################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpmI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 466 GTCGAC GT^[AC][GT]AC AcyI 256 GGCGTC G[GA]^CG[CT]C AlwNI 1627 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 198 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 680 GGATCC G^GATCC BanI 1543 GGTGCC G^G[CT][GA]CC BbsI 1201 GAAGAC GAAGAC(2/6) BcgI 468 CGACAATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 117 GCTGAGC GC^T[ACGT]AGC Bpu1102I 117 GCTGAGC GC^T[ACGT]AGC BpuAI 1201 GAAGAC GAAGAC(2/6) BsaHI 256 GGCGTC G[GA]^CG[CT]C BseRI 1746 GAGGAG GAGGAG(10/8) BsmI 1584 GAATGC GAATGC(1/-1) BspMI 1185 GCAGGT ACCTGC(4/8) BsrDI 122 GCAATG GCAATG(2/0) BssSI 1345 CACGAG CACGAG(-5/-1) CelII 117 GCTGAGC GC^T[ACGT]AGC EcoNI 1395 CCTTGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1 ATGCAT ATGCA^T Hin1I 256 GGCGTC G[GA]^CG[CT]C MaeII 12 ACGT A^CGT MseI 1127 TTAA T^TAA MspA1I 1627 CAGCTG C[AC]G^C[GT]G NsiI 1 ATGCAT ATGCA^T NspI 177 GCATGC [GA]CATG^[CT] PflMI 273 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1627 CAGCTG CAG^CTG SalI 466 GTCGAC G^TCGAC SphI 177 GCATGC GCATG^C TaqI 467 TCGA T^CGA TfiI 170 GAATC G^A[AT]TC Tru9I 1127 TTAA T^TAA TthHB8I 467 TCGA T^CGA Van91I 273 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 198 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BsrFI 235 ACCGGC [GA]^CCGG[CT] 694 GCCGGT BstF5I 61 GGATG GGATG(2/0) 153 GGATG BstYI 680 GGATCC [GA]^GATC[CT] 1654 GGATCT Cfr10I 235 ACCGGC [GA]^CCGG[CT] 694 GCCGGT FokI 61 GGATG GGATG(9/13) 153 GGATG HgaI 257 GCGTC GACGC(5/10) 1751 GCGTC HindIII 340 AAGCTT A^AGCTT 1531 AAGCTT HphI 1278 TCACC GGTGA(8/7) 1386 TCACC MboII 1201 GAAGA GAAGA(8/7) 1657 TCTTC MflI 680 GGATCC [GA]^GATC[CT] 1654 GGATCT SfaNI 3 GCATC GCATC(5/9) 158 GCATC StuI 1133 AGGCCT AGG^CCT 1218 AGGCCT XhoII 680 GGATCC [GA]^GATC[CT] 1654 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI AlwI ApaI AvaII BalI BanII BbvI BcnI BglI BmyI BsaI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HincII HindII HinfI HpaII ItaI MboI MluNI MnlI MscI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI XcmI