(Linear) (Six-base) MAPSORT of: KIEE2748.seq Check: 433 from: 1 to: 7395 KIEE2748.orf With 182 enzymes: SgfI * June 20, 2019 10:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 6069 7395 Size: 6069 1326 AccI GT'mk_AC Cuts at: 0 210 429 5246 7026 7395 Size: 210 219 4817 1780 369 Fragments arranged by size: 4817 1780 369 219 210 AclI AA'CG_TT Cuts at: 0 3051 7395 Size: 3051 4344 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 683 956 1176 1345 1769 1958 3301 3957 Size: 683 273 220 169 424 189 1343 656 Cuts at: 3957 4240 4741 4820 4861 5336 5689 5884 6785 Size: 283 501 79 41 475 353 195 901 Cuts at: 6785 7395 Size: 610 Fragments arranged by size: 1343 901 683 656 610 501 475 424 353 283 273 220 195 189 169 79 41 AflII C'TTAA_G Cuts at: 0 2520 4747 7395 Size: 2520 2227 2648 Fragments arranged by size: 2648 2520 2227 AflIII A'CryG_T Cuts at: 0 4101 4903 7395 Size: 4101 802 2492 Fragments arranged by size: 4101 2492 802 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2040 3131 4747 7395 Size: 2040 1091 1616 2648 Fragments arranged by size: 2648 2040 1616 1091 AlwNI CAG_nnn'CTG Cuts at: 0 1527 1604 2379 3648 4764 5617 7188 7395 Size: 1527 77 775 1269 1116 853 1571 207 Fragments arranged by size: 1571 1527 1269 1116 853 775 207 77 ApaI G_GGCC'C Cuts at: 0 5449 6809 7395 Size: 5449 1360 586 Fragments arranged by size: 5449 1360 586 ApaLI G'TGCA_C Cuts at: 0 6239 7395 Size: 6239 1156 ApoI r'AATT_y Cuts at: 0 78 2169 2605 3111 4361 4472 4711 5064 Size: 78 2091 436 506 1250 111 239 353 Cuts at: 5064 5732 6907 7039 7395 Size: 668 1175 132 356 Fragments arranged by size: 2091 1250 1175 668 506 436 356 353 239 132 111 78 AseI AT'TA_AT Cuts at: 0 4694 5396 7395 Size: 4694 702 1999 Fragments arranged by size: 4694 1999 702 AvaI C'yCGr_G Cuts at: 0 3330 7395 Size: 3330 4065 BamHI G'GATC_C Cuts at: 0 5208 6348 7395 Size: 5208 1140 1047 Fragments arranged by size: 5208 1140 1047 BanI G'GyrC_C Cuts at: 0 6636 7395 Size: 6636 759 BanII G_rGCy'C Cuts at: 0 5449 6809 7395 Size: 5449 1360 586 Fragments arranged by size: 5449 1360 586 BbsI GAAGACnn'nnnn_ Cuts at: 0 556 864 1715 2005 2605 5517 5797 7112 Size: 556 308 851 290 600 2912 280 1315 Cuts at: 7112 7209 7395 Size: 97 186 Fragments arranged by size: 2912 1315 851 600 556 308 290 280 186 97 BbvCI CC'TCA_GC Cuts at: 0 2294 2660 7395 Size: 2294 366 4735 Fragments arranged by size: 4735 2294 366 BclI T'GATC_A Cuts at: 0 797 5300 7395 Size: 797 4503 2095 Fragments arranged by size: 4503 2095 797 BfrBI ATG'CAT Cuts at: 0 868 4231 6109 6207 7395 Size: 868 3363 1878 98 1188 Fragments arranged by size: 3363 1878 1188 868 98 BglII A'GATC_T Cuts at: 0 303 2823 7395 Size: 303 2520 4572 Fragments arranged by size: 4572 2520 303 BlpI GC'TnA_GC Cuts at: 0 4518 4892 6280 7395 Size: 4518 374 1388 1115 Fragments arranged by size: 4518 1388 1115 374 Bme1580I G_kGCm'C Cuts at: 0 552 5449 5744 6221 6243 6809 7145 7395 Size: 552 4897 295 477 22 566 336 250 Fragments arranged by size: 4897 566 552 477 336 295 250 22 BmrI ACTGGGnnnn_n' Cuts at: 0 1515 2275 2492 6347 7395 Size: 1515 760 217 3855 1048 Fragments arranged by size: 3855 1515 1048 760 217 BmtI G_CTAG'C Cuts at: 0 1652 7395 Size: 1652 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 75 1394 1546 1676 2356 3681 4037 Size: 37 38 1319 152 130 680 1325 356 Cuts at: 4037 4102 5798 6844 7327 7395 Size: 65 1696 1046 483 68 Fragments arranged by size: 1696 1325 1319 1046 680 483 356 152 130 68 65 38 37 Bpu10I CC'TnA_GC Cuts at: 0 287 832 1091 1618 2294 2660 7395 Size: 287 545 259 527 676 366 4735 Fragments arranged by size: 4735 676 545 527 366 287 259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 696 2256 3384 5439 6638 7395 Size: 696 1560 1128 2055 1199 757 Fragments arranged by size: 2055 1560 1199 1128 757 696 BsaI GGTCTCn'nnnn_ Cuts at: 0 5888 7395 Size: 5888 1507 BsaAI yAC'GTr Cuts at: 0 602 7053 7395 Size: 602 6451 342 Fragments arranged by size: 6451 602 342 BsaBI GATnn'nnATC Cuts at: 0 802 3287 3560 7395 Size: 802 2485 273 3835 Fragments arranged by size: 3835 2485 802 273 BsaHI Gr'CG_yC Cuts at: 0 5764 7395 Size: 5764 1631 BsaWI w'CCGG_w Cuts at: 0 789 4277 4340 7395 Size: 789 3488 63 3055 Fragments arranged by size: 3488 3055 789 63 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2354 2384 3224 3254 3780 3810 4091 4121 Size: 2354 30 840 30 526 30 281 30 Cuts at: 4121 6817 6847 7395 Size: 2696 30 548 Fragments arranged by size: 2696 2354 840 548 526 281 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 3100 3699 4229 7395 Size: 3100 599 530 3166 Fragments arranged by size: 3166 3100 599 530 BseYI C'CCAG_C Cuts at: 0 3352 5759 7395 Size: 3352 2407 1636 Fragments arranged by size: 3352 2407 1636 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 2374 2495 3570 4409 4946 5079 7146 Size: 724 1650 121 1075 839 537 133 2067 Cuts at: 7146 7395 Size: 249 Fragments arranged by size: 2067 1650 1075 839 724 537 249 133 121 BsiEI CG_ry'CG Cuts at: 0 790 7395 Size: 790 6605 BsiHKAI G_wGCw'C Cuts at: 0 2272 4109 4617 6243 7208 7395 Size: 2272 1837 508 1626 965 187 Fragments arranged by size: 2272 1837 1626 965 508 187 BsmI GAATG_Cn' Cuts at: 0 538 2391 3399 3621 7395 Size: 538 1853 1008 222 3774 Fragments arranged by size: 3774 1853 1008 538 222 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3568 7395 Size: 3568 3827 Bsp1286I G_dGCh'C Cuts at: 0 552 2272 4109 4617 5449 5744 6221 6243 Size: 552 1720 1837 508 832 295 477 22 Cuts at: 6243 6809 7145 7208 7395 Size: 566 336 63 187 Fragments arranged by size: 1837 1720 832 566 552 508 477 336 295 187 63 22 BspEI T'CCGG_A Cuts at: 0 4340 7395 Size: 4340 3055 BspHI T'CATG_A Cuts at: 0 1734 3744 5603 7395 Size: 1734 2010 1859 1792 Fragments arranged by size: 2010 1859 1792 1734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 4633 6069 7395 Size: 4633 1436 1326 Fragments arranged by size: 4633 1436 1326 BsrBI CCG'CTC Cuts at: 0 6177 7395 Size: 6177 1218 BsrDI GCAATG_nn' Cuts at: 0 370 998 1567 2024 2288 2986 3132 5486 Size: 370 628 569 457 264 698 146 2354 Cuts at: 5486 6071 6928 7395 Size: 585 857 467 Fragments arranged by size: 2354 857 698 628 585 569 467 457 370 264 146 BsrGI T'GTAC_A Cuts at: 0 723 3039 4352 6741 7395 Size: 723 2316 1313 2389 654 Fragments arranged by size: 2389 2316 1313 723 654 BssSI C'ACGA_G Cuts at: 0 2972 6452 7395 Size: 2972 3480 943 Fragments arranged by size: 3480 2972 943 BstAPI GCAn_nnn'nTGC Cuts at: 0 349 3105 5935 6247 7149 7188 7395 Size: 349 2756 2830 312 902 39 207 Fragments arranged by size: 2830 2756 902 349 312 207 39 BstBI TT'CG_AA Cuts at: 0 1286 7395 Size: 1286 6109 BstXI CCAn_nnnn'nTGG Cuts at: 0 5751 6012 7395 Size: 5751 261 1383 Fragments arranged by size: 5751 1383 261 BstYI r'GATC_y Cuts at: 0 303 803 1047 1170 1613 1933 2127 2823 Size: 303 500 244 123 443 320 194 696 Cuts at: 2823 3561 4057 5208 6348 7395 Size: 738 496 1151 1140 1047 Fragments arranged by size: 1151 1140 1047 738 696 500 496 443 320 303 244 194 123 BstZ17I GTA'TAC Cuts at: 0 7027 7395 Size: 7027 368 BtgI C'CryG_G Cuts at: 0 2474 3182 7395 Size: 2474 708 4213 Fragments arranged by size: 4213 2474 708 BtsI GCAGTG_nn' Cuts at: 0 669 1345 2029 3261 3558 4934 5280 5317 Size: 669 676 684 1232 297 1376 346 37 Cuts at: 5317 6467 6567 6773 7395 Size: 1150 100 206 622 Fragments arranged by size: 1376 1232 1150 684 676 669 622 346 297 206 100 37 DraI TTT'AAA Cuts at: 0 620 5645 7044 7395 Size: 620 5025 1399 351 Fragments arranged by size: 5025 1399 620 351 DraIII CAC_nnn'GTG Cuts at: 0 5275 7395 Size: 5275 2120 DrdI GACnn_nn'nnGTC Cuts at: 0 3788 7395 Size: 3788 3607 EaeI y'GGCC_r Cuts at: 0 2471 4268 7395 Size: 2471 1797 3127 Fragments arranged by size: 3127 2471 1797 EarI CTCTTCn'nnn_ Cuts at: 0 5 341 757 1022 2263 2532 2668 3566 Size: 5 336 416 265 1241 269 136 898 Cuts at: 3566 3746 3756 4492 4796 4880 7346 7395 Size: 180 10 736 304 84 2466 49 Fragments arranged by size: 2466 1241 898 736 416 336 304 269 265 180 136 84 49 10 5 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 75 683 956 1176 1345 1394 1546 Size: 37 38 608 273 220 169 49 152 Cuts at: 1546 1676 1769 1958 2356 3301 3681 3957 4037 Size: 130 93 189 398 945 380 276 80 Cuts at: 4037 4102 4240 4741 4820 4861 5336 5689 5798 Size: 65 138 501 79 41 475 353 109 Cuts at: 5798 5884 6785 6844 7327 7395 Size: 86 901 59 483 68 Fragments arranged by size: 945 901 608 501 483 475 398 380 353 276 273 220 189 169 152 138 130 109 93 86 80 79 68 65 59 49 41 38 37 EcoNI CCTnn'n_nnAGG Cuts at: 0 1094 1621 2569 7395 Size: 1094 527 948 4826 Fragments arranged by size: 4826 1094 948 527 EcoO109I rG'GnC_Cy Cuts at: 0 907 1099 5446 6805 6806 7395 Size: 907 192 4347 1359 1 589 Fragments arranged by size: 4347 1359 907 589 192 1 EcoRI G'AATT_C Cuts at: 0 5732 7395 Size: 5732 1663 EcoRV GAT'ATC Cuts at: 0 113 7395 Size: 113 7282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 664 2146 3919 4600 5584 7395 Size: 664 1482 1773 681 984 1811 Fragments arranged by size: 1811 1773 1482 984 681 664 FspI TGC'GCA Cuts at: 0 7171 7395 Size: 7171 224 HaeII r_GCGC'y Cuts at: 0 250 7395 Size: 250 7145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 97 129 381 413 1542 1574 2549 2581 Size: 97 32 252 32 1129 32 975 32 Cuts at: 2581 3224 3256 4540 4572 5148 5180 6040 6072 Size: 643 32 1284 32 576 32 860 32 Cuts at: 6072 6254 6286 7395 Size: 182 32 1109 Fragments arranged by size: 1284 1129 1109 975 860 643 576 252 182 97 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2753 7395 Size: 2753 4642 HindIII A'AGCT_T Cuts at: 0 628 6329 7395 Size: 628 5701 1066 Fragments arranged by size: 5701 1066 628 HpaI GTT'AAC Cuts at: 0 2753 7395 Size: 2753 4642 MfeI C'AATT_G Cuts at: 0 59 1889 5930 5939 7395 Size: 59 1830 4041 9 1456 Fragments arranged by size: 4041 1830 1456 59 9 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 485 1148 1418 2067 5186 5236 7395 Size: 485 663 270 649 3119 50 2159 Fragments arranged by size: 3119 2159 663 649 485 270 50 MscI TGG'CCA Cuts at: 0 2473 4270 7395 Size: 2473 1797 3125 Fragments arranged by size: 3125 2473 1797 MslI CAynn'nnrTG Cuts at: 0 358 1039 3677 4318 4456 5455 5496 7395 Size: 358 681 2638 641 138 999 41 1899 Fragments arranged by size: 2638 1899 999 681 641 358 138 41 MspA1I CmG'CkG Cuts at: 0 1404 1879 6276 6876 7395 Size: 1404 475 4397 600 519 Fragments arranged by size: 4397 1404 600 519 475 NcoI C'CATG_G Cuts at: 0 2474 3182 7395 Size: 2474 708 4213 Fragments arranged by size: 4213 2474 708 NdeI CA'TA_TG Cuts at: 0 258 5297 7395 Size: 258 5039 2098 Fragments arranged by size: 5039 2098 258 NheI G'CTAG_C Cuts at: 0 1648 7395 Size: 1648 5747 NsiI A_TGCA'T Cuts at: 0 870 4233 6111 6209 7395 Size: 870 3363 1878 98 1186 Fragments arranged by size: 3363 1878 1186 870 98 NspI r_CATG'y Cuts at: 0 946 1354 2353 2620 2791 4105 4392 4455 Size: 946 408 999 267 171 1314 287 63 Cuts at: 4455 4891 4907 4917 4961 5703 7395 Size: 436 16 10 44 742 1692 Fragments arranged by size: 1692 1314 999 946 742 436 408 287 267 171 63 44 16 10 PciI A'CATG_T Cuts at: 0 4101 4903 7395 Size: 4101 802 2492 Fragments arranged by size: 4101 2492 802 PflMI CCAn_nnn'nTGG Cuts at: 0 98 993 5235 7395 Size: 98 895 4242 2160 Fragments arranged by size: 4242 2160 895 98 PfoI T'CCnGG_A Cuts at: 0 3078 3697 4053 4135 7395 Size: 3078 619 356 82 3260 Fragments arranged by size: 3260 3078 619 356 82 PpuMI rG'GwC_Cy Cuts at: 0 907 1099 7395 Size: 907 192 6296 Fragments arranged by size: 6296 907 192 PshAI GACnn'nnGTC Cuts at: 0 5539 7395 Size: 5539 1856 PsiI TTA'TAA Cuts at: 0 2740 7395 Size: 2740 4655 PspOMI G'GGCC_C Cuts at: 0 5445 6805 7395 Size: 5445 1360 590 Fragments arranged by size: 5445 1360 590 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3621 3653 7395 Size: 3621 32 3742 Fragments arranged by size: 3742 3621 32 PstI C_TGCA'G Cuts at: 0 3124 3663 5027 5553 7395 Size: 3124 539 1364 526 1842 Fragments arranged by size: 3124 1842 1364 539 526 PvuII CAG'CTG Cuts at: 0 1404 1879 6276 7395 Size: 1404 475 4397 1119 Fragments arranged by size: 4397 1404 1119 475 SapI GCTCTTCn'nnn_ Cuts at: 0 5 2263 7395 Size: 5 2258 5132 Fragments arranged by size: 5132 2258 5 ScaI AGT'ACT Cuts at: 0 481 696 7395 Size: 481 215 6699 Fragments arranged by size: 6699 481 215 SexAI A'CCwGG_T Cuts at: 0 225 423 2447 4652 7395 Size: 225 198 2024 2205 2743 Fragments arranged by size: 2743 2205 2024 225 198 SfcI C'TryA_G Cuts at: 0 430 543 2111 3120 3659 4187 5023 5549 Size: 430 113 1568 1009 539 528 836 526 Cuts at: 5549 6159 7395 Size: 610 1236 Fragments arranged by size: 1568 1236 1009 836 610 539 528 526 430 113 SmlI C'TyrA_G Cuts at: 0 711 2271 2520 3363 4747 5418 6653 7395 Size: 711 1560 249 843 1384 671 1235 742 Fragments arranged by size: 1560 1384 1235 843 742 711 671 249 SpeI A'CTAG_T Cuts at: 0 2436 4216 7395 Size: 2436 1780 3179 Fragments arranged by size: 3179 2436 1780 SphI G_CATG'C Cuts at: 0 4891 5703 7395 Size: 4891 812 1692 Fragments arranged by size: 4891 1692 812 SspI AAT'ATT Cuts at: 0 6201 7395 Size: 6201 1194 StuI AGG'CCT Cuts at: 0 5121 7395 Size: 5121 2274 StyI C'CwwG_G Cuts at: 0 2474 3182 3419 5537 5666 7395 Size: 2474 708 237 2118 129 1729 Fragments arranged by size: 2474 2118 1729 708 237 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1847 4985 7395 Size: 1847 3138 2410 Fragments arranged by size: 3138 2410 1847 TatI w'GTAC_w Cuts at: 0 479 694 717 723 3030 3039 3138 4352 Size: 479 215 23 6 2307 9 99 1214 Cuts at: 4352 5087 6302 6741 7395 Size: 735 1215 439 654 Fragments arranged by size: 2307 1215 1214 735 654 479 439 215 99 23 9 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2354 2386 3201 3233 7395 Size: 2354 32 815 32 4162 Fragments arranged by size: 4162 2354 815 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2463 7395 Size: 2463 4932 XbaI T'CTAG_A Cuts at: 0 1110 3778 4773 7395 Size: 1110 2668 995 2622 Fragments arranged by size: 2668 2622 1110 995 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2647 7395 Size: 2647 4748 XmnI GAAnn'nnTTC Cuts at: 0 1539 3314 4528 5585 6944 7395 Size: 1539 1775 1214 1057 1359 451 Fragments arranged by size: 1775 1539 1359 1214 1057 451 Enzymes that do cut: AarI AccI AclI AcuI AflII AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtsI DraI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SapI ScaI SexAI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AgeI AhdI AleI AloI AscI AsiSI AvrII BaeI BbeI BcgI BciVI BglI BmgBI BplI BsiWI BsrFI BssHII BstEII Bsu36I BtgZI ClaI CspCI EagI EciI EcoICRI FseI FspAI KasI KpnI MluI NaeI NarI NgoMIV NotI NruI PacI PasI PmeI PmlI PpiI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI XhoI XmaI ZraI