(Linear) (Six-base) MAPSORT of: KIBB3035.seq Check: 6413 from: 1 to: 1863 KIBB3035.orf With 182 enzymes: SgfI * April 28, 2014 10:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 732 1157 1431 1863 Size: 732 425 274 432 Fragments arranged by size: 732 432 425 274 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 871 1552 1863 Size: 31 840 681 311 Fragments arranged by size: 840 681 311 31 AflIII A'CryG_T Cuts at: 0 772 1863 Size: 772 1091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1165 1863 Size: 1165 698 AlwNI CAG_nnn'CTG Cuts at: 0 846 849 1863 Size: 846 3 1014 Fragments arranged by size: 1014 846 3 ApaI G_GGCC'C Cuts at: 0 1111 1179 1404 1863 Size: 1111 68 225 459 Fragments arranged by size: 1111 459 225 68 ApaLI G'TGCA_C Cuts at: 0 1418 1863 Size: 1418 445 AvaI C'yCGr_G Cuts at: 0 1104 1170 1397 1709 1863 Size: 1104 66 227 312 154 Fragments arranged by size: 1104 312 227 154 66 BanI G'GyrC_C Cuts at: 0 516 574 1863 Size: 516 58 1289 Fragments arranged by size: 1289 516 58 BanII G_rGCy'C Cuts at: 0 1044 1111 1169 1179 1404 1449 1690 1863 Size: 1044 67 58 10 225 45 241 173 Fragments arranged by size: 1044 241 225 173 67 58 45 10 BbvCI CC'TCA_GC Cuts at: 0 1132 1385 1863 Size: 1132 253 478 Fragments arranged by size: 1132 478 253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1570 1604 1714 1748 1863 Size: 1570 34 110 34 115 Fragments arranged by size: 1570 115 110 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 106 932 1158 1220 1311 1863 Size: 106 826 226 62 91 552 Fragments arranged by size: 826 552 226 106 91 62 BlpI GC'TnA_GC Cuts at: 0 668 1043 1863 Size: 668 375 820 Fragments arranged by size: 820 668 375 Bme1580I G_kGCm'C Cuts at: 0 102 185 521 1076 1111 1179 1302 1404 Size: 102 83 336 555 35 68 123 102 Cuts at: 1404 1422 1863 Size: 18 441 Fragments arranged by size: 555 441 336 123 102 102 83 68 35 18 BmgBI CAC'GTC Cuts at: 0 1502 1508 1863 Size: 1502 6 355 Fragments arranged by size: 1502 355 6 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 285 473 721 906 1122 1183 1863 Size: 285 188 248 185 216 61 680 Fragments arranged by size: 680 285 248 216 188 185 61 Bpu10I CC'TnA_GC Cuts at: 0 761 1132 1385 1863 Size: 761 371 253 478 Fragments arranged by size: 761 478 371 253 BsaI GGTCTCn'nnnn_ Cuts at: 0 902 1863 Size: 902 961 BsaAI yAC'GTr Cuts at: 0 81 773 1863 Size: 81 692 1090 Fragments arranged by size: 1090 692 81 BsaHI Gr'CG_yC Cuts at: 0 1516 1661 1863 Size: 1516 145 202 Fragments arranged by size: 1516 202 145 BsaWI w'CCGG_w Cuts at: 0 866 1863 Size: 866 997 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 417 447 1863 Size: 417 30 1416 Fragments arranged by size: 1416 417 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 427 430 724 1539 1708 1863 Size: 427 3 294 815 169 155 Fragments arranged by size: 815 427 294 169 155 3 BseYI C'CCAG_C Cuts at: 0 197 1065 1254 1301 1306 1403 1449 1652 Size: 197 868 189 47 5 97 46 203 Cuts at: 1652 1664 1863 Size: 12 199 Fragments arranged by size: 868 203 199 197 189 97 47 46 12 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 826 1600 1777 1863 Size: 619 207 774 177 86 Fragments arranged by size: 774 619 207 177 86 BsiEI CG_ry'CG Cuts at: 0 255 1224 1863 Size: 255 969 639 Fragments arranged by size: 969 639 255 BsiHKAI G_wGCw'C Cuts at: 0 863 892 1044 1238 1422 1863 Size: 863 29 152 194 184 441 Fragments arranged by size: 863 441 194 184 152 29 BsmBI CGTCTCn'nnnn_ Cuts at: 0 412 1507 1863 Size: 412 1095 356 Fragments arranged by size: 1095 412 356 Bsp1286I G_dGCh'C Cuts at: 0 102 185 521 863 892 1044 1076 1111 Size: 102 83 336 342 29 152 32 35 Cuts at: 1111 1169 1179 1238 1302 1404 1422 1449 1690 Size: 58 10 59 64 102 18 27 241 Cuts at: 1690 1863 Size: 173 Fragments arranged by size: 342 336 241 173 152 102 102 83 64 59 58 35 32 29 27 18 10 BspEI T'CCGG_A Cuts at: 0 866 1863 Size: 866 997 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 732 1157 1431 1486 1622 1799 1863 Size: 732 425 274 55 136 177 64 Fragments arranged by size: 732 425 274 177 136 64 55 BsrDI GCAATG_nn' Cuts at: 0 1209 1863 Size: 1209 654 BsrGI T'GTAC_A Cuts at: 0 775 794 1863 Size: 775 19 1069 Fragments arranged by size: 1069 775 19 BstAPI GCAn_nnn'nTGC Cuts at: 0 1722 1863 Size: 1722 141 BstXI CCAn_nnnn'nTGG Cuts at: 0 549 1863 Size: 549 1314 Bsu36I CC'TnA_GG Cuts at: 0 103 1863 Size: 103 1760 BtgI C'CryG_G Cuts at: 0 1156 1863 Size: 1156 707 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1809 1863 Size: 1809 54 BtsI GCAGTG_nn' Cuts at: 0 778 1863 Size: 778 1085 DraIII CAC_nnn'GTG Cuts at: 0 359 1863 Size: 359 1504 EaeI y'GGCC_r Cuts at: 0 252 746 1080 1190 1221 1863 Size: 252 494 334 110 31 642 Fragments arranged by size: 642 494 334 252 110 31 EagI C'GGCC_G Cuts at: 0 252 1221 1863 Size: 252 969 642 Fragments arranged by size: 969 642 252 EarI CTCTTCn'nnn_ Cuts at: 0 562 1557 1863 Size: 562 995 306 Fragments arranged by size: 995 562 306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 424 724 1863 Size: 424 300 1139 Fragments arranged by size: 1139 424 300 EcoICRI GAG'CTC Cuts at: 0 1042 1863 Size: 1042 821 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 285 473 721 871 906 1122 1183 Size: 31 254 188 248 150 35 216 61 Cuts at: 1183 1552 1863 Size: 369 311 Fragments arranged by size: 369 311 254 248 216 188 150 61 35 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 703 739 1863 Size: 703 36 1124 Fragments arranged by size: 1124 703 36 EcoO109I rG'GnC_Cy Cuts at: 0 107 614 1175 1863 Size: 107 507 561 688 Fragments arranged by size: 688 561 507 107 EcoRV GAT'ATC Cuts at: 0 1022 1863 Size: 1022 841 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1599 1863 Size: 1599 264 HaeII r_GCGC'y Cuts at: 0 10 948 1566 1863 Size: 10 938 618 297 Fragments arranged by size: 938 618 297 10 HincII GTy'rAC Cuts at: 0 1056 1863 Size: 1056 807 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 269 1490 1863 Size: 269 1221 373 Fragments arranged by size: 1221 373 269 MscI TGG'CCA Cuts at: 0 748 1863 Size: 748 1115 MspA1I CmG'CkG Cuts at: 0 599 846 1158 1220 1781 1842 1848 1863 Size: 599 247 312 62 561 61 6 15 Fragments arranged by size: 599 561 312 247 62 61 15 6 NspI r_CATG'y Cuts at: 0 795 1863 Size: 795 1068 PasI CC'CwG_GG Cuts at: 0 267 1683 1863 Size: 267 1416 180 Fragments arranged by size: 1416 267 180 PflMI CCAn_nnn'nTGG Cuts at: 0 744 1863 Size: 744 1119 PfoI T'CCnGG_A Cuts at: 0 523 1863 Size: 523 1340 PmlI CAC'GTG Cuts at: 0 81 773 1863 Size: 81 692 1090 Fragments arranged by size: 1090 692 81 PspOMI G'GGCC_C Cuts at: 0 1107 1175 1400 1863 Size: 1107 68 225 463 Fragments arranged by size: 1107 463 225 68 PspXI vC'TCGA_Gb Cuts at: 0 1709 1863 Size: 1709 154 PstI C_TGCA'G Cuts at: 0 689 785 977 1483 1853 1863 Size: 689 96 192 506 370 10 Fragments arranged by size: 689 506 370 192 96 10 PvuII CAG'CTG Cuts at: 0 846 1848 1863 Size: 846 1002 15 Fragments arranged by size: 1002 846 15 SacI G_AGCT'C Cuts at: 0 1044 1863 Size: 1044 819 SacII CC_GC'GG Cuts at: 0 1159 1863 Size: 1159 704 SfcI C'TryA_G Cuts at: 0 685 781 973 1479 1761 1849 1863 Size: 685 96 192 506 282 88 14 Fragments arranged by size: 685 506 282 192 96 88 14 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1220 1863 Size: 1220 643 SmaI CCC'GGG Cuts at: 0 1106 1399 1863 Size: 1106 293 464 Fragments arranged by size: 1106 464 293 SmlI C'TyrA_G Cuts at: 0 1709 1863 Size: 1709 154 StuI AGG'CCT Cuts at: 0 1090 1863 Size: 1090 773 StyI C'CwwG_G Cuts at: 0 177 903 1863 Size: 177 726 960 Fragments arranged by size: 960 726 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 641 1444 1666 1863 Size: 641 803 222 197 Fragments arranged by size: 803 641 222 197 TatI w'GTAC_w Cuts at: 0 775 794 1595 1863 Size: 775 19 801 268 Fragments arranged by size: 801 775 268 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 417 449 558 590 757 789 1863 Size: 417 32 109 32 167 32 1074 Fragments arranged by size: 1074 417 167 109 32 32 32 XhoI C'TCGA_G Cuts at: 0 1709 1863 Size: 1709 154 XmaI C'CCGG_G Cuts at: 0 1104 1397 1863 Size: 1104 293 466 Fragments arranged by size: 1104 466 293 XmnI GAAnn'nnTTC Cuts at: 0 1582 1863 Size: 1582 281 Enzymes that do cut: AarI AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbvCI BcgI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII HincII MmeI MscI MspA1I NspI PasI PflMI PfoI PmlI PspOMI PspXI PstI PvuII SacI SacII SfcI SfiI SmaI SmlI StuI StyI TaqII TatI TstI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaBI BsiWI BsmI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DrdI EcoRI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI ZraI