################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AclI AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvrII BclI BlnI BsgI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI Eam1105I Eco47III Eco81I EcoRV EcoT22I FbaI FseI HindIII HpaI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PmeI PshBI Psp1406I PvuI RcaI RsrII SbfI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 3421 GGTACC G^GTACC AccI 2239 GTCGAC GT^[AC][GT]AC AccIII 2248 TCCGGA T^CCGGA AcyI 2905 GGCGCC G[GA]^CG[CT]C AflII 121 CTTAAG C^TTAAG AlwNI 391 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 3421 GGTACC G^GTACC AviII 2317 TGCGCA TGC^GCA BamHI 802 GGATCC G^GATCC BbeI 2905 GGCGCC GGCGC^C BbrPI 338 CACGTG CAC^GTG BbvCI 24 CCTCAGC CCTCAGC(-5/-2) BcgI 3071 GCATTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 121 CTTAAG C^TTAAG BglI 2026 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 717 AGATCT A^GATCT BlpI 2538 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2538 GCTGAGC GC^T[ACGT]AGC BsaBI 1086 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2905 GGCGCC G[GA]^CG[CT]C BseAI 2248 TCCGGA T^CCGGA BsmI 3071 GCATTC GAATGC(1/-1) BspEI 2248 TCCGGA T^CCGGA BspMI 2823 ACCTGC ACCTGC(4/8) BstAPI 2350 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 2538 GCTGAGC GC^T[ACGT]AGC DraI 1603 TTTAAA TTT^AAA EagI 2592 CGGCCG C^GGCCG EclXI 2592 CGGCCG C^GGCCG Eco52I 2592 CGGCCG C^GGCCG FspI 2317 TGCGCA TGC^GCA HaeII 2905 GGCGCC [GA]GCGC^[CT] HgaI 3374 GCGTC GACGC(5/10) Hin1I 2905 GGCGCC G[GA]^CG[CT]C KasI 2905 GGCGCC G^GCGCC KpnI 3421 GGTACC GGTAC^C KspI 7 CCGCGG CCGC^GG MamI 1086 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2248 TCCGGA T^CCGGA NarI 2905 GGCGCC GG^CGCC NdeI 1053 CATATG CA^TATG PmaCI 338 CACGTG CAC^GTG PmlI 338 CACGTG CAC^GTG PpuMI 2470 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 3369 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2152 GAGCTC GAGCT^C SacII 7 CCGCGG CCGC^GG SalI 2239 GTCGAC G^TCGAC ScaI 228 AGTACT AGT^ACT SexAI 1407 ACCAGGT A^CC[AT]GGT SfoI 2905 GGCGCC GGC^GCC SmaI 3461 CCCGGG CCC^GGG SspI 961 AATATT AAT^ATT StuI 3603 AGGCCT AGG^CCT SwaI 1602 ATTTAAAT ATTT^AAAT XmaCI 3461 CCCGGG C^CCGGG XmaI 3461 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AgeI 162 ACCGGT A^CCGGT 2546 ACCGGT Asp700I 715 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3588 GAACTACTTC AspI 180 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2859 GACTGTGTC BalI 1353 TGGCCA TGG^CCA 1667 TGGCCA BanI 2905 GGCGCC G^G[CT][GA]CC 3421 GGTACC BciVI 31 GGATAC GTATCC(6/5) 1394 GTATCC BfaI 1544 CTAG C^TAG 1708 CTAG BsaAI 338 CACGTG [CT]AC^GT[GA] 3060 TACGTG BsiEI 2592 CGGCCG CG[GA][CT]^CG 2931 CGACCG BsmBI 1897 CGTCTC CGTCTC(1/5) 3577 GAGACG BsrBI 1124 CCGCTC CCGCTC(-3/-3) 2205 CCGCTC BsrFI 162 ACCGGT [GA]^CCGG[CT] 2546 ACCGGT BssSI 2260 CACGAG CACGAG(-5/-1) 2155 CTCGTG Cfr10I 162 ACCGGT [GA]^CCGG[CT] 2546 ACCGGT DraIII 335 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 1406 CACCAGGTG EcoNI 88 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 969 CCTTACCAAGG EcoRI 1891 GAATTC G^AATTC 2227 GAATTC MaeI 1544 CTAG C^TAG 1708 CTAG MluNI 1353 TGGCCA TGG^CCA 1667 TGGCCA MscI 1353 TGGCCA TGG^CCA 1667 TGGCCA NcoI 2060 CCATGG C^CATGG 2211 CCATGG PflFI 180 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2859 GACTGTGTC PinAI 162 ACCGGT A^CCGGT 2546 ACCGGT SapI 442 GAAGAGC GCTCTTC(1/4) 1836 GAAGAGC SgrAI 161 CACCGGTG C[GA]^CCGG[CT]G 2545 CACCGGTG Tth111I 180 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2859 GACTGTGTC XmnI 715 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3588 GAACTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII