################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AlwNI 1380 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 1489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1473 GGATCC G^GATCC BbvCI 146 CCTCAGC CCTCAGC(-5/-2) BcgI 1611 GCACCCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1218 GGATAC GTATCC(6/5) BglI 919 GCCAATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 3 GTGCCC G[AGT]GC[ACT]^C BpmI 1634 CTGGAG CTGGAG(16/14) BsaBI 1357 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 1408 CTCCTC GAGGAG(10/8) BsgI 616 CTGCAC GTGCAG(16/14) BsmI 1560 GCATTC GAATGC(1/-1) Bsp1286I 3 GTGCCC G[AGT]GC[ACT]^C BsrBI 1405 CCGCTC CCGCTC(-3/-3) BsrDI 1299 CATTGC GCAATG(2/0) BstAPI 1045 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1341 GGTGACC G^GT[ACGT]ACC BstPI 1341 GGTGACC G^GT[ACGT]ACC BstYI 1473 GGATCC [GA]^GATC[CT] Cac8I 638 GCAAGC GC[ACGT]^[ACGT]GC DraIII 1177 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 290 CCTACGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1341 GGTGACC G^GT[ACGT]ACC EcoT14I 116 CCAAGG C^C[AT][AT]GG MaeII 958 ACGT A^CGT MamI 1357 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1473 GGATCC [GA]^GATC[CT] PflFI 1489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SexAI 1453 ACCAGGT A^CC[AT]GGT SspI 753 AATATT AAT^ATT StyI 116 CCAAGG C^C[AT][AT]GG SwaI 698 ATTTAAAT ATTT^AAAT TaqI 1034 TCGA T^CGA Tth111I 1489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1034 TCGA T^CGA XhoII 1473 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 1348 ACATGT A^C[GA][CT]GT 1659 ACATGT BfaI 674 CTAG C^TAG 785 CTAG BspLU11I 1348 ACATGT A^CATGT 1659 ACATGT DpnII 478 GATC ^GATC 1474 GATC DraI 604 TTTAAA TTT^AAA 699 TTTAAA EcoRI 27 GAATTC G^AATTC 1248 GAATTC FauI 916 CCCGC CCCGC(4/6) 1649 GCGGG HapII 925 CCGG C^CGG 1208 CCGG HpaII 925 CCGG C^CGG 1208 CCGG MaeI 674 CTAG C^TAG 785 CTAG MboI 478 GATC ^GATC 1474 GATC MslI 1075 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1225 CATATTTATG MspI 925 CCGG C^CGG 1208 CCGG NdeII 478 GATC ^GATC 1474 GATC NspI 1348 ACATGT [GA]CATG^[CT] 1659 ACATGT PleI 371 GACTC GAGTC(4/5) 358 GAGTC PvuII 149 CAGCTG CAG^CTG 1383 CAGCTG Sau3AI 478 GATC ^GATC 1474 GATC XcmI 257 CCACACCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1139 CCAGTTCTTCTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbsI BbvI BpuAI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboII MnlI MseI MspA1I MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI