(Circular) (Six-base) MAPSORT of: pFN21AB9878.seq Check: 221 from: 1 to: 6430 pFN21AB9878 6430 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2193 3677 2193 Size: 1484 4946 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2813 4240 278 Size: 53 83 186 936 1277 1427 2468 Fragments arranged by size: 2468 1427 1277 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3252 3773 1937 Size: 1315 521 4594 Fragments arranged by size: 4594 1315 521 Acc65I G'GTAC_C Cuts at: 3035 3751 3035 Size: 716 5714 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 3197 3264 3519 4493 5877 6145 1632 Size: 749 816 67 255 974 1384 268 1917 Fragments arranged by size: 1917 1384 974 816 749 268 255 67 AfeI AGC'GCT Cuts at: 3625 3625 Size: 6430 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 2108 1166 Size: 942 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 4173 5745 4173 Size: 1572 4858 ApaI G_GGCC'C Cuts at: 2221 2221 Size: 6430 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 3739 3965 4117 6226 1493 Size: 2246 226 152 2109 1697 Fragments arranged by size: 2246 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2381 2684 2703 3164 3755 1870 Size: 73 231 207 303 19 461 591 4545 Fragments arranged by size: 4545 591 461 303 231 207 73 19 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 2170 3035 3751 5206 Size: 325 217 627 309 74 865 716 1455 Cuts at: 5206 618 Size: 1842 Fragments arranged by size: 1842 1455 865 716 627 325 309 217 74 BanII G_rGCy'C Cuts at: 1391 2133 2221 2693 3549 3749 1391 Size: 742 88 472 856 200 4072 Fragments arranged by size: 4072 856 742 472 200 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2369 3109 928 Size: 971 470 740 4249 Fragments arranged by size: 4249 971 740 470 BbvCI CC'TCA_GC Cuts at: 2018 2047 2073 2018 Size: 29 26 6375 Fragments arranged by size: 6375 29 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2698 2732 4621 4655 1903 Size: 34 761 34 1889 34 3678 Fragments arranged by size: 3678 1889 761 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5041 136 Size: 107 122 71 631 605 3369 1525 Fragments arranged by size: 3369 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 3847 3847 Size: 6430 Bme1580I G_kGCm'C Cuts at: 1790 2101 2175 2221 2532 4491 5647 1790 Size: 311 74 46 311 1959 1156 2573 Fragments arranged by size: 2573 1959 1156 311 311 74 46 BmgBI CAC'GTC Cuts at: 1318 2847 1318 Size: 1529 4901 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3353 3490 4161 Size: 416 53 83 399 24 1936 137 671 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 1936 1753 958 671 416 399 137 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2783 2854 3226 5090 1239 Size: 971 573 71 372 1864 2579 Fragments arranged by size: 2579 1864 971 573 372 71 Bpu10I CC'TnA_GC Cuts at: 2018 2047 2073 2018 Size: 29 26 6375 Fragments arranged by size: 6375 29 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2211 4555 5420 5718 2211 Size: 2344 865 298 2923 Fragments arranged by size: 2923 2344 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5093 882 Size: 617 3594 2219 Fragments arranged by size: 3594 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3508 4221 493 Size: 711 2304 713 2702 Fragments arranged by size: 2702 2304 713 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2475 2810 3182 4237 Size: 53 83 186 936 942 335 372 1055 Cuts at: 4237 4619 5254 275 Size: 382 635 1451 Fragments arranged by size: 1451 1055 942 936 635 382 372 335 186 83 53 BsaWI w'CCGG_w Cuts at: 4863 5261 5535 5682 6093 4863 Size: 398 274 147 411 5200 Fragments arranged by size: 5200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2528 2558 2891 2921 1178 Size: 30 165 30 1125 30 333 30 4687 Fragments arranged by size: 4687 1125 333 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2061 2564 2591 2636 2639 3520 3557 1186 Size: 875 503 27 45 3 881 37 4059 Fragments arranged by size: 4059 881 875 503 45 37 27 3 BseYI C'CCAG_C Cuts at: 2080 2392 2465 2569 3196 5633 6129 2080 Size: 312 73 104 627 2437 496 2381 Fragments arranged by size: 2437 2381 627 496 312 104 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3655 1163 Size: 573 1919 3938 Fragments arranged by size: 3938 1919 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2257 2289 2455 2519 3880 4641 Size: 275 186 256 32 166 64 1361 761 Cuts at: 4641 5669 1540 Size: 1028 2301 Fragments arranged by size: 2301 1361 1028 761 275 256 186 166 64 32 BsiHKAI G_wGCw'C Cuts at: 2693 3549 4491 4576 5647 2693 Size: 856 942 85 1071 3476 Fragments arranged by size: 3476 1071 942 856 85 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 BsmBI CGTCTCn'nnnn_ Cuts at: 2345 2378 2759 2894 2345 Size: 33 381 135 5881 Fragments arranged by size: 5881 381 135 33 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2133 2175 2221 2532 2693 3549 Size: 399 311 32 42 46 311 161 856 Cuts at: 3549 3749 4491 4576 5647 1391 Size: 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 856 742 399 311 311 200 161 85 46 42 32 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 2985 4319 6049 2985 Size: 1334 1730 3366 Fragments arranged by size: 3366 1730 1334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 3677 3785 844 Size: 1349 1484 108 3489 Fragments arranged by size: 3489 1484 1349 108 BsrBI CCG'CTC Cuts at: 1394 1823 2431 2629 4317 1394 Size: 429 608 198 1688 3507 Fragments arranged by size: 3507 1688 608 429 198 BsrDI GCAATG_nn' Cuts at: 65 1248 3067 3422 4925 5099 65 Size: 1183 1819 355 1503 174 1396 Fragments arranged by size: 1819 1503 1396 1183 355 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2284 2460 2514 2518 2698 2834 3404 Size: 110 474 176 54 4 180 136 570 Cuts at: 3404 5074 5261 1700 Size: 1670 187 2869 Fragments arranged by size: 2869 1670 570 474 187 180 176 136 110 54 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 2826 2928 5234 2826 Size: 102 2306 4022 Fragments arranged by size: 4022 2306 102 BstEII G'GTnAC_C Cuts at: 1184 3322 1184 Size: 2138 4292 BstXI CCAn_nnnn'nTGG Cuts at: 3082 3082 Size: 6430 BstYI r'GATC_y Cuts at: 1972 2553 3159 3386 3760 4127 4512 4529 5981 Size: 581 606 227 374 367 385 17 1452 Cuts at: 5981 6067 6079 6425 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 606 581 385 374 367 346 227 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3092 3092 Size: 6430 BtgI C'CryG_G Cuts at: 513 1065 2004 2116 2166 2289 2583 513 Size: 552 939 112 50 123 294 4360 Fragments arranged by size: 4360 939 552 294 123 112 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2695 3385 3403 487 Size: 683 56 1469 690 18 3514 Fragments arranged by size: 3514 1469 690 683 56 18 BtsI GCAGTG_nn' Cuts at: 2279 3324 3660 3946 4740 4760 2279 Size: 1045 336 286 794 20 3949 Fragments arranged by size: 3949 1045 794 336 286 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DraIII CAC_nnn'GTG Cuts at: 3361 3361 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2286 2516 Size: 54 1425 50 275 18 372 84 230 Cuts at: 2516 3006 3045 3084 3877 4766 6395 8 Size: 490 39 39 793 889 1629 43 Fragments arranged by size: 1629 1425 889 793 490 372 275 230 84 54 50 43 39 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2286 2516 3877 1537 Size: 275 474 230 1361 4090 Fragments arranged by size: 4090 1361 474 275 230 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 3480 3538 3597 4360 1353 Size: 1325 802 58 59 763 3423 Fragments arranged by size: 3423 1325 802 763 59 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2501 3092 5017 5391 5537 387 Size: 1064 138 912 591 1925 374 146 1280 Fragments arranged by size: 1925 1280 1064 912 591 374 146 138 EcoICRI GAG'CTC Cuts at: 2691 3547 2691 Size: 856 5574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2381 2783 2854 3197 3226 3264 Size: 393 578 171 402 71 343 29 38 Cuts at: 3264 3519 4493 5090 5877 6145 1239 Size: 255 974 597 787 268 1524 Fragments arranged by size: 1524 974 787 597 578 402 393 343 268 255 171 71 38 29 EcoNI CCTnn'n_nnAGG Cuts at: 2618 2618 Size: 6430 EcoO109I rG'GnC_Cy Cuts at: 2217 2218 2853 2217 Size: 1 635 5794 Fragments arranged by size: 5794 635 1 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 FseI GG_CCGG'CC Cuts at: 2702 2702 Size: 6430 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2017 2080 2659 3627 5577 1678 Size: 339 63 579 968 1950 2531 Fragments arranged by size: 2531 1950 968 579 339 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 3192 3224 5071 Size: 32 135 32 956 32 632 32 1847 Cuts at: 5071 5103 5145 5177 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1847 956 632 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2524 3703 3774 4020 669 Size: 988 281 586 1179 71 246 3079 Fragments arranged by size: 3079 1179 988 586 281 246 71 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3039 3755 3039 Size: 716 5714 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3471 3564 5544 5728 1305 Size: 2166 93 1980 184 2007 Fragments arranged by size: 2166 2007 1980 184 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 3008 3086 10 Size: 54 1425 343 1176 78 3354 Fragments arranged by size: 3354 1425 1176 343 78 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2165 3506 4388 4747 4906 518 Size: 648 942 57 1341 882 359 159 2042 Fragments arranged by size: 2042 1341 942 882 648 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2091 2269 2291 2359 2569 3846 4523 5671 Size: 485 178 22 68 210 1277 677 1148 Cuts at: 5671 5916 1606 Size: 245 2120 Fragments arranged by size: 2120 1277 1148 677 485 245 210 178 68 22 NaeI GCC'GGC Cuts at: 2286 2516 2700 2836 2286 Size: 230 184 136 5880 Fragments arranged by size: 5880 230 184 136 NcoI C'CATG_G Cuts at: 513 1065 2004 2583 513 Size: 552 939 579 4360 Fragments arranged by size: 4360 939 579 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 2284 2514 2698 2834 2284 Size: 230 184 136 5880 Fragments arranged by size: 5880 230 184 136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NruI TCG'CGA Cuts at: 2977 2977 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3505 3788 5333 1132 Size: 97 2276 283 1545 2229 Fragments arranged by size: 2276 2229 1545 283 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6430 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2107 2188 1108 Size: 999 81 5350 Fragments arranged by size: 5350 999 81 PfoI T'CCnGG_A Cuts at: 3297 3297 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PmlI CAC'GTG Cuts at: 3508 3508 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2853 2853 Size: 6430 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2217 2217 Size: 6430 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2105 2137 2105 Size: 32 6398 PstI C_TGCA'G Cuts at: 830 3159 3782 830 Size: 2329 623 3478 Fragments arranged by size: 3478 2329 623 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2359 2569 2359 Size: 210 6220 SacI G_AGCT'C Cuts at: 2693 3549 2693 Size: 856 5574 SacII CC_GC'GG Cuts at: 2292 2292 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SanDI GG'GwC_CC Cuts at: 2853 2853 Size: 6430 SapI GCTCTTCn'nnn_ Cuts at: 2678 3480 2678 Size: 802 5628 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 2444 4678 6390 2444 Size: 2234 1712 2484 Fragments arranged by size: 2484 2234 1712 SfcI C'TryA_G Cuts at: 826 1045 3155 3778 4913 5594 5785 826 Size: 219 2110 623 1135 681 191 1471 Fragments arranged by size: 2110 1471 1135 681 623 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 3404 1700 Size: 1704 4726 SmaI CCC'GGG Cuts at: 1872 2176 2705 3757 1872 Size: 304 529 1052 4545 Fragments arranged by size: 4545 1052 529 304 SmlI C'TyrA_G Cuts at: 820 1017 1943 2226 4534 5435 5697 820 Size: 197 926 283 2308 901 262 1553 Fragments arranged by size: 2308 1553 926 901 283 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6430 StyI C'CwwG_G Cuts at: 513 1065 2004 2583 3147 3869 513 Size: 552 939 579 564 722 3074 Fragments arranged by size: 3074 939 722 579 564 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3372 4457 4474 4627 4812 5257 325 Size: 1255 1792 1085 17 153 185 445 1498 Fragments arranged by size: 1792 1498 1255 1085 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 3498 4676 Size: 274 80 33 51 1443 465 1056 1178 Cuts at: 4676 6388 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1178 1056 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 6390 6422 2891 Size: 32 3467 32 2899 Fragments arranged by size: 3467 2899 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3193 3334 3193 Size: 141 6289 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 2174 2703 3755 1870 Size: 304 529 1052 4545 Fragments arranged by size: 4545 1052 529 304 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2811 4238 276 Size: 53 83 186 936 1277 1427 2468 Fragments arranged by size: 2468 1427 1277 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FspAI KasI NarI NsiI PacI PspXI RsrII SexAI SfiI SfoI SrfI SwaI