(Circular) (Six-base) MAPSORT of: pFN21AE2097.seq Check: 3605 from: 1 to: 7120 pFN21AE2097 7120 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4930 278 Size: 53 83 186 936 3394 2468 Fragments arranged by size: 3394 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4463 1937 Size: 2526 4594 Acc65I G'GTAC_C Cuts at: 4441 4441 Size: 7120 AclI AA'CG_TT Cuts at: 1563 5247 5620 1563 Size: 3684 373 3063 Fragments arranged by size: 3684 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 2273 2288 2304 3055 4119 5183 6567 Size: 558 83 15 16 751 1064 1064 1384 Cuts at: 6567 6835 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1064 1064 751 558 268 83 16 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6923 AflIII A'CryG_T Cuts at: 1203 3366 6019 6996 1203 Size: 2163 2653 977 1327 Fragments arranged by size: 2653 2163 1327 977 AgeI A'CCGG_T Cuts at: 5951 5951 Size: 7120 AhdI GACnn_n'nnGTC Cuts at: 2209 5849 2209 Size: 3640 3480 AleI CACnn'nnGTG Cuts at: 1166 3092 3627 1166 Size: 1926 535 4659 Fragments arranged by size: 4659 1926 535 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7120 AlwNI CAG_nnn'CTG Cuts at: 3653 3664 4863 6435 3653 Size: 11 1199 1572 4338 Fragments arranged by size: 4338 1572 1199 11 ApaLI G'TGCA_C Cuts at: 3085 5177 6333 3085 Size: 2092 1156 3872 Fragments arranged by size: 3872 2092 1156 ApoI r'AATT_y Cuts at: 1493 2265 2992 3405 3805 4429 4655 4807 6916 Size: 772 727 413 400 624 226 152 2109 Cuts at: 6916 1493 Size: 1697 Fragments arranged by size: 2109 1697 772 727 624 413 400 226 152 AseI AT'TA_AT Cuts at: 160 1032 5674 160 Size: 872 4642 1606 Fragments arranged by size: 4642 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 AvaI C'yCGr_G Cuts at: 1870 1943 4445 1870 Size: 73 2502 4545 Fragments arranged by size: 4545 2502 73 BamHI G'GATC_C Cuts at: 3075 4450 4817 3075 Size: 1375 367 5378 Fragments arranged by size: 5378 1375 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4441 5896 618 Size: 325 217 627 2654 1455 1842 Fragments arranged by size: 2654 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3774 4439 1391 Size: 2383 665 4072 Fragments arranged by size: 4072 2383 665 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 2310 2340 2954 3263 3300 928 Size: 971 222 189 30 614 309 37 4748 Fragments arranged by size: 4748 971 614 309 222 189 37 30 BbvCI CC'TCA_GC Cuts at: 2531 3677 2531 Size: 1146 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5311 5345 1903 Size: 34 3374 34 3678 Fragments arranged by size: 3678 3374 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2262 4176 5012 6222 2262 Size: 1914 836 1210 3160 Fragments arranged by size: 3160 1914 1210 836 BclI T'GATC_A Cuts at: 1548 1548 Size: 7120 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5731 136 Size: 107 122 71 631 605 4059 1525 Fragments arranged by size: 4059 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2510 7115 2510 Size: 4605 2515 BlpI GC'TnA_GC Cuts at: 4537 4537 Size: 7120 Bme1580I G_kGCm'C Cuts at: 1790 3089 3480 5181 6337 1790 Size: 1299 391 1701 1156 2573 Fragments arranged by size: 2573 1701 1299 1156 391 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4302 4851 5809 Size: 416 53 83 399 24 2885 549 958 Cuts at: 5809 442 Size: 1753 Fragments arranged by size: 2885 1753 958 549 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2527 3853 5780 1239 Size: 1288 1326 1927 2579 Fragments arranged by size: 2579 1927 1326 1288 Bpu10I CC'TnA_GC Cuts at: 2531 3249 3677 2531 Size: 718 428 5974 Fragments arranged by size: 5974 718 428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2961 5245 6110 6408 2961 Size: 2284 865 298 3673 Fragments arranged by size: 3673 2284 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5783 882 Size: 617 4284 2219 Fragments arranged by size: 4284 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4911 493 Size: 711 3707 2702 Fragments arranged by size: 3707 2702 711 BsaBI GATnn'nnATC Cuts at: 7114 7114 Size: 7120 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4927 5309 5944 275 Size: 53 83 186 936 3394 382 635 1451 Fragments arranged by size: 3394 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5553 5951 6225 6372 6783 5553 Size: 398 274 147 411 5890 Fragments arranged by size: 5890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4248 4278 1178 Size: 30 165 30 2845 30 4020 Fragments arranged by size: 4020 2845 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2631 4019 1186 Size: 1445 1388 4287 Fragments arranged by size: 4287 1445 1388 BseYI C'CCAG_C Cuts at: 2198 6323 6819 2198 Size: 4125 496 2499 Fragments arranged by size: 4125 2499 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3147 3605 4047 1163 Size: 573 1411 458 442 4236 Fragments arranged by size: 4236 1411 573 458 442 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4570 5331 6359 1540 Size: 275 186 2569 761 1028 2301 Fragments arranged by size: 2569 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 2378 3089 5181 5266 6337 2328 Size: 50 711 2092 85 1071 3111 Fragments arranged by size: 3111 2092 1071 711 85 50 BsmI GAATG_Cn' Cuts at: 2429 2758 4631 4724 2429 Size: 329 1873 93 4825 Fragments arranged by size: 4825 1873 329 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2378 3089 3480 3774 4439 5181 Size: 399 538 50 711 391 294 665 742 Cuts at: 5181 5266 6337 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 711 665 538 399 391 294 85 50 BspEI T'CCGG_A Cuts at: 6783 6783 Size: 7120 BspHI T'CATG_A Cuts at: 3124 5009 6739 3124 Size: 1885 1730 3505 Fragments arranged by size: 3505 1885 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4475 844 Size: 3631 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5007 1394 Size: 429 3184 3507 Fragments arranged by size: 3507 3184 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2898 5615 5789 65 Size: 1183 1650 2717 174 1396 Fragments arranged by size: 2717 1650 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5764 5951 1700 Size: 110 3954 187 2869 Fragments arranged by size: 3954 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7120 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7120 BssSI C'ACGA_G Cuts at: 5180 6192 5180 Size: 1012 6108 BstAPI GCAn_nnn'nTGC Cuts at: 794 3161 794 Size: 2367 4753 BstBI TT'CG_AA Cuts at: 5924 5924 Size: 7120 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7120 BstYI r'GATC_y Cuts at: 1972 2510 3075 4450 4817 5202 5219 6671 6757 Size: 538 565 1375 367 385 17 1452 86 Cuts at: 6757 6769 7115 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1375 565 538 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2659 513 Size: 552 1594 4974 Fragments arranged by size: 4974 1594 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6381 Fragments arranged by size: 6381 683 56 BtsI GCAGTG_nn' Cuts at: 3752 4059 4636 5430 5450 3752 Size: 307 577 794 20 5422 Fragments arranged by size: 5422 794 577 307 20 ClaI AT'CG_AT Cuts at: 1554 4356 1554 Size: 2802 4318 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7085 DraI TTT'AAA Cuts at: 2223 3258 3397 3972 4424 2223 Size: 1035 139 575 452 4919 Fragments arranged by size: 4919 1035 575 452 139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6127 809 Size: 507 4811 1802 Fragments arranged by size: 4811 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2662 4567 5456 Size: 54 1425 50 275 18 832 1905 889 Cuts at: 5456 7085 8 Size: 1629 43 Fragments arranged by size: 1905 1629 1425 889 832 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4567 1537 Size: 275 2755 4090 Fragments arranged by size: 4090 2755 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2020 2166 2307 2541 2691 5050 1353 Size: 667 146 141 234 150 2359 3423 Fragments arranged by size: 3423 2359 667 234 150 146 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5707 6081 6227 387 Size: 1064 138 4118 374 146 1280 Fragments arranged by size: 4118 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2273 2288 2304 2527 3055 3853 Size: 393 558 83 15 16 223 528 798 Cuts at: 3853 4119 5183 5780 6567 6835 1239 Size: 266 1064 597 787 268 1524 Fragments arranged by size: 1524 1064 798 787 597 558 528 393 268 266 223 83 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 2734 3059 2734 Size: 325 6795 EcoO109I rG'GnC_Cy Cuts at: 3052 3558 3614 3052 Size: 506 56 6558 Fragments arranged by size: 6558 506 56 EcoRI G'AATT_C Cuts at: 3805 4429 4807 3805 Size: 624 378 6118 Fragments arranged by size: 6118 624 378 EcoRV GAT'ATC Cuts at: 4084 4084 Size: 7120 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3085 3605 3085 Size: 520 6600 FspI TGC'GCA Cuts at: 5626 5626 Size: 7120 HaeII r_GCGC'y Cuts at: 1678 6267 1678 Size: 4589 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2502 2534 3106 Size: 32 135 32 623 32 275 32 572 Cuts at: 3106 3138 3491 3523 4288 4320 5761 5793 5835 Size: 32 353 32 765 32 1441 32 42 Cuts at: 5835 5867 1373 Size: 32 2626 Fragments arranged by size: 2626 1441 765 623 572 353 275 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4464 4710 669 Size: 988 281 2526 246 3079 Fragments arranged by size: 3079 2526 988 281 246 HindIII A'AGCT_T Cuts at: 748 2285 748 Size: 1537 5583 HpaI GTT'AAC Cuts at: 4710 4710 Size: 7120 KpnI G_GTAC'C Cuts at: 4445 4445 Size: 7120 MfeI C'AATT_G Cuts at: 2778 4719 2778 Size: 1941 5179 MluI A'CGCG_T Cuts at: 6996 6996 Size: 7120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6234 6418 1305 Size: 4929 184 2007 Fragments arranged by size: 4929 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5298 Fragments arranged by size: 5298 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2422 2724 2730 3092 3627 3860 3904 Size: 648 1256 302 6 362 535 233 44 Cuts at: 3904 4118 5078 5437 5596 518 Size: 214 960 359 159 2042 Fragments arranged by size: 2042 1256 960 648 535 362 359 302 233 214 159 44 6 MspA1I CmG'CkG Cuts at: 1606 2293 2530 2661 4536 5213 6361 6606 1606 Size: 687 237 131 1875 677 1148 245 2120 Fragments arranged by size: 2120 1875 1148 687 677 245 237 131 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6568 NdeI CA'TA_TG Cuts at: 387 3157 3841 3901 3949 4226 387 Size: 2770 684 60 48 277 3281 Fragments arranged by size: 3281 2770 684 277 60 48 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7120 NotI GC'GGCC_GC Cuts at: 4567 4567 Size: 7120 NspI r_CATG'y Cuts at: 1132 1229 3370 4478 6023 1132 Size: 97 2141 1108 1545 2229 Fragments arranged by size: 2229 2141 1545 1108 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7120 PciI A'CATG_T Cuts at: 3366 6019 3366 Size: 2653 4467 PflMI CCAn_nnn'nTGG Cuts at: 1108 3048 4196 1108 Size: 1940 1148 4032 Fragments arranged by size: 4032 1940 1148 PfoI T'CCnGG_A Cuts at: 4124 4381 4124 Size: 257 6863 PmeI GTTT'AAAC Cuts at: 4424 4424 Size: 7120 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5189 5221 6702 6734 5189 Size: 32 1481 32 5575 Fragments arranged by size: 5575 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3052 3558 3052 Size: 506 6614 PshAI GACnn'nnGTC Cuts at: 1613 3834 1613 Size: 2221 4899 PsiI TTA'TAA Cuts at: 2154 4690 2154 Size: 2536 4584 PstI C_TGCA'G Cuts at: 830 2298 3241 4472 830 Size: 1468 943 1231 3478 Fragments arranged by size: 3478 1468 1231 943 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7120 PvuII CAG'CTG Cuts at: 2293 2530 2293 Size: 237 6883 SacII CC_GC'GG Cuts at: 2662 2662 Size: 7120 SalI G'TCGA_C Cuts at: 1936 4462 1936 Size: 2526 4594 SapI GCTCTTCn'nnn_ Cuts at: 2020 2307 2020 Size: 287 6833 SbfI CC_TGCA'GG Cuts at: 4472 4472 Size: 7120 ScaI AGT'ACT Cuts at: 2389 3965 5368 7080 2389 Size: 1576 1403 1712 2429 Fragments arranged by size: 2429 1712 1576 1403 SexAI A'CCwGG_T Cuts at: 2440 2440 Size: 7120 SfcI C'TryA_G Cuts at: 826 1045 2294 2383 2653 3237 3645 4468 5603 Size: 219 1249 89 270 584 408 823 1135 Cuts at: 5603 6284 6475 826 Size: 681 191 1471 Fragments arranged by size: 1471 1249 1135 823 681 584 408 270 219 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7120 SmaI CCC'GGG Cuts at: 1872 4447 1872 Size: 2575 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2976 5224 6125 6387 820 Size: 197 926 1033 2248 901 262 1553 Fragments arranged by size: 2248 1553 1033 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4911 493 Size: 4418 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7120 SphI G_CATG'C Cuts at: 1132 4478 1132 Size: 3346 3774 SspI AAT'ATT Cuts at: 5 52 2164 2925 5044 5 Size: 47 2112 761 2119 2081 Fragments arranged by size: 2119 2112 2081 761 47 StuI AGG'CCT Cuts at: 1517 2906 1517 Size: 1389 5731 StyI C'CwwG_G Cuts at: 513 1065 4559 513 Size: 552 3494 3074 Fragments arranged by size: 3494 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5147 5164 5317 5502 5947 325 Size: 1255 3567 17 153 185 445 1498 Fragments arranged by size: 3567 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2387 2838 3963 Size: 274 80 33 51 1443 410 451 1125 Cuts at: 3963 5366 7078 96 Size: 1403 1712 138 Fragments arranged by size: 1712 1443 1403 1125 451 410 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4248 4280 7080 7112 4248 Size: 32 2800 32 4256 Fragments arranged by size: 4256 2800 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7120 XbaI T'CTAG_A Cuts at: 4456 4456 Size: 7120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 3212 2086 Size: 1126 5994 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7120 XmaI C'CCGG_G Cuts at: 1870 4445 1870 Size: 2575 4545 XmnI GAAnn'nnTTC Cuts at: 2721 3665 5249 2721 Size: 944 1584 4592 Fragments arranged by size: 4592 1584 944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4928 276 Size: 53 83 186 936 3394 2468 Fragments arranged by size: 3394 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI