(Circular) (Six-base) MAPSORT of: pFN21AE2798.seq Check: 677 from: 1 to: 6475 pFN21AE2798 6475 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3181 3181 Size: 6475 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2472 3818 1937 Size: 535 1346 4594 Fragments arranged by size: 4594 1346 535 Acc65I G'GTAC_C Cuts at: 3796 3796 Size: 6475 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4538 5922 6190 1632 Size: 2906 1384 268 1917 Fragments arranged by size: 2906 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 5374 6351 1203 Size: 4171 977 1327 Fragments arranged by size: 4171 1327 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6475 AlwNI CAG_nnn'CTG Cuts at: 3637 4218 5790 3637 Size: 581 1572 4322 Fragments arranged by size: 4322 1572 581 ApaI G_GGCC'C Cuts at: 2059 2635 2743 2905 2959 2059 Size: 576 108 162 54 5575 Fragments arranged by size: 5575 576 162 108 54 ApaLI G'TGCA_C Cuts at: 4532 5688 4532 Size: 1156 5319 ApoI r'AATT_y Cuts at: 1493 3784 4010 4162 6271 1493 Size: 2291 226 152 2109 1697 Fragments arranged by size: 2291 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 3800 1870 Size: 73 1857 4545 Fragments arranged by size: 4545 1857 73 BamHI G'GATC_C Cuts at: 2685 3805 4172 2685 Size: 1120 367 4988 Fragments arranged by size: 4988 1120 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3548 3796 5251 618 Size: 325 217 627 1761 248 1455 1842 Fragments arranged by size: 1842 1761 1455 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2059 2274 2635 2743 2905 2959 3794 1391 Size: 668 215 361 108 162 54 835 4072 Fragments arranged by size: 4072 835 668 361 215 162 108 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3213 928 Size: 971 1314 4190 Fragments arranged by size: 4190 1314 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2462 2496 4666 4700 1903 Size: 34 50 34 441 34 2170 34 3678 Fragments arranged by size: 3678 2170 441 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4367 5577 4367 Size: 1210 5265 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2241 2435 3707 Size: 107 122 71 631 605 569 194 1272 Cuts at: 3707 5086 136 Size: 1379 1525 Fragments arranged by size: 1525 1379 1272 631 605 569 194 122 107 71 BglII A'GATC_T Cuts at: 6470 6470 Size: 6475 BlpI GC'TnA_GC Cuts at: 2123 3892 2123 Size: 1769 4706 Bme1580I G_kGCm'C Cuts at: 1790 2059 2635 2743 2905 2959 3553 4536 5692 Size: 269 576 108 162 54 594 983 1156 Cuts at: 5692 1790 Size: 2573 Fragments arranged by size: 2573 1156 983 594 576 269 162 108 54 BmgBI CAC'GTC Cuts at: 1318 2018 1318 Size: 700 5775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2060 3459 4206 Size: 416 53 83 399 24 643 1399 747 Cuts at: 4206 5164 442 Size: 958 1753 Fragments arranged by size: 1753 1399 958 747 643 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5135 1239 Size: 3896 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4600 5465 5763 4600 Size: 865 298 5312 Fragments arranged by size: 5312 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2648 2810 3026 5138 882 Size: 617 1149 162 216 2112 2219 Fragments arranged by size: 2219 2112 1149 617 216 162 BsaAI yAC'GTr Cuts at: 493 1204 4266 493 Size: 711 3062 2702 Fragments arranged by size: 3062 2702 711 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 4282 4664 5299 Size: 53 83 186 936 729 2020 382 635 Cuts at: 5299 275 Size: 1451 Fragments arranged by size: 2020 1451 936 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4908 5306 5580 5727 6138 4908 Size: 398 274 147 411 5245 Fragments arranged by size: 5245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6250 Fragments arranged by size: 6250 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3766 1186 Size: 2580 3895 BseYI C'CCAG_C Cuts at: 2504 2558 2612 2990 3302 3464 5678 6174 2504 Size: 54 54 378 312 162 2214 496 2805 Fragments arranged by size: 2805 2214 496 378 312 162 54 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5902 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3925 4686 5714 1540 Size: 275 186 1924 761 1028 2301 Fragments arranged by size: 2301 1924 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4536 4621 5692 4536 Size: 85 1071 5319 Fragments arranged by size: 5319 1071 85 BsmI GAATG_Cn' Cuts at: 3595 3986 4079 3595 Size: 391 93 5991 Fragments arranged by size: 5991 391 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2274 2635 2743 2905 2959 3553 Size: 399 269 215 361 108 162 54 594 Cuts at: 3553 3794 4536 4621 5692 1391 Size: 241 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 594 399 361 269 241 215 162 108 85 54 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 4364 6094 4364 Size: 1730 4745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3181 3830 844 Size: 2337 649 3489 Fragments arranged by size: 3489 2337 649 BsrBI CCG'CTC Cuts at: 1394 1823 4362 1394 Size: 429 2539 3507 Fragments arranged by size: 3507 2539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2134 4970 5144 65 Size: 1183 886 2836 174 1396 Fragments arranged by size: 2836 1396 1183 886 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2240 2699 5119 5306 1700 Size: 110 430 459 2420 187 2869 Fragments arranged by size: 2869 2420 459 430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 3350 4535 5547 3350 Size: 1185 1012 4278 Fragments arranged by size: 4278 1185 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6475 BstXI CCAn_nnnn'nTGG Cuts at: 2318 2861 3464 3563 2318 Size: 543 603 99 5230 Fragments arranged by size: 5230 603 543 99 BstYI r'GATC_y Cuts at: 1972 2685 3659 3805 4172 4557 4574 6026 6112 Size: 713 974 146 367 385 17 1452 86 Cuts at: 6112 6124 6470 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 974 713 385 367 346 146 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2278 2311 2353 2638 2800 3016 3112 Size: 552 1213 33 42 285 162 216 96 Cuts at: 3112 3124 513 Size: 12 3864 Fragments arranged by size: 3864 1213 552 285 216 162 96 42 33 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 4868 Fragments arranged by size: 4868 868 683 56 BtsI GCAGTG_nn' Cuts at: 3991 4785 4805 3991 Size: 794 20 5661 Fragments arranged by size: 5661 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2261 3215 3431 5482 809 Size: 507 945 954 216 2051 1802 Fragments arranged by size: 2051 1802 954 945 507 216 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2350 2461 Size: 54 1425 50 275 18 243 277 111 Cuts at: 2461 2515 2569 2623 2677 2731 2785 2839 2893 Size: 54 54 54 54 54 54 54 54 Cuts at: 2893 2947 3001 3055 3109 3163 3271 3379 3487 Size: 54 54 54 54 54 108 108 108 Cuts at: 3487 3541 3565 3922 4811 6440 8 Size: 54 24 357 889 1629 43 Fragments arranged by size: 1629 1425 889 357 277 275 243 111 108 108 108 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3922 1537 Size: 275 2110 4090 Fragments arranged by size: 4090 2110 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4405 1353 Size: 3052 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5062 5436 5582 387 Size: 1064 138 3473 374 146 1280 Fragments arranged by size: 3473 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4538 5135 5922 6190 1239 Size: 393 2906 597 787 268 1524 Fragments arranged by size: 2906 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3404 3404 Size: 6475 EcoO109I rG'GnC_Cy Cuts at: 2055 2056 2152 2631 2739 2901 2955 3708 2055 Size: 1 96 479 108 162 54 753 4822 Fragments arranged by size: 4822 753 479 162 108 96 54 1 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3678 3678 Size: 6475 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 5622 1678 Size: 3944 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5116 5148 5190 5222 1373 Size: 32 135 32 3544 32 42 32 2626 Fragments arranged by size: 3544 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 2473 3499 3607 3718 3819 Size: 988 281 424 111 1026 108 111 101 Cuts at: 3819 4065 669 Size: 246 3079 Fragments arranged by size: 3079 1026 988 424 281 246 111 111 108 101 HindIII A'AGCT_T Cuts at: 748 2345 3536 748 Size: 1597 1191 3687 Fragments arranged by size: 3687 1597 1191 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KpnI G_GTAC'C Cuts at: 3800 3800 Size: 6475 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 3361 5589 5773 1305 Size: 927 1129 2228 184 2007 Fragments arranged by size: 2228 2007 1129 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2352 2463 2517 2571 2625 Size: 54 1425 343 520 111 54 54 54 Cuts at: 2625 2679 2733 2787 2841 2895 2949 3003 3057 Size: 54 54 54 54 54 54 54 54 Cuts at: 3057 3165 3273 3381 3489 3543 3567 10 Size: 108 108 108 108 54 24 2918 Fragments arranged by size: 2918 1425 520 343 111 108 108 108 108 54 54 54 54 54 54 54 54 54 54 54 54 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 4433 4792 4951 518 Size: 648 3267 359 159 2042 Fragments arranged by size: 3267 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3634 3891 4568 5716 5961 1606 Size: 2028 257 677 1148 245 2120 Fragments arranged by size: 2120 2028 1148 677 257 245 NcoI C'CATG_G Cuts at: 513 1065 2311 2353 2638 2800 3016 3112 3124 Size: 552 1246 42 285 162 216 96 12 Cuts at: 3124 513 Size: 3864 Fragments arranged by size: 3864 1246 552 285 216 162 96 42 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 2186 3833 5378 1132 Size: 97 957 1647 1545 2229 Fragments arranged by size: 2229 1647 1545 957 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 1108 Size: 1176 5299 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PspOMI G'GGCC_C Cuts at: 2055 2631 2739 2901 2955 2055 Size: 576 108 162 54 5575 Fragments arranged by size: 5575 576 162 108 54 PstI C_TGCA'G Cuts at: 830 2485 2539 2593 3588 3827 830 Size: 1655 54 54 995 239 3478 Fragments arranged by size: 3478 1655 995 239 54 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 3634 3634 Size: 6475 SalI G'TCGA_C Cuts at: 1936 2471 3817 1936 Size: 535 1346 4594 Fragments arranged by size: 4594 1346 535 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 2481 2535 2589 3584 3823 4958 5639 Size: 219 1436 54 54 995 239 1135 681 Cuts at: 5639 5830 826 Size: 191 1471 Fragments arranged by size: 1471 1436 1135 995 681 239 219 191 54 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 3802 1872 Size: 1930 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4579 5480 5742 820 Size: 197 926 2636 901 262 1553 Fragments arranged by size: 2636 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 2186 3833 1132 Size: 1054 1647 3774 Fragments arranged by size: 3774 1647 1054 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 3140 3225 1517 Size: 1623 85 4767 Fragments arranged by size: 4767 1623 85 StyI C'CwwG_G Cuts at: 513 1065 2311 2353 2638 2800 3016 3112 3124 Size: 552 1246 42 285 162 216 96 12 Cuts at: 3124 3220 3436 3490 3544 3914 513 Size: 96 216 54 54 370 3074 Fragments arranged by size: 3074 1246 552 370 285 216 216 162 96 96 54 54 42 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3703 4502 4519 4672 4857 5302 325 Size: 1255 2123 799 17 153 185 445 1498 Fragments arranged by size: 2123 1498 1255 799 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4721 6433 96 Size: 274 80 33 51 1443 2744 1712 138 Fragments arranged by size: 2744 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2181 2213 3513 3545 6435 6467 2181 Size: 32 1300 32 2890 32 2189 Fragments arranged by size: 2890 2189 1300 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2858 3560 3638 2858 Size: 702 78 5695 Fragments arranged by size: 5695 702 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 3800 1870 Size: 1930 4545 XmnI GAAnn'nnTTC Cuts at: 2207 4604 2207 Size: 2397 4078 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI