(Linear) (Six-base) MAPSORT of: KIEE9244.seq Check: 189 from: 1 to: 4962 KIEE9244.orf With 182 enzymes: SgfI * October 3, 2019 17:18 .. AccI GT'mk_AC Cuts at: 0 3223 4962 Size: 3223 1739 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 356 383 706 1651 1943 2732 2785 3013 Size: 356 27 323 945 292 789 53 228 Cuts at: 3013 3485 3923 4856 4962 Size: 472 438 933 106 Fragments arranged by size: 945 933 789 472 438 356 323 292 228 106 53 27 AhdI GACnn_n'nnGTC Cuts at: 0 1274 4962 Size: 1274 3688 AleI CACnn'nnGTG Cuts at: 0 3185 4962 Size: 3185 1777 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4328 4962 Size: 4328 634 AlwNI CAG_nnn'CTG Cuts at: 0 1026 3463 4962 Size: 1026 2437 1499 Fragments arranged by size: 2437 1499 1026 ApaLI G'TGCA_C Cuts at: 0 2451 4962 Size: 2451 2511 ApoI r'AATT_y Cuts at: 0 272 478 508 794 1137 1439 1880 4156 Size: 272 206 30 286 343 302 441 2276 Cuts at: 4156 4962 Size: 806 Fragments arranged by size: 2276 806 441 343 302 286 272 206 30 AseI AT'TA_AT Cuts at: 0 1259 2969 3493 4900 4962 Size: 1259 1710 524 1407 62 Fragments arranged by size: 1710 1407 1259 524 62 BamHI G'GATC_C Cuts at: 0 3101 4135 4962 Size: 3101 1034 827 Fragments arranged by size: 3101 1034 827 BanII G_rGCy'C Cuts at: 0 152 3362 4962 Size: 152 3210 1600 Fragments arranged by size: 3210 1600 152 BbsI GAAGACnn'nnnn_ Cuts at: 0 344 590 1181 1598 1823 2251 2365 3002 Size: 344 246 591 417 225 428 114 637 Cuts at: 3002 3137 4962 Size: 135 1825 Fragments arranged by size: 1825 637 591 428 417 344 246 225 135 114 BclI T'GATC_A Cuts at: 0 974 2952 4962 Size: 974 1978 2010 Fragments arranged by size: 2010 1978 974 BfrBI ATG'CAT Cuts at: 0 4388 4392 4962 Size: 4388 4 570 Fragments arranged by size: 4388 570 4 BglII A'GATC_T Cuts at: 0 1737 4962 Size: 1737 3225 BlpI GC'TnA_GC Cuts at: 0 1340 3428 4962 Size: 1340 2088 1534 Fragments arranged by size: 2088 1534 1340 Bme1580I G_kGCm'C Cuts at: 0 1305 2455 3388 4962 Size: 1305 1150 933 1574 Fragments arranged by size: 1574 1305 1150 933 BmrI ACTGGGnnnn_n' Cuts at: 0 539 4962 Size: 539 4423 BmtI G_CTAG'C Cuts at: 0 3851 4962 Size: 3851 1111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 128 136 311 775 952 4496 4962 Size: 128 8 175 464 177 3544 466 Fragments arranged by size: 3544 466 464 177 175 128 8 Bpu10I CC'TnA_GC Cuts at: 0 203 2861 4962 Size: 203 2658 2101 Fragments arranged by size: 2658 2101 203 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2833 4962 Size: 2833 2129 BsaI GGTCTCn'nnnn_ Cuts at: 0 2682 3392 4962 Size: 2682 710 1570 Fragments arranged by size: 2682 1570 710 BsaAI yAC'GTr Cuts at: 0 1203 2068 4766 4962 Size: 1203 865 2698 196 Fragments arranged by size: 2698 1203 865 196 BsaBI GATnn'nnATC Cuts at: 0 428 3983 4962 Size: 428 3555 979 Fragments arranged by size: 3555 979 428 BsaWI w'CCGG_w Cuts at: 0 4672 4962 Size: 4672 290 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 121 151 2305 2335 4962 Size: 121 30 2154 30 2627 Fragments arranged by size: 2627 2154 121 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 129 1297 4083 4962 Size: 129 1168 2786 879 Fragments arranged by size: 2786 1168 879 129 BseYI C'CCAG_C Cuts at: 0 1659 2598 4962 Size: 1659 939 2364 Fragments arranged by size: 2364 1659 939 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2206 2229 4962 Size: 2206 23 2733 Fragments arranged by size: 2733 2206 23 BsiHKAI G_wGCw'C Cuts at: 0 152 2455 3182 3362 4962 Size: 152 2303 727 180 1600 Fragments arranged by size: 2303 1600 727 180 152 BsmI GAATG_Cn' Cuts at: 0 3846 4305 4962 Size: 3846 459 657 Fragments arranged by size: 3846 657 459 Bsp1286I G_dGCh'C Cuts at: 0 152 1305 2455 3182 3362 3388 4962 Size: 152 1153 1150 727 180 26 1574 Fragments arranged by size: 1574 1153 1150 727 180 152 26 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3417 4962 Size: 3417 1545 BsrDI GCAATG_nn' Cuts at: 0 2354 4962 Size: 2354 2608 BsrFI r'CCGG_y Cuts at: 0 2936 4962 Size: 2936 2026 BsrGI T'GTAC_A Cuts at: 0 1581 4602 4962 Size: 1581 3021 360 Fragments arranged by size: 3021 1581 360 BstAPI GCAn_nnn'nTGC Cuts at: 0 237 2089 4962 Size: 237 1852 2873 Fragments arranged by size: 2873 1852 237 BstBI TT'CG_AA Cuts at: 0 653 4962 Size: 653 4309 BstXI CCAn_nnnn'nTGG Cuts at: 0 613 3388 4770 4962 Size: 613 2775 1382 192 Fragments arranged by size: 2775 1382 613 192 BstYI r'GATC_y Cuts at: 0 516 1737 3101 4135 4962 Size: 516 1221 1364 1034 827 Fragments arranged by size: 1364 1221 1034 827 516 Bsu36I CC'TnA_GG Cuts at: 0 4335 4962 Size: 4335 627 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2607 4962 Size: 2607 2355 BtsI GCAGTG_nn' Cuts at: 0 24 1303 3612 3925 4569 4962 Size: 24 1279 2309 313 644 393 Fragments arranged by size: 2309 1279 644 393 313 24 DraI TTT'AAA Cuts at: 0 4430 4962 Size: 4430 532 DraIII CAC_nnn'GTG Cuts at: 0 1749 4962 Size: 1749 3213 EaeI y'GGCC_r Cuts at: 0 3353 3390 4962 Size: 3353 37 1572 Fragments arranged by size: 3353 1572 37 EarI CTCTTCn'nnn_ Cuts at: 0 44 56 1106 1598 2435 2725 2777 2804 Size: 44 12 1050 492 837 290 52 27 Cuts at: 2804 3461 4962 Size: 657 1501 Fragments arranged by size: 1501 1050 837 657 492 290 52 44 27 12 EcoICRI GAG'CTC Cuts at: 0 150 3360 4962 Size: 150 3210 1602 Fragments arranged by size: 3210 1602 150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 128 136 311 356 383 706 775 952 Size: 128 8 175 45 27 323 69 177 Cuts at: 952 1651 1943 2732 2785 3013 3485 3923 4496 Size: 699 292 789 53 228 472 438 573 Cuts at: 4496 4856 4962 Size: 360 106 Fragments arranged by size: 789 699 573 472 438 360 323 292 228 177 175 128 106 69 53 45 27 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 365 4232 4962 Size: 365 3867 730 Fragments arranged by size: 3867 730 365 EcoO109I rG'GnC_Cy Cuts at: 0 541 4530 4962 Size: 541 3989 432 Fragments arranged by size: 3989 541 432 EcoRI G'AATT_C Cuts at: 0 4156 4962 Size: 4156 806 EcoRV GAT'ATC Cuts at: 0 802 2696 4962 Size: 802 1894 2266 Fragments arranged by size: 2266 1894 802 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2379 3319 4962 Size: 2379 940 1643 Fragments arranged by size: 2379 1643 940 FspI TGC'GCA Cuts at: 0 3766 4962 Size: 3766 1196 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 121 124 153 156 1168 1200 1260 1292 Size: 121 3 29 3 1012 32 60 32 Cuts at: 1292 2305 2337 2781 2813 4962 Size: 1013 32 444 32 2149 Fragments arranged by size: 2149 1013 1012 444 121 60 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 0 1268 1551 3224 4962 Size: 1268 283 1673 1738 Fragments arranged by size: 1738 1673 1268 283 HindIII A'AGCT_T Cuts at: 0 1011 4962 Size: 1011 3951 MfeI C'AATT_G Cuts at: 0 208 4962 Size: 208 4754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2135 3129 3813 4962 Size: 2135 994 684 1149 Fragments arranged by size: 2135 1149 994 684 MscI TGG'CCA Cuts at: 0 3392 4962 Size: 3392 1570 MslI CAynn'nnrTG Cuts at: 0 1087 1481 3185 4356 4962 Size: 1087 394 1704 1171 606 Fragments arranged by size: 1704 1171 1087 606 394 MspA1I CmG'CkG Cuts at: 0 1026 1356 4312 4962 Size: 1026 330 2956 650 Fragments arranged by size: 2956 1026 650 330 NheI G'CTAG_C Cuts at: 0 3847 4962 Size: 3847 1115 NruI TCG'CGA Cuts at: 0 4406 4962 Size: 4406 556 NsiI A_TGCA'T Cuts at: 0 4390 4394 4962 Size: 4390 4 568 Fragments arranged by size: 4390 568 4 NspI r_CATG'y Cuts at: 0 123 1381 2092 3675 4132 4392 4962 Size: 123 1258 711 1583 457 260 570 Fragments arranged by size: 1583 1258 711 570 457 260 123 PflMI CCAn_nnn'nTGG Cuts at: 0 3607 4962 Size: 3607 1355 PfoI T'CCnGG_A Cuts at: 0 2766 4962 Size: 2766 2196 PmlI CAC'GTG Cuts at: 0 1203 4962 Size: 1203 3759 PpuMI rG'GwC_Cy Cuts at: 0 541 4530 4962 Size: 541 3989 432 Fragments arranged by size: 3989 541 432 PshAI GACnn'nnGTC Cuts at: 0 3065 4962 Size: 3065 1897 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3137 3169 4962 Size: 3137 32 1793 Fragments arranged by size: 3137 1793 32 PstI C_TGCA'G Cuts at: 0 169 699 1361 3711 4962 Size: 169 530 662 2350 1251 Fragments arranged by size: 2350 1251 662 530 169 PvuII CAG'CTG Cuts at: 0 1026 1356 4312 4962 Size: 1026 330 2956 650 Fragments arranged by size: 2956 1026 650 330 SacI G_AGCT'C Cuts at: 0 152 3362 4962 Size: 152 3210 1600 Fragments arranged by size: 3210 1600 152 SalI G'TCGA_C Cuts at: 0 3222 4962 Size: 3222 1740 SanDI GG'GwC_CC Cuts at: 0 541 4962 Size: 541 4421 SapI GCTCTTCn'nnn_ Cuts at: 0 1106 1598 2435 4962 Size: 1106 492 837 2527 Fragments arranged by size: 2527 1106 837 492 SfcI C'TryA_G Cuts at: 0 165 695 1345 1357 3707 4962 Size: 165 530 650 12 2350 1255 Fragments arranged by size: 2350 1255 650 530 165 12 SmlI C'TyrA_G Cuts at: 0 2812 4962 Size: 2812 2150 SphI G_CATG'C Cuts at: 0 2092 4392 4962 Size: 2092 2300 570 Fragments arranged by size: 2300 2092 570 SspI AAT'ATT Cuts at: 0 2550 3256 3772 4962 Size: 2550 706 516 1190 Fragments arranged by size: 2550 1190 706 516 StuI AGG'CCT Cuts at: 0 659 4962 Size: 659 4303 StyI C'CwwG_G Cuts at: 0 3393 3434 3559 3617 4201 4962 Size: 3393 41 125 58 584 761 Fragments arranged by size: 3393 761 584 125 58 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 356 4820 4962 Size: 356 4464 142 Fragments arranged by size: 4464 356 142 TatI w'GTAC_w Cuts at: 0 1326 1581 1647 3144 3638 4602 4858 4962 Size: 1326 255 66 1497 494 964 256 104 Fragments arranged by size: 1497 1326 964 494 256 255 104 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 93 125 4962 Size: 93 32 4837 Fragments arranged by size: 4837 93 32 XbaI T'CTAG_A Cuts at: 0 1278 2226 3057 4956 4962 Size: 1278 948 831 1899 6 Fragments arranged by size: 1899 1278 948 831 6 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2421 3400 4168 4927 4962 Size: 2421 979 768 759 35 Fragments arranged by size: 2421 979 768 759 35 XmnI GAAnn'nnTTC Cuts at: 0 1052 3023 3626 4298 4962 Size: 1052 1971 603 672 664 Fragments arranged by size: 1971 1052 672 664 603 Enzymes that do cut: AccI AcuI AhdI AleI AlfI AlwNI ApaLI ApoI AseI BamHI BanII BbsI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NheI NruI NsiI NspI PflMI PfoI PmlI PpuMI PshAI PsrI PstI PvuII SacI SalI SanDI SapI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AloI ApaI AscI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BglI BmgBI BplI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstEII BstZ17I BtgI ClaI CspCI DrdI EagI EciI FseI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV NotI PacI PasI PciI PmeI PpiI PsiI PspOMI PspXI PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI ZraI