################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbrPI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoRV FbaI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MluI MroI MvnI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 304 GAATTC [GA]^AATT[CT] AcyI 3349 GGCGCC G[GA]^CG[CT]C AflIII 1514 ACATGT A^C[GA][CT]GT Alw44I 2948 GTGCAC G^TGCAC ApaLI 2948 GTGCAC G^TGCAC ApoI 304 GAATTC [GA]^AATT[CT] Asp700I 1579 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 3214 GGATCC G^GATCC BbeI 3349 GGCGCC GGCGC^C BbvCI 637 CCTCAGC CCTCAGC(-5/-2) BglII 2392 AGATCT A^GATCT BlpI 2757 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2757 GCTCAGC GC^T[ACGT]AGC BsaHI 3349 GGCGCC G[GA]^CG[CT]C BsiEI 1913 CGGCCG CG[GA][CT]^CG BspHI 1862 TCATGA T^CATGA BspLU11I 1514 ACATGT A^CATGT BsrBI 2805 CCGCTC CCGCTC(-3/-3) BstAPI 3244 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3403 GGTGACC G^GT[ACGT]ACC BstPI 3403 GGTGACC G^GT[ACGT]ACC CelII 2757 GCTCAGC GC^T[ACGT]AGC DrdI 2547 GACTGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1913 CGGCCG C^GGCCG EclXI 1913 CGGCCG C^GGCCG Eco52I 1913 CGGCCG C^GGCCG EcoO65I 3403 GGTGACC G^GT[ACGT]ACC EcoRI 304 GAATTC G^AATTC EcoT22I 23 ATGCAT ATGCA^T Hin1I 3349 GGCGCC G[GA]^CG[CT]C HincII 1967 GTCAAC GT[CT]^[GA]AC HindII 1967 GTCAAC GT[CT]^[GA]AC KasI 3349 GGCGCC G^GCGCC MfeI 2939 CAATTG C^AATTG MunI 2939 CAATTG C^AATTG NarI 3349 GGCGCC GG^CGCC NcoI 884 CCATGG C^CATGG NheI 663 GCTAGC G^CTAGC NsiI 23 ATGCAT ATGCA^T PflMI 3420 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1862 TCATGA T^CATGA SacI 2074 GAGCTC GAGCT^C SbfI 3241 CCTGCAGG CCTGCA^GG SfiI 3370 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3349 GGCGCC GGC^GCC SmlI 2571 CTTGAG C^T[CT][GA]AG Sse8387I 3241 CCTGCAGG CCTGCA^GG Van91I 3420 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1579 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AocI 559 CCTCAGG CC^T[ACGT]AGG 1994 CCTCAGG BanI 750 GGCACC G^G[CT][GA]CC 3349 GGCGCC BanII 2074 GAGCTC G[GA]GC[CT]^C 2472 GAGCCC BbsI 735 GAAGAC GAAGAC(2/6) 3258 GAAGAC BglI 2682 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3371 GCCTCAATGGC BpuAI 735 GAAGAC GAAGAC(2/6) 3258 GAAGAC BsgI 1850 CTGCAC GTGCAG(16/14) 1902 GTGCAG BsrDI 75 CATTGC GCAATG(2/0) 2325 CATTGC Bsu36I 559 CCTCAGG CC^T[ACGT]AGG 1994 CCTCAGG CfoI 3337 GCGC GCG^C 3350 GCGC DraII 1643 AGGTCCC [GA]G^G[ACGT]CC[CT] 3297 GGGTCCC DsaI 884 CCATGG C^C[GA][CT]GG 2822 CCACGG EarI 313 GAAGAG CTCTTC(1/4) 691 GAAGAG Eco81I 559 CCTCAGG CC^T[ACGT]AGG 1994 CCTCAGG EcoO109I 1643 AGGTCCC [GA]G^G[ACGT]CC[CT] 3297 GGGTCCC HaeII 3336 AGCGCC [GA]GCGC^[CT] 3349 GGCGCC HhaI 3337 GCGC GCG^C 3350 GCGC HinP1I 3337 GCGC G^CGC 3350 GCGC Ksp632I 313 GAAGAG CTCTTC(1/4) 691 GAAGAG MslI 433 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2673 CACACCCATG PpuMI 1643 AGGTCCC [GA]G^G[AT]CC[CT] 3297 GGGTCCC PshAI 7 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2569 GACTTGAGTC SspI 489 AATATT AAT^ATT 3037 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII EaeI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII