################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV FseI FspI HapII HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 820 GACGTC GACGT^C AciI 984 GCGG CCGC(-3/-1) AcsI 1155 GAATTT [GA]^AATT[CT] AhdI 214 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1023 CCTGAGG CC^T[ACGT]AGG ApoI 1155 GAATTT [GA]^AATT[CT] AspEI 214 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 921 GTGCTC G[AT]GC[AT]^C AspI 382 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 156 TGGCCA TGG^CCA BamHI 411 GGATCC G^GATCC BclI 201 TGATCA T^GATCA BfaI 241 CTAG C^TAG BlpI 192 GCTCAGC GC^T[ACGT]AGC BmyI 921 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 192 GCTCAGC GC^T[ACGT]AGC BsaJI 260 CCATGG C^C[ACGT][ACGT]GG BseRI 1026 GAGGAG GAGGAG(10/8) BsgI 788 GTGCAG GTGCAG(16/14) BsiHKAI 921 GTGCTC G[AT]GC[AT]^C BsiYI 1194 CCTTTTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1194 CCTTTTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 578 GGGAC GGGAC(10/14) Bsp1286I 921 GTGCTC G[AGT]GC[ACT]^C BstXI 344 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 411 GGATCC [GA]^GATC[CT] Bsu36I 1023 CCTGAGG CC^T[ACGT]AGG CelII 192 GCTCAGC GC^T[ACGT]AGC DsaI 260 CCATGG C^C[GA][CT]GG EaeI 156 TGGCCA [CT]^GGCC[GA] Eam1105I 214 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1023 CCTGAGG CC^T[ACGT]AGG EcoNI 1194 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 260 CCATGG C^C[AT][AT]GG EcoT22I 252 ATGCAT ATGCA^T FauI 984 GCGGG CCCGC(4/6) FbaI 201 TGATCA T^GATCA HindIII 989 AAGCTT A^AGCTT MaeI 241 CTAG C^TAG MflI 411 GGATCC [GA]^GATC[CT] MluNI 156 TGGCCA TGG^CCA MscI 156 TGGCCA TGG^CCA MspA1I 518 CAGCTG C[AC]G^C[GT]G NcoI 260 CCATGG C^CATGG NdeI 266 CATATG CA^TATG NlaIV 411 GGATCC GG[ACGT]^[ACGT]CC NsiI 252 ATGCAT ATGCA^T PflFI 382 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 474 CTGCAG CTGCA^G PvuII 518 CAGCTG CAG^CTG SfcI 474 CTGCAG C^T[GA][CT]AG SspI 316 AATATT AAT^ATT StyI 260 CCATGG C^C[AT][AT]GG TaqI 165 TCGA T^CGA Tth111I 382 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 165 TCGA T^CGA XcmI 837 CCAGCAGACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 411 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 820 GACGTC G[GA]^CG[CT]C 997 GACGCC AlwI 412 GATCC GGATC(4/5) 411 GGATC BpmI 874 CTCCAG CTGGAG(16/14) 1136 CTGGAG BsaHI 820 GACGTC G[GA]^CG[CT]C 997 GACGCC BsaI 495 GGTCTC GGTCTC(1/5) 1017 GGTCTC BsrDI 123 CATTGC GCAATG(2/0) 559 GCAATG CfoI 228 GCGC GCG^C 595 GCGC HaeII 227 AGCGCC [GA]GCGC^[CT] 594 GGCGCT HgaI 997 GACGC GACGC(5/10) 803 GCGTC HhaI 228 GCGC GCG^C 595 GCGC Hin1I 820 GACGTC G[GA]^CG[CT]C 997 GACGCC HinP1I 228 GCGC G^CGC 595 GCGC MaeIII 222 GTCAC ^GT[ACGT]AC 1048 GTGAC MseI 184 TTAA T^TAA 1150 TTAA NspI 188 ACATGC [GA]CATG^[CT] 858 ACATGC PleI 151 GAGTC GAGTC(4/5) 931 GAGTC TfiI 485 GATTC G^A[AT]TC 1065 GATTC Tru9I 184 TTAA T^TAA 1150 TTAA Tsp45I 222 GTCAC ^GT[GC]AC 1048 GTGAC Tsp509I 540 AATT ^AATT 1156 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI BbsI BbvI BpuAI BsmAI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI TseI TspRI