(Circular) (Six-base) MAPSORT of: pFN21AE2779.seq Check: 5602 from: 1 to: 7132 pFN21AE2779 7132 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4942 278 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4475 1937 Size: 2538 4594 Acc65I G'GTAC_C Cuts at: 2705 4453 2705 Size: 1748 5384 AclI AA'CG_TT Cuts at: 1563 5259 5632 1563 Size: 3696 373 3063 Fragments arranged by size: 3696 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2158 4107 4271 4331 5195 6579 6847 1632 Size: 526 1949 164 60 864 1384 268 1917 Fragments arranged by size: 1949 1917 1384 864 526 268 164 60 AfeI AGC'GCT Cuts at: 2348 3527 2348 Size: 1179 5953 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6935 AflIII A'CryG_T Cuts at: 1203 6031 7008 1203 Size: 4828 977 1327 Fragments arranged by size: 4828 1327 977 AgeI A'CCGG_T Cuts at: 5963 5963 Size: 7132 AhdI GACnn_n'nnGTC Cuts at: 5861 5861 Size: 7132 AleI CACnn'nnGTG Cuts at: 1166 4361 1166 Size: 3195 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7132 AlwNI CAG_nnn'CTG Cuts at: 4196 4875 6447 4196 Size: 679 1572 4881 Fragments arranged by size: 4881 1572 679 ApaLI G'TGCA_C Cuts at: 5189 6345 5189 Size: 1156 5976 ApoI r'AATT_y Cuts at: 1493 3412 3654 4052 4289 4441 4667 4819 6928 Size: 1919 242 398 237 152 226 152 2109 Cuts at: 6928 1493 Size: 1697 Fragments arranged by size: 2109 1919 1697 398 242 237 226 152 152 AseI AT'TA_AT Cuts at: 160 1032 2514 3284 3944 5686 160 Size: 872 1482 770 660 1742 1606 Fragments arranged by size: 1742 1606 1482 872 770 660 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 AvaI C'yCGr_G Cuts at: 1870 1943 4457 1870 Size: 73 2514 4545 Fragments arranged by size: 4545 2514 73 BamHI G'GATC_C Cuts at: 2393 4462 4829 2393 Size: 2069 367 4696 Fragments arranged by size: 4696 2069 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2705 3223 4453 5908 Size: 325 217 627 369 549 518 1230 1455 Cuts at: 5908 618 Size: 1842 Fragments arranged by size: 1842 1455 1230 627 549 518 369 325 217 BanII G_rGCy'C Cuts at: 1391 3576 4451 1391 Size: 2185 875 4072 Fragments arranged by size: 4072 2185 875 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 4411 928 Size: 971 1474 1038 3649 Fragments arranged by size: 3649 1474 1038 971 BbvCI CC'TCA_GC Cuts at: 3542 3542 Size: 7132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5323 5357 1903 Size: 34 3386 34 3678 Fragments arranged by size: 3678 3386 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5024 6234 5024 Size: 1210 5922 BclI T'GATC_A Cuts at: 1548 3088 3916 1548 Size: 1540 828 4764 Fragments arranged by size: 4764 1540 828 BfrBI ATG'CAT Cuts at: 3239 3420 3239 Size: 181 6951 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5743 136 Size: 107 122 71 631 605 4071 1525 Fragments arranged by size: 4071 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2085 7127 2085 Size: 5042 2090 BlpI GC'TnA_GC Cuts at: 4549 4549 Size: 7132 Bme1580I G_kGCm'C Cuts at: 1790 2140 2159 5193 6349 1790 Size: 350 19 3034 1156 2573 Fragments arranged by size: 3034 2573 1156 350 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7132 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4863 5821 442 Size: 416 53 83 399 24 3446 958 1753 Fragments arranged by size: 3446 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 3609 4245 5792 1239 Size: 1676 694 636 1547 2579 Fragments arranged by size: 2579 1676 1547 694 636 Bpu10I CC'TnA_GC Cuts at: 3542 3542 Size: 7132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5257 6122 6420 5257 Size: 865 298 5969 Fragments arranged by size: 5969 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5795 882 Size: 617 4296 2219 Fragments arranged by size: 4296 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4923 493 Size: 711 3719 2702 Fragments arranged by size: 3719 2702 711 BsaBI GATnn'nnATC Cuts at: 7126 7126 Size: 7132 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 4939 5321 5956 Size: 53 83 186 936 825 2581 382 635 Cuts at: 5956 275 Size: 1451 Fragments arranged by size: 2581 1451 936 825 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5565 5963 6237 6384 6795 5565 Size: 398 274 147 411 5902 Fragments arranged by size: 5902 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436 Size: 30 165 30 1468 30 45 30 460 Cuts at: 3436 3466 4131 4161 1178 Size: 30 665 30 4149 Fragments arranged by size: 4149 1468 665 460 165 45 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2970 3875 1186 Size: 1204 580 905 4443 Fragments arranged by size: 4443 1204 905 580 BseYI C'CCAG_C Cuts at: 2281 6335 6831 2281 Size: 4054 496 2582 Fragments arranged by size: 4054 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6559 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4582 5343 6371 1540 Size: 275 186 2581 761 1028 2301 Fragments arranged by size: 2581 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2632 3576 5193 5278 6349 2632 Size: 944 1617 85 1071 3415 Fragments arranged by size: 3415 1617 1071 944 85 BsmI GAATG_Cn' Cuts at: 2497 4643 4736 2497 Size: 2146 93 4893 Fragments arranged by size: 4893 2146 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2793 2963 2793 Size: 170 6962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2159 2632 3576 4451 5193 5278 Size: 399 350 19 473 944 875 742 85 Cuts at: 5278 6349 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 944 875 742 473 399 350 85 19 BspEI T'CCGG_A Cuts at: 6795 6795 Size: 7132 BspHI T'CATG_A Cuts at: 3846 5021 6751 3846 Size: 1175 1730 4227 Fragments arranged by size: 4227 1730 1175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4487 844 Size: 3643 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5019 1394 Size: 429 3196 3507 Fragments arranged by size: 3507 3196 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5627 5801 65 Size: 1183 4379 174 1396 Fragments arranged by size: 4379 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5776 5963 1700 Size: 110 3966 187 2869 Fragments arranged by size: 3966 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7132 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7132 BssSI C'ACGA_G Cuts at: 2853 4170 5192 6204 2853 Size: 1317 1022 1012 3781 Fragments arranged by size: 3781 1317 1022 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7132 BstBI TT'CG_AA Cuts at: 5936 5936 Size: 7132 BstEII G'GTnAC_C Cuts at: 1184 4273 4282 1184 Size: 3089 9 4034 Fragments arranged by size: 4034 3089 9 BstXI CCAn_nnnn'nTGG Cuts at: 2309 2309 Size: 7132 BstYI r'GATC_y Cuts at: 1972 2085 2393 3160 4397 4462 4829 5214 5231 Size: 113 308 767 1237 65 367 385 17 Cuts at: 5231 6683 6769 6781 7127 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1237 767 385 367 346 308 113 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2641 4096 513 Size: 552 1576 1455 3549 Fragments arranged by size: 3549 1576 1455 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6393 Fragments arranged by size: 6393 683 56 BtsI GCAGTG_nn' Cuts at: 4648 5442 5462 4648 Size: 794 20 6318 Fragments arranged by size: 6318 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7097 DraI TTT'AAA Cuts at: 3759 4436 3759 Size: 677 6455 DraIII CAC_nnn'GTG Cuts at: 2858 2858 Size: 7132 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6139 809 Size: 507 4823 1802 Fragments arranged by size: 4823 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4579 5468 7097 Size: 54 1425 50 275 18 2749 889 1629 Cuts at: 7097 8 Size: 43 Fragments arranged by size: 2749 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4579 1537 Size: 275 2767 4090 Fragments arranged by size: 4090 2767 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2791 3684 4263 5062 1353 Size: 1438 893 579 799 3423 Fragments arranged by size: 3423 1438 893 799 579 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5719 6093 6239 387 Size: 1064 138 4130 374 146 1280 Fragments arranged by size: 4130 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3574 3574 Size: 7132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2915 3609 4107 4245 4271 4331 Size: 393 526 757 694 498 138 26 60 Cuts at: 4331 5195 5792 6579 6847 1239 Size: 864 597 787 268 1524 Fragments arranged by size: 1524 864 787 757 694 597 526 498 393 268 138 60 26 EcoO109I rG'GnC_Cy Cuts at: 2788 3094 4193 2788 Size: 306 1099 5727 Fragments arranged by size: 5727 1099 306 EcoRI G'AATT_C Cuts at: 4052 4441 4819 4052 Size: 389 378 6365 Fragments arranged by size: 6365 389 378 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 7132 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2413 2759 3338 2188 Size: 225 346 579 5982 Fragments arranged by size: 5982 579 346 225 FspI TGC'GCA Cuts at: 5638 5638 Size: 7132 HaeII r_GCGC'y Cuts at: 1678 2350 3274 3529 6279 1678 Size: 672 924 255 2750 2531 Fragments arranged by size: 2750 2531 924 672 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946 Size: 32 135 32 505 32 623 32 182 Cuts at: 2946 2978 5773 5805 5847 5879 1373 Size: 32 2795 32 42 32 2626 Fragments arranged by size: 2795 2626 623 505 182 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 2529 4476 4722 669 Size: 988 281 115 476 1947 246 3079 Fragments arranged by size: 3079 1947 988 476 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 7132 HpaI GTT'AAC Cuts at: 2053 4722 2053 Size: 2669 4463 KpnI G_GTAC'C Cuts at: 2709 4457 2709 Size: 1748 5384 MfeI C'AATT_G Cuts at: 2988 4731 2988 Size: 1743 5389 MluI A'CGCG_T Cuts at: 7008 7008 Size: 7132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3889 6246 6430 1305 Size: 2584 2357 184 2007 Fragments arranged by size: 2584 2357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5310 Fragments arranged by size: 5310 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2307 4361 5090 5449 5608 518 Size: 648 1141 2054 729 359 159 2042 Fragments arranged by size: 2054 2042 1141 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2762 3398 4548 5225 6373 6618 1606 Size: 1156 636 1150 677 1148 245 2120 Fragments arranged by size: 2120 1156 1150 1148 677 636 245 NcoI C'CATG_G Cuts at: 513 1065 2641 4096 513 Size: 552 1576 1455 3549 Fragments arranged by size: 3549 1576 1455 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7132 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7132 NotI GC'GGCC_GC Cuts at: 4579 4579 Size: 7132 NsiI A_TGCA'T Cuts at: 3241 3422 3241 Size: 181 6951 NspI r_CATG'y Cuts at: 1132 1229 4490 6035 1132 Size: 97 3261 1545 2229 Fragments arranged by size: 3261 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7132 PciI A'CATG_T Cuts at: 6031 6031 Size: 7132 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7132 PmeI GTTT'AAAC Cuts at: 4436 4436 Size: 7132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5201 5233 6714 6746 5201 Size: 32 1481 32 5587 Fragments arranged by size: 5587 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 4193 3094 Size: 1099 6033 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7132 PsiI TTA'TAA Cuts at: 4702 4702 Size: 7132 PstI C_TGCA'G Cuts at: 830 3294 3562 4484 830 Size: 2464 268 922 3478 Fragments arranged by size: 3478 2464 922 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7132 PvuII CAG'CTG Cuts at: 2762 3398 2762 Size: 636 6496 SacI G_AGCT'C Cuts at: 3576 3576 Size: 7132 SalI G'TCGA_C Cuts at: 1936 4474 1936 Size: 2538 4594 SapI GCTCTTCn'nnn_ Cuts at: 3684 3684 Size: 7132 SbfI CC_TGCA'GG Cuts at: 4484 4484 Size: 7132 ScaI AGT'ACT Cuts at: 5380 7092 5380 Size: 1712 5420 SexAI A'CCwGG_T Cuts at: 3739 3739 Size: 7132 SfcI C'TryA_G Cuts at: 826 1045 3290 3558 4009 4480 5615 6296 6487 Size: 219 2245 268 451 471 1135 681 191 Cuts at: 6487 826 Size: 1471 Fragments arranged by size: 2245 1471 1135 681 471 451 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7132 SmaI CCC'GGG Cuts at: 1872 4459 1872 Size: 2587 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5236 6137 6399 820 Size: 197 926 3293 901 262 1553 Fragments arranged by size: 3293 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4923 493 Size: 4430 2702 SpeI A'CTAG_T Cuts at: 152 3638 152 Size: 3486 3646 SphI G_CATG'C Cuts at: 1132 4490 1132 Size: 3358 3774 SspI AAT'ATT Cuts at: 5 52 5056 5 Size: 47 5004 2081 Fragments arranged by size: 5004 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7132 StyI C'CwwG_G Cuts at: 513 1065 2119 2532 2641 2791 4096 4278 4571 Size: 552 1054 413 109 150 1305 182 293 Cuts at: 4571 513 Size: 3074 Fragments arranged by size: 3074 1305 1054 552 413 293 182 150 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5159 5176 5329 5514 5959 325 Size: 1255 3579 17 153 185 445 1498 Fragments arranged by size: 3579 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5378 7090 96 Size: 274 80 33 51 1443 3401 1712 138 Fragments arranged by size: 3401 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2871 2876 2903 3436 3468 4131 4163 7092 Size: 27 5 27 533 32 663 32 2929 Cuts at: 7092 7124 2844 Size: 32 2852 Fragments arranged by size: 2929 2852 663 533 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7132 XbaI T'CTAG_A Cuts at: 4400 4468 4400 Size: 68 7064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2603 2603 Size: 7132 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7132 XmaI C'CCGG_G Cuts at: 1870 4457 1870 Size: 2587 4545 XmnI GAAnn'nnTTC Cuts at: 2171 5261 2171 Size: 3090 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4940 276 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI