(Linear) (Six-base) MAPSORT of: KIEE2776.seq Check: 2204 from: 1 to: 1215 KIEE2776.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 796 877 1215 Size: 796 81 338 Fragments arranged by size: 796 338 81 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1215 Size: 415 800 AfeI AGC'GCT Cuts at: 0 29 1215 Size: 29 1186 AflIII A'CryG_T Cuts at: 0 524 1215 Size: 524 691 AleI CACnn'nnGTG Cuts at: 0 871 1215 Size: 871 344 AlwNI CAG_nnn'CTG Cuts at: 0 627 689 1173 1215 Size: 627 62 484 42 Fragments arranged by size: 627 484 62 42 ApoI r'AATT_y Cuts at: 0 189 293 342 414 757 1030 1215 Size: 189 104 49 72 343 273 185 Fragments arranged by size: 343 273 189 185 104 72 49 AvaI C'yCGr_G Cuts at: 0 728 841 1215 Size: 728 113 374 Fragments arranged by size: 728 374 113 BamHI G'GATC_C Cuts at: 0 1161 1215 Size: 1161 54 BanI G'GyrC_C Cuts at: 0 436 862 1215 Size: 436 426 353 Fragments arranged by size: 436 426 353 BanII G_rGCy'C Cuts at: 0 72 1215 Size: 72 1143 BbeI G_GCGC'C Cuts at: 0 440 1215 Size: 440 775 BbsI GAAGACnn'nnnn_ Cuts at: 0 965 1215 Size: 965 250 BglI GCCn_nnn'nGGC Cuts at: 0 934 1215 Size: 934 281 Bme1580I G_kGCm'C Cuts at: 0 127 648 1215 Size: 127 521 567 Fragments arranged by size: 567 521 127 BmrI ACTGGGnnnn_n' Cuts at: 0 677 1215 Size: 677 538 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 86 1215 Size: 86 1129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 136 1215 Size: 94 42 1079 Fragments arranged by size: 1079 94 42 Bpu10I CC'TnA_GC Cuts at: 0 354 1215 Size: 354 861 BsaAI yAC'GTr Cuts at: 0 776 821 1215 Size: 776 45 394 Fragments arranged by size: 776 394 45 BsaHI Gr'CG_yC Cuts at: 0 437 965 1215 Size: 437 528 250 Fragments arranged by size: 528 437 250 BsaWI w'CCGG_w Cuts at: 0 388 1215 Size: 388 827 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1030 1060 1072 1102 1134 1164 1215 Size: 1030 30 12 30 32 30 51 Fragments arranged by size: 1030 51 32 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 625 1142 1145 1215 Size: 625 517 3 70 Fragments arranged by size: 625 517 70 3 BseYI C'CCAG_C Cuts at: 0 596 647 1215 Size: 596 51 568 Fragments arranged by size: 596 568 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 623 702 1068 1215 Size: 229 394 79 366 147 Fragments arranged by size: 394 366 229 147 79 BsiEI CG_ry'CG Cuts at: 0 20 1215 Size: 20 1195 BsiHKAI G_wGCw'C Cuts at: 0 658 1215 Size: 658 557 BsmBI CGTCTCn'nnnn_ Cuts at: 0 758 1215 Size: 758 457 Bsp1286I G_dGCh'C Cuts at: 0 72 127 648 658 1215 Size: 72 55 521 10 557 Fragments arranged by size: 557 521 72 55 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 317 796 877 1215 Size: 317 479 81 338 Fragments arranged by size: 479 338 317 81 BsrDI GCAATG_nn' Cuts at: 0 269 1215 Size: 269 946 BsrFI r'CCGG_y Cuts at: 0 1116 1215 Size: 1116 99 BssSI C'ACGA_G Cuts at: 0 99 915 1215 Size: 99 816 300 Fragments arranged by size: 816 300 99 BstYI r'GATC_y Cuts at: 0 1161 1215 Size: 1161 54 BtgI C'CryG_G Cuts at: 0 669 1215 Size: 669 546 BtsI GCAGTG_nn' Cuts at: 0 513 658 1215 Size: 513 145 557 Fragments arranged by size: 557 513 145 DraI TTT'AAA Cuts at: 0 1035 1215 Size: 1035 180 DrdI GACnn_nn'nnGTC Cuts at: 0 745 1215 Size: 745 470 EaeI y'GGCC_r Cuts at: 0 17 1215 Size: 17 1198 EagI C'GGCC_G Cuts at: 0 17 1215 Size: 17 1198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 136 415 1215 Size: 94 42 279 800 Fragments arranged by size: 800 279 94 42 EcoO109I rG'GnC_Cy Cuts at: 0 724 991 1215 Size: 724 267 224 Fragments arranged by size: 724 267 224 EcoRI G'AATT_C Cuts at: 0 293 1215 Size: 293 922 HaeII r_GCGC'y Cuts at: 0 31 40 440 666 1215 Size: 31 9 400 226 549 Fragments arranged by size: 549 400 226 31 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 898 930 1030 1062 1215 Size: 898 32 100 32 153 Fragments arranged by size: 898 153 100 32 32 KasI G'GCGC_C Cuts at: 0 436 1215 Size: 436 779 MslI CAynn'nnrTG Cuts at: 0 529 871 1215 Size: 529 342 344 Fragments arranged by size: 529 344 342 MspA1I CmG'CkG Cuts at: 0 557 1215 Size: 557 658 NarI GG'CG_CC Cuts at: 0 437 1215 Size: 437 778 NspI r_CATG'y Cuts at: 0 528 1215 Size: 528 687 PciI A'CATG_T Cuts at: 0 524 1215 Size: 524 691 PflMI CCAn_nnn'nTGG Cuts at: 0 252 1215 Size: 252 963 PfoI T'CCnGG_A Cuts at: 0 10 245 1215 Size: 10 235 970 Fragments arranged by size: 970 235 10 PmlI CAC'GTG Cuts at: 0 776 1215 Size: 776 439 PstI C_TGCA'G Cuts at: 0 314 714 1215 Size: 314 400 501 Fragments arranged by size: 501 400 314 PvuII CAG'CTG Cuts at: 0 557 1215 Size: 557 658 SfcI C'TryA_G Cuts at: 0 310 710 1215 Size: 310 400 505 Fragments arranged by size: 505 400 310 SfoI GGC'GCC Cuts at: 0 438 1215 Size: 438 777 SmaI CCC'GGG Cuts at: 0 730 1215 Size: 730 485 SspI AAT'ATT Cuts at: 0 1065 1215 Size: 1065 150 StyI C'CwwG_G Cuts at: 0 93 183 1215 Size: 93 90 1032 Fragments arranged by size: 1032 93 90 TatI w'GTAC_w Cuts at: 0 332 1215 Size: 332 883 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1134 1166 1215 Size: 1134 32 49 Fragments arranged by size: 1134 49 32 Tth111I GACn'n_nGTC Cuts at: 0 499 964 1215 Size: 499 465 251 Fragments arranged by size: 499 465 251 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 249 253 825 1215 Size: 249 4 572 390 Fragments arranged by size: 572 390 249 4 XmaI C'CCGG_G Cuts at: 0 728 1215 Size: 728 487 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstYI BtgI BtsI DraI DrdI EaeI EagI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI MslI MspA1I NarI NspI PciI PflMI PfoI PmlI PstI PvuII SfcI SfoI SmaI SspI StyI TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EarI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII XbaI XhoI XmnI ZraI