(Linear) (Six-base) MAPSORT of: KIEE2711.seq Check: 675 from: 1 to: 2793 KIEE2711.orf With 182 enzymes: SgfI * January 19, 2018 17:31 .. AccI GT'mk_AC Cuts at: 0 1355 2793 Size: 1355 1438 Acc65I G'GTAC_C Cuts at: 0 2536 2793 Size: 2536 257 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 782 911 1217 2743 2793 Size: 637 145 129 306 1526 50 Fragments arranged by size: 1526 637 306 145 129 50 AgeI A'CCGG_T Cuts at: 0 1661 2793 Size: 1661 1132 AleI CACnn'nnGTG Cuts at: 0 2122 2793 Size: 2122 671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1034 1066 2793 Size: 1034 32 1727 Fragments arranged by size: 1727 1034 32 ApoI r'AATT_y Cuts at: 0 63 721 1006 1342 2793 Size: 63 658 285 336 1451 Fragments arranged by size: 1451 658 336 285 63 AvaI C'yCGr_G Cuts at: 0 95 2194 2576 2793 Size: 95 2099 382 217 Fragments arranged by size: 2099 382 217 95 BanI G'GyrC_C Cuts at: 0 2020 2536 2793 Size: 2020 516 257 Fragments arranged by size: 2020 516 257 BanII G_rGCy'C Cuts at: 0 958 1077 1271 1382 2793 Size: 958 119 194 111 1411 Fragments arranged by size: 1411 958 194 119 111 BbeI G_GCGC'C Cuts at: 0 2024 2793 Size: 2024 769 BbsI GAAGACnn'nnnn_ Cuts at: 0 904 2079 2479 2793 Size: 904 1175 400 314 Fragments arranged by size: 1175 904 400 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2175 2209 2793 Size: 2175 34 584 Fragments arranged by size: 2175 584 34 BciVI GTATCCnnnnn_n' Cuts at: 0 520 2793 Size: 520 2273 BglI GCCn_nnn'nGGC Cuts at: 0 1147 2793 Size: 1147 1646 BlpI GC'TnA_GC Cuts at: 0 1654 2793 Size: 1654 1139 Bme1580I G_kGCm'C Cuts at: 0 2268 2793 Size: 2268 525 BmrI ACTGGGnnnn_n' Cuts at: 0 645 1082 1534 2782 2793 Size: 645 437 452 1248 11 Fragments arranged by size: 1248 645 452 437 11 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 570 1825 1975 2793 Size: 570 1255 150 818 Fragments arranged by size: 1255 818 570 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 2542 2793 Size: 227 2315 251 Fragments arranged by size: 2315 251 227 Bpu10I CC'TnA_GC Cuts at: 0 1943 2793 Size: 1943 850 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 994 1566 2793 Size: 994 572 1227 Fragments arranged by size: 1227 994 572 BsaI GGTCTCn'nnnn_ Cuts at: 0 253 2586 2793 Size: 253 2333 207 Fragments arranged by size: 2333 253 207 BsaAI yAC'GTr Cuts at: 0 2177 2793 Size: 2177 616 BsaBI GATnn'nnATC Cuts at: 0 205 2793 Size: 205 2588 BsaHI Gr'CG_yC Cuts at: 0 2021 2793 Size: 2021 772 BsaWI w'CCGG_w Cuts at: 0 1363 1661 2793 Size: 1363 298 1132 Fragments arranged by size: 1363 1132 298 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 147 177 1306 1336 1814 1844 2793 Size: 147 30 1129 30 478 30 949 Fragments arranged by size: 1129 949 478 147 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 42 1361 1564 1567 1964 2793 Size: 42 1319 203 3 397 829 Fragments arranged by size: 1319 829 397 203 42 3 BseYI C'CCAG_C Cuts at: 0 756 1102 1422 2250 2793 Size: 756 346 320 828 543 Fragments arranged by size: 828 756 543 346 320 BsiEI CG_ry'CG Cuts at: 0 1710 2049 2793 Size: 1710 339 744 Fragments arranged by size: 1710 744 339 BsiHKAI G_wGCw'C Cuts at: 0 1101 1271 2793 Size: 1101 170 1522 Fragments arranged by size: 1522 1101 170 BsmI GAATG_Cn' Cuts at: 0 2186 2793 Size: 2186 607 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1018 2686 2793 Size: 1018 1668 107 Fragments arranged by size: 1668 1018 107 Bsp1286I G_dGCh'C Cuts at: 0 958 1077 1101 1271 1382 2268 2793 Size: 958 119 24 170 111 886 525 Fragments arranged by size: 958 886 525 170 119 111 24 BspEI T'CCGG_A Cuts at: 0 1363 2793 Size: 1363 1430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1947 2793 Size: 1947 846 BsrBI CCG'CTC Cuts at: 0 241 1322 2793 Size: 241 1081 1471 Fragments arranged by size: 1471 1081 241 BsrFI r'CCGG_y Cuts at: 0 1661 2793 Size: 1661 1132 BssSI C'ACGA_G Cuts at: 0 1270 1375 2793 Size: 1270 105 1418 Fragments arranged by size: 1418 1270 105 BstAPI GCAn_nnn'nTGC Cuts at: 0 1471 2793 Size: 1471 1322 BstEII G'GTnAC_C Cuts at: 0 324 1533 2317 2793 Size: 324 1209 784 476 Fragments arranged by size: 1209 784 476 324 BstXI CCAn_nnnn'nTGG Cuts at: 0 1176 1296 2793 Size: 1176 120 1497 Fragments arranged by size: 1497 1176 120 BstYI r'GATC_y Cuts at: 0 2525 2640 2793 Size: 2525 115 153 Fragments arranged by size: 2525 153 115 BtgI C'CryG_G Cuts at: 0 577 1175 1326 2560 2793 Size: 577 598 151 1234 233 Fragments arranged by size: 1234 598 577 233 151 BtsI GCAGTG_nn' Cuts at: 0 967 1073 2000 2793 Size: 967 106 927 793 Fragments arranged by size: 967 927 793 106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 828 863 954 989 2132 2167 2793 Size: 828 35 91 35 1143 35 626 Fragments arranged by size: 1143 828 626 91 35 35 35 DraI TTT'AAA Cuts at: 0 720 2793 Size: 720 2073 DraIII CAC_nnn'GTG Cuts at: 0 526 2793 Size: 526 2267 DrdI GACnn_nn'nnGTC Cuts at: 0 906 1759 2793 Size: 906 853 1034 Fragments arranged by size: 1034 906 853 EaeI y'GGCC_r Cuts at: 0 2 468 741 782 1127 1707 1721 2696 Size: 2 466 273 41 345 580 14 975 Cuts at: 2696 2793 Size: 97 Fragments arranged by size: 975 580 466 345 273 97 41 14 2 EagI C'GGCC_G Cuts at: 0 1707 2793 Size: 1707 1086 EarI CTCTTCn'nnn_ Cuts at: 0 40 946 2793 Size: 40 906 1847 Fragments arranged by size: 1847 906 40 EcoICRI GAG'CTC Cuts at: 0 1269 2793 Size: 1269 1524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 637 782 911 1217 2542 2743 2793 Size: 227 410 145 129 306 1325 201 50 Fragments arranged by size: 1325 410 306 227 201 145 129 50 EcoNI CCTnn'n_nnAGG Cuts at: 0 88 2793 Size: 88 2705 EcoO109I rG'GnC_Cy Cuts at: 0 1030 1149 1586 2793 Size: 1030 119 437 1207 Fragments arranged by size: 1207 1030 437 119 EcoRI G'AATT_C Cuts at: 0 1006 1342 2793 Size: 1006 336 1451 Fragments arranged by size: 1451 1006 336 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2724 2793 Size: 2724 69 FspI TGC'GCA Cuts at: 0 1434 2793 Size: 1434 1359 HaeII r_GCGC'y Cuts at: 0 2024 2793 Size: 2024 769 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 147 179 874 906 1599 1631 1814 1846 Size: 147 32 695 32 693 32 183 32 Cuts at: 1846 1856 1888 2793 Size: 10 32 905 Fragments arranged by size: 905 695 693 183 147 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 0 456 1356 2273 2418 2493 2793 Size: 456 900 917 145 75 300 Fragments arranged by size: 917 900 456 300 145 75 KasI G'GCGC_C Cuts at: 0 2020 2793 Size: 2020 773 KpnI G_GTAC'C Cuts at: 0 2540 2793 Size: 2540 253 MscI TGG'CCA Cuts at: 0 470 784 2793 Size: 470 314 2009 Fragments arranged by size: 2009 470 314 MslI CAynn'nnrTG Cuts at: 0 428 547 1732 2122 2793 Size: 428 119 1185 390 671 Fragments arranged by size: 1185 671 428 390 119 MspA1I CmG'CkG Cuts at: 0 247 615 632 760 2793 Size: 247 368 17 128 2033 Fragments arranged by size: 2033 368 247 128 17 NarI GG'CG_CC Cuts at: 0 2021 2793 Size: 2021 772 NcoI C'CATG_G Cuts at: 0 1175 1326 2793 Size: 1175 151 1467 Fragments arranged by size: 1467 1175 151 NdeI CA'TA_TG Cuts at: 0 169 2793 Size: 169 2624 PfoI T'CCnGG_A Cuts at: 0 1858 2467 2528 2793 Size: 1858 609 61 265 Fragments arranged by size: 1858 609 265 61 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1369 1401 2793 Size: 1369 32 1392 Fragments arranged by size: 1392 1369 32 PpuMI rG'GwC_Cy Cuts at: 0 1586 2793 Size: 1586 1207 PshAI GACnn'nnGTC Cuts at: 0 2488 2793 Size: 2488 305 PstI C_TGCA'G Cuts at: 0 906 2044 2288 2793 Size: 906 1138 244 505 Fragments arranged by size: 1138 906 505 244 PvuII CAG'CTG Cuts at: 0 247 615 760 2793 Size: 247 368 145 2033 Fragments arranged by size: 2033 368 247 145 SacI G_AGCT'C Cuts at: 0 1271 2793 Size: 1271 1522 SalI G'TCGA_C Cuts at: 0 1354 2793 Size: 1354 1439 SapI GCTCTTCn'nnn_ Cuts at: 0 946 2793 Size: 946 1847 SexAI A'CCwGG_T Cuts at: 0 522 2793 Size: 522 2271 SfcI C'TryA_G Cuts at: 0 144 561 902 1884 2040 2220 2284 2793 Size: 144 417 341 982 156 180 64 509 Fragments arranged by size: 982 509 417 341 180 156 144 64 SfoI GGC'GCC Cuts at: 0 2022 2793 Size: 2022 771 SgrAI Cr'CCGG_yG Cuts at: 0 1661 2793 Size: 1661 1132 SmaI CCC'GGG Cuts at: 0 2578 2793 Size: 2578 215 SmlI C'TyrA_G Cuts at: 0 973 1581 2793 Size: 973 608 1212 Fragments arranged by size: 1212 973 608 SspI AAT'ATT Cuts at: 0 78 2793 Size: 78 2715 StuI AGG'CCT Cuts at: 0 2720 2793 Size: 2720 73 StyI C'CwwG_G Cuts at: 0 89 839 1175 1326 2120 2331 2426 2793 Size: 89 750 336 151 794 211 95 367 Fragments arranged by size: 794 750 367 336 211 151 95 89 SwaI ATTT'AAAT Cuts at: 0 720 2793 Size: 720 2073 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1288 1747 2793 Size: 1288 459 1046 Fragments arranged by size: 1288 1046 459 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1776 1808 2793 Size: 1776 32 985 Fragments arranged by size: 1776 985 32 Tth111I GACn'n_nGTC Cuts at: 0 1977 2793 Size: 1977 816 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 336 1110 1402 2428 2547 2793 Size: 336 774 292 1026 119 246 Fragments arranged by size: 1026 774 336 292 246 119 XmaI C'CCGG_G Cuts at: 0 2576 2793 Size: 2576 217 XmnI GAAnn'nnTTC Cuts at: 0 2707 2793 Size: 2707 86 Enzymes that do cut: AccI Acc65I AcuI AgeI AleI AloI ApoI AvaI BanI BanII BbeI BbsI BcgI BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NarI NcoI NdeI PfoI PpiI PpuMI PshAI PstI PvuII SacI SalI SapI SexAI SfcI SfoI SgrAI SmaI SmlI SspI StuI StyI SwaI TaqII TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AhdI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI BglII BmgBI BmtI BsgI BsiWI BspHI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI EciI EcoRV FseI FspAI HindIII HpaI MfeI MluI MmeI NaeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SbfI ScaI SfiI SgfI SnaBI SpeI SphI SrfI TatI XbaI XhoI ZraI