(Linear) (Six-base) MAPSORT of: KIEE1949.seq Check: 4927 from: 1 to: 1512 KIEE1949.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 585 678 1512 Size: 585 93 834 Fragments arranged by size: 834 585 93 AfeI AGC'GCT Cuts at: 0 777 1512 Size: 777 735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 585 1512 Size: 585 927 AlwNI CAG_nnn'CTG Cuts at: 0 249 831 1052 1512 Size: 249 582 221 460 Fragments arranged by size: 582 460 249 221 ApaLI G'TGCA_C Cuts at: 0 560 1512 Size: 560 952 ApoI r'AATT_y Cuts at: 0 482 1512 Size: 482 1030 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1144 1177 1512 Size: 1144 33 335 Fragments arranged by size: 1144 335 33 BanII G_rGCy'C Cuts at: 0 263 644 1135 1263 1439 1512 Size: 263 381 491 128 176 73 Fragments arranged by size: 491 381 263 176 128 73 BbsI GAAGACnn'nnnn_ Cuts at: 0 192 1512 Size: 192 1320 BbvCI CC'TCA_GC Cuts at: 0 419 1512 Size: 419 1093 BglI GCCn_nnn'nGGC Cuts at: 0 508 889 1512 Size: 508 381 623 Fragments arranged by size: 623 508 381 BlpI GC'TnA_GC Cuts at: 0 90 1512 Size: 90 1422 Bme1580I G_kGCm'C Cuts at: 0 130 564 825 1512 Size: 130 434 261 687 Fragments arranged by size: 687 434 261 130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 376 1167 1347 1491 1512 Size: 373 3 791 180 144 21 Fragments arranged by size: 791 373 180 144 21 3 Bpu10I CC'TnA_GC Cuts at: 0 419 1512 Size: 419 1093 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 448 475 1512 Size: 145 303 27 1037 Fragments arranged by size: 1037 303 145 27 BsaHI Gr'CG_yC Cuts at: 0 368 1512 Size: 368 1144 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 859 889 1142 1172 1512 Size: 859 30 253 30 340 Fragments arranged by size: 859 340 253 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 27 30 357 425 1512 Size: 27 3 327 68 1087 Fragments arranged by size: 1087 327 68 27 3 BseYI C'CCAG_C Cuts at: 0 242 267 660 1104 1134 1140 1512 Size: 242 25 393 444 30 6 372 Fragments arranged by size: 444 393 372 242 30 25 6 BsiEI CG_ry'CG Cuts at: 0 1428 1512 Size: 1428 84 BsiHKAI G_wGCw'C Cuts at: 0 263 290 564 644 718 1198 1512 Size: 263 27 274 80 74 480 314 Fragments arranged by size: 480 314 274 263 80 74 27 BsmI GAATG_Cn' Cuts at: 0 285 1512 Size: 285 1227 Bsp1286I G_dGCh'C Cuts at: 0 130 263 290 564 644 718 825 1135 Size: 130 133 27 274 80 74 107 310 Cuts at: 1135 1198 1263 1439 1512 Size: 63 65 176 73 Fragments arranged by size: 310 274 176 133 130 107 80 74 73 65 63 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 68 585 678 1512 Size: 68 517 93 834 Fragments arranged by size: 834 517 93 68 BsrBI CCG'CTC Cuts at: 0 1388 1512 Size: 1388 124 BsrGI T'GTAC_A Cuts at: 0 875 1512 Size: 875 637 BssSI C'ACGA_G Cuts at: 0 1063 1512 Size: 1063 449 BstAPI GCAn_nnn'nTGC Cuts at: 0 429 1512 Size: 429 1083 BstXI CCAn_nnnn'nTGG Cuts at: 0 79 237 904 1512 Size: 79 158 667 608 Fragments arranged by size: 667 608 158 79 BstYI r'GATC_y Cuts at: 0 449 1512 Size: 449 1063 BtgI C'CryG_G Cuts at: 0 497 816 897 1482 1512 Size: 497 319 81 585 30 Fragments arranged by size: 585 497 319 81 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 783 946 1512 Size: 783 163 566 Fragments arranged by size: 783 566 163 BtsI GCAGTG_nn' Cuts at: 0 980 1512 Size: 980 532 EaeI y'GGCC_r Cuts at: 0 500 1512 Size: 500 1012 EarI CTCTTCn'nnn_ Cuts at: 0 649 1387 1512 Size: 649 738 125 Fragments arranged by size: 738 649 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 738 1512 Size: 738 774 EcoICRI GAG'CTC Cuts at: 0 261 642 1512 Size: 261 381 870 Fragments arranged by size: 870 381 261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 376 1167 1347 1491 1512 Size: 373 3 791 180 144 21 Fragments arranged by size: 791 373 180 144 21 3 EcoO109I rG'GnC_Cy Cuts at: 0 882 1512 Size: 882 630 HaeII r_GCGC'y Cuts at: 0 779 1190 1512 Size: 779 411 322 Fragments arranged by size: 779 411 322 HincII GTy'rAC Cuts at: 0 1254 1512 Size: 1254 258 MfeI C'AATT_G Cuts at: 0 400 1512 Size: 400 1112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 122 845 1512 Size: 122 723 667 Fragments arranged by size: 723 667 122 MslI CAynn'nnrTG Cuts at: 0 567 1512 Size: 567 945 MspA1I CmG'CkG Cuts at: 0 155 246 276 429 606 660 831 1104 Size: 155 91 30 153 177 54 171 273 Cuts at: 1104 1144 1512 Size: 40 368 Fragments arranged by size: 368 273 177 171 155 153 91 54 40 30 NcoI C'CATG_G Cuts at: 0 497 816 897 1482 1512 Size: 497 319 81 585 30 Fragments arranged by size: 585 497 319 81 30 PasI CC'CwG_GG Cuts at: 0 1239 1512 Size: 1239 273 PfoI T'CCnGG_A Cuts at: 0 350 1512 Size: 350 1162 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1081 1113 1512 Size: 1081 32 399 Fragments arranged by size: 1081 399 32 PstI C_TGCA'G Cuts at: 0 29 323 1149 1331 1512 Size: 29 294 826 182 181 Fragments arranged by size: 826 294 182 181 29 PvuII CAG'CTG Cuts at: 0 155 276 429 606 660 1144 1512 Size: 155 121 153 177 54 484 368 Fragments arranged by size: 484 368 177 155 153 121 54 SacI G_AGCT'C Cuts at: 0 263 644 1512 Size: 263 381 868 Fragments arranged by size: 868 381 263 SapI GCTCTTCn'nnn_ Cuts at: 0 649 1512 Size: 649 863 SfcI C'TryA_G Cuts at: 0 25 319 1145 1327 1512 Size: 25 294 826 182 185 Fragments arranged by size: 826 294 185 182 25 SmlI C'TyrA_G Cuts at: 0 160 454 463 1512 Size: 160 294 9 1049 Fragments arranged by size: 1049 294 160 9 StuI AGG'CCT Cuts at: 0 325 1379 1512 Size: 325 1054 133 Fragments arranged by size: 1054 325 133 StyI C'CwwG_G Cuts at: 0 371 497 816 897 1482 1512 Size: 371 126 319 81 585 30 Fragments arranged by size: 585 371 319 126 81 30 TatI w'GTAC_w Cuts at: 0 875 1512 Size: 875 637 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 687 719 1142 1174 1255 1287 1512 Size: 687 32 423 32 81 32 225 Fragments arranged by size: 687 423 225 81 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 152 504 1512 Size: 152 352 1008 Fragments arranged by size: 1008 352 152 Enzymes that do cut: AarI AfeI AlfI AlwNI ApaLI ApoI BaeI BanII BbsI BbvCI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII HincII MfeI MmeI MslI MspA1I NcoI PasI PfoI PsrI PstI PvuII SacI SapI SfcI SmlI StuI StyI TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI