(Circular) (Six-base) MAPSORT of: pFN21AE2800.seq Check: 5857 from: 1 to: 6196 pFN21AE2800 6196 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3539 1937 Size: 1602 4594 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3175 3477 4259 5643 5911 1632 Size: 1543 302 782 1384 268 1917 Fragments arranged by size: 1917 1543 1384 782 302 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 3204 5095 6072 1203 Size: 2001 1891 977 1327 Fragments arranged by size: 2001 1891 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 2764 4925 2764 Size: 2161 4035 AleI CACnn'nnGTG Cuts at: 1166 2501 1166 Size: 1335 4861 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6196 AlwNI CAG_nnn'CTG Cuts at: 2365 2414 2959 3313 3939 5511 2365 Size: 49 545 354 626 1572 3050 Fragments arranged by size: 3050 1572 626 545 354 49 ApaI G_GGCC'C Cuts at: 2914 2914 Size: 6196 ApaLI G'TGCA_C Cuts at: 4253 5409 4253 Size: 1156 5040 ApoI r'AATT_y Cuts at: 1493 2220 2387 3505 3731 3883 5992 1493 Size: 727 167 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 727 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 2073 3521 1870 Size: 73 130 1448 4545 Fragments arranged by size: 4545 1448 130 73 AvrII C'CTAG_G Cuts at: 2527 2527 Size: 6196 BamHI G'GATC_C Cuts at: 3526 3893 3526 Size: 367 5829 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3517 4972 618 Size: 325 217 627 1730 1455 1842 Fragments arranged by size: 1842 1730 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2157 2322 2522 2914 3515 1391 Size: 766 165 200 392 601 4072 Fragments arranged by size: 4072 766 601 392 200 165 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2203 3181 928 Size: 971 304 978 3943 Fragments arranged by size: 3943 978 971 304 BbvCI CC'TCA_GC Cuts at: 3248 3248 Size: 6196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2092 2126 2541 2575 4387 4421 1903 Size: 34 155 34 415 34 1812 34 3678 Fragments arranged by size: 3678 1812 415 155 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BfrBI ATG'CAT Cuts at: 3176 3176 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6191 6191 Size: 6196 BlpI GC'TnA_GC Cuts at: 2321 3613 2321 Size: 1292 4904 Bme1580I G_kGCm'C Cuts at: 1790 2016 2914 4257 5413 1790 Size: 226 898 1343 1156 2573 Fragments arranged by size: 2573 1343 1156 898 226 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3041 3927 4885 Size: 416 53 83 399 24 1624 886 958 Cuts at: 4885 442 Size: 1753 Fragments arranged by size: 1753 1624 958 886 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4856 1239 Size: 3617 2579 Bpu10I CC'TnA_GC Cuts at: 3248 3248 Size: 6196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3465 4321 5186 5484 3465 Size: 856 865 298 4177 Fragments arranged by size: 4177 865 856 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4859 882 Size: 617 3360 2219 Fragments arranged by size: 3360 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3987 493 Size: 711 2783 2702 Fragments arranged by size: 2783 2702 711 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4003 4385 5020 275 Size: 53 83 186 936 2470 382 635 1451 Fragments arranged by size: 2470 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2809 4629 5027 5301 5448 5859 2809 Size: 1820 398 274 147 411 3146 Fragments arranged by size: 3146 1820 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5971 Fragments arranged by size: 5971 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 2866 1186 Size: 1618 62 4516 Fragments arranged by size: 4516 1618 62 BseYI C'CCAG_C Cuts at: 2176 2728 2751 5399 5895 2176 Size: 552 23 2648 496 2477 Fragments arranged by size: 2648 2477 552 496 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3447 1163 Size: 573 1711 3912 Fragments arranged by size: 3912 1711 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 3646 4407 5435 1540 Size: 275 186 105 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 105 BsiHKAI G_wGCw'C Cuts at: 2061 4257 4342 5413 2061 Size: 2196 85 1071 2844 Fragments arranged by size: 2844 2196 1071 85 BsmI GAATG_Cn' Cuts at: 2093 3707 3800 2093 Size: 1614 93 4489 Fragments arranged by size: 4489 1614 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2834 2834 Size: 6196 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2061 2157 2322 2522 2914 3515 Size: 399 226 45 96 165 200 392 601 Cuts at: 3515 4257 4342 5413 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 399 392 226 200 165 96 85 45 BspEI T'CCGG_A Cuts at: 5859 5859 Size: 6196 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 2681 3551 844 Size: 1337 500 870 3489 Fragments arranged by size: 3489 1337 870 500 BsrBI CCG'CTC Cuts at: 1394 1823 2072 4083 1394 Size: 429 249 2011 3507 Fragments arranged by size: 3507 2011 429 249 BsrDI GCAATG_nn' Cuts at: 65 1248 3291 4691 4865 65 Size: 1183 2043 1400 174 1396 Fragments arranged by size: 2043 1400 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4840 5027 1700 Size: 110 3030 187 2869 Fragments arranged by size: 3030 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 3313 794 Size: 2519 3677 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstXI CCAn_nnnn'nTGG Cuts at: 2503 2747 2503 Size: 244 5952 BstYI r'GATC_y Cuts at: 1972 3463 3526 3893 4278 4295 5747 5833 5845 Size: 1491 63 367 385 17 1452 86 12 Cuts at: 5845 6191 1972 Size: 346 1977 Fragments arranged by size: 1977 1491 1452 385 367 346 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2141 2141 Size: 6196 BtgI C'CryG_G Cuts at: 513 1065 2004 2799 513 Size: 552 939 795 3910 Fragments arranged by size: 3910 939 795 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2386 3385 487 Size: 683 56 1160 999 3298 Fragments arranged by size: 3298 1160 999 683 56 BtsI GCAGTG_nn' Cuts at: 3138 3302 3712 4506 4526 3138 Size: 164 410 794 20 4808 Fragments arranged by size: 4808 794 410 164 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2401 2436 529 Size: 35 1837 35 4289 Fragments arranged by size: 4289 1837 35 35 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2370 3417 Size: 54 1425 50 275 18 219 321 1047 Cuts at: 3417 3643 4532 6161 8 Size: 226 889 1629 43 Fragments arranged by size: 1629 1425 1047 889 321 275 226 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2636 2654 2892 4126 1353 Size: 1283 18 238 1234 3423 Fragments arranged by size: 3423 1283 1234 238 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3175 3477 4259 4856 5643 5911 1239 Size: 393 1543 302 782 597 787 268 1524 Fragments arranged by size: 1543 1524 787 782 597 393 302 268 EcoO109I rG'GnC_Cy Cuts at: 2911 2956 2911 Size: 45 6151 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2514 2665 2514 Size: 151 6045 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 5343 1678 Size: 3665 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2750 2782 2965 2997 3088 Size: 32 135 32 1178 32 183 32 91 Cuts at: 3088 3120 3455 3487 4837 4869 4911 4943 1373 Size: 32 335 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 1178 335 183 135 91 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3540 3786 669 Size: 988 281 1143 459 246 3079 Fragments arranged by size: 3079 1143 988 459 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6196 HpaI GTT'AAC Cuts at: 3786 3786 Size: 6196 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5310 5494 1305 Size: 4005 184 2007 Fragments arranged by size: 4005 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 3419 10 Size: 54 1425 343 540 1047 2787 Fragments arranged by size: 2787 1425 1047 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2501 3164 3203 3404 4154 4513 Size: 648 843 492 663 39 201 750 359 Cuts at: 4513 4672 518 Size: 159 2042 Fragments arranged by size: 2042 843 750 663 648 492 359 201 159 39 MspA1I CmG'CkG Cuts at: 1606 3612 4289 5437 5682 1606 Size: 2006 677 1148 245 2120 Fragments arranged by size: 2120 2006 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4705 Fragments arranged by size: 4705 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NruI TCG'CGA Cuts at: 2085 2085 Size: 6196 NsiI A_TGCA'T Cuts at: 3178 3178 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 2464 3208 3554 5099 1132 Size: 97 1235 744 346 1545 2229 Fragments arranged by size: 2229 1545 1235 744 346 97 PasI CC'CwG_GG Cuts at: 1348 2359 1348 Size: 1011 5185 PciI A'CATG_T Cuts at: 3204 5095 3204 Size: 1891 4305 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6196 PsiI TTA'TAA Cuts at: 2295 3766 2295 Size: 1471 4725 PspOMI G'GGCC_C Cuts at: 2910 2910 Size: 6196 PstI C_TGCA'G Cuts at: 830 2316 3133 3309 3548 830 Size: 1486 817 176 239 3478 Fragments arranged by size: 3478 1486 817 239 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 RsrII CG'GwC_CG Cuts at: 2067 2067 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SapI GCTCTTCn'nnn_ Cuts at: 2636 2636 Size: 6196 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 2847 4444 6156 2847 Size: 1597 1712 2887 Fragments arranged by size: 2887 1712 1597 SexAI A'CCwGG_T Cuts at: 2247 2247 Size: 6196 SfcI C'TryA_G Cuts at: 826 1045 2312 2440 3129 3305 3544 4679 5360 Size: 219 1267 128 689 176 239 1135 681 Cuts at: 5360 5551 826 Size: 191 1471 Fragments arranged by size: 1471 1267 1135 689 681 239 219 191 176 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3480 4300 5201 5463 820 Size: 197 926 1537 820 901 262 1553 Fragments arranged by size: 1553 1537 926 901 820 262 197 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 2004 2527 3346 3635 513 Size: 552 939 523 819 289 3074 Fragments arranged by size: 3074 939 819 552 523 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 3023 3154 4223 4240 4393 4578 Size: 1255 633 810 131 1069 17 153 185 Cuts at: 4578 5023 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1069 810 633 445 185 153 131 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2845 4442 6154 Size: 274 80 33 51 1443 868 1597 1712 Cuts at: 6154 96 Size: 138 Fragments arranged by size: 1712 1597 1443 868 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6156 6188 6156 Size: 32 6164 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 2383 3532 2383 Size: 1149 5047 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2684 2684 Size: 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 2858 4325 2858 Size: 1467 4729 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI SfiI SfoI SrfI SwaI