(Linear) (Six-base) MAPSORT of: KIEE9667.seq Check: 5730 from: 1 to: 3678 KIEE9667.orf With 182 enzymes: SgfI * September 1, 2019 16:00 .. AccI GT'mk_AC Cuts at: 0 2240 3678 Size: 2240 1438 Acc65I G'GTAC_C Cuts at: 0 3421 3678 Size: 3421 257 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 854 1522 1667 1796 2102 3628 3678 Size: 854 668 145 129 306 1526 50 Fragments arranged by size: 1526 854 668 306 145 129 50 AflII C'TTAA_G Cuts at: 0 121 3678 Size: 121 3557 AgeI A'CCGG_T Cuts at: 0 162 2546 3678 Size: 162 2384 1132 Fragments arranged by size: 2384 1132 162 AleI CACnn'nnGTG Cuts at: 0 3007 3678 Size: 3007 671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 367 3678 Size: 367 3311 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1919 1951 3678 Size: 1919 32 1727 Fragments arranged by size: 1919 1727 32 AlwNI CAG_nnn'CTG Cuts at: 0 396 3678 Size: 396 3282 ApoI r'AATT_y Cuts at: 0 133 948 1606 1891 2227 3678 Size: 133 815 658 285 336 1451 Fragments arranged by size: 1451 815 658 336 285 133 AvaI C'yCGr_G Cuts at: 0 672 980 3079 3461 3678 Size: 672 308 2099 382 217 Fragments arranged by size: 2099 672 382 308 217 BamHI G'GATC_C Cuts at: 0 802 3678 Size: 802 2876 BanI G'GyrC_C Cuts at: 0 2905 3421 3678 Size: 2905 516 257 Fragments arranged by size: 2905 516 257 BanII G_rGCy'C Cuts at: 0 311 660 1843 1962 2156 2267 3678 Size: 311 349 1183 119 194 111 1411 Fragments arranged by size: 1411 1183 349 311 194 119 111 BbeI G_GCGC'C Cuts at: 0 2909 3678 Size: 2909 769 BbsI GAAGACnn'nnnn_ Cuts at: 0 695 842 1789 2964 3364 3678 Size: 695 147 947 1175 400 314 Fragments arranged by size: 1175 947 695 400 314 147 BbvCI CC'TCA_GC Cuts at: 0 25 3678 Size: 25 3653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3060 3094 3678 Size: 3060 34 584 Fragments arranged by size: 3060 584 34 BciVI GTATCCnnnnn_n' Cuts at: 0 25 1405 3678 Size: 25 1380 2273 Fragments arranged by size: 2273 1380 25 BglI GCCn_nnn'nGGC Cuts at: 0 2032 3678 Size: 2032 1646 BglII A'GATC_T Cuts at: 0 717 3678 Size: 717 2961 BlpI GC'TnA_GC Cuts at: 0 2539 3678 Size: 2539 1139 Bme1580I G_kGCm'C Cuts at: 0 3153 3678 Size: 3153 525 BmrI ACTGGGnnnn_n' Cuts at: 0 101 1530 1967 2419 3667 3678 Size: 101 1429 437 452 1248 11 Fragments arranged by size: 1429 1248 452 437 101 11 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1455 2710 2860 3678 Size: 1455 1255 150 818 Fragments arranged by size: 1455 1255 818 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 295 1112 3427 3678 Size: 225 70 817 2315 251 Fragments arranged by size: 2315 817 251 225 70 Bpu10I CC'TnA_GC Cuts at: 0 25 749 2828 3678 Size: 25 724 2079 850 Fragments arranged by size: 2079 850 724 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 745 892 1879 2451 3678 Size: 124 621 147 987 572 1227 Fragments arranged by size: 1227 987 621 572 147 124 BsaI GGTCTCn'nnnn_ Cuts at: 0 600 1138 3471 3678 Size: 600 538 2333 207 Fragments arranged by size: 2333 600 538 207 BsaAI yAC'GTr Cuts at: 0 340 3062 3678 Size: 340 2722 616 Fragments arranged by size: 2722 616 340 BsaBI GATnn'nnATC Cuts at: 0 1090 3678 Size: 1090 2588 BsaHI Gr'CG_yC Cuts at: 0 2906 3678 Size: 2906 772 BsaWI w'CCGG_w Cuts at: 0 162 684 2248 2546 3678 Size: 162 522 1564 298 1132 Fragments arranged by size: 1564 1132 522 298 162 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 185 215 682 712 829 859 1032 1062 Size: 185 30 467 30 117 30 173 30 Cuts at: 1062 2191 2221 2699 2729 3678 Size: 1129 30 478 30 949 Fragments arranged by size: 1129 949 478 467 185 173 117 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 927 2246 2449 2452 2849 3678 Size: 927 1319 203 3 397 829 Fragments arranged by size: 1319 927 829 397 203 3 BseYI C'CCAG_C Cuts at: 0 1641 1987 2307 3135 3678 Size: 1641 346 320 828 543 Fragments arranged by size: 1641 828 543 346 320 BsiEI CG_ry'CG Cuts at: 0 2595 2934 3678 Size: 2595 339 744 Fragments arranged by size: 2595 744 339 BsiHKAI G_wGCw'C Cuts at: 0 65 1986 2156 3678 Size: 65 1921 170 1522 Fragments arranged by size: 1921 1522 170 65 BsmI GAATG_Cn' Cuts at: 0 3071 3678 Size: 3071 607 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1903 3571 3678 Size: 1903 1668 107 Fragments arranged by size: 1903 1668 107 Bsp1286I G_dGCh'C Cuts at: 0 65 311 660 1843 1962 1986 2156 2267 Size: 65 246 349 1183 119 24 170 111 Cuts at: 2267 3153 3678 Size: 886 525 Fragments arranged by size: 1183 886 525 349 246 170 119 111 65 24 BspEI T'CCGG_A Cuts at: 0 2248 3678 Size: 2248 1430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2832 3678 Size: 2832 846 BsrBI CCG'CTC Cuts at: 0 1126 2207 3678 Size: 1126 1081 1471 Fragments arranged by size: 1471 1126 1081 BsrFI r'CCGG_y Cuts at: 0 162 2546 3678 Size: 162 2384 1132 Fragments arranged by size: 2384 1132 162 BssSI C'ACGA_G Cuts at: 0 2155 2260 3678 Size: 2155 105 1418 Fragments arranged by size: 2155 1418 105 BstAPI GCAn_nnn'nTGC Cuts at: 0 2356 3678 Size: 2356 1322 BstEII G'GTnAC_C Cuts at: 0 1209 2418 3202 3678 Size: 1209 1209 784 476 Fragments arranged by size: 1209 1209 784 476 BstXI CCAn_nnnn'nTGG Cuts at: 0 88 2061 2181 3678 Size: 88 1973 120 1497 Fragments arranged by size: 1973 1497 120 88 BstYI r'GATC_y Cuts at: 0 97 425 717 802 864 3410 3525 3678 Size: 97 328 292 85 62 2546 115 153 Fragments arranged by size: 2546 328 292 153 115 97 85 62 BtgI C'CryG_G Cuts at: 0 7 1462 2060 2211 3445 3678 Size: 7 1455 598 151 1234 233 Fragments arranged by size: 1455 1234 598 233 151 7 BtsI GCAGTG_nn' Cuts at: 0 187 1852 1958 2885 3678 Size: 187 1665 106 927 793 Fragments arranged by size: 1665 927 793 187 106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1713 1748 1839 1874 3017 3052 3678 Size: 1713 35 91 35 1143 35 626 Fragments arranged by size: 1713 1143 626 91 35 35 35 DraI TTT'AAA Cuts at: 0 1605 3678 Size: 1605 2073 DraIII CAC_nnn'GTG Cuts at: 0 340 1411 3678 Size: 340 1071 2267 Fragments arranged by size: 2267 1071 340 DrdI GACnn_nn'nnGTC Cuts at: 0 641 788 1791 2644 3678 Size: 641 147 1003 853 1034 Fragments arranged by size: 1034 1003 853 641 147 EaeI y'GGCC_r Cuts at: 0 214 887 1353 1626 1667 2012 2592 2606 Size: 214 673 466 273 41 345 580 14 Cuts at: 2606 3581 3678 Size: 975 97 Fragments arranged by size: 975 673 580 466 345 273 214 97 41 14 EagI C'GGCC_G Cuts at: 0 2592 3678 Size: 2592 1086 EarI CTCTTCn'nnn_ Cuts at: 0 437 925 1831 3678 Size: 437 488 906 1847 Fragments arranged by size: 1847 906 488 437 EcoICRI GAG'CTC Cuts at: 0 2154 3678 Size: 2154 1524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 295 854 1112 1522 1667 1796 2102 Size: 225 70 559 258 410 145 129 306 Cuts at: 2102 3427 3628 3678 Size: 1325 201 50 Fragments arranged by size: 1325 559 410 306 258 225 201 145 129 70 50 EcoNI CCTnn'n_nnAGG Cuts at: 0 92 973 3678 Size: 92 881 2705 Fragments arranged by size: 2705 881 92 EcoO109I rG'GnC_Cy Cuts at: 0 1915 2034 2471 3678 Size: 1915 119 437 1207 Fragments arranged by size: 1915 1207 437 119 EcoRI G'AATT_C Cuts at: 0 1891 2227 3678 Size: 1891 336 1451 Fragments arranged by size: 1891 1451 336 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 739 886 3609 3678 Size: 739 147 2723 69 Fragments arranged by size: 2723 739 147 69 FspI TGC'GCA Cuts at: 0 2319 3678 Size: 2319 1359 HaeII r_GCGC'y Cuts at: 0 2909 3678 Size: 2909 769 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 682 714 829 861 1032 1064 1759 1791 Size: 682 32 115 32 171 32 695 32 Cuts at: 1791 2484 2516 2699 2731 2741 2773 3678 Size: 693 32 183 32 10 32 905 Fragments arranged by size: 905 695 693 682 183 171 115 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 0 1341 2241 3158 3303 3378 3678 Size: 1341 900 917 145 75 300 Fragments arranged by size: 1341 917 900 300 145 75 KasI G'GCGC_C Cuts at: 0 2905 3678 Size: 2905 773 KpnI G_GTAC'C Cuts at: 0 3425 3678 Size: 3425 253 MscI TGG'CCA Cuts at: 0 1355 1669 3678 Size: 1355 314 2009 Fragments arranged by size: 2009 1355 314 MslI CAynn'nnrTG Cuts at: 0 1313 1432 2617 3007 3678 Size: 1313 119 1185 390 671 Fragments arranged by size: 1313 1185 671 390 119 MspA1I CmG'CkG Cuts at: 0 9 29 623 770 1132 1500 1517 1645 Size: 9 20 594 147 362 368 17 128 Cuts at: 1645 3678 Size: 2033 Fragments arranged by size: 2033 594 368 362 147 128 20 17 9 NarI GG'CG_CC Cuts at: 0 2906 3678 Size: 2906 772 NcoI C'CATG_G Cuts at: 0 2060 2211 3678 Size: 2060 151 1467 Fragments arranged by size: 2060 1467 151 NdeI CA'TA_TG Cuts at: 0 1054 3678 Size: 1054 2624 PasI CC'CwG_GG Cuts at: 0 281 3678 Size: 281 3397 PfoI T'CCnGG_A Cuts at: 0 389 437 2743 3352 3413 3678 Size: 389 48 2306 609 61 265 Fragments arranged by size: 2306 609 389 265 61 48 PmlI CAC'GTG Cuts at: 0 340 3678 Size: 340 3338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2254 2286 3678 Size: 2254 32 1392 Fragments arranged by size: 2254 1392 32 PpuMI rG'GwC_Cy Cuts at: 0 2471 3678 Size: 2471 1207 PshAI GACnn'nnGTC Cuts at: 0 3373 3678 Size: 3373 305 PstI C_TGCA'G Cuts at: 0 1791 2929 3173 3678 Size: 1791 1138 244 505 Fragments arranged by size: 1791 1138 505 244 PvuII CAG'CTG Cuts at: 0 1132 1500 1645 3678 Size: 1132 368 145 2033 Fragments arranged by size: 2033 1132 368 145 SacI G_AGCT'C Cuts at: 0 2156 3678 Size: 2156 1522 SacII CC_GC'GG Cuts at: 0 10 3678 Size: 10 3668 SalI G'TCGA_C Cuts at: 0 2239 3678 Size: 2239 1439 SapI GCTCTTCn'nnn_ Cuts at: 0 437 1831 3678 Size: 437 1394 1847 Fragments arranged by size: 1847 1394 437 ScaI AGT'ACT Cuts at: 0 230 3678 Size: 230 3448 SexAI A'CCwGG_T Cuts at: 0 1407 3678 Size: 1407 2271 SfcI C'TryA_G Cuts at: 0 553 1029 1446 1787 2769 2925 3105 3169 Size: 553 476 417 341 982 156 180 64 Cuts at: 3169 3678 Size: 509 Fragments arranged by size: 982 553 509 476 417 341 180 156 64 SfoI GGC'GCC Cuts at: 0 2907 3678 Size: 2907 771 SgrAI Cr'CCGG_yG Cuts at: 0 162 2546 3678 Size: 162 2384 1132 Fragments arranged by size: 2384 1132 162 SmaI CCC'GGG Cuts at: 0 3463 3678 Size: 3463 215 SmlI C'TyrA_G Cuts at: 0 121 139 724 871 1858 2466 3678 Size: 121 18 585 147 987 608 1212 Fragments arranged by size: 1212 987 608 585 147 121 18 SspI AAT'ATT Cuts at: 0 963 3678 Size: 963 2715 StuI AGG'CCT Cuts at: 0 3605 3678 Size: 3605 73 StyI C'CwwG_G Cuts at: 0 217 651 798 857 974 1724 2060 2211 Size: 217 434 147 59 117 750 336 151 Cuts at: 2211 3005 3216 3311 3678 Size: 794 211 95 367 Fragments arranged by size: 794 750 434 367 336 217 211 151 147 117 95 59 SwaI ATTT'AAAT Cuts at: 0 1605 3678 Size: 1605 2073 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 55 2173 2632 3678 Size: 55 2118 459 1046 Fragments arranged by size: 2118 1046 459 55 TatI w'GTAC_w Cuts at: 0 228 3678 Size: 228 3450 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 185 217 2661 2693 3678 Size: 185 32 2444 32 985 Fragments arranged by size: 2444 985 185 32 32 Tth111I GACn'n_nGTC Cuts at: 0 183 2862 3678 Size: 183 2679 816 Fragments arranged by size: 2679 816 183 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 855 1221 1995 2287 3313 3432 3678 Size: 855 366 774 292 1026 119 246 Fragments arranged by size: 1026 855 774 366 292 246 119 XmaI C'CCGG_G Cuts at: 0 3461 3678 Size: 3461 217 XmnI GAAnn'nnTTC Cuts at: 0 719 3592 3678 Size: 719 2873 86 Fragments arranged by size: 2873 719 86 Enzymes that do cut: AccI Acc65I AcuI AflII AgeI AleI AlfI AloI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NarI NcoI NdeI PasI PfoI PmlI PpiI PpuMI PshAI PstI PvuII SacI SacII SalI SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflIII AhdI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BclI BfrBI BmgBI BmtI BsgI BsiWI BspHI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI EciI EcoRV FseI FspAI HindIII HpaI MfeI MluI MmeI NaeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PsiI PspOMI PspXI PsrI PvuI RsrII SanDI SbfI SfiI SgfI SnaBI SpeI SphI SrfI XbaI XhoI ZraI