(Linear) (Six-base) MAPSORT of: KIEE2774.seq Check: 5221 from: 1 to: 2115 KIEE2774.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 796 877 1717 2115 Size: 796 81 840 398 Fragments arranged by size: 840 796 398 81 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 2115 Size: 415 1700 AfeI AGC'GCT Cuts at: 0 29 2115 Size: 29 2086 AflIII A'CryG_T Cuts at: 0 524 2115 Size: 524 1591 AhdI GACnn_n'nnGTC Cuts at: 0 1451 2115 Size: 1451 664 AleI CACnn'nnGTG Cuts at: 0 871 2115 Size: 871 1244 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1321 1353 2115 Size: 1321 32 762 Fragments arranged by size: 1321 762 32 AlwNI CAG_nnn'CTG Cuts at: 0 627 689 1173 2115 Size: 627 62 484 942 Fragments arranged by size: 942 627 484 62 ApaI G_GGCC'C Cuts at: 0 1552 2115 Size: 1552 563 ApoI r'AATT_y Cuts at: 0 189 293 342 414 757 1030 1401 2115 Size: 189 104 49 72 343 273 371 714 Fragments arranged by size: 714 371 343 273 189 104 72 49 AvaI C'yCGr_G Cuts at: 0 728 841 1534 2115 Size: 728 113 693 581 Fragments arranged by size: 728 693 581 113 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1719 1752 2115 Size: 1719 33 363 Fragments arranged by size: 1719 363 33 BamHI G'GATC_C Cuts at: 0 1161 2009 2115 Size: 1161 848 106 Fragments arranged by size: 1161 848 106 BanI G'GyrC_C Cuts at: 0 436 862 2115 Size: 436 426 1253 Fragments arranged by size: 1253 436 426 BanII G_rGCy'C Cuts at: 0 72 1552 1559 1981 2115 Size: 72 1480 7 422 134 Fragments arranged by size: 1480 422 134 72 7 BbeI G_GCGC'C Cuts at: 0 440 2115 Size: 440 1675 BbsI GAAGACnn'nnnn_ Cuts at: 0 965 2115 Size: 965 1150 BciVI GTATCCnnnnn_n' Cuts at: 0 1747 2115 Size: 1747 368 BclI T'GATC_A Cuts at: 0 2090 2115 Size: 2090 25 BglI GCCn_nnn'nGGC Cuts at: 0 934 2115 Size: 934 1181 Bme1580I G_kGCm'C Cuts at: 0 127 648 1552 1749 2115 Size: 127 521 904 197 366 Fragments arranged by size: 904 521 366 197 127 BmrI ACTGGGnnnn_n' Cuts at: 0 677 2115 Size: 677 1438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 86 2115 Size: 86 2029 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 136 1645 2115 Size: 94 42 1509 470 Fragments arranged by size: 1509 470 94 42 Bpu10I CC'TnA_GC Cuts at: 0 354 1988 2001 2115 Size: 354 1634 13 114 Fragments arranged by size: 1634 354 114 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1342 2115 Size: 1342 773 BsaAI yAC'GTr Cuts at: 0 776 821 2115 Size: 776 45 1294 Fragments arranged by size: 1294 776 45 BsaHI Gr'CG_yC Cuts at: 0 437 965 1835 2115 Size: 437 528 870 280 Fragments arranged by size: 870 528 437 280 BsaWI w'CCGG_w Cuts at: 0 388 1450 2115 Size: 388 1062 665 Fragments arranged by size: 1062 665 388 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1030 1060 1072 1102 1134 1164 2115 Size: 1030 30 12 30 32 30 951 Fragments arranged by size: 1030 951 32 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 625 1142 1145 2094 2115 Size: 625 517 3 949 21 Fragments arranged by size: 949 625 517 21 3 BseYI C'CCAG_C Cuts at: 0 596 647 1544 1980 2040 2115 Size: 596 51 897 436 60 75 Fragments arranged by size: 897 596 436 75 60 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 623 702 1068 1695 1854 2115 Size: 229 394 79 366 627 159 261 Fragments arranged by size: 627 394 366 261 229 159 79 BsiEI CG_ry'CG Cuts at: 0 20 2115 Size: 20 2095 BsiHKAI G_wGCw'C Cuts at: 0 658 1531 2115 Size: 658 873 584 Fragments arranged by size: 873 658 584 BsmI GAATG_Cn' Cuts at: 0 1606 2115 Size: 1606 509 BsmBI CGTCTCn'nnnn_ Cuts at: 0 758 1575 2115 Size: 758 817 540 Fragments arranged by size: 817 758 540 Bsp1286I G_dGCh'C Cuts at: 0 72 127 648 658 1531 1552 1559 1749 Size: 72 55 521 10 873 21 7 190 Cuts at: 1749 1981 2115 Size: 232 134 Fragments arranged by size: 873 521 232 190 134 72 55 21 10 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 317 796 877 1717 1789 2115 Size: 317 479 81 840 72 326 Fragments arranged by size: 840 479 326 317 81 72 BsrBI CCG'CTC Cuts at: 0 1973 2115 Size: 1973 142 BsrDI GCAATG_nn' Cuts at: 0 269 2115 Size: 269 1846 BsrFI r'CCGG_y Cuts at: 0 1116 1837 2115 Size: 1116 721 278 Fragments arranged by size: 1116 721 278 BssSI C'ACGA_G Cuts at: 0 99 915 2115 Size: 99 816 1200 Fragments arranged by size: 1200 816 99 BstAPI GCAn_nnn'nTGC Cuts at: 0 1601 1670 2115 Size: 1601 69 445 Fragments arranged by size: 1601 445 69 BstYI r'GATC_y Cuts at: 0 1161 2009 2115 Size: 1161 848 106 Fragments arranged by size: 1161 848 106 Bsu36I CC'TnA_GG Cuts at: 0 1691 2115 Size: 1691 424 BtgI C'CryG_G Cuts at: 0 669 2115 Size: 669 1446 BtsI GCAGTG_nn' Cuts at: 0 513 658 1767 2115 Size: 513 145 1109 348 Fragments arranged by size: 1109 513 348 145 DraI TTT'AAA Cuts at: 0 1035 1923 2115 Size: 1035 888 192 Fragments arranged by size: 1035 888 192 DrdI GACnn_nn'nnGTC Cuts at: 0 745 2115 Size: 745 1370 EaeI y'GGCC_r Cuts at: 0 17 1839 2115 Size: 17 1822 276 Fragments arranged by size: 1822 276 17 EagI C'GGCC_G Cuts at: 0 17 2115 Size: 17 2098 EarI CTCTTCn'nnn_ Cuts at: 0 2024 2115 Size: 2024 91 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 136 415 1645 2115 Size: 94 42 279 1230 470 Fragments arranged by size: 1230 470 279 94 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 1624 1690 2115 Size: 1624 66 425 Fragments arranged by size: 1624 425 66 EcoO109I rG'GnC_Cy Cuts at: 0 724 991 1445 1548 2115 Size: 724 267 454 103 567 Fragments arranged by size: 724 567 454 267 103 EcoRI G'AATT_C Cuts at: 0 293 2115 Size: 293 1822 HaeII r_GCGC'y Cuts at: 0 31 40 440 666 2115 Size: 31 9 400 226 1449 Fragments arranged by size: 1449 400 226 31 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 898 930 1030 1062 1437 1469 1555 1587 Size: 898 32 100 32 375 32 86 32 Cuts at: 1587 2001 2033 2115 Size: 414 32 82 Fragments arranged by size: 898 414 375 100 86 82 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 436 2115 Size: 436 1679 MslI CAynn'nnrTG Cuts at: 0 529 871 2066 2115 Size: 529 342 1195 49 Fragments arranged by size: 1195 529 342 49 MspA1I CmG'CkG Cuts at: 0 557 1493 1984 2040 2115 Size: 557 936 491 56 75 Fragments arranged by size: 936 557 491 75 56 NaeI GCC'GGC Cuts at: 0 1839 2115 Size: 1839 276 NarI GG'CG_CC Cuts at: 0 437 2115 Size: 437 1678 NgoMIV G'CCGG_C Cuts at: 0 1837 2115 Size: 1837 278 NspI r_CATG'y Cuts at: 0 528 2115 Size: 528 1587 PciI A'CATG_T Cuts at: 0 524 2115 Size: 524 1591 PflMI CCAn_nnn'nTGG Cuts at: 0 252 2115 Size: 252 1863 PfoI T'CCnGG_A Cuts at: 0 10 245 2115 Size: 10 235 1870 Fragments arranged by size: 1870 235 10 PmlI CAC'GTG Cuts at: 0 776 2115 Size: 776 1339 PpuMI rG'GwC_Cy Cuts at: 0 1445 2115 Size: 1445 670 PsiI TTA'TAA Cuts at: 0 1471 2115 Size: 1471 644 PspOMI G'GGCC_C Cuts at: 0 1548 2115 Size: 1548 567 PstI C_TGCA'G Cuts at: 0 314 714 2115 Size: 314 400 1401 Fragments arranged by size: 1401 400 314 PvuII CAG'CTG Cuts at: 0 557 1493 1984 2040 2115 Size: 557 936 491 56 75 Fragments arranged by size: 936 557 491 75 56 SfcI C'TryA_G Cuts at: 0 310 710 2115 Size: 310 400 1405 Fragments arranged by size: 1405 400 310 SfoI GGC'GCC Cuts at: 0 438 2115 Size: 438 1677 SmaI CCC'GGG Cuts at: 0 730 2115 Size: 730 1385 SmlI C'TyrA_G Cuts at: 0 1357 2115 Size: 1357 758 SspI AAT'ATT Cuts at: 0 1065 2115 Size: 1065 1050 StyI C'CwwG_G Cuts at: 0 93 183 1340 1559 1636 2115 Size: 93 90 1157 219 77 479 Fragments arranged by size: 1157 479 219 93 90 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1691 2115 Size: 1691 424 TatI w'GTAC_w Cuts at: 0 332 2115 Size: 332 1783 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1134 1166 2115 Size: 1134 32 949 Fragments arranged by size: 1134 949 32 Tth111I GACn'n_nGTC Cuts at: 0 499 964 1567 2115 Size: 499 465 603 548 Fragments arranged by size: 603 548 499 465 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 249 253 825 2115 Size: 249 4 572 1290 Fragments arranged by size: 1290 572 249 4 XmaI C'CCGG_G Cuts at: 0 728 2115 Size: 728 1387 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI PvuII SfcI SfoI SmaI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AlfI ApaLI AscI AseI AsiSI AvrII BbvCI BcgI BfrBI BglII BlpI BmgBI BmtI BsaI BsaBI BsiWI BspEI BspHI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII EciI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI XbaI XhoI XmnI ZraI