(Circular) (Six-base) MAPSORT of: pFN21AB3277.seq Check: 6484 from: 1 to: 6520 pFN21AB3277 6520 bp With 182 enzymes: SgfI * September 25, 2018 16:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3863 1937 Size: 1926 4594 Acc65I G'GTAC_C Cuts at: 2671 3841 2671 Size: 1170 5350 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2732 3408 4583 5967 6235 1632 Size: 1100 676 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1175 1100 676 268 AfeI AGC'GCT Cuts at: 2748 2748 Size: 6520 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6323 AflIII A'CryG_T Cuts at: 1203 5419 6396 1203 Size: 4216 977 1327 Fragments arranged by size: 4216 1327 977 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 2690 5249 2690 Size: 2559 3961 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6520 AlwNI CAG_nnn'CTG Cuts at: 2246 4263 5835 2246 Size: 2017 1572 2931 Fragments arranged by size: 2931 2017 1572 ApaI G_GGCC'C Cuts at: 2705 2705 Size: 6520 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 2297 2420 2446 3829 4055 4207 6316 1493 Size: 804 123 26 1383 226 152 2109 1697 Fragments arranged by size: 2109 1697 1383 804 226 152 123 26 AseI AT'TA_AT Cuts at: 160 1032 2973 5074 160 Size: 872 1941 2101 1606 Fragments arranged by size: 2101 1941 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 3069 3666 3845 1870 Size: 73 1126 597 179 4545 Fragments arranged by size: 4545 1126 597 179 73 BamHI G'GATC_C Cuts at: 3850 4217 3850 Size: 367 6153 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2671 3657 3841 5296 618 Size: 325 217 627 884 986 184 1455 1842 Fragments arranged by size: 1842 1455 986 884 627 325 217 184 BanII G_rGCy'C Cuts at: 1391 2705 3839 1391 Size: 1314 1134 4072 Fragments arranged by size: 4072 1314 1134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2648 928 Size: 971 749 4800 Fragments arranged by size: 4800 971 749 BbvCI CC'TCA_GC Cuts at: 3442 3442 Size: 6520 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4711 4745 1903 Size: 34 50 34 2690 34 3678 Fragments arranged by size: 3678 2690 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2959 4412 5622 2959 Size: 1453 1210 3857 Fragments arranged by size: 3857 1453 1210 BclI T'GATC_A Cuts at: 1548 3340 1548 Size: 1792 4728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5131 136 Size: 107 122 71 631 605 3459 1525 Fragments arranged by size: 3459 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6515 6515 Size: 6520 BlpI GC'TnA_GC Cuts at: 2890 3937 2890 Size: 1047 5473 Bme1580I G_kGCm'C Cuts at: 1790 2705 4581 5737 1790 Size: 915 1876 1156 2573 Fragments arranged by size: 2573 1876 1156 915 BmgBI CAC'GTC Cuts at: 1318 3673 1318 Size: 2355 4165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2360 2577 2841 Size: 416 53 83 399 24 943 217 264 Cuts at: 2841 4251 5209 442 Size: 1410 958 1753 Fragments arranged by size: 1753 1410 958 943 416 399 264 217 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2413 3123 3230 3472 5180 1239 Size: 884 290 710 107 242 1708 2579 Fragments arranged by size: 2579 1708 884 710 290 242 107 Bpu10I CC'TnA_GC Cuts at: 3266 3442 3266 Size: 176 6344 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3066 3197 3318 4645 5510 5808 3066 Size: 131 121 1327 865 298 3778 Fragments arranged by size: 3778 1327 865 298 131 121 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3611 5183 882 Size: 617 2112 1572 2219 Fragments arranged by size: 2219 2112 1572 617 BsaAI yAC'GTr Cuts at: 493 1204 3541 3574 4311 493 Size: 711 2337 33 737 2702 Fragments arranged by size: 2702 2337 737 711 33 BsaBI GATnn'nnATC Cuts at: 2789 6514 2789 Size: 3725 2795 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4327 4709 5344 275 Size: 53 83 186 936 2794 382 635 1451 Fragments arranged by size: 2794 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4953 5351 5625 5772 6183 4953 Size: 398 274 147 411 5290 Fragments arranged by size: 5290 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 3220 3250 1178 Size: 30 165 30 1152 30 635 30 4448 Fragments arranged by size: 4448 1152 635 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2646 3430 1186 Size: 934 526 784 4276 Fragments arranged by size: 4276 934 784 526 BseYI C'CCAG_C Cuts at: 3239 3423 3785 5723 6219 3239 Size: 184 362 1938 496 3540 Fragments arranged by size: 3540 1938 496 362 184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5947 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3970 4731 5759 1540 Size: 275 186 1969 761 1028 2301 Fragments arranged by size: 2301 1969 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2652 3195 4581 4666 5737 2652 Size: 543 1386 85 1071 3435 Fragments arranged by size: 3435 1386 1071 543 85 BsmI GAATG_Cn' Cuts at: 2631 4031 4124 2631 Size: 1400 93 5027 Fragments arranged by size: 5027 1400 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3606 3606 Size: 6520 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2652 2705 3195 3839 4581 4666 5737 Size: 399 862 53 490 644 742 85 1071 Cuts at: 5737 1391 Size: 2174 Fragments arranged by size: 2174 1071 862 742 644 490 399 85 53 BspEI T'CCGG_A Cuts at: 6183 6183 Size: 6520 BspHI T'CATG_A Cuts at: 4409 6139 4409 Size: 1730 4790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3875 844 Size: 3031 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2140 2571 3362 3432 4407 1394 Size: 429 317 431 791 70 975 3507 Fragments arranged by size: 3507 975 791 431 429 317 70 BsrDI GCAATG_nn' Cuts at: 65 1248 2939 5015 5189 65 Size: 1183 1691 2076 174 1396 Fragments arranged by size: 2076 1691 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 5164 5351 1700 Size: 110 221 3133 187 2869 Fragments arranged by size: 3133 2869 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 4580 5592 4580 Size: 1012 5508 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6520 BstBI TT'CG_AA Cuts at: 2265 5324 2265 Size: 3059 3461 BstEII G'GTnAC_C Cuts at: 1184 2800 1184 Size: 1616 4904 BstXI CCAn_nnnn'nTGG Cuts at: 3467 3467 Size: 6520 BstYI r'GATC_y Cuts at: 1972 2870 3850 4217 4602 4619 6071 6157 6169 Size: 898 980 367 385 17 1452 86 12 Cuts at: 6169 6515 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 980 898 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3042 3042 Size: 6520 BtgI C'CryG_G Cuts at: 513 1065 3622 513 Size: 552 2557 3411 Fragments arranged by size: 3411 2557 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2012 3446 487 Size: 683 56 786 1434 3561 Fragments arranged by size: 3561 1434 786 683 56 BtsI GCAGTG_nn' Cuts at: 2015 2127 3472 3637 4036 4830 4850 2015 Size: 112 1345 165 399 794 20 3685 Fragments arranged by size: 3685 1345 794 399 165 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 2763 3824 2763 Size: 1061 5459 DraIII CAC_nnn'GTG Cuts at: 3596 3596 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5527 809 Size: 507 4211 1802 Fragments arranged by size: 4211 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 3967 4856 Size: 54 1425 50 275 18 423 1714 889 Cuts at: 4856 6485 8 Size: 1629 43 Fragments arranged by size: 1714 1629 1425 889 423 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3967 1537 Size: 275 2155 4090 Fragments arranged by size: 4090 2155 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3439 4450 1353 Size: 2086 1011 3423 Fragments arranged by size: 3423 2086 1011 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2797 3803 5107 5481 5627 387 Size: 1064 138 1208 1006 1304 374 146 1280 Fragments arranged by size: 1304 1280 1208 1064 1006 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2413 2732 3123 3230 3408 3472 Size: 393 491 290 319 391 107 178 64 Cuts at: 3472 4583 5180 5967 6235 1239 Size: 1111 597 787 268 1524 Fragments arranged by size: 1524 1111 787 597 491 393 391 319 290 268 178 107 64 EcoO109I rG'GnC_Cy Cuts at: 2702 2702 Size: 6520 EcoRI G'AATT_C Cuts at: 3829 4207 3829 Size: 378 6142 EcoRV GAT'ATC Cuts at: 2787 3765 2787 Size: 978 5542 FspI TGC'GCA Cuts at: 2126 5026 2126 Size: 2900 3620 HaeII r_GCGC'y Cuts at: 1678 2750 5667 1678 Size: 1072 2917 2531 Fragments arranged by size: 2917 2531 1072 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2676 2708 3022 3054 3337 Size: 32 135 32 1104 32 314 32 283 Cuts at: 3337 3369 5161 5193 5235 5267 1373 Size: 32 1792 32 42 32 2626 Fragments arranged by size: 2626 1792 1104 314 283 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3864 4110 669 Size: 988 281 1926 246 3079 Fragments arranged by size: 3079 1926 988 281 246 HindIII A'AGCT_T Cuts at: 748 2887 748 Size: 2139 4381 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 2675 3845 2675 Size: 1170 5350 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 2898 5634 5818 1305 Size: 834 759 2736 184 2007 Fragments arranged by size: 2736 2007 834 759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 10 Size: 54 1425 343 423 4275 Fragments arranged by size: 4275 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2229 2732 3465 4478 4837 4996 518 Size: 648 1063 503 733 1013 359 159 2042 Fragments arranged by size: 2042 1063 1013 733 648 503 359 159 MspA1I CmG'CkG Cuts at: 1606 2807 3116 3936 4613 5761 6006 1606 Size: 1201 309 820 677 1148 245 2120 Fragments arranged by size: 2120 1201 1148 820 677 309 245 NaeI GCC'GGC Cuts at: 2033 2033 Size: 6520 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5968 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NgoMIV G'CCGG_C Cuts at: 2031 2031 Size: 6520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 2288 3878 5423 1132 Size: 97 1059 1590 1545 2229 Fragments arranged by size: 2229 1590 1545 1059 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6520 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 2246 1108 Size: 1138 5382 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PmlI CAC'GTG Cuts at: 3574 3574 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4589 4621 6102 6134 4589 Size: 32 1481 32 4975 Fragments arranged by size: 4975 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2204 1613 Size: 591 5929 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PspOMI G'GGCC_C Cuts at: 2701 2701 Size: 6520 PstI C_TGCA'G Cuts at: 830 2394 3872 830 Size: 1564 1478 3478 Fragments arranged by size: 3478 1564 1478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 3116 3116 Size: 6520 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SapI GCTCTTCn'nnn_ Cuts at: 3439 3439 Size: 6520 SbfI CC_TGCA'GG Cuts at: 3872 3872 Size: 6520 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SfcI C'TryA_G Cuts at: 826 1045 2212 2390 2511 3868 5003 5684 5875 Size: 219 1167 178 121 1357 1135 681 191 Cuts at: 5875 826 Size: 1471 Fragments arranged by size: 1471 1357 1167 1135 681 219 191 178 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 2031 1700 Size: 331 6189 SmaI CCC'GGG Cuts at: 1872 3668 3847 1872 Size: 1796 179 4545 Fragments arranged by size: 4545 1796 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 3069 3081 3176 3333 4624 5525 Size: 197 926 1126 12 95 157 1291 901 Cuts at: 5525 5787 820 Size: 262 1553 Fragments arranged by size: 1553 1291 1126 926 901 262 197 157 95 12 SnaBI TAC'GTA Cuts at: 493 3541 4311 493 Size: 3048 770 2702 Fragments arranged by size: 3048 2702 770 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 3215 1517 Size: 1698 4822 StyI C'CwwG_G Cuts at: 513 1065 3003 3729 3959 513 Size: 552 1938 726 230 3074 Fragments arranged by size: 3074 1938 726 552 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 3245 4547 4564 4717 4902 5347 Size: 1255 873 792 1302 17 153 185 445 Cuts at: 5347 325 Size: 1498 Fragments arranged by size: 1498 1302 1255 873 792 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4766 6478 96 Size: 274 80 33 51 1443 2789 1712 138 Fragments arranged by size: 2789 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 3220 3252 3419 3451 3635 3667 6480 Size: 32 1126 32 167 32 184 32 2813 Cuts at: 6480 6512 2062 Size: 32 2070 Fragments arranged by size: 2813 2070 1126 184 167 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6520 XbaI T'CTAG_A Cuts at: 3856 3856 Size: 6520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2686 2686 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 3069 1943 Size: 1126 5394 XmaI C'CCGG_G Cuts at: 1870 3666 3845 1870 Size: 1796 179 4545 Fragments arranged by size: 4545 1796 179 XmnI GAAnn'nnTTC Cuts at: 4649 4649 Size: 6520 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI