI GACnn_nn'nnGTC Cuts at: 0 10330 10992 Size: 10330 662 EaeI y'GGCC_r Cuts at: 0 87 3968 6650 8261 8516 8639 8924 9337 Size: 87 3881 2682 1611 255 123 285 413 Cuts at: 9337 10131 10743 10992 Size: 794 612 249 Fragments arranged by size: 3881 2682 1611 794 612 413 285 255 249 123 87 EarI CTCTTCn'nnn_ Cuts at: 0 50 1118 1842 3905 4368 4547 5003 5521 Size: 50 1068 724 2063 463 179 456 518 Cuts at: 5521 5879 7556 10343 10992 Size: 358 1677 2787 649 Fragments arranged by size: 2787 2063 1677 1068 724 649 518 463 456 358 179 50 EcoICRI GAG'CTC Cuts at: 0 4767 6077 10992 Size: 4767 1310 4915 Fragments arranged by size: 4915 4767 1310 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 392 819 899 1082 1103 1898 2231 Size: 149 243 427 80 183 21 795 333 Cuts at: 2231 2489 2734 2953 3485 4019 5411 5426 5797 Size: 258 245 219 532 534 1392 15 371 Cuts at: 5797 6231 6532 6740 6836 7529 7575 7748 7817 Size: 434 301 208 96 693 46 173 69 Cuts at: 7817 8111 8453 8902 9189 9817 9876 10058 10525 Size: 294 342 449 287 628 59 182 467 Cuts at: 10525 10643 10684 10992 Size: 118 41 308 Fragments arranged by size: 1392 795 693 628 534 532 467 449 434 427 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4334 5590 8875 10992 Size: 2704 1630 1256 3285 2117 Fragments arranged by size: 3285 2704 2117 1630 1256 KasI G'GCGC_C Cuts at: 0 4992 5699 6992 10992 Size: 4992 707 1293 4000 Fragments arranged by size: 4992 4000 1293 707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3816 5230 7100 7288 10976 10992 Size: 3816 1414 1870 188 3688 16 Fragments arranged by size: 3816 3688 1870 1414 188 16 MscI TGG'CCA Cuts at: 0 3970 8641 10992 Size: 3970 4671 2351 Fragments arranged by size: 4671 3970 2351 MslI CAynn'nnrTG Cuts at: 0 2683 3812 4774 6046 8128 8371 9166 9187 Size: 2683 1129 962 1272 2082 243 795 21 Cuts at: 9187 9602 10992 Size: 415 1390 Fragments arranged by size: 2683 2082 1390 1272 1129 962 795 415 243 21 MspA1I CmG'CkG Cuts at: 0 815 1702 1776 3345 5088 5491 5827 5971 Size: 815 887 74 1569 1743 403 336 144 Cuts at: 5971 8182 8227 8484 8838 9052 9369 9433 9637 Size: 2211 45 257 354 214 317 64 204 Cuts at: 9637 9896 10018 10216 10558 10677 10992 Size: 259 122 198 342 119 315 Fragments arranged by size: 2211 1743 1569 887 815 403 354 342 336 317 315 259 257 214 204 198 144 122 119 74 64 45 NaeI GCC'GGC Cuts at: 0 5872 6879 8924 10992 Size: 5872 1007 2045 2068 Fragments arranged by size: 5872 2068 2045 1007 NarI GG'CG_CC Cuts at: 0 4993 5700 6993 10992 Size: 4993 707 1293 3999 Fragments arranged by size: 4993 3999 1293 707 NcoI C'CATG_G Cuts at: 0 5729 6080 9870 10992 Size: 5729 351 3790 1122 Fragments arranged by size: 5729 3790 1122 351 NgoMIV G'CCGG_C Cuts at: 0 5870 6877 8922 10992 Size: 5870 1007 2045 2070 Fragments arranged by size: 5870 2070 2045 1007 NheI G'CTAG_C Cuts at: 0 775 1459 10992 Size: 775 684 9533 Fragments arranged by size: 9533 775 684 NruI TCG'CGA Cuts at: 0 617 10992 Size: 617 10375 NspI r_CATG'y Cuts at: 0 1212 1542 9607 10992 Size: 1212 330 8065 1385 Fragments arranged by size: 8065 1385 1212 330 PasI CC'CwG_GG Cuts at: 0 6305 8359 8772 8799 9282 9629 10992 Size: 6305 2054 413 27 483 347 1363 Fragments arranged by size: 6305 2054 1363 483 413 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Cuts at: 0 939 2910 3350 5343 5496 5655 5826 5916 Size: 939 1971 440 1993 153 159 171 90 Cuts at: 5916 5970 6318 6770 7431 8625 9180 9374 10055 Size: 54 348 452 661 1194 555 194 681 Cuts at: 10055 10811 10880 10992 Size: 756 69 112 Fragments arranged by size: 1993 1971 1194 939 756 681 661 555 452 440 348 194 171 159 153 112 90 69 54 PvuI CG_AT'CG Cuts at: 0 8385 10992 Size: 8385 2607 PvuII CAG'CTG Cuts at: 0 1702 1776 3345 5088 5491 5971 8838 9052 Size: 1702 74 1569 1743 403 480 2867 214 Cuts at: 9052 9433 10018 10216 10558 10677 10992 Size: 381 585 198 342 119 315 Fragments arranged by size: 2867 1743 1702 1569 585 480 403 381 342 315 214 198 119 74 SacI G_AGCT'C Cuts at: 0 4769 6079 10992 Size: 4769 1310 4913 Fragments arranged by size: 4913 4769 1310 SacII CC_GC'GG Cuts at: 0 9638 10992 Size: 9638 1354 SanDI GG'GwC_CC Cuts at: 0 10181 10992 Size: 10181 811 SapI GCTCTTCn'nnn_ Cuts at: 0 4547 7556 10992 Size: 4547 3009 3436 Fragments arranged by size: 4547 3436 3009 SbfI CC_TGCA'GG Cuts at: 0 8625 10811 10992 Size: 8625 2186 181 Fragments arranged by size: 8625 2186 181 ScaI AGT'ACT Cuts at: 0 9132 10992 Size: 9132 1860 SexAI A'CCwGG_T Cuts at: 0 4122 10992 Size: 4122 6870 SfcI C'TryA_G Cuts at: 0 507 657 935 1037 2295 2906 3346 5339 Size: 507 150 278 102 1258 611 440 1993 Cuts at: 5339 5492 5651 5822 5912 5966 6314 6766 6833 Size: 153 159 171 90 54 348 452 67 Cuts at: 6833 7052 7427 8621 8984 9176 9327 9370 10051 Size: 219 375 1194 363 192 151 43 681 Cuts at: 10051 10807 10876 10992 Size: 756 69 116 Fragments arranged by size: 1993 1258 1194 756 681 611 507 452 440 375 363 348 278 219 192 171 159 153 151 150 116 102 90 69 67 54 43 SfoI GGC'GCC Cuts at: 0 4994 5701 6994 10992 Size: 4994 707 1293 3998 Fragments arranged by size: 4994 3998 1293 707 SgrAI Cr'CCGG_yG Cuts at: 0 5870 7314 10992 Size: 5870 1444 3678 Fragments arranged by size: 5870 3678 1444 SmaI CCC'GGG Cuts at: 0 676 5214 9093 10992 Size: 676 4538 3879 1899 Fragments arranged by size: 4538 3879 1899 676 SmlI C'TyrA_G Cuts at: 0 518 1516 1955 2642 2890 3231 3644 5554 Size: 518 998 439 687 248 341 413 1910 Cuts at: 5554 5923 7085 7331 8612 9500 10157 10543 10783 Size: 369 1162 246 1281 888 657 386 240 Cuts at: 10783 10992 Size: 209 Fragments arranged by size: 1910 1281 1162 998 888 687 657 518 439 413 386 369 341 248 246 240 209 SpeI A'CTAG_T Cuts at: 0 9531 10992 Size: 9531 1461 SphI G_CATG'C Cuts at: 0 1212 1542 10992 Size: 1212 330 9450 Fragments arranged by size: 9450 1212 330 StuI AGG'CCT Cuts at: 0 5856 6550 6849 7570 10992 Size: 5856 694 299 721 3422 Fragments arranged by size: 5856 3422 721 694 299 StyI C'CwwG_G Cuts at: 0 607 1271 1992 3835 5729 5903 5950 5985 Size: 607 664 721 1843 1894 174 47 35 Cuts at: 5985 6080 6089 6893 7913 8723 8909 9109 9870 Size: 95 9 804 1020 810 186 200 761 Cuts at: 9870 10992 Size: 1122 Fragments arranged by size: 1894 1843 1122 1020 810 804 761 721 664 607 200 186 174 95 47 35 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 15 183 196 716 966 4191 5722 6502 Size: 15 168 13 520 250 3225 1531 780 Cuts at: 6502 7398 8372 8485 8659 8785 8812 9230 9268 Size: 896 974 113 174 126 27 418 38 Cuts at: 9268 10111 10264 10595 10789 10992 Size: 843 153 331 194 203 Fragments arranged by size: 3225 1531 974 896 843 780 520 418 331 250 203 194 174 168 153 126 113 38 27 15 13 TatI w'GTAC_w Cuts at: 0 7122 7192 9130 10992 Size: 7122 70 1938 1862 Fragments arranged by size: 7122 1938 1862 70 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1376 1408 1716 1748 8347 8379 8768 8800 Size: 1376 32 308 32 6599 32 389 32 Cuts at: 8800 9957 9989 10456 10488 10992 Size: 1157 32 467 32 504 Fragments arranged by size: 6599 1376 1157 504 467 389 308 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2719 8887 9261 10776 10992 Size: 2719 6168 374 1515 216 Fragments arranged by size: 6168 2719 1515 374 216 XbaI T'CTAG_A Cuts at: 0 748 787 820 3070 4635 4938 10992 Size: 748 39 33 2250 1565 303 6054 Fragments arranged by size: 6054 2250 1565 748 303 39 33 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 5796 5936 6045 6255 6388 9168 10579 10992 Size: 5796 140 109 210 133 2780 1411 413 Fragments arranged by size: 5796 2780 1411 413 210 140 133 109 XhoI C'TCGA_G Cuts at: 0 518 1955 2642 9500 10992 Size: 518 1437 687 6858 1492 Fragments arranged by size: 6858 1492 1437 687 518 XmaI C'CCGG_G Cuts at: 0 674 5212 9091 10992 Size: 674 4538 3879 1901 Fragments arranged by size: 4538 3879 1901 674 XmnI GAAnn'nnTTC Cuts at: 0 9347 10992 Size: 9347 1645 ZraI GAC'GTC Cuts at: 0 2197 10992 Size: 2197 8795 Enzymes that do cut: AarI AatII AccI AclI AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AflII AhdI AscI AseI AsiSI BaeI BfrBI BsiWI BsrGI BssHII BstZ17I EagI EciI FseI FspI FspAI HpaI KpnI MfeI MluI NdeI NotI NsiI PacI PmeI PmlI PpiI RsrII SalI SfiI SgfI SnaBI SrfI SspI SwaI