################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StyI SwaI TfiI Tru9I Tsp45I TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 562 GAATTC [GA]^AATT[CT] AluI 608 AGCT AG^CT ApoI 562 GAATTC [GA]^AATT[CT] AscI 173 GGCGCGCC GG^CGCGCC AspHI 661 GTGCTC G[AT]GC[AT]^C AvaI 774 CCCGAG C^[CT]CG[GA]G BfaI 158 CTAG C^TAG BglI 4 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 262 CTGGAG CTGGAG(16/14) BsaI 619 GAGACC GGTCTC(1/5) BsiHKAI 661 GTGCTC G[AT]GC[AT]^C BsmAI 619 GAGAC GTCTC(1/5) BsmI 612 GCATTC GAATGC(1/-1) BsoBI 774 CCCGAG C^[CT]CG[GA]G BsrBI 628 GAGCGG CCGCTC(-3/-3) BstF5I 533 GGATG GGATG(2/0) DpnII 673 GATC ^GATC EarI 634 CTCTTC CTCTTC(1/4) EcoRI 562 GAATTC G^AATTC FokI 533 GGATG GGATG(9/13) HincII 373 GTCAAC GT[CT]^[GA]AC HindII 373 GTCAAC GT[CT]^[GA]AC HinfI 313 GAGTC G^A[ACGT]TC HphI 665 TCACC GGTGA(8/7) Ksp632I 634 CTCTTC CTCTTC(1/4) KspI 5 CCGCGG CCGC^GG MaeI 158 CTAG C^TAG MboI 673 GATC ^GATC NdeII 673 GATC ^GATC PflMI 750 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 313 GAGTC GAGTC(4/5) PvuII 607 CAGCTG CAG^CTG SacII 5 CCGCGG CCGC^GG SapI 633 GCTCTTC GCTCTTC(1/4) Sau3AI 673 GATC ^GATC SbfI 134 CCTGCAGG CCTGCA^GG SfaNI 534 GATGC GCATC(5/9) Sse8387I 134 CCTGCAGG CCTGCA^GG StuI 131 AGGCCT AGG^CCT Tsp509I 563 AATT ^AATT Van91I 750 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 399 GTAC GT^AC 648 GTAC BsiEI 166 CGGCCG CG[GA][CT]^CG 480 CGGCCG BsrFI 294 GCCGGC [GA]^CCGG[CT] 576 GCCGGC BssHII 107 GCGCGC G^CGCGC 174 GCGCGC BstAPI 330 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 702 GCAGGTCTTGC Cfr10I 294 GCCGGC [GA]^CCGG[CT] 576 GCCGGC DdeI 16 CTGAG C^T[ACGT]AG 739 CTGAG DsaI 5 CCGCGG C^C[GA][CT]GG 354 CCGTGG EagI 166 CGGCCG C^GGCCG 480 CGGCCG EclXI 166 CGGCCG C^GGCCG 480 CGGCCG Eco52I 166 CGGCCG C^GGCCG 480 CGGCCG EcoNI 429 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 738 CCTGAGCCAGG MaeII 371 ACGT A^CGT 494 ACGT MboII 720 GAAGA GAAGA(8/7) 635 TCTTC NaeI 294 GCCGGC GCC^GGC 576 GCCGGC NgoMIV 294 GCCGGC G^CCGGC 576 GCCGGC PstI 135 CTGCAG CTGCA^G 454 CTGCAG RsaI 399 GTAC GT^AC 648 GTAC SfcI 135 CTGCAG C^T[GA][CT]AG 454 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AlwNI ApaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV PpuMI Sau96I ScrFI SfoI TaqI TseI TthHB8I