(Circular) (Six-base) MAPSORT of: pFN21AE2400.seq Check: 2024 from: 1 to: 6802 pFN21AE2400 6802 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2923 4612 278 Size: 53 83 186 936 1387 1689 2468 Fragments arranged by size: 2468 1689 1387 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4145 1937 Size: 2208 4594 Acc65I G'GTAC_C Cuts at: 4123 4123 Size: 6802 AclI AA'CG_TT Cuts at: 1563 4929 5302 1563 Size: 3366 373 3063 Fragments arranged by size: 3366 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 3004 3780 3860 4865 6249 6517 1632 Size: 437 935 776 80 1005 1384 268 1917 Fragments arranged by size: 1917 1384 1005 935 776 437 268 80 AfeI AGC'GCT Cuts at: 2336 2336 Size: 6802 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6605 AflIII A'CryG_T Cuts at: 1203 3209 5701 6678 1203 Size: 2006 2492 977 1327 Fragments arranged by size: 2492 2006 1327 977 AgeI A'CCGG_T Cuts at: 5633 5633 Size: 6802 AhdI GACnn_n'nnGTC Cuts at: 5531 5531 Size: 6802 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6802 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3903 1184 Size: 2719 4083 AlwNI CAG_nnn'CTG Cuts at: 2092 3062 4024 4545 6117 2092 Size: 970 962 521 1572 2777 Fragments arranged by size: 2777 1572 970 962 521 ApaLI G'TGCA_C Cuts at: 4859 6015 4859 Size: 1156 5646 ApoI r'AATT_y Cuts at: 1493 2255 2354 2711 3112 3636 4111 4337 4489 Size: 762 99 357 401 524 475 226 152 Cuts at: 4489 6598 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 762 524 475 401 357 226 152 99 AseI AT'TA_AT Cuts at: 160 1032 3450 5356 160 Size: 872 2418 1906 1606 Fragments arranged by size: 2418 1906 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 AvaI C'yCGr_G Cuts at: 1870 1943 2588 4127 1870 Size: 73 645 1539 4545 Fragments arranged by size: 4545 1539 645 73 BamHI G'GATC_C Cuts at: 4132 4499 4132 Size: 367 6435 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3967 4123 5578 618 Size: 325 217 627 2180 156 1455 1842 Fragments arranged by size: 2180 1842 1455 627 325 217 156 BanII G_rGCy'C Cuts at: 1391 4121 1391 Size: 2730 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3347 928 Size: 971 1448 4383 Fragments arranged by size: 4383 1448 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 4993 5027 1903 Size: 34 2012 34 1010 34 3678 Fragments arranged by size: 3678 2012 1010 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3517 3922 4694 5904 3517 Size: 405 772 1210 4415 Fragments arranged by size: 4415 1210 772 405 BclI T'GATC_A Cuts at: 1548 2662 3825 3831 1548 Size: 1114 1163 6 4519 Fragments arranged by size: 4519 1163 1114 6 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5413 136 Size: 107 122 71 631 605 3741 1525 Fragments arranged by size: 3741 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6797 6797 Size: 6802 BlpI GC'TnA_GC Cuts at: 4219 4219 Size: 6802 Bme1580I G_kGCm'C Cuts at: 1790 3727 3972 4863 6019 1790 Size: 1937 245 891 1156 2573 Fragments arranged by size: 2573 1937 1156 891 245 BmgBI CAC'GTC Cuts at: 1318 3212 1318 Size: 1894 4908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2304 4533 5491 Size: 416 53 83 399 24 887 2229 958 Cuts at: 5491 442 Size: 1753 Fragments arranged by size: 2229 1753 958 887 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2114 2969 2981 5462 1239 Size: 845 30 855 12 2481 2579 Fragments arranged by size: 2579 2481 855 845 30 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3242 4927 5792 6090 3242 Size: 1685 865 298 3954 Fragments arranged by size: 3954 1685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2060 3929 5465 882 Size: 617 561 1869 1536 2219 Fragments arranged by size: 2219 1869 1536 617 561 BsaAI yAC'GTr Cuts at: 493 1204 4593 493 Size: 711 3389 2702 Fragments arranged by size: 3389 2702 711 BsaBI GATnn'nnATC Cuts at: 3830 6796 3830 Size: 2966 3836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2920 4609 4991 5626 Size: 53 83 186 936 1387 1689 382 635 Cuts at: 5626 275 Size: 1451 Fragments arranged by size: 1689 1451 1387 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5235 5633 5907 6054 6465 5235 Size: 398 274 147 411 5572 Fragments arranged by size: 5572 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6577 Fragments arranged by size: 6577 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3239 3390 1186 Size: 2053 151 4598 Fragments arranged by size: 4598 2053 151 BseYI C'CCAG_C Cuts at: 2331 6005 6501 2331 Size: 3674 496 2632 Fragments arranged by size: 3674 2632 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6229 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4252 5013 6041 1540 Size: 275 186 2251 761 1028 2301 Fragments arranged by size: 2301 2251 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4863 4948 6019 4863 Size: 85 1071 5646 Fragments arranged by size: 5646 1071 85 BsmI GAATG_Cn' Cuts at: 3874 3996 4313 4406 3874 Size: 122 317 93 6270 Fragments arranged by size: 6270 317 122 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2044 2044 Size: 6802 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3727 3972 4121 4863 4948 6019 1391 Size: 399 1937 245 149 742 85 1071 2174 Fragments arranged by size: 2174 1937 1071 742 399 245 149 85 BspEI T'CCGG_A Cuts at: 6465 6465 Size: 6802 BspHI T'CATG_A Cuts at: 3006 3828 3837 4691 6421 3006 Size: 822 9 854 1730 3387 Fragments arranged by size: 3387 1730 854 822 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 4157 844 Size: 1957 1356 3489 Fragments arranged by size: 3489 1957 1356 BsrBI CCG'CTC Cuts at: 1394 1823 4689 1394 Size: 429 2866 3507 Fragments arranged by size: 3507 2866 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2146 5297 5471 65 Size: 1183 898 3151 174 1396 Fragments arranged by size: 3151 1396 1183 898 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3156 5446 5633 1700 Size: 110 1346 2290 187 2869 Fragments arranged by size: 2869 2290 1346 187 110 BsrGI T'GTAC_A Cuts at: 96 2263 96 Size: 2167 4635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6802 BssSI C'ACGA_G Cuts at: 2775 4862 5874 2775 Size: 2087 1012 3703 Fragments arranged by size: 3703 2087 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2793 794 Size: 1999 4803 BstBI TT'CG_AA Cuts at: 2433 5606 2433 Size: 3173 3629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6802 BstYI r'GATC_y Cuts at: 1972 3144 3546 4132 4499 4884 4901 6353 6439 Size: 1172 402 586 367 385 17 1452 86 Cuts at: 6439 6451 6797 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1172 586 402 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4070 513 Size: 552 3005 3245 Fragments arranged by size: 3245 3005 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6063 Fragments arranged by size: 6063 683 56 BtsI GCAGTG_nn' Cuts at: 2826 4318 5112 5132 2826 Size: 1492 794 20 4496 Fragments arranged by size: 4496 1492 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6802 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6767 DraI TTT'AAA Cuts at: 2572 4106 2572 Size: 1534 5268 DraIII CAC_nnn'GTG Cuts at: 2721 3979 2721 Size: 1258 5544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5809 809 Size: 507 4493 1802 Fragments arranged by size: 4493 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3474 4249 5138 Size: 54 1425 50 275 18 1644 775 889 Cuts at: 5138 6767 8 Size: 1629 43 Fragments arranged by size: 1644 1629 1425 889 775 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4249 1537 Size: 275 2437 4090 Fragments arranged by size: 4090 2437 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3079 3879 4732 1353 Size: 1726 800 853 3423 Fragments arranged by size: 3423 1726 853 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5389 5763 5909 387 Size: 1064 138 3800 374 146 1280 Fragments arranged by size: 3800 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2084 2114 2969 2981 3004 3780 Size: 393 437 15 30 855 12 23 776 Cuts at: 3780 3860 4865 5462 6249 6517 1239 Size: 80 1005 597 787 268 1524 Fragments arranged by size: 1524 1005 855 787 776 597 437 393 268 80 30 23 15 12 EcoRI G'AATT_C Cuts at: 4111 4489 4111 Size: 378 6424 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3034 3785 3034 Size: 751 6051 FspI TGC'GCA Cuts at: 5308 5308 Size: 6802 HaeII r_GCGC'y Cuts at: 1678 2338 5949 1678 Size: 660 3611 2531 Fragments arranged by size: 3611 2531 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2075 2420 2452 3072 Size: 32 135 32 471 32 345 32 620 Cuts at: 3072 3104 3533 3565 5443 5475 5517 5549 1373 Size: 32 429 32 1878 32 42 32 2626 Fragments arranged by size: 2626 1878 620 471 429 345 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3091 4146 4392 669 Size: 988 281 1153 1055 246 3079 Fragments arranged by size: 3079 1153 1055 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6802 HpaI GTT'AAC Cuts at: 4392 4392 Size: 6802 KpnI G_GTAC'C Cuts at: 4127 4127 Size: 6802 MfeI C'AATT_G Cuts at: 2457 2543 4401 2457 Size: 86 1858 4858 Fragments arranged by size: 4858 1858 86 MluI A'CGCG_T Cuts at: 6678 6678 Size: 6802 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2563 5916 6100 1305 Size: 1258 3353 184 2007 Fragments arranged by size: 3353 2007 1258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3476 10 Size: 54 1425 343 1644 3336 Fragments arranged by size: 3336 1644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3198 3450 3852 4760 5119 5278 518 Size: 648 2032 252 402 908 359 159 2042 Fragments arranged by size: 2042 2032 908 648 402 359 252 159 MspA1I CmG'CkG Cuts at: 1606 4218 4895 6043 6288 1606 Size: 2612 677 1148 245 2120 Fragments arranged by size: 2612 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3158 3158 Size: 6802 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6250 NdeI CA'TA_TG Cuts at: 387 387 Size: 6802 NgoMIV G'CCGG_C Cuts at: 3156 3156 Size: 6802 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6802 NotI GC'GGCC_GC Cuts at: 4249 4249 Size: 6802 NspI r_CATG'y Cuts at: 1132 1229 4160 5705 1132 Size: 97 2931 1545 2229 Fragments arranged by size: 2931 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6802 PciI A'CATG_T Cuts at: 5701 5701 Size: 6802 PflMI CCAn_nnn'nTGG Cuts at: 1108 2545 3979 1108 Size: 1437 1434 3931 Fragments arranged by size: 3931 1437 1434 PfoI T'CCnGG_A Cuts at: 2061 3270 2061 Size: 1209 5593 PmeI GTTT'AAAC Cuts at: 4106 4106 Size: 6802 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4871 4903 6384 6416 4871 Size: 32 1481 32 5257 Fragments arranged by size: 5257 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6802 PsiI TTA'TAA Cuts at: 4372 4372 Size: 6802 PstI C_TGCA'G Cuts at: 830 2953 4154 830 Size: 2123 1201 3478 Fragments arranged by size: 3478 2123 1201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6802 SalI G'TCGA_C Cuts at: 1936 4144 1936 Size: 2208 4594 SapI GCTCTTCn'nnn_ Cuts at: 3879 3879 Size: 6802 SbfI CC_TGCA'GG Cuts at: 4154 4154 Size: 6802 ScaI AGT'ACT Cuts at: 3618 5050 6762 3618 Size: 1432 1712 3658 Fragments arranged by size: 3658 1712 1432 SfcI C'TryA_G Cuts at: 826 1045 2680 2949 4095 4150 5285 5966 6157 Size: 219 1635 269 1146 55 1135 681 191 Cuts at: 6157 826 Size: 1471 Fragments arranged by size: 1635 1471 1146 1135 681 269 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6802 SmaI CCC'GGG Cuts at: 1872 2590 4129 1872 Size: 718 1539 4545 Fragments arranged by size: 4545 1539 718 SmlI C'TyrA_G Cuts at: 820 1017 1943 3221 4906 5807 6069 820 Size: 197 926 1278 1685 901 262 1553 Fragments arranged by size: 1685 1553 1278 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4593 493 Size: 4100 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6802 SphI G_CATG'C Cuts at: 1132 4160 1132 Size: 3028 3774 SspI AAT'ATT Cuts at: 5 52 3045 4726 5 Size: 47 2993 1681 2081 Fragments arranged by size: 2993 2081 1681 47 StuI AGG'CCT Cuts at: 1517 2082 1517 Size: 565 6237 StyI C'CwwG_G Cuts at: 513 1065 2269 2520 4241 513 Size: 552 1204 251 1721 3074 Fragments arranged by size: 3074 1721 1204 552 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 4829 4846 4999 5184 5629 325 Size: 1255 1527 1722 17 153 185 445 1498 Fragments arranged by size: 1722 1527 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2097 2263 3479 Size: 274 80 33 51 1443 120 166 1216 Cuts at: 3479 3616 5048 6760 96 Size: 137 1432 1712 138 Fragments arranged by size: 1712 1443 1432 1216 274 166 138 137 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2958 2990 6762 6794 2958 Size: 32 3772 32 2966 Fragments arranged by size: 3772 2966 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6802 XbaI T'CTAG_A Cuts at: 4138 4138 Size: 6802 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6802 XmaI C'CCGG_G Cuts at: 1870 2588 4127 1870 Size: 718 1539 4545 Fragments arranged by size: 4545 1539 718 XmnI GAAnn'nnTTC Cuts at: 2220 4931 2220 Size: 2711 4091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2921 4610 276 Size: 53 83 186 936 1387 1689 2468 Fragments arranged by size: 2468 1689 1387 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI