(Linear) (Six-base) MAPSORT of: KIEE1254.seq Check: 7410 from: 1 to: 1641 KIEE1254.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AccI GT'mk_AC Cuts at: 0 4 349 1641 Size: 4 345 1292 Fragments arranged by size: 1292 345 4 AclI AA'CG_TT Cuts at: 0 1074 1641 Size: 1074 567 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 719 985 1641 Size: 109 610 266 656 Fragments arranged by size: 656 610 266 109 AhdI GACnn_n'nnGTC Cuts at: 0 734 1641 Size: 734 907 AleI CACnn'nnGTG Cuts at: 0 575 721 1641 Size: 575 146 920 Fragments arranged by size: 920 575 146 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 538 570 1641 Size: 538 32 1071 Fragments arranged by size: 1071 538 32 ApoI r'AATT_y Cuts at: 0 519 1491 1641 Size: 519 972 150 Fragments arranged by size: 972 519 150 AseI AT'TA_AT Cuts at: 0 440 1641 Size: 440 1201 BamHI G'GATC_C Cuts at: 0 851 1641 Size: 851 790 BanII G_rGCy'C Cuts at: 0 873 1641 Size: 873 768 BbsI GAAGACnn'nnnn_ Cuts at: 0 119 287 401 733 1532 1641 Size: 119 168 114 332 799 109 Fragments arranged by size: 799 332 168 119 114 109 BbvCI CC'TCA_GC Cuts at: 0 985 1641 Size: 985 656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 745 779 1641 Size: 745 34 862 Fragments arranged by size: 862 745 34 BciVI GTATCCnnnnn_n' Cuts at: 0 988 1096 1641 Size: 988 108 545 Fragments arranged by size: 988 545 108 BglI GCCn_nnn'nGGC Cuts at: 0 1411 1641 Size: 1411 230 Bme1580I G_kGCm'C Cuts at: 0 1407 1641 Size: 1407 234 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1256 1577 1641 Size: 1256 321 64 Fragments arranged by size: 1256 321 64 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 859 1641 Size: 859 782 Bpu10I CC'TnA_GC Cuts at: 0 148 985 1641 Size: 148 837 656 Fragments arranged by size: 837 656 148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 1641 Size: 691 950 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 355 385 1248 1278 1641 Size: 355 30 863 30 363 Fragments arranged by size: 863 363 355 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 52 97 370 373 376 379 382 400 Size: 52 45 273 3 3 3 3 18 Cuts at: 400 1245 1253 1256 1259 1268 1271 1274 1277 Size: 845 8 3 3 9 3 3 3 Cuts at: 1277 1313 1316 1319 1328 1641 Size: 36 3 3 9 313 Fragments arranged by size: 845 313 273 52 45 36 18 9 9 8 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 255 558 990 1641 Size: 255 303 432 651 Fragments arranged by size: 651 432 303 255 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 785 1641 Size: 785 856 BsiHKAI G_wGCw'C Cuts at: 0 806 873 893 1641 Size: 806 67 20 748 Fragments arranged by size: 806 748 67 20 Bsp1286I G_dGCh'C Cuts at: 0 806 873 893 1407 1641 Size: 806 67 20 514 234 Fragments arranged by size: 806 514 234 67 20 BspHI T'CATG_A Cuts at: 0 863 1563 1641 Size: 863 700 78 Fragments arranged by size: 863 700 78 BsrDI GCAATG_nn' Cuts at: 0 543 630 776 1373 1641 Size: 543 87 146 597 268 Fragments arranged by size: 597 543 268 146 87 BstYI r'GATC_y Cuts at: 0 851 1641 Size: 851 790 BstZ17I GTA'TAC Cuts at: 0 350 1641 Size: 350 1291 BtgI C'CryG_G Cuts at: 0 140 770 1641 Size: 140 630 871 Fragments arranged by size: 871 630 140 DraI TTT'AAA Cuts at: 0 498 1641 Size: 498 1143 EaeI y'GGCC_r Cuts at: 0 740 773 857 1253 1641 Size: 740 33 84 396 388 Fragments arranged by size: 740 396 388 84 33 EarI CTCTTCn'nnn_ Cuts at: 0 15 296 377 401 1164 1641 Size: 15 281 81 24 763 477 Fragments arranged by size: 763 477 281 81 24 15 EcoICRI GAG'CTC Cuts at: 0 871 1641 Size: 871 770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 719 859 985 1641 Size: 109 610 140 126 656 Fragments arranged by size: 656 610 140 126 109 EcoO109I rG'GnC_Cy Cuts at: 0 1114 1641 Size: 1114 527 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 322 1641 Size: 322 1319 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 355 387 720 752 1221 1253 1641 Size: 355 32 333 32 469 32 388 Fragments arranged by size: 469 388 355 333 32 32 32 MfeI C'AATT_G Cuts at: 0 419 1641 Size: 419 1222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 89 854 1641 Size: 89 765 787 Fragments arranged by size: 787 765 89 MscI TGG'CCA Cuts at: 0 742 775 859 1255 1641 Size: 742 33 84 396 386 Fragments arranged by size: 742 396 386 84 33 MslI CAynn'nnrTG Cuts at: 0 575 721 1641 Size: 575 146 920 Fragments arranged by size: 920 575 146 MspA1I CmG'CkG Cuts at: 0 562 1641 Size: 562 1079 NcoI C'CATG_G Cuts at: 0 770 1641 Size: 770 871 NspI r_CATG'y Cuts at: 0 754 1641 Size: 754 887 PflMI CCAn_nnn'nTGG Cuts at: 0 1136 1641 Size: 1136 505 PpuMI rG'GwC_Cy Cuts at: 0 1114 1641 Size: 1114 527 PshAI GACnn'nnGTC Cuts at: 0 1132 1641 Size: 1132 509 PvuII CAG'CTG Cuts at: 0 562 1641 Size: 562 1079 SacI G_AGCT'C Cuts at: 0 873 1641 Size: 873 768 SapI GCTCTTCn'nnn_ Cuts at: 0 296 1164 1641 Size: 296 868 477 Fragments arranged by size: 868 477 296 SfcI C'TryA_G Cuts at: 0 587 1641 Size: 587 1054 SmlI C'TyrA_G Cuts at: 0 706 1641 Size: 706 935 SphI G_CATG'C Cuts at: 0 754 1641 Size: 754 887 StyI C'CwwG_G Cuts at: 0 770 1641 Size: 770 871 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1248 1280 1641 Size: 1248 32 361 Fragments arranged by size: 1248 361 32 XmnI GAAnn'nnTTC Cuts at: 0 730 1583 1641 Size: 730 853 58 Fragments arranged by size: 853 730 58 Enzymes that do cut: AccI AclI AcuI AhdI AleI AloI ApoI AseI BamHI BanII BbsI BbvCI BcgI BciVI BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI BstYI BstZ17I BtgI DraI EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MfeI MmeI MscI MslI MspA1I NcoI NspI PflMI PpuMI PshAI PvuII SacI SapI SfcI SmlI SphI StyI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AflIII AgeI AlfI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanI BbeI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI ZraI