################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV FauI FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AflIII 758 ACGTGT A^C[GA][CT]GT AgeI 719 ACCGGT A^CCGGT Asp700I 61 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1618 GAGCAC G[AT]GC[AT]^C AvaI 572 CTCGAG C^[CT]CG[GA]G AvaII 199 GGTCC G^G[AT]CC BbsI 222 GAAGAC GAAGAC(2/6) BciVI 1568 GTATCC GTATCC(6/5) BcnI 1595 CCGGG CC^[GC]GG BfaI 1027 CTAG C^TAG BglII 1779 AGATCT A^GATCT BmyI 1618 GAGCAC G[AGT]GC[ACT]^C BpuAI 222 GAAGAC GAAGAC(2/6) BsaBI 1563 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1943 GGTCTC GGTCTC(1/5) BsaWI 719 ACCGGT [AT]^CCGG[AT] BsgI 641 GTGCAG GTGCAG(16/14) BsiHKAI 1618 GAGCAC G[AT]GC[AT]^C BsmAI 1944 GTCTC GTCTC(1/5) BsmFI 1457 GTCCC GGGAC(10/14) BsoBI 572 CTCGAG C^[CT]CG[GA]G Bsp1286I 1618 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 896 TGTACA T^GTACA BspHI 432 TCATGA T^CATGA BsrFI 719 ACCGGT [GA]^CCGG[CT] BsrGI 896 TGTACA T^GTACA BstEII 394 GGTGACC G^GT[ACGT]ACC BstPI 394 GGTGACC G^GT[ACGT]ACC CfoI 752 GCGC GCG^C Cfr10I 719 ACCGGT [GA]^CCGG[CT] Cfr13I 199 GGTCC G^G[ACGT]CC DraI 1900 TTTAAA TTT^AAA EcoNI 1034 CCTTATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 394 GGTGACC G^GT[ACGT]ACC EcoT14I 1469 CCATGG C^C[AT][AT]GG HgaI 277 GACGC GACGC(5/10) HhaI 752 GCGC GCG^C HinP1I 752 GCGC G^CGC HincII 1657 GTTGAC GT[CT]^[GA]AC HindII 1657 GTTGAC GT[CT]^[GA]AC MaeI 1027 CTAG C^TAG MamI 1563 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 1595 CCGGG CC^[GC]GG NcoI 1469 CCATGG C^CATGG NdeI 802 CATATG CA^TATG NlaIV 1667 GGTTCC GG[ACGT]^[ACGT]CC PaeR7I 572 CTCGAG C^TCGAG PinAI 719 ACCGGT A^CCGGT RcaI 432 TCATGA T^CATGA SapI 266 GAAGAGC GCTCTTC(1/4) Sau96I 199 GGTCC G^G[ACGT]CC SmlI 572 CTCGAG C^T[CT][GA]AG SspBI 896 TGTACA T^GTACA StyI 1469 CCATGG C^C[AT][AT]GG SwaI 1899 ATTTAAAT ATTT^AAAT XhoI 572 CTCGAG C^TCGAG XmnI 61 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BbrPI 757 CACGTG CAC^GTG 1621 CACGTG BbvCI 1368 CCTCAGC CCTCAGC(-5/-2) 1056 GCTGAGG BsaAI 757 CACGTG [CT]AC^GT[GA] 1621 CACGTG BsmI 1552 GAATGC GAATGC(1/-1) 422 GCATTC BstYI 466 GGATCT [GA]^GATC[CT] 1779 AGATCT DsaI 767 CCACGG C^C[GA][CT]GG 1469 CCATGG EcoT22I 1063 ATGCAT ATGCA^T 1217 ATGCAT HapII 720 CCGG C^CGG 1595 CCGG HpaII 720 CCGG C^CGG 1595 CCGG MflI 466 GGATCT [GA]^GATC[CT] 1779 AGATCT MspI 720 CCGG C^CGG 1595 CCGG NsiI 1063 ATGCAT ATGCA^T 1217 ATGCAT PmaCI 757 CACGTG CAC^GTG 1621 CACGTG PmlI 757 CACGTG CAC^GTG 1621 CACGTG SphI 409 GCATGC GCATG^C 1818 GCATGC SspI 600 AATATT AAT^ATT 649 AATATT StuI 1912 AGGCCT AGG^CCT 1949 AGGCCT XcmI 300 CCAGGTGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2064 CCACGTTCCTCATGG XhoII 466 GGATCT [GA]^GATC[CT] 1779 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BpmI BsaJI BseRI BsiYI BslI BsrDI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NspI PleI PvuII RsaI Sau3AI ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I