(Circular) (Six-base) MAPSORT of: pFN21AE9561.seq Check: 1142 from: 1 to: 5809 pFN21AE9561 5809 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2310 2310 Size: 5809 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2874 3152 1937 Size: 937 278 4594 Fragments arranged by size: 4594 937 278 Acc65I G'GTAC_C Cuts at: 2364 3130 2364 Size: 766 5043 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2782 3872 5256 5524 1632 Size: 1150 1090 1384 268 1917 Fragments arranged by size: 1917 1384 1150 1090 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2312 1184 Size: 1128 4681 AlwNI CAG_nnn'CTG Cuts at: 2066 3552 5124 2066 Size: 1486 1572 2751 Fragments arranged by size: 2751 1572 1486 ApaI G_GGCC'C Cuts at: 2281 2993 3056 2281 Size: 712 63 5034 Fragments arranged by size: 5034 712 63 ApaLI G'TGCA_C Cuts at: 2297 3866 5022 2297 Size: 1569 1156 3084 Fragments arranged by size: 3084 1569 1156 ApoI r'AATT_y Cuts at: 1493 3118 3344 3496 5605 1493 Size: 1625 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 152 AscI GG'CGCG_CC Cuts at: 2707 2707 Size: 5809 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 2143 2986 3096 3134 1870 Size: 73 200 843 110 38 4545 Fragments arranged by size: 4545 843 200 110 73 38 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2364 2379 2467 2489 3130 Size: 325 217 627 577 15 88 22 641 Cuts at: 3130 4585 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 641 627 577 325 217 88 22 15 BanII G_rGCy'C Cuts at: 1391 2281 2517 2993 3056 3063 3128 1391 Size: 890 236 476 63 7 65 4072 Fragments arranged by size: 4072 890 476 236 65 63 7 BbeI G_GCGC'C Cuts at: 2471 2471 Size: 5809 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BbvCI CC'TCA_GC Cuts at: 2477 2477 Size: 5809 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4911 3701 Size: 1210 4599 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2717 2997 4420 Size: 107 122 71 631 605 1045 280 1423 Cuts at: 4420 136 Size: 1525 Fragments arranged by size: 1525 1423 1045 631 605 280 122 107 71 BglII A'GATC_T Cuts at: 5804 5804 Size: 5809 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 2099 2235 2281 2301 2526 2953 2993 3056 Size: 309 136 46 20 225 427 40 63 Cuts at: 3056 3870 5026 1790 Size: 814 1156 2573 Fragments arranged by size: 2573 1156 814 427 309 225 136 63 46 40 20 BmgBI CAC'GTC Cuts at: 1318 2560 1318 Size: 1242 4567 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3540 4498 442 Size: 416 53 83 399 24 2123 958 1753 Fragments arranged by size: 2123 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 4469 1239 Size: 1292 1938 2579 Fragments arranged by size: 2579 1938 1292 Bpu10I CC'TnA_GC Cuts at: 2477 2477 Size: 5809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3934 4799 5097 3934 Size: 865 298 4646 Fragments arranged by size: 4646 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4472 882 Size: 617 2973 2219 Fragments arranged by size: 2973 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 5803 5803 Size: 5809 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2468 2938 3012 3616 Size: 53 83 186 936 935 470 74 604 Cuts at: 3616 3998 4633 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 935 635 604 470 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 3103 4242 4640 4914 5061 5472 3103 Size: 1139 398 274 147 411 3440 Fragments arranged by size: 3440 1139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2837 2867 1178 Size: 30 165 30 1434 30 4120 Fragments arranged by size: 4120 1434 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5809 BseYI C'CCAG_C Cuts at: 2092 2745 5012 5508 2092 Size: 653 2267 496 2393 Fragments arranged by size: 2393 2267 653 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2673 2788 2932 3259 4020 5048 Size: 275 186 672 115 144 327 761 1028 Cuts at: 5048 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 672 327 275 186 144 115 BsiHKAI G_wGCw'C Cuts at: 2130 2301 2517 3870 3955 5026 2130 Size: 171 216 1353 85 1071 2913 Fragments arranged by size: 2913 1353 1071 216 171 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 BsmBI CGTCTCn'nnnn_ Cuts at: 3003 3003 Size: 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2130 2235 2281 2301 2517 2526 Size: 399 309 31 105 46 20 216 9 Cuts at: 2526 2953 2993 3056 3063 3128 3870 3955 5026 Size: 427 40 63 7 65 742 85 1071 Cuts at: 5026 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 427 399 309 216 105 85 65 63 46 40 31 20 9 7 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 2592 3164 844 Size: 1466 282 572 3489 Fragments arranged by size: 3489 1466 572 282 BsrBI CCG'CTC Cuts at: 1394 1823 2869 3696 1394 Size: 429 1046 827 3507 Fragments arranged by size: 3507 1046 827 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2113 4304 4478 65 Size: 1183 865 2191 174 1396 Fragments arranged by size: 2191 1396 1183 865 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2576 4453 4640 1700 Size: 110 766 1877 187 2869 Fragments arranged by size: 2869 1877 766 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 2395 2505 2540 2707 2901 2903 1925 Size: 470 110 35 167 194 2 4831 Fragments arranged by size: 4831 470 194 167 110 35 2 BssSI C'ACGA_G Cuts at: 3869 4881 3869 Size: 1012 4797 BstAPI GCAn_nnn'nTGC Cuts at: 794 2386 794 Size: 1592 4217 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstYI r'GATC_y Cuts at: 1972 3139 3506 3891 3908 5360 5446 5458 5804 Size: 1167 367 385 17 1452 86 12 346 Cuts at: 5804 1972 Size: 1977 Fragments arranged by size: 1977 1452 1167 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2172 2355 2592 2646 2655 Size: 552 939 51 117 183 237 54 9 Cuts at: 2655 3079 513 Size: 424 3243 Fragments arranged by size: 3243 939 552 424 237 183 117 54 51 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2130 2436 2833 3325 4119 4139 2067 Size: 63 306 397 492 794 20 3737 Fragments arranged by size: 3737 794 492 397 306 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 2562 2670 Size: 54 1425 50 275 18 540 192 108 Cuts at: 2670 2724 2785 2805 2921 2929 3046 3256 4145 Size: 54 61 20 116 8 117 210 889 Cuts at: 4145 5774 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 540 275 210 192 117 116 108 61 54 54 50 43 20 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2670 2785 2929 3256 1537 Size: 275 858 115 144 327 4090 Fragments arranged by size: 4090 858 327 275 144 115 EarI CTCTTCn'nnn_ Cuts at: 1353 2465 3739 1353 Size: 1112 1274 3423 Fragments arranged by size: 3423 1274 1112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2698 2900 2963 3099 4396 4770 Size: 1064 138 1109 202 63 136 1297 374 Cuts at: 4770 4916 387 Size: 146 1280 Fragments arranged by size: 1297 1280 1109 1064 374 202 146 138 136 63 EcoICRI GAG'CTC Cuts at: 2515 2515 Size: 5809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2531 2782 3872 4469 5256 5524 1239 Size: 393 899 251 1090 597 787 268 1524 Fragments arranged by size: 1524 1090 899 787 597 393 268 251 EcoO109I rG'GnC_Cy Cuts at: 2277 2278 2651 3052 3071 2277 Size: 1 373 401 19 5015 Fragments arranged by size: 5015 401 373 19 1 EcoRI G'AATT_C Cuts at: 3118 3496 3118 Size: 378 5431 FspI TGC'GCA Cuts at: 2405 4315 2405 Size: 1910 3899 HaeII r_GCGC'y Cuts at: 1678 2459 2471 4956 1678 Size: 781 12 2485 2531 Fragments arranged by size: 2531 2485 781 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4450 4482 4524 4556 1373 Size: 32 135 32 2878 32 42 32 2626 Fragments arranged by size: 2878 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3153 3399 669 Size: 988 281 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KasI G'GCGC_C Cuts at: 2467 2467 Size: 5809 KpnI G_GTAC'C Cuts at: 2368 3134 2368 Size: 766 5043 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4923 5107 1305 Size: 3618 184 2007 Fragments arranged by size: 3618 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 10 Size: 54 1425 343 732 3255 Fragments arranged by size: 3255 1425 732 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3767 4126 4285 518 Size: 648 2601 359 159 2042 Fragments arranged by size: 2601 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2386 2657 3225 3902 5050 5295 1606 Size: 780 271 568 677 1148 245 2120 Fragments arranged by size: 2120 1148 780 677 568 271 245 NaeI GCC'GGC Cuts at: 2578 2578 Size: 5809 NarI GG'CG_CC Cuts at: 2468 2468 Size: 5809 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4318 Fragments arranged by size: 4318 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NgoMIV G'CCGG_C Cuts at: 2576 2576 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 2929 3256 2929 Size: 327 5482 NspI r_CATG'y Cuts at: 1132 1229 3167 4712 1132 Size: 97 1938 1545 2229 Fragments arranged by size: 2229 1938 1545 97 PasI CC'CwG_GG Cuts at: 1348 2039 3063 1348 Size: 691 1024 4094 Fragments arranged by size: 4094 1024 691 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5809 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2651 3071 2651 Size: 420 5389 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PspOMI G'GGCC_C Cuts at: 2277 2989 3052 2277 Size: 712 63 5034 Fragments arranged by size: 5034 712 63 PstI C_TGCA'G Cuts at: 830 2136 3161 830 Size: 1306 1025 3478 Fragments arranged by size: 3478 1306 1025 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 SacI G_AGCT'C Cuts at: 2517 2517 Size: 5809 SacII CC_GC'GG Cuts at: 2658 2658 Size: 5809 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SanDI GG'GwC_CC Cuts at: 2651 3071 2651 Size: 420 5389 SapI GCTCTTCn'nnn_ Cuts at: 2465 2465 Size: 5809 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SfcI C'TryA_G Cuts at: 826 1045 2132 3157 4292 4973 5164 826 Size: 219 1087 1025 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 1025 681 219 191 SfoI GGC'GCC Cuts at: 2469 2469 Size: 5809 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 3136 1872 Size: 1264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3913 4814 5076 820 Size: 197 926 1970 901 262 1553 Fragments arranged by size: 1970 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5809 StyI C'CwwG_G Cuts at: 513 1065 2004 3248 513 Size: 552 939 1244 3074 Fragments arranged by size: 3074 1244 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3836 3853 4006 4191 4636 325 Size: 1255 2256 17 153 185 445 1498 Fragments arranged by size: 2256 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4055 5767 96 Size: 274 80 33 51 1443 2078 1712 138 Fragments arranged by size: 2078 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5769 5801 5769 Size: 32 5777 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 2719 2254 Size: 465 5344 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 3134 1870 Size: 1264 4545 XmnI GAAnn'nnTTC Cuts at: 3938 3938 Size: 5809 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI