(Circular) (Six-base) MAPSORT of: pFN21AE2565.seq Check: 5742 from: 1 to: 8161 pFN21AE2565 8161 bp With 182 enzymes: SgfI * March 23, 2015 11:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5971 278 Size: 53 83 186 936 4435 2468 Fragments arranged by size: 4435 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5504 1937 Size: 3567 4594 Acc65I G'GTAC_C Cuts at: 5482 5482 Size: 8161 AclI AA'CG_TT Cuts at: 1563 6288 6661 1563 Size: 4725 373 3063 Fragments arranged by size: 4725 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2759 2976 3975 4221 4820 5349 5367 6224 Size: 1127 217 999 246 599 529 18 857 Cuts at: 6224 7608 7876 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1127 999 857 599 529 268 246 217 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7964 AflIII A'CryG_T Cuts at: 1203 3519 7060 8037 1203 Size: 2316 3541 977 1327 Fragments arranged by size: 3541 2316 1327 977 AgeI A'CCGG_T Cuts at: 6992 6992 Size: 8161 AhdI GACnn_n'nnGTC Cuts at: 6890 6890 Size: 8161 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3870 1184 Size: 2686 5475 AlwNI CAG_nnn'CTG Cuts at: 2627 4382 4445 5345 5904 7476 2627 Size: 1755 63 900 559 1572 3312 Fragments arranged by size: 3312 1755 1572 900 559 63 ApaLI G'TGCA_C Cuts at: 4953 6218 7374 4953 Size: 1265 1156 5740 Fragments arranged by size: 5740 1265 1156 ApoI r'AATT_y Cuts at: 1493 2309 5470 5696 5848 7957 1493 Size: 816 3161 226 152 2109 1697 Fragments arranged by size: 3161 2109 1697 816 226 152 AseI AT'TA_AT Cuts at: 160 1032 6715 160 Size: 872 5683 1606 Fragments arranged by size: 5683 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8161 AvaI C'yCGr_G Cuts at: 1870 1943 5486 1870 Size: 73 3543 4545 Fragments arranged by size: 4545 3543 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2970 3003 2970 Size: 33 8128 BamHI G'GATC_C Cuts at: 5219 5491 5858 5219 Size: 272 367 7522 Fragments arranged by size: 7522 367 272 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2755 5354 5482 6937 618 Size: 325 217 627 968 2599 128 1455 1842 Fragments arranged by size: 2599 1842 1455 968 627 325 217 128 BanII G_rGCy'C Cuts at: 1391 4083 4481 5480 1391 Size: 2692 398 999 4072 Fragments arranged by size: 4072 2692 999 398 BbeI G_GCGC'C Cuts at: 5358 5358 Size: 8161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2747 5270 928 Size: 971 848 2523 3819 Fragments arranged by size: 3819 2523 971 848 BbvCI CC'TCA_GC Cuts at: 2643 2643 Size: 8161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6352 6386 1903 Size: 34 4415 34 3678 Fragments arranged by size: 4415 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6053 7263 6053 Size: 1210 6951 BclI T'GATC_A Cuts at: 1548 1548 Size: 8161 BfrBI ATG'CAT Cuts at: 2030 2030 Size: 8161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4693 5382 6772 Size: 107 122 71 631 605 3021 689 1390 Cuts at: 6772 136 Size: 1525 Fragments arranged by size: 3021 1525 1390 689 631 605 122 107 71 BglII A'GATC_T Cuts at: 4397 8156 4397 Size: 3759 4402 BlpI GC'TnA_GC Cuts at: 4763 5578 4763 Size: 815 7346 Bme1580I G_kGCm'C Cuts at: 1790 4957 6222 7378 1790 Size: 3167 1265 1156 2573 Fragments arranged by size: 3167 2573 1265 1156 BmgBI CAC'GTC Cuts at: 1318 4315 1318 Size: 2997 5164 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3419 5892 6850 Size: 416 53 83 399 24 2002 2473 958 Cuts at: 6850 442 Size: 1753 Fragments arranged by size: 2473 2002 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2672 1056 Size: 1616 6545 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3093 3171 3093 Size: 78 8083 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2031 2455 3558 3600 3702 4378 4872 5079 Size: 792 424 1103 42 102 676 494 207 Cuts at: 5079 6821 1239 Size: 1742 2579 Fragments arranged by size: 2579 1742 1103 792 676 494 424 207 102 42 Bpu10I CC'TnA_GC Cuts at: 2391 2643 3681 4179 2391 Size: 252 1038 498 6373 Fragments arranged by size: 6373 1038 498 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4597 6286 7151 7449 4597 Size: 1689 865 298 5309 Fragments arranged by size: 5309 1689 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2304 3989 5113 6824 882 Size: 617 805 1685 1124 1711 2219 Fragments arranged by size: 2219 1711 1685 1124 805 617 BsaAI yAC'GTr Cuts at: 493 1204 5952 493 Size: 711 4748 2702 Fragments arranged by size: 4748 2702 711 BsaBI GATnn'nnATC Cuts at: 8155 8155 Size: 8161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5355 5968 6350 6985 Size: 53 83 186 936 3822 613 382 635 Cuts at: 6985 275 Size: 1451 Fragments arranged by size: 3822 1451 936 635 613 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6594 6992 7266 7413 7824 6594 Size: 398 274 147 411 6931 Fragments arranged by size: 6931 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 2235 2265 2284 Size: 30 165 30 709 30 93 30 19 Cuts at: 2284 2314 3556 3586 4774 4804 5148 5178 5244 Size: 30 1242 30 1188 30 344 30 66 Cuts at: 5244 5274 1178 Size: 30 4065 Fragments arranged by size: 4065 1242 1188 709 344 165 93 66 30 30 30 30 30 30 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2631 3938 4869 5101 5172 5280 5333 1186 Size: 1445 1307 931 232 71 108 53 4014 Fragments arranged by size: 4014 1445 1307 931 232 108 71 53 BseYI C'CCAG_C Cuts at: 3034 3220 3652 4387 4661 4742 5312 7364 7860 Size: 186 432 735 274 81 570 2052 496 Cuts at: 7860 3034 Size: 3335 Fragments arranged by size: 3335 2052 735 570 496 432 274 186 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3840 3928 1163 Size: 573 2104 88 5396 Fragments arranged by size: 5396 2104 573 88 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3921 5611 6372 7400 1540 Size: 275 186 1920 1690 761 1028 2301 Fragments arranged by size: 2301 1920 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2207 4083 4415 4595 4957 6222 6307 7378 2207 Size: 1876 332 180 362 1265 85 1071 2990 Fragments arranged by size: 2990 1876 1265 1071 362 332 180 85 BsmI GAATG_Cn' Cuts at: 5672 5765 5672 Size: 93 8068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 4083 4415 4481 4595 4957 5480 Size: 399 417 1876 332 66 114 362 523 Cuts at: 5480 6222 6307 7378 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1876 1071 742 523 417 399 362 332 114 85 66 BspEI T'CCGG_A Cuts at: 7824 7824 Size: 8161 BspHI T'CATG_A Cuts at: 3867 6050 7780 3867 Size: 2183 1730 4248 Fragments arranged by size: 4248 2183 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4138 4540 4975 5254 5516 844 Size: 3294 402 435 279 262 3489 Fragments arranged by size: 3489 3294 435 402 279 262 BsrBI CCG'CTC Cuts at: 1394 1823 4812 6048 1394 Size: 429 2989 1236 3507 Fragments arranged by size: 3507 2989 1236 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2079 4329 6656 6830 65 Size: 1183 831 2250 2327 174 1396 Fragments arranged by size: 2327 2250 1396 1183 831 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3916 4937 5323 6805 6992 1700 Size: 110 2106 1021 386 1482 187 2869 Fragments arranged by size: 2869 2106 1482 1021 386 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8161 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8161 BssSI C'ACGA_G Cuts at: 6221 7233 6221 Size: 1012 7149 BstAPI GCAn_nnn'nTGC Cuts at: 794 5255 794 Size: 4461 3700 BstBI TT'CG_AA Cuts at: 6965 6965 Size: 8161 BstEII G'GTnAC_C Cuts at: 1184 5408 1184 Size: 4224 3937 BstXI CCAn_nnnn'nTGG Cuts at: 2794 3004 3112 3190 3232 3478 2794 Size: 210 108 78 42 246 7477 Fragments arranged by size: 7477 246 210 108 78 42 BstYI r'GATC_y Cuts at: 1972 4397 5219 5442 5491 5858 6243 6260 7712 Size: 2425 822 223 49 367 385 17 1452 Cuts at: 7712 7798 7810 8156 1972 Size: 86 12 346 1977 Fragments arranged by size: 2425 1977 1452 822 385 367 346 223 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2565 4000 2565 Size: 1435 6726 BtgI C'CryG_G Cuts at: 513 1065 2004 2889 4827 513 Size: 552 939 885 1938 3847 Fragments arranged by size: 3847 1938 939 885 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7422 Fragments arranged by size: 7422 683 56 BtsI GCAGTG_nn' Cuts at: 3356 4506 5677 6471 6491 3356 Size: 1150 1171 794 20 5026 Fragments arranged by size: 5026 1171 1150 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8161 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4183 4218 5134 5169 529 Size: 35 3619 35 916 35 3521 Fragments arranged by size: 3619 3521 916 35 35 35 DraI TTT'AAA Cuts at: 5465 5465 Size: 8161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4558 7168 809 Size: 507 3242 2610 1802 Fragments arranged by size: 3242 2610 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2360 2868 3342 Size: 54 1425 50 275 18 530 508 474 Cuts at: 3342 3468 3702 3918 4116 4734 4773 5325 5383 Size: 126 234 216 198 618 39 552 58 Cuts at: 5383 5608 6497 8126 8 Size: 225 889 1629 43 Fragments arranged by size: 1629 1425 889 618 552 530 508 474 275 234 225 216 198 126 58 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 3918 5608 1537 Size: 275 2106 1690 4090 Fragments arranged by size: 4090 2106 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2313 2691 6091 1353 Size: 960 378 3400 3423 Fragments arranged by size: 3423 3400 960 378 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6748 7122 7268 387 Size: 1064 138 5159 374 146 1280 Fragments arranged by size: 5159 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4081 4081 Size: 8161 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2455 2759 2976 3558 3600 3702 Size: 393 399 424 304 217 582 42 102 Cuts at: 3702 3975 4221 4378 4820 4872 5079 5349 5367 Size: 273 246 157 442 52 207 270 18 Cuts at: 5367 6224 6821 7608 7876 1239 Size: 857 597 787 268 1524 Fragments arranged by size: 1524 857 787 597 582 442 424 399 393 304 273 270 268 246 217 207 157 102 52 42 18 EcoNI CCTnn'n_nnAGG Cuts at: 2786 2912 2954 3638 3680 4285 4854 5154 5250 Size: 126 42 684 42 605 569 300 96 Cuts at: 5250 2786 Size: 5697 Fragments arranged by size: 5697 684 605 569 300 126 96 42 42 EcoO109I rG'GnC_Cy Cuts at: 3649 5303 3649 Size: 1654 6507 EcoRI G'AATT_C Cuts at: 2309 5470 5848 2309 Size: 3161 378 4622 Fragments arranged by size: 4622 3161 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2092 3928 5206 2092 Size: 1836 1278 5047 Fragments arranged by size: 5047 1836 1278 FspI TGC'GCA Cuts at: 6667 6667 Size: 8161 HaeII r_GCGC'y Cuts at: 1678 5345 5358 7308 1678 Size: 3667 13 1950 2531 Fragments arranged by size: 3667 2531 1950 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 2825 2857 3059 Size: 32 135 32 647 32 574 32 202 Cuts at: 3059 3091 3137 3169 3263 3295 3556 3588 3628 Size: 32 46 32 94 32 261 32 40 Cuts at: 3628 3660 5148 5161 5180 5193 6802 6834 6876 Size: 32 1488 13 19 13 1609 32 42 Cuts at: 6876 6908 1373 Size: 32 2626 Fragments arranged by size: 2626 1609 1488 647 574 261 202 135 94 46 42 40 32 32 32 32 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 3974 5505 5751 669 Size: 988 281 2036 1531 246 3079 Fragments arranged by size: 3079 2036 1531 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8161 HpaI GTT'AAC Cuts at: 5751 5751 Size: 8161 KasI G'GCGC_C Cuts at: 5354 5354 Size: 8161 KpnI G_GTAC'C Cuts at: 5486 5486 Size: 8161 MfeI C'AATT_G Cuts at: 4944 5760 4944 Size: 816 7345 MluI A'CGCG_T Cuts at: 8037 8037 Size: 8161 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 2327 2410 7275 7459 1305 Size: 805 217 83 4865 184 2007 Fragments arranged by size: 4865 2007 805 217 184 83 MscI TGG'CCA Cuts at: 10 64 1489 1832 2362 2870 3344 3704 4118 Size: 54 1425 343 530 508 474 360 414 Cuts at: 4118 4775 5385 10 Size: 657 610 2786 Fragments arranged by size: 2786 1425 657 610 530 508 474 414 360 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2442 4682 6119 6478 6637 518 Size: 648 1276 2240 1437 359 159 2042 Fragments arranged by size: 2240 2042 1437 1276 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2483 2555 3854 4253 4442 4571 4646 4892 Size: 877 72 1299 399 189 129 75 246 Cuts at: 4892 5577 6254 7402 7647 1606 Size: 685 677 1148 245 2120 Fragments arranged by size: 2120 1299 1148 877 685 677 399 246 245 189 129 75 72 NaeI GCC'GGC Cuts at: 3918 4939 5325 3918 Size: 1021 386 6754 Fragments arranged by size: 6754 1021 386 NarI GG'CG_CC Cuts at: 5355 5355 Size: 8161 NcoI C'CATG_G Cuts at: 513 1065 2004 2889 513 Size: 552 939 885 5785 Fragments arranged by size: 5785 939 885 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8161 NgoMIV G'CCGG_C Cuts at: 3916 4937 5323 3916 Size: 1021 386 6754 Fragments arranged by size: 6754 1021 386 NheI G'CTAG_C Cuts at: 1052 2668 1052 Size: 1616 6545 NotI GC'GGCC_GC Cuts at: 5608 5608 Size: 8161 NsiI A_TGCA'T Cuts at: 2032 2032 Size: 8161 NspI r_CATG'y Cuts at: 1132 1229 2881 3523 5054 5519 7064 1132 Size: 97 1652 642 1531 465 1545 2229 Fragments arranged by size: 2229 1652 1545 1531 642 465 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8161 PciI A'CATG_T Cuts at: 3519 7060 3519 Size: 3541 4620 PflMI CCAn_nnn'nTGG Cuts at: 1108 5431 1108 Size: 4323 3838 PfoI T'CCnGG_A Cuts at: 2154 5445 2154 Size: 3291 4870 PmeI GTTT'AAAC Cuts at: 5465 5465 Size: 8161 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6230 6262 7743 7775 6230 Size: 32 1481 32 6616 Fragments arranged by size: 6616 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3649 5303 3649 Size: 1654 6507 PshAI GACnn'nnGTC Cuts at: 1613 2016 4578 1613 Size: 403 2562 5196 Fragments arranged by size: 5196 2562 403 PsiI TTA'TAA Cuts at: 5731 5731 Size: 8161 PstI C_TGCA'G Cuts at: 830 3837 4537 4651 5251 5513 830 Size: 3007 700 114 600 262 3478 Fragments arranged by size: 3478 3007 700 600 262 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8161 PvuII CAG'CTG Cuts at: 2483 2555 3854 4253 4442 4571 4892 2483 Size: 72 1299 399 189 129 321 5752 Fragments arranged by size: 5752 1299 399 321 189 129 72 SacI G_AGCT'C Cuts at: 4083 4083 Size: 8161 SalI G'TCGA_C Cuts at: 1936 5503 1936 Size: 3567 4594 SanDI GG'GwC_CC Cuts at: 5303 5303 Size: 8161 SbfI CC_TGCA'GG Cuts at: 5251 5513 5251 Size: 262 7899 ScaI AGT'ACT Cuts at: 6409 8121 6409 Size: 1712 6449 SfcI C'TryA_G Cuts at: 826 1045 2292 3833 4533 4647 5005 5247 5509 Size: 219 1247 1541 700 114 358 242 262 Cuts at: 5509 6644 7325 7516 826 Size: 1135 681 191 1471 Fragments arranged by size: 1541 1471 1247 1135 700 681 358 262 242 219 191 114 SfiI GGCCn_nnn'nGGCC Cuts at: 5382 5382 Size: 8161 SfoI GGC'GCC Cuts at: 5356 5356 Size: 8161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8161 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8161 SmaI CCC'GGG Cuts at: 1872 5488 1872 Size: 3616 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4576 6265 7166 7428 820 Size: 197 926 2633 1689 901 262 1553 Fragments arranged by size: 2633 1689 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5952 493 Size: 5459 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8161 SphI G_CATG'C Cuts at: 1132 5519 1132 Size: 4387 3774 SspI AAT'ATT Cuts at: 5 52 2496 5044 6085 5 Size: 47 2444 2548 1041 2081 Fragments arranged by size: 2548 2444 2081 1041 47 StuI AGG'CCT Cuts at: 1517 2102 4030 4291 1517 Size: 585 1928 261 5387 Fragments arranged by size: 5387 1928 585 261 StyI C'CwwG_G Cuts at: 513 1065 2004 2673 2787 2871 2889 3039 3105 Size: 552 939 669 114 84 18 150 66 Cuts at: 3105 3183 3225 3387 3471 4815 5034 5211 5430 Size: 78 42 162 84 1344 219 177 219 Cuts at: 5430 5600 513 Size: 170 3074 Fragments arranged by size: 3074 1344 939 669 552 219 219 177 170 162 150 114 84 84 78 66 42 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2860 3136 3214 4276 4696 6188 6205 Size: 1255 1280 276 78 1062 420 1492 17 Cuts at: 6205 6358 6543 6988 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1492 1280 1255 1062 445 420 276 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6407 8119 96 Size: 274 80 33 51 1443 4430 1712 138 Fragments arranged by size: 4430 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2591 2623 4017 4049 5244 5276 8121 8153 2591 Size: 32 1394 32 1195 32 2845 32 2599 Fragments arranged by size: 2845 2599 1394 1195 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8161 XbaI T'CTAG_A Cuts at: 5497 5497 Size: 8161 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8161 XmaI C'CCGG_G Cuts at: 1870 5486 1870 Size: 3616 4545 XmnI GAAnn'nnTTC Cuts at: 3588 6290 3588 Size: 2702 5459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5969 276 Size: 53 83 186 936 4435 2468 Fragments arranged by size: 4435 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SrfI SwaI XcmI