(Circular) (Six-base) MAPSORT of: pFN21AE9255.seq Check: 9626 from: 1 to: 8296 pFN21AE9255 8296 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2311 Size: 8296 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6106 278 Size: 53 83 186 936 4570 2468 Fragments arranged by size: 4570 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2887 3666 5639 1937 Size: 950 779 1973 4594 Fragments arranged by size: 4594 1973 950 779 Acc65I G'GTAC_C Cuts at: 5617 5617 Size: 8296 AclI AA'CG_TT Cuts at: 1563 3810 5466 6423 6796 1563 Size: 2247 1656 957 373 3063 Fragments arranged by size: 3063 2247 1656 957 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2751 2777 3135 3330 3836 4194 4797 4895 Size: 1119 26 358 195 506 358 603 98 Cuts at: 4895 5007 6359 7743 8011 1632 Size: 112 1352 1384 268 1917 Fragments arranged by size: 1917 1384 1352 1119 603 506 358 358 268 195 112 98 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8099 AflIII A'CryG_T Cuts at: 1203 5584 7195 8172 1203 Size: 4381 1611 977 1327 Fragments arranged by size: 4381 1611 1327 977 AgeI A'CCGG_T Cuts at: 7127 7127 Size: 8296 AhdI GACnn_n'nnGTC Cuts at: 5128 7025 5128 Size: 1897 6399 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8296 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3162 1184 Size: 1978 6318 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2277 2309 2277 Size: 32 8264 AlwNI CAG_nnn'CTG Cuts at: 4358 4985 5395 6039 7611 4358 Size: 627 410 644 1572 5043 Fragments arranged by size: 5043 1572 644 627 410 ApaLI G'TGCA_C Cuts at: 6353 7509 6353 Size: 1156 7140 ApoI r'AATT_y Cuts at: 1493 3744 5046 5605 5831 5983 8092 1493 Size: 2251 1302 559 226 152 2109 1697 Fragments arranged by size: 2251 2109 1697 1302 559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4827 5294 6850 160 Size: 872 3795 467 1556 1606 Fragments arranged by size: 3795 1606 1556 872 467 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8296 AvaI C'yCGr_G Cuts at: 1870 1943 5621 1870 Size: 73 3678 4545 Fragments arranged by size: 4545 3678 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2727 2760 2727 Size: 33 8263 BamHI G'GATC_C Cuts at: 3538 5626 5993 3538 Size: 2088 367 5841 Fragments arranged by size: 5841 2088 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5617 7072 618 Size: 325 217 627 3830 1455 1842 Fragments arranged by size: 3830 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4096 4491 5615 1391 Size: 2705 395 1124 4072 Fragments arranged by size: 4072 2705 1124 395 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5070 928 Size: 971 3171 4154 Fragments arranged by size: 4154 3171 971 BbvCI CC'TCA_GC Cuts at: 2754 4581 2754 Size: 1827 6469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3443 3477 6487 6521 1903 Size: 34 50 34 1422 34 3010 34 3678 Fragments arranged by size: 3678 3010 1422 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4807 6188 7398 4807 Size: 1381 1210 5705 Fragments arranged by size: 5705 1381 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8296 BfrBI ATG'CAT Cuts at: 2008 4424 2008 Size: 2416 5880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3257 6907 136 Size: 107 122 71 631 605 1585 3650 1525 Fragments arranged by size: 3650 1585 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8291 8291 Size: 8296 BlpI GC'TnA_GC Cuts at: 2574 2749 2922 5713 2574 Size: 175 173 2791 5157 Fragments arranged by size: 5157 2791 175 173 Bme1580I G_kGCm'C Cuts at: 1790 6357 7513 1790 Size: 4567 1156 2573 Fragments arranged by size: 4567 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8296 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6027 6985 442 Size: 416 53 83 399 24 4610 958 1753 Fragments arranged by size: 4610 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8296 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3801 3665 Size: 136 8160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2463 2901 3660 4187 5228 6956 1239 Size: 1224 438 759 527 1041 1728 2579 Fragments arranged by size: 2579 1728 1224 1041 759 527 438 Bpu10I CC'TnA_GC Cuts at: 2754 4581 2754 Size: 1827 6469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2945 4475 4602 6421 7286 7584 2945 Size: 1530 127 1819 865 298 3657 Fragments arranged by size: 3657 1819 1530 865 298 127 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6959 882 Size: 617 5460 2219 Fragments arranged by size: 5460 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6087 493 Size: 711 4883 2702 Fragments arranged by size: 4883 2702 711 BsaBI GATnn'nnATC Cuts at: 8290 8290 Size: 8296 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6103 6485 7120 275 Size: 53 83 186 936 4570 382 635 1451 Fragments arranged by size: 4570 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6729 7127 7401 7548 7959 6729 Size: 398 274 147 411 7066 Fragments arranged by size: 7066 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3954 3984 5571 5601 1178 Size: 30 165 30 2551 30 1587 30 3873 Fragments arranged by size: 3873 2551 1587 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4286 1186 Size: 3100 5196 BseYI C'CCAG_C Cuts at: 2149 7499 7995 2149 Size: 5350 496 2450 Fragments arranged by size: 5350 2450 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2529 3114 1163 Size: 573 793 585 6345 Fragments arranged by size: 6345 793 585 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5746 6507 7535 1540 Size: 275 186 3745 761 1028 2301 Fragments arranged by size: 3745 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4006 4491 5584 6357 6442 7513 4006 Size: 485 1093 773 85 1071 4789 Fragments arranged by size: 4789 1093 1071 773 485 85 BsmI GAATG_Cn' Cuts at: 5807 5900 5807 Size: 93 8203 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4006 4096 4491 5584 5615 6357 6442 Size: 399 2216 90 395 1093 31 742 85 Cuts at: 6442 7513 1391 Size: 1071 2174 Fragments arranged by size: 2216 2174 1093 1071 742 399 395 90 85 31 BspEI T'CCGG_A Cuts at: 7959 7959 Size: 8296 BspHI T'CATG_A Cuts at: 2296 2839 6185 7915 2296 Size: 543 3346 1730 2677 Fragments arranged by size: 3346 2677 1730 543 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2311 4103 5329 5651 844 Size: 1467 1792 1226 322 3489 Fragments arranged by size: 3489 1792 1467 1226 322 BsrBI CCG'CTC Cuts at: 1394 1823 6183 1394 Size: 429 4360 3507 Fragments arranged by size: 4360 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3867 6791 6965 65 Size: 1183 2619 2924 174 1396 Fragments arranged by size: 2924 2619 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6940 7127 1700 Size: 110 5130 187 2869 Fragments arranged by size: 5130 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8296 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8296 BssSI C'ACGA_G Cuts at: 3462 6356 7368 3462 Size: 2894 1012 4390 Fragments arranged by size: 4390 2894 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3197 794 Size: 2403 5893 BstBI TT'CG_AA Cuts at: 7100 7100 Size: 8296 BstEII G'GTnAC_C Cuts at: 1184 4627 1184 Size: 3443 4853 BstXI CCAn_nnnn'nTGG Cuts at: 2128 3919 5384 5488 2128 Size: 1791 1465 104 4936 Fragments arranged by size: 4936 1791 1465 104 BstYI r'GATC_y Cuts at: 1972 2389 3538 5362 5626 5993 6378 6395 7847 Size: 417 1149 1824 264 367 385 17 1452 Cuts at: 7847 7933 7945 8291 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1824 1452 1149 417 385 367 346 264 86 17 12 BstZ17I GTA'TAC Cuts at: 2888 2888 Size: 8296 Bsu36I CC'TnA_GG Cuts at: 5224 5224 Size: 8296 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7744 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7557 Fragments arranged by size: 7557 683 56 BtsI GCAGTG_nn' Cuts at: 4125 4163 5812 6606 6626 4125 Size: 38 1649 794 20 5795 Fragments arranged by size: 5795 1649 794 38 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8296 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2304 2339 529 Size: 35 1740 35 6486 Fragments arranged by size: 6486 1740 35 35 DraI TTT'AAA Cuts at: 5600 5600 Size: 8296 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7303 809 Size: 507 5987 1802 Fragments arranged by size: 5987 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4849 5743 6632 Size: 54 1425 50 275 18 3019 894 889 Cuts at: 6632 8261 8 Size: 1629 43 Fragments arranged by size: 3019 1629 1425 894 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5743 1537 Size: 275 3931 4090 Fragments arranged by size: 4090 3931 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2399 2601 4095 4170 4479 4617 4983 Size: 734 312 202 1494 75 309 138 366 Cuts at: 4983 5012 5318 6226 1353 Size: 29 306 908 3423 Fragments arranged by size: 3423 1494 908 734 366 312 309 306 202 138 75 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6883 7257 7403 387 Size: 1064 138 5294 374 146 1280 Fragments arranged by size: 5294 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4489 4489 Size: 8296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 2751 2777 2901 3135 3330 3660 Size: 393 831 288 26 124 234 195 330 Cuts at: 3660 3836 4187 4194 4797 4895 5007 5228 6359 Size: 176 351 7 603 98 112 221 1131 Cuts at: 6359 6956 7743 8011 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1131 831 787 603 597 393 351 330 288 268 234 221 195 176 124 112 98 26 7 EcoNI CCTnn'n_nnAGG Cuts at: 2168 5342 2168 Size: 3174 5122 EcoRI G'AATT_C Cuts at: 5605 5983 5605 Size: 378 7918 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2927 3212 3401 5239 2927 Size: 285 189 1838 5984 Fragments arranged by size: 5984 1838 285 189 FspI TGC'GCA Cuts at: 6802 6802 Size: 8296 HaeII r_GCGC'y Cuts at: 1678 7443 1678 Size: 5765 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 2630 2662 4490 Size: 32 135 32 902 32 124 32 1828 Cuts at: 4490 4522 5114 5146 6937 6969 7011 7043 1373 Size: 32 592 32 1791 32 42 32 2626 Fragments arranged by size: 2626 1828 1791 902 592 135 124 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2419 5640 5886 669 Size: 988 281 481 3221 246 3079 Fragments arranged by size: 3221 3079 988 481 281 246 HindIII A'AGCT_T Cuts at: 748 2789 748 Size: 2041 6255 HpaI GTT'AAC Cuts at: 2419 5886 2419 Size: 3467 4829 KpnI G_GTAC'C Cuts at: 5621 5621 Size: 8296 MfeI C'AATT_G Cuts at: 5895 5895 Size: 8296 MluI A'CGCG_T Cuts at: 8172 8172 Size: 8296 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 2184 2642 7410 7594 1305 Size: 840 39 458 4768 184 2007 Fragments arranged by size: 4768 2007 840 458 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 4851 10 Size: 54 1425 343 3019 3455 Fragments arranged by size: 3455 3019 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3144 5429 6254 6613 6772 518 Size: 648 1978 2285 825 359 159 2042 Fragments arranged by size: 2285 2042 1978 825 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2510 2753 3128 3158 3457 4580 5712 6389 Size: 904 243 375 30 299 1123 1132 677 Cuts at: 6389 7537 7782 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1132 1123 904 677 375 299 245 243 30 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7744 NdeI CA'TA_TG Cuts at: 387 3490 387 Size: 3103 5193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8296 NotI GC'GGCC_GC Cuts at: 5743 5743 Size: 8296 NsiI A_TGCA'T Cuts at: 2010 4426 2010 Size: 2416 5880 NspI r_CATG'y Cuts at: 1132 1229 2982 3086 3281 5588 5654 7199 1132 Size: 97 1753 104 195 2307 66 1545 2229 Fragments arranged by size: 2307 2229 1753 1545 195 104 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8296 PciI A'CATG_T Cuts at: 5584 7195 5584 Size: 1611 6685 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8296 PmeI GTTT'AAAC Cuts at: 5600 5600 Size: 8296 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3940 3972 6365 6397 7878 7910 3940 Size: 32 2393 32 1481 32 4326 Fragments arranged by size: 4326 2393 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2060 1613 Size: 447 7849 PsiI TTA'TAA Cuts at: 2786 5866 2786 Size: 3080 5216 PstI C_TGCA'G Cuts at: 830 5648 830 Size: 4818 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8296 PvuII CAG'CTG Cuts at: 2510 2753 3128 3457 4580 2510 Size: 243 375 329 1123 6226 Fragments arranged by size: 6226 1123 375 329 243 SacI G_AGCT'C Cuts at: 4491 4491 Size: 8296 SalI G'TCGA_C Cuts at: 1936 5638 1936 Size: 3702 4594 SapI GCTCTTCn'nnn_ Cuts at: 4479 4983 4479 Size: 504 7792 SbfI CC_TGCA'GG Cuts at: 5648 5648 Size: 8296 ScaI AGT'ACT Cuts at: 6544 8256 6544 Size: 1712 6584 SfcI C'TryA_G Cuts at: 826 1045 2556 3334 5058 5502 5644 6779 7460 Size: 219 1511 778 1724 444 142 1135 681 Cuts at: 7460 7651 826 Size: 191 1471 Fragments arranged by size: 1724 1511 1471 1135 778 681 444 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8296 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8296 SmaI CCC'GGG Cuts at: 1872 5623 1872 Size: 3751 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2960 4490 4617 6400 7301 7563 Size: 197 926 1017 1530 127 1783 901 262 Cuts at: 7563 820 Size: 1553 Fragments arranged by size: 1783 1553 1530 1017 926 901 262 197 127 SnaBI TAC'GTA Cuts at: 493 6087 493 Size: 5594 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8296 SphI G_CATG'C Cuts at: 1132 3086 3281 5654 1132 Size: 1954 195 2373 3774 Fragments arranged by size: 3774 2373 1954 195 SspI AAT'ATT Cuts at: 5 52 2348 5098 6220 5 Size: 47 2296 2750 1122 2081 Fragments arranged by size: 2750 2296 2081 1122 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8296 StyI C'CwwG_G Cuts at: 513 1065 3828 5358 5559 5735 513 Size: 552 2763 1530 201 176 3074 Fragments arranged by size: 3074 2763 1530 552 201 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 5587 6323 6340 6493 6678 7123 Size: 1255 492 3515 736 17 153 185 445 Cuts at: 7123 325 Size: 1498 Fragments arranged by size: 3515 1498 1255 736 492 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2383 6542 8254 Size: 274 80 33 51 1443 406 4159 1712 Cuts at: 8254 96 Size: 138 Fragments arranged by size: 4159 1712 1443 406 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3715 3747 3954 3979 3986 4011 5571 5603 8256 Size: 32 207 25 7 25 1560 32 2653 Cuts at: 8256 8288 3715 Size: 32 3723 Fragments arranged by size: 3723 2653 1560 207 32 32 32 25 25 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8296 XbaI T'CTAG_A Cuts at: 2026 3355 4096 5632 2026 Size: 1329 741 1536 4690 Fragments arranged by size: 4690 1536 1329 741 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3549 3549 Size: 8296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8296 XmaI C'CCGG_G Cuts at: 1870 5621 1870 Size: 3751 4545 XmnI GAAnn'nnTTC Cuts at: 5539 6425 5539 Size: 886 7410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6104 276 Size: 53 83 186 936 4570 2468 Fragments arranged by size: 4570 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BsiWI BsmBI DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI