################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoT22I FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AluI 7 AGCT AG^CT BciVI 445 GTATCC GTATCC(6/5) BpmI 345 CTCCAG CTGGAG(16/14) BsiEI 313 CGGCCG CG[GA][CT]^CG BsmFI 15 GGGAC GGGAC(10/14) DdeI 123 CTCAG C^T[ACGT]AG DpnII 382 GATC ^GATC DraII 520 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 17 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 313 CGGCCG [CT]^GGCC[GA] EagI 313 CGGCCG C^GGCCG EclXI 313 CGGCCG C^GGCCG Eco52I 313 CGGCCG C^GGCCG EcoNI 319 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 520 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 127 GATATC GAT^ATC EcoT14I 199 CCAAGG C^C[AT][AT]GG FauI 294 CCCGC CCCGC(4/6) HapII 239 CCGG C^CGG HgaI 287 GACGC GACGC(5/10) HinfI 171 GAATC G^A[ACGT]TC HpaII 239 CCGG C^CGG MaeII 394 ACGT A^CGT MboI 382 GATC ^GATC MspA1I 180 CAGCGG C[AC]G^C[GT]G MspI 239 CCGG C^CGG NdeII 382 GATC ^GATC NlaIII 526 CATG CATG^ NlaIV 521 GGACCC GG[ACGT]^[ACGT]CC PpuMI 520 AGGACCC [GA]G^G[AT]CC[CT] PstI 320 CTGCAG CTGCA^G Sau3AI 382 GATC ^GATC SbfI 319 CCTGCAGG CCTGCA^GG SfaNI 85 GATGC GCATC(5/9) SfcI 320 CTGCAG C^T[GA][CT]AG Sse8387I 319 CCTGCAGG CCTGCA^GG StyI 199 CCAAGG C^C[AT][AT]GG TfiI 171 GAATC G^A[AT]TC Tsp45I 460 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaI 56 CCCGAG C^[CT]CG[GA]G 166 CCCGAG AvaII 273 GGACC G^G[AT]CC 521 GGACC BbvI 475 GCAGC GCAGC(8/12) 478 GCAGC BsmAI 68 GAGAC GTCTC(1/5) 435 GTCTC BsoBI 56 CCCGAG C^[CT]CG[GA]G 166 CCCGAG BsrI 347 CCAGT ACTGG(1/-1) 482 CCAGT Cac8I 51 GCACGC GC[ACGT]^[ACGT]GC 318 GCCTGC DsaI 310 CCACGG C^C[GA][CT]GG 449 CCGTGG HphI 459 GGTGA GGTGA(8/7) 384 TCACC MaeIII 257 GTTAC ^GT[ACGT]AC 460 GTGAC TseI 475 GCAGC G^C[AT]GC 478 GCAGC Tsp509I 235 AATT ^AATT 511 AATT TspRI 246 CAGTG CA[GC]TG[ACGT][ACGT]^ 464 CACTG XcmI 387 CCATTCAACGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 440 CCATCGTATCCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI BsaJI BsiYI BslI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI Sau96I ScrFI