Restriction map for
pFN21AE9244
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Aor51HI ApaI
AscI Asp718I AspI AvaI AvrII
BanI BbeI BbvCI BcgI BciVI
BfrI BglI BlnI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DrdI DsaI EagI
EclXI Eco47III Eco52I EcoO65I FauI
FseI HaeII Hin1I HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NotI
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 3222 GTCGAC GT^[AC][GT]AC
AccII 4405 CGCG CG^CG
AhdI 1269 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2451 GTGCAC G^TGCAC
AocI 4334 CCTTAGG CC^T[ACGT]AGG
ApaLI 2451 GTGCAC G^TGCAC
AspEI 1269 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3764 TGCGCA TGC^GCA
BalI 3390 TGGCCA TGG^CCA
BbrPI 1201 CACGTG CAC^GTG
BcnI 1319 CCGGG CC^[GC]GG
BglII 1737 AGATCT A^GATCT
BsaWI 4672 TCCGGT [AT]^CCGG[AT]
BspMI 3408 ACCTGC ACCTGC(4/8)
BsrDI 2347 GCAATG GCAATG(2/0)
BsrFI 2936 ACCGGC [GA]^CCGG[CT]
BstBI 652 TTCGAA TT^CGAA
BstUI 4405 CGCG CG^CG
Bsu36I 4334 CCTTAGG CC^T[ACGT]AGG
CfoI 3765 GCGC GCG^C
Cfr10I 2936 ACCGGC [GA]^CCGG[CT]
DraI 4428 TTTAAA TTT^AAA
DraIII 1744 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1269 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4334 CCTTAGG CC^T[ACGT]AGG
EcoRI 4156 GAATTC G^AATTC
FspI 3764 TGCGCA TGC^GCA
HgaI 1680 GCGTC GACGC(5/10)
HhaI 3765 GCGC GCG^C
HinP1I 3765 GCGC G^CGC
HindIII 1011 AAGCTT A^AGCTT
MfeI 208 CAATTG C^AATTG
MluNI 3390 TGGCCA TGG^CCA
MscI 3390 TGGCCA TGG^CCA
MunI 208 CAATTG C^AATTG
MvnI 4405 CGCG CG^CG
NciI 1319 CCGGG CC^[GC]GG
NheI 3847 GCTAGC G^CTAGC
NruI 4404 TCGCGA TCG^CGA
NspV 652 TTCGAA TT^CGAA
PflMI 3601 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1201 CACGTG CAC^GTG
PmlI 1201 CACGTG CAC^GTG
PshAI 3061 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3222 GTCGAC G^TCGAC
SfuI 652 TTCGAA TT^CGAA
SmlI 2812 CTTGAG C^T[CT][GA]AG
StuI 657 AGGCCT AGG^CCT
Van91I 3601 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwNI 1021 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3458 CAGAAGCTG
BamHI 3101 GGATCC G^GATCC
4135 GGATCC
BanII 148 GAGCTC G[GA]GC[CT]^C
3358 GAGCTC
BclI 974 TGATCA T^GATCA
2952 TGATCA
BlpI 1339 GCTCAGC GC^T[ACGT]AGC
3427 GCTCAGC
Bpu1102I 1339 GCTCAGC GC^T[ACGT]AGC
3427 GCTCAGC
BsaBI 424 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3979 GATGTGAATC
BsaI 3398 GAGACC GGTCTC(1/5)
2676 GGTCTC
BsgI 2185 GTGCAG GTGCAG(16/14)
2208 GTGCAG
BsmI 3840 GAATGC GAATGC(1/-1)
4305 GCATTC
Bsp1407I 1581 TGTACA T^GTACA
4602 TGTACA
BsrGI 1581 TGTACA T^GTACA
4602 TGTACA
BstAPI 231 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2083 GCAAAGCATGC
CelII 1339 GCTCAGC GC^T[ACGT]AGC
3427 GCTCAGC
DraII 540 GGGTCCC [GA]G^G[ACGT]CC[CT]
4529 GGGACCT
EaeI 3353 TGGCCG [CT]^GGCC[GA]
3390 TGGCCA
EcoNI 361 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4228 CCTTGTGAAGG
EcoO109I 540 GGGTCCC [GA]G^G[ACGT]CC[CT]
4529 GGGACCT
EcoRV 800 GATATC GAT^ATC
2694 GATATC
EcoT22I 4386 ATGCAT ATGCA^T
4390 ATGCAT
FbaI 974 TGATCA T^GATCA
2952 TGATCA
MamI 424 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3979 GATGTGAATC
NsiI 4386 ATGCAT ATGCA^T
4390 ATGCAT
PpuMI 540 GGGTCCC [GA]G^G[AT]CC[CT]
4529 GGGACCT
SacI 148 GAGCTC GAGCT^C
3358 GAGCTC
SphI 2088 GCATGC GCATG^C
4388 GCATGC
SspBI 1581 TGTACA T^GTACA
4602 TGTACA
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI