(Linear) (Six-base) MAPSORT of: KIEE5738.seq Check: 781 from: 1 to: 2109 KIEE5738.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 829 1922 2109 Size: 829 1093 187 Fragments arranged by size: 1093 829 187 AflIII A'CryG_T Cuts at: 0 758 2109 Size: 758 1351 AgeI A'CCGG_T Cuts at: 0 719 2109 Size: 719 1390 AleI CACnn'nnGTG Cuts at: 0 1084 2109 Size: 1084 1025 ApoI r'AATT_y Cuts at: 0 856 1047 1235 1858 2109 Size: 856 191 188 623 251 Fragments arranged by size: 856 623 251 191 188 AvaI C'yCGr_G Cuts at: 0 572 2109 Size: 572 1537 BbsI GAAGACnn'nnnn_ Cuts at: 0 229 2109 Size: 229 1880 BbvCI CC'TCA_GC Cuts at: 0 1057 1369 2109 Size: 1057 312 740 Fragments arranged by size: 1057 740 312 BciVI GTATCCnnnnn_n' Cuts at: 0 1579 2109 Size: 1579 530 BfrBI ATG'CAT Cuts at: 0 1065 1219 2109 Size: 1065 154 890 Fragments arranged by size: 1065 890 154 BglII A'GATC_T Cuts at: 0 1779 2109 Size: 1779 330 BmrI ACTGGGnnnn_n' Cuts at: 0 42 1318 1568 2109 Size: 42 1276 250 541 Fragments arranged by size: 1276 541 250 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1791 2109 Size: 1791 318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1529 1661 1938 2109 Size: 1529 132 277 171 Fragments arranged by size: 1529 277 171 132 Bpu10I CC'TnA_GC Cuts at: 0 1057 1369 2109 Size: 1057 312 740 Fragments arranged by size: 1057 740 312 BsaI GGTCTCn'nnnn_ Cuts at: 0 1949 2109 Size: 1949 160 BsaAI yAC'GTr Cuts at: 0 759 1623 2109 Size: 759 864 486 Fragments arranged by size: 864 759 486 BsaBI GATnn'nnATC Cuts at: 0 1567 2109 Size: 1567 542 BsaWI w'CCGG_w Cuts at: 0 719 2109 Size: 719 1390 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 91 1244 2029 2109 Size: 91 1153 785 80 Fragments arranged by size: 1153 785 91 80 BseYI C'CCAG_C Cuts at: 0 697 985 2109 Size: 697 288 1124 Fragments arranged by size: 1124 697 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 662 2109 Size: 662 1447 BsiHKAI G_wGCw'C Cuts at: 0 1622 2109 Size: 1622 487 BsmI GAATG_Cn' Cuts at: 0 422 1558 2109 Size: 422 1136 551 Fragments arranged by size: 1136 551 422 Bsp1286I G_dGCh'C Cuts at: 0 1622 2109 Size: 1622 487 BspHI T'CATG_A Cuts at: 0 432 2109 Size: 432 1677 BsrDI GCAATG_nn' Cuts at: 0 287 747 980 1315 2109 Size: 287 460 233 335 794 Fragments arranged by size: 794 460 335 287 233 BsrFI r'CCGG_y Cuts at: 0 719 2109 Size: 719 1390 BsrGI T'GTAC_A Cuts at: 0 896 2109 Size: 896 1213 BstEII G'GTnAC_C Cuts at: 0 394 2109 Size: 394 1715 BstYI r'GATC_y Cuts at: 0 466 1779 2109 Size: 466 1313 330 Fragments arranged by size: 1313 466 330 BtgI C'CryG_G Cuts at: 0 767 1469 2109 Size: 767 702 640 Fragments arranged by size: 767 702 640 BtsI GCAGTG_nn' Cuts at: 0 278 420 872 1157 1974 2109 Size: 278 142 452 285 817 135 Fragments arranged by size: 817 452 285 278 142 135 DraI TTT'AAA Cuts at: 0 1902 2109 Size: 1902 207 EarI CTCTTCn'nnn_ Cuts at: 0 261 805 1475 2006 2109 Size: 261 544 670 531 103 Fragments arranged by size: 670 544 531 261 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 829 1529 1661 1922 1938 2109 Size: 829 700 132 261 16 171 Fragments arranged by size: 829 700 261 171 132 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 1038 2109 Size: 1038 1071 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1862 2109 Size: 1862 247 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 555 587 1004 1036 1429 1461 1572 1604 Size: 555 32 417 32 393 32 111 32 Cuts at: 1604 2109 Size: 505 Fragments arranged by size: 555 505 417 393 111 32 32 32 32 HincII GTy'rAC Cuts at: 0 1659 2109 Size: 1659 450 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 102 2109 Size: 102 2007 MslI CAynn'nnrTG Cuts at: 0 509 935 1070 1081 1084 1561 2109 Size: 509 426 135 11 3 477 548 Fragments arranged by size: 548 509 477 426 135 11 3 MspA1I CmG'CkG Cuts at: 0 1164 1549 1698 2109 Size: 1164 385 149 411 Fragments arranged by size: 1164 411 385 149 NcoI C'CATG_G Cuts at: 0 1469 2109 Size: 1469 640 NdeI CA'TA_TG Cuts at: 0 803 2109 Size: 803 1306 NsiI A_TGCA'T Cuts at: 0 1067 1221 2109 Size: 1067 154 888 Fragments arranged by size: 1067 888 154 NspI r_CATG'y Cuts at: 0 413 892 1822 2109 Size: 413 479 930 287 Fragments arranged by size: 930 479 413 287 PasI CC'CwG_GG Cuts at: 0 33 1532 2109 Size: 33 1499 577 Fragments arranged by size: 1499 577 33 PmlI CAC'GTG Cuts at: 0 759 1623 2109 Size: 759 864 486 Fragments arranged by size: 864 759 486 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 728 760 2109 Size: 728 32 1349 Fragments arranged by size: 1349 728 32 PvuII CAG'CTG Cuts at: 0 1164 1549 1698 2109 Size: 1164 385 149 411 Fragments arranged by size: 1164 411 385 149 SapI GCTCTTCn'nnn_ Cuts at: 0 261 2109 Size: 261 1848 SmlI C'TyrA_G Cuts at: 0 572 2109 Size: 572 1537 SphI G_CATG'C Cuts at: 0 413 1822 2109 Size: 413 1409 287 Fragments arranged by size: 1409 413 287 SspI AAT'ATT Cuts at: 0 602 651 2109 Size: 602 49 1458 Fragments arranged by size: 1458 602 49 StuI AGG'CCT Cuts at: 0 1914 1951 2109 Size: 1914 37 158 Fragments arranged by size: 1914 158 37 StyI C'CwwG_G Cuts at: 0 1469 2109 Size: 1469 640 SwaI ATTT'AAAT Cuts at: 0 1902 2109 Size: 1902 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 25 452 1448 2109 Size: 25 427 996 661 Fragments arranged by size: 996 661 427 25 TatI w'GTAC_w Cuts at: 0 896 2109 Size: 896 1213 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1136 1168 2109 Size: 1136 32 941 Fragments arranged by size: 1136 941 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 307 2071 2109 Size: 307 1764 38 Fragments arranged by size: 1764 307 38 XhoI C'TCGA_G Cuts at: 0 572 2109 Size: 572 1537 XmnI GAAnn'nnTTC Cuts at: 0 65 2109 Size: 65 2044 Enzymes that do cut: AcuI AflIII AgeI AleI ApoI AvaI BbsI BbvCI BciVI BfrBI BglII BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BstEII BstYI BtgI BtsI DraI EarI Eco57MI EcoNI FalI Hin4I HincII MmeI MslI MspA1I NcoI NdeI NsiI NspI PasI PmlI PpiI PvuII SapI SmlI SphI SspI StuI StyI SwaI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BcgI BclI BglI BlpI Bme1580I BmgBI BmtI BpuEI BsaHI BsaXI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI PacI PciI PflMI PfoI PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI Tth111I XbaI XmaI ZraI