(Circular) (Six-base) MAPSORT of: pFN21AE5572.seq Check: 9257 from: 1 to: 6088 pFN21AE5572 6088 bp With 182 enzymes: SgfI * May 22, 2017 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2831 2831 Size: 6088 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2640 3095 3276 4151 5535 5803 1632 Size: 1008 455 181 875 1384 268 1917 Fragments arranged by size: 1917 1384 1008 875 455 268 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 2402 3146 3183 4987 5964 1203 Size: 1199 744 37 1804 977 1327 Fragments arranged by size: 1804 1327 1199 977 744 37 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 3831 5403 3831 Size: 1572 4516 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2121 2261 2611 3044 3397 3623 3775 5884 Size: 628 140 350 433 353 226 152 2109 Cuts at: 5884 1493 Size: 1697 Fragments arranged by size: 2109 1697 628 433 353 350 226 152 140 AseI AT'TA_AT Cuts at: 160 1032 2340 2931 4642 160 Size: 872 1308 591 1711 1606 Fragments arranged by size: 1711 1606 1308 872 591 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2679 3413 1870 Size: 73 736 734 4545 Fragments arranged by size: 4545 736 734 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3327 3360 3327 Size: 33 6055 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 4864 618 Size: 325 217 627 1622 1455 1842 Fragments arranged by size: 1842 1622 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3407 1391 Size: 2016 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3073 3143 928 Size: 971 1174 70 3873 Fragments arranged by size: 3873 1174 971 70 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4279 4313 1903 Size: 34 50 34 2258 34 3678 Fragments arranged by size: 3678 2258 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3355 3980 5190 3355 Size: 625 1210 4253 Fragments arranged by size: 4253 1210 625 BclI T'GATC_A Cuts at: 1548 2022 2101 1548 Size: 474 79 5535 Fragments arranged by size: 5535 474 79 BfrBI ATG'CAT Cuts at: 2753 2753 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 4149 5305 1790 Size: 2359 1156 2573 Fragments arranged by size: 2573 2359 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4748 1239 Size: 3509 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2204 4213 5078 5376 2204 Size: 2009 865 298 2916 Fragments arranged by size: 2916 2009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 2286 6082 2286 Size: 3796 2292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5863 Fragments arranged by size: 5863 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2873 1186 Size: 1687 4401 BseYI C'CCAG_C Cuts at: 3069 3294 5291 5787 3069 Size: 225 1997 496 3370 Fragments arranged by size: 3370 1997 496 225 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4149 4234 5305 4149 Size: 85 1071 4932 Fragments arranged by size: 4932 1071 85 BsmI GAATG_Cn' Cuts at: 2753 3599 3692 2753 Size: 846 93 5149 Fragments arranged by size: 5149 846 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3407 4149 4234 5305 1391 Size: 399 1617 742 85 1071 2174 Fragments arranged by size: 2174 1617 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 2104 3977 5707 2104 Size: 1873 1730 2485 Fragments arranged by size: 2485 1873 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2716 2831 3443 844 Size: 1872 115 612 3489 Fragments arranged by size: 3489 1872 612 115 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2949 3009 3318 4583 4757 65 Size: 1183 1701 60 309 1265 174 1396 Fragments arranged by size: 1701 1396 1265 1183 309 174 60 BsrFI r'CCGG_y Cuts at: 1700 1810 4732 4919 1700 Size: 110 2922 187 2869 Fragments arranged by size: 2922 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2087 96 Size: 1991 4097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 2226 4148 5160 2226 Size: 1922 1012 3154 Fragments arranged by size: 3154 1922 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 2896 4892 2896 Size: 1996 4092 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 3050 3418 3785 4170 4187 5639 5725 5737 Size: 1078 368 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1078 385 368 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5536 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 2529 3604 4398 4418 2529 Size: 1075 794 20 4199 Fragments arranged by size: 4199 1075 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 2313 3392 2313 Size: 1079 5009 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 3535 4424 Size: 54 1425 50 275 18 302 1403 889 Cuts at: 4424 6053 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1403 889 302 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 2536 3070 3297 4018 1353 Size: 804 379 534 227 721 3423 Fragments arranged by size: 3423 804 721 534 379 227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2640 3095 3276 4151 4748 5535 5803 Size: 393 1008 455 181 875 597 787 268 Cuts at: 5803 1239 Size: 1524 Fragments arranged by size: 1524 1008 875 787 597 455 393 268 181 EcoO109I rG'GnC_Cy Cuts at: 3242 3242 Size: 6088 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 EcoRV GAT'ATC Cuts at: 2069 2486 2069 Size: 417 5671 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2198 2198 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 5235 1678 Size: 3557 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2754 2786 4729 Size: 32 135 32 486 32 664 32 1943 Cuts at: 4729 4761 4803 4835 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1943 664 486 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3136 3358 3432 3678 669 Size: 988 281 1198 222 74 246 3079 Fragments arranged by size: 3079 1198 988 281 246 222 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2533 4046 4405 4564 518 Size: 648 858 509 1513 359 159 2042 Fragments arranged by size: 2042 1513 858 648 509 359 159 MspA1I CmG'CkG Cuts at: 1606 3504 4181 5329 5574 1606 Size: 1898 677 1148 245 2120 Fragments arranged by size: 2120 1898 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5536 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NsiI A_TGCA'T Cuts at: 2755 2755 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2406 3150 3187 3446 4991 1132 Size: 97 1177 744 37 259 1545 2229 Fragments arranged by size: 2229 1545 1177 744 259 97 37 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 2402 3146 3183 4987 2402 Size: 744 37 1804 3503 Fragments arranged by size: 3503 1804 744 37 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3242 3242 Size: 6088 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PstI C_TGCA'G Cuts at: 830 3440 830 Size: 2610 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SanDI GG'GwC_CC Cuts at: 3242 3242 Size: 6088 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SexAI A'CCwGG_T Cuts at: 2707 2707 Size: 6088 SfcI C'TryA_G Cuts at: 826 1045 3436 4571 5252 5443 826 Size: 219 2391 1135 681 191 1471 Fragments arranged by size: 2391 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 4192 5093 5355 820 Size: 197 926 240 2009 901 262 1553 Fragments arranged by size: 2009 1553 926 901 262 240 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 2854 4012 5 Size: 47 2802 1158 2081 Fragments arranged by size: 2802 2081 1158 47 StuI AGG'CCT Cuts at: 1517 2043 1517 Size: 526 5562 StyI C'CwwG_G Cuts at: 513 1065 2720 3339 3527 513 Size: 552 1655 619 188 3074 Fragments arranged by size: 3074 1655 619 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2672 3153 4115 4132 4285 4470 4915 Size: 1255 1092 481 962 17 153 185 445 Cuts at: 4915 325 Size: 1498 Fragments arranged by size: 1498 1255 1092 962 481 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2087 2474 2937 Size: 274 80 33 51 1443 110 387 463 Cuts at: 2937 4334 6046 96 Size: 1397 1712 138 Fragments arranged by size: 1712 1443 1397 463 387 274 138 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2192 2224 6048 6080 2192 Size: 32 3824 32 2200 Fragments arranged by size: 3824 2200 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 2545 3035 4217 2545 Size: 490 1182 4416 Fragments arranged by size: 4416 1182 490 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI