################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BbvCI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraIII EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 1279 CGCG CG^CG AflIII 524 ACATGT A^C[GA][CT]GT AhdI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1690 CCTCAGG CC^T[ACGT]AGG Aor51HI 27 AGCGCT AGC^GCT ApaI 1548 GGGCCC GGGCC^C AspEI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 436 GGCGCC GGCGC^C BbrPI 774 CACGTG CAC^GTG BbsI 958 GAAGAC GAAGAC(2/6) BciVI 1736 GTATCC GTATCC(6/5) BclI 2090 TGATCA T^GATCA BfaI 1267 CTAG C^TAG BglI 928 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 958 GAAGAC GAAGAC(2/6) BsiEI 17 CGGCCG CG[GA][CT]^CG BsmI 1600 GAATGC GAATGC(1/-1) BspLU11I 524 ACATGT A^CATGT BsrBI 1971 GAGCGG CCGCTC(-3/-3) BsrDI 270 CATTGC GCAATG(2/0) BstUI 1279 CGCG CG^CG Bsu36I 1690 CCTCAGG CC^T[ACGT]AGG DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 669 CCGTGG C^C[GA][CT]GG EagI 17 CGGCCG C^GGCCG Eam1105I 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2018 CTCTTC CTCTTC(1/4) EclXI 17 CGGCCG C^GGCCG Eco47III 27 AGCGCT AGC^GCT Eco52I 17 CGGCCG C^GGCCG Eco81I 1690 CCTCAGG CC^T[ACGT]AGG EcoRI 293 GAATTC G^AATTC FbaI 2090 TGATCA T^GATCA KasI 436 GGCGCC G^GCGCC Ksp632I 2018 CTCTTC CTCTTC(1/4) MaeI 1267 CTAG C^TAG MvnI 1279 CGCG CG^CG NaeI 1837 GCCGGC GCC^GGC NarI 436 GGCGCC GG^CGCC NgoMIV 1837 GCCGGC G^CCGGC NspI 524 ACATGT [GA]CATG^[CT] PflMI 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 774 CACGTG CAC^GTG PmlI 774 CACGTG CAC^GTG PpuMI 1444 GGGACCT [GA]G^G[AT]CC[CT] SfoI 436 GGCGCC GGC^GCC SmaI 728 CCCGGG CCC^GGG SmlI 1357 CTCAAG C^T[CT][GA]AG SspI 1063 AATATT AAT^ATT TaqI 529 TCGA T^CGA TfiI 1218 GATTC G^A[AT]TC TthHB8I 529 TCGA T^CGA Van91I 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 728 CCCGGG C^CCGGG XmaI 728 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 654 GTGCTC G[AT]GC[AT]^C 1527 GTGCTC BamHI 1161 GGATCC G^GATCC 2009 GGATCC BanI 436 GGCGCC G^G[CT][GA]CC 862 GGTGCC BsaAI 774 CACGTG [CT]AC^GT[GA] 819 CACGTA BsaWI 388 ACCGGA [AT]^CCGG[AT] 1450 TCCGGT BsiHKAI 654 GTGCTC G[AT]GC[AT]^C 1527 GTGCTC BsmBI 1569 CGTCTC CGTCTC(1/5) 764 GAGACG BsrFI 1116 GCCGGT [GA]^CCGG[CT] 1837 GCCGGC BssSI 99 CACGAG CACGAG(-5/-1) 915 CTCGTG BstAPI 1595 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1664 GCATACAGTGC BstYI 1161 GGATCC [GA]^GATC[CT] 2009 GGATCC Cfr10I 1116 GCCGGT [GA]^CCGG[CT] 1837 GCCGGC DraI 1033 TTTAAA TTT^AAA 1921 TTTAAA EaeI 17 CGGCCG [CT]^GGCC[GA] 1839 CGGCCA EcoNI 1620 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1686 CCTGCCTCAGG FauI 492 GCGGG CCCGC(4/6) 1973 GCGGG MflI 1161 GGATCC [GA]^GATC[CT] 2009 GGATCC PstI 310 CTGCAG CTGCA^G 710 CTGCAG SfcI 310 CTGCAG C^T[GA][CT]AG 710 CTGCAG XhoII 1161 GGATCC [GA]^GATC[CT] 2009 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I XcmI