(Linear) (Six-base) MAPSORT of: KIEE6755.seq Check: 1838 from: 1 to: 1659 KIEE6755.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 1439 1659 Size: 1439 220 AclI AA'CG_TT Cuts at: 0 1333 1659 Size: 1333 326 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 1139 1262 1659 Size: 556 583 123 397 Fragments arranged by size: 583 556 397 123 AflIII A'CryG_T Cuts at: 0 545 648 1659 Size: 545 103 1011 Fragments arranged by size: 1011 545 103 AleI CACnn'nnGTG Cuts at: 0 607 1659 Size: 607 1052 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 71 103 1659 Size: 71 32 1556 Fragments arranged by size: 1556 71 32 ApaI G_GGCC'C Cuts at: 0 674 1327 1659 Size: 674 653 332 Fragments arranged by size: 674 653 332 ApoI r'AATT_y Cuts at: 0 858 883 962 1103 1659 Size: 858 25 79 141 556 Fragments arranged by size: 858 556 141 79 25 BamHI G'GATC_C Cuts at: 0 1446 1659 Size: 1446 213 BanI G'GyrC_C Cuts at: 0 69 1659 Size: 69 1590 BanII G_rGCy'C Cuts at: 0 9 674 1256 1327 1535 1659 Size: 9 665 582 71 208 124 Fragments arranged by size: 665 582 208 124 71 9 BbeI G_GCGC'C Cuts at: 0 73 1659 Size: 73 1586 BbsI GAAGACnn'nnnn_ Cuts at: 0 175 503 1540 1659 Size: 175 328 1037 119 Fragments arranged by size: 1037 328 175 119 BbvCI CC'TCA_GC Cuts at: 0 1025 1162 1355 1659 Size: 1025 137 193 304 Fragments arranged by size: 1025 304 193 137 BciVI GTATCCnnnnn_n' Cuts at: 0 1017 1659 Size: 1017 642 BlpI GC'TnA_GC Cuts at: 0 686 1659 Size: 686 973 Bme1580I G_kGCm'C Cuts at: 0 674 1327 1659 Size: 674 653 332 Fragments arranged by size: 674 653 332 BmrI ACTGGGnnnn_n' Cuts at: 0 579 997 1452 1659 Size: 579 418 455 207 Fragments arranged by size: 579 455 418 207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1067 1659 Size: 1067 592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 455 643 1562 1659 Size: 455 188 919 97 Fragments arranged by size: 919 455 188 97 Bpu10I CC'TnA_GC Cuts at: 0 1025 1162 1355 1659 Size: 1025 137 193 304 Fragments arranged by size: 1025 304 193 137 BsaAI yAC'GTr Cuts at: 0 651 1659 Size: 651 1008 BsaHI Gr'CG_yC Cuts at: 0 70 1659 Size: 70 1589 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 182 212 1258 1288 1659 Size: 182 30 1046 30 371 Fragments arranged by size: 1046 371 182 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1078 1282 1417 1555 1582 1659 Size: 1078 204 135 138 27 77 Fragments arranged by size: 1078 204 138 135 77 27 BseYI C'CCAG_C Cuts at: 0 852 1030 1148 1659 Size: 852 178 118 511 Fragments arranged by size: 852 511 178 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1659 Size: 232 1427 BsiEI CG_ry'CG Cuts at: 0 15 31 1659 Size: 15 16 1628 Fragments arranged by size: 1628 16 15 BsiHKAI G_wGCw'C Cuts at: 0 614 687 1256 1391 1659 Size: 614 73 569 135 268 Fragments arranged by size: 614 569 268 135 73 BsmI GAATG_Cn' Cuts at: 0 262 1659 Size: 262 1397 Bsp1286I G_dGCh'C Cuts at: 0 9 614 674 687 1256 1327 1391 1535 Size: 9 605 60 13 569 71 64 144 Cuts at: 1535 1659 Size: 124 Fragments arranged by size: 605 569 144 124 71 64 60 13 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 126 1659 Size: 126 1533 BsrFI r'CCGG_y Cuts at: 0 1572 1659 Size: 1572 87 BstXI CCAn_nnnn'nTGG Cuts at: 0 479 1659 Size: 479 1180 BstYI r'GATC_y Cuts at: 0 1446 1659 Size: 1446 213 Bsu36I CC'TnA_GG Cuts at: 0 1042 1052 1659 Size: 1042 10 607 Fragments arranged by size: 1042 607 10 BtsI GCAGTG_nn' Cuts at: 0 709 1005 1659 Size: 709 296 654 Fragments arranged by size: 709 654 296 EaeI y'GGCC_r Cuts at: 0 12 28 1659 Size: 12 16 1631 Fragments arranged by size: 1631 16 12 EagI C'GGCC_G Cuts at: 0 12 28 1659 Size: 12 16 1631 Fragments arranged by size: 1631 16 12 EarI CTCTTCn'nnn_ Cuts at: 0 478 1055 1238 1412 1659 Size: 478 577 183 174 247 Fragments arranged by size: 577 478 247 183 174 EcoICRI GAG'CTC Cuts at: 0 1254 1659 Size: 1254 405 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 455 556 643 1139 1262 1562 1659 Size: 455 101 87 496 123 300 97 Fragments arranged by size: 496 455 300 123 101 97 87 EcoNI CCTnn'n_nnAGG Cuts at: 0 1381 1659 Size: 1381 278 EcoO109I rG'GnC_Cy Cuts at: 0 670 671 1046 1237 1659 Size: 670 1 375 191 422 Fragments arranged by size: 670 422 375 191 1 HaeII r_GCGC'y Cuts at: 0 73 1659 Size: 73 1586 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 430 462 715 747 1258 1290 1659 Size: 430 32 253 32 511 32 369 Fragments arranged by size: 511 430 369 253 32 32 32 HincII GTy'rAC Cuts at: 0 1440 1659 Size: 1440 219 KasI G'GCGC_C Cuts at: 0 69 1659 Size: 69 1590 MslI CAynn'nnrTG Cuts at: 0 145 607 1659 Size: 145 462 1052 Fragments arranged by size: 1052 462 145 MspA1I CmG'CkG Cuts at: 0 33 39 48 1034 1659 Size: 33 6 9 986 625 Fragments arranged by size: 986 625 33 9 6 NaeI GCC'GGC Cuts at: 0 1574 1659 Size: 1574 85 NarI GG'CG_CC Cuts at: 0 70 1659 Size: 70 1589 NgoMIV G'CCGG_C Cuts at: 0 1572 1659 Size: 1572 87 NotI GC'GGCC_GC Cuts at: 0 28 1659 Size: 28 1631 PasI CC'CwG_GG Cuts at: 0 667 1659 Size: 667 992 PflMI CCAn_nnn'nTGG Cuts at: 0 606 1659 Size: 606 1053 PmlI CAC'GTG Cuts at: 0 651 1659 Size: 651 1008 PshAI GACnn'nnGTC Cuts at: 0 1435 1659 Size: 1435 224 PspOMI G'GGCC_C Cuts at: 0 670 1323 1659 Size: 670 653 336 Fragments arranged by size: 670 653 336 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 622 654 1659 Size: 622 32 1005 Fragments arranged by size: 1005 622 32 SacI G_AGCT'C Cuts at: 0 1256 1659 Size: 1256 403 SalI G'TCGA_C Cuts at: 0 1438 1659 Size: 1438 221 SapI GCTCTTCn'nnn_ Cuts at: 0 1238 1412 1659 Size: 1238 174 247 Fragments arranged by size: 1238 247 174 ScaI AGT'ACT Cuts at: 0 1342 1659 Size: 1342 317 SfcI C'TryA_G Cuts at: 0 434 1659 Size: 434 1225 SfoI GGC'GCC Cuts at: 0 71 1659 Size: 71 1588 StyI C'CwwG_G Cuts at: 0 73 1659 Size: 73 1586 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 866 1162 1659 Size: 866 296 497 Fragments arranged by size: 866 497 296 TatI w'GTAC_w Cuts at: 0 1340 1659 Size: 1340 319 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 640 672 1659 Size: 640 32 987 Fragments arranged by size: 987 640 32 XbaI T'CTAG_A Cuts at: 0 1555 1659 Size: 1555 104 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 603 1659 Size: 603 1056 XmnI GAAnn'nnTTC Cuts at: 0 500 1659 Size: 500 1159 Enzymes that do cut: AccI AclI AcuI AflIII AleI AloI ApaI ApoI BamHI BanI BanII BbeI BbsI BbvCI BciVI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstXI BstYI Bsu36I BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI PmlI PshAI PspOMI PsrI SacI SalI SapI ScaI SfcI SfoI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BcgI BclI BfrBI BglI BglII BmgBI BmtI BpuEI BsaI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII SacII SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI ZraI