(Linear) (Six-base) MAPSORT of: KIEE2800.seq Check: 9108 from: 1 to: 1491 KIEE2800.orf With 182 enzymes: SgfI * August 16, 2021 14:16 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1170 1491 Size: 1170 321 AflIII A'CryG_T Cuts at: 0 1199 1491 Size: 1199 292 AhdI GACnn_n'nnGTC Cuts at: 0 759 1491 Size: 759 732 AleI CACnn'nnGTG Cuts at: 0 496 1491 Size: 496 995 AlwNI CAG_nnn'CTG Cuts at: 0 360 409 954 1308 1491 Size: 360 49 545 354 183 Fragments arranged by size: 545 360 354 183 49 ApaI G_GGCC'C Cuts at: 0 909 1491 Size: 909 582 ApoI r'AATT_y Cuts at: 0 215 382 1491 Size: 215 167 1109 Fragments arranged by size: 1109 215 167 AvaI C'yCGr_G Cuts at: 0 68 1491 Size: 68 1423 AvrII C'CTAG_G Cuts at: 0 522 1491 Size: 522 969 BanII G_rGCy'C Cuts at: 0 152 317 517 909 1491 Size: 152 165 200 392 582 Fragments arranged by size: 582 392 200 165 152 BbsI GAAGACnn'nnnn_ Cuts at: 0 198 1176 1491 Size: 198 978 315 Fragments arranged by size: 978 315 198 BbvCI CC'TCA_GC Cuts at: 0 1243 1491 Size: 1243 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 87 121 536 570 1491 Size: 87 34 415 34 921 Fragments arranged by size: 921 415 87 34 34 BfrBI ATG'CAT Cuts at: 0 1171 1491 Size: 1171 320 BlpI GC'TnA_GC Cuts at: 0 316 1491 Size: 316 1175 Bme1580I G_kGCm'C Cuts at: 0 11 909 1491 Size: 11 898 582 Fragments arranged by size: 898 582 11 BmrI ACTGGGnnnn_n' Cuts at: 0 1036 1491 Size: 1036 455 Bpu10I CC'TnA_GC Cuts at: 0 1243 1491 Size: 1243 248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1460 1491 Size: 1460 31 BsaWI w'CCGG_w Cuts at: 0 804 1491 Size: 804 687 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 799 861 1491 Size: 799 62 630 Fragments arranged by size: 799 630 62 BseYI C'CCAG_C Cuts at: 0 171 723 746 1491 Size: 171 552 23 745 Fragments arranged by size: 745 552 171 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1442 1491 Size: 1442 49 BsiEI CG_ry'CG Cuts at: 0 101 1491 Size: 101 1390 BsiHKAI G_wGCw'C Cuts at: 0 56 1491 Size: 56 1435 BsmI GAATG_Cn' Cuts at: 0 88 1491 Size: 88 1403 BsmBI CGTCTCn'nnnn_ Cuts at: 0 829 1491 Size: 829 662 Bsp1286I G_dGCh'C Cuts at: 0 11 56 152 317 517 909 1491 Size: 11 45 96 165 200 392 582 Fragments arranged by size: 582 392 200 165 96 45 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 176 676 1491 Size: 176 500 815 Fragments arranged by size: 815 500 176 BsrBI CCG'CTC Cuts at: 0 67 1491 Size: 67 1424 BsrDI GCAATG_nn' Cuts at: 0 1286 1491 Size: 1286 205 BstAPI GCAn_nnn'nTGC Cuts at: 0 1308 1491 Size: 1308 183 BstXI CCAn_nnnn'nTGG Cuts at: 0 498 742 1491 Size: 498 244 749 Fragments arranged by size: 749 498 244 BstYI r'GATC_y Cuts at: 0 1458 1491 Size: 1458 33 Bsu36I CC'TnA_GG Cuts at: 0 136 1491 Size: 136 1355 BtgI C'CryG_G Cuts at: 0 794 1491 Size: 794 697 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 381 1380 1491 Size: 381 999 111 Fragments arranged by size: 999 381 111 BtsI GCAGTG_nn' Cuts at: 0 1133 1297 1491 Size: 1133 164 194 Fragments arranged by size: 1133 194 164 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 396 431 1491 Size: 396 35 1060 Fragments arranged by size: 1060 396 35 EaeI y'GGCC_r Cuts at: 0 44 365 1412 1491 Size: 44 321 1047 79 Fragments arranged by size: 1047 321 79 44 EarI CTCTTCn'nnn_ Cuts at: 0 631 649 887 1491 Size: 631 18 238 604 Fragments arranged by size: 631 604 238 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1170 1491 Size: 1170 321 EcoO109I rG'GnC_Cy Cuts at: 0 906 951 1491 Size: 906 45 540 Fragments arranged by size: 906 540 45 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 509 660 1491 Size: 509 151 831 Fragments arranged by size: 831 509 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 745 777 960 992 1083 1115 1450 1482 Size: 745 32 183 32 91 32 335 32 Cuts at: 1482 1491 Size: 9 Fragments arranged by size: 745 335 183 91 32 32 32 32 9 HincII GTy'rAC Cuts at: 0 1076 1491 Size: 1076 415 MscI TGG'CCA Cuts at: 0 367 1414 1491 Size: 367 1047 77 Fragments arranged by size: 1047 367 77 MslI CAynn'nnrTG Cuts at: 0 496 1159 1198 1399 1491 Size: 496 663 39 201 92 Fragments arranged by size: 663 496 201 92 39 NruI TCG'CGA Cuts at: 0 80 1491 Size: 80 1411 NsiI A_TGCA'T Cuts at: 0 1173 1491 Size: 1173 318 NspI r_CATG'y Cuts at: 0 459 1203 1491 Size: 459 744 288 Fragments arranged by size: 744 459 288 PasI CC'CwG_GG Cuts at: 0 354 1491 Size: 354 1137 PciI A'CATG_T Cuts at: 0 1199 1491 Size: 1199 292 PsiI TTA'TAA Cuts at: 0 290 1491 Size: 290 1201 PspOMI G'GGCC_C Cuts at: 0 905 1491 Size: 905 586 PstI C_TGCA'G Cuts at: 0 311 1128 1304 1491 Size: 311 817 176 187 Fragments arranged by size: 817 311 187 176 RsrII CG'GwC_CG Cuts at: 0 62 1491 Size: 62 1429 SapI GCTCTTCn'nnn_ Cuts at: 0 631 1491 Size: 631 860 ScaI AGT'ACT Cuts at: 0 842 1491 Size: 842 649 SexAI A'CCwGG_T Cuts at: 0 242 1491 Size: 242 1249 SfcI C'TryA_G Cuts at: 0 307 435 1124 1300 1491 Size: 307 128 689 176 191 Fragments arranged by size: 689 307 191 176 128 SmlI C'TyrA_G Cuts at: 0 1475 1491 Size: 1475 16 StyI C'CwwG_G Cuts at: 0 522 1341 1491 Size: 522 819 150 Fragments arranged by size: 819 522 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 208 1018 1149 1491 Size: 208 810 131 342 Fragments arranged by size: 810 342 208 131 TatI w'GTAC_w Cuts at: 0 840 1491 Size: 840 651 XbaI T'CTAG_A Cuts at: 0 378 1491 Size: 378 1113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 679 1491 Size: 679 812 XmnI GAAnn'nnTTC Cuts at: 0 853 1491 Size: 853 638 Enzymes that do cut: AcuI AflIII AhdI AleI AlwNI ApaI ApoI AvaI AvrII BanII BbsI BbvCI BcgI BfrBI BlpI Bme1580I BmrI Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII MscI MslI NruI NsiI NspI PasI PciI PsiI PspOMI PstI RsrII SapI ScaI SexAI SfcI SmlI StyI TaqII TatI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI BanI BbeI BciVI BclI BglI BglII BmgBI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BsiWI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI PacI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI PvuII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XhoI XmaI ZraI