################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MseI MslI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 361 GACGTC GACGT^C AccI 817 GTCTAC GT^[AC][GT]AC AspI 811 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 182 TGGCCA TGG^CCA BamHI 104 GGATCC G^GATCC BanI 391 GGCACC G^G[CT][GA]CC BbvCI 21 CCTCAGC CCTCAGC(-5/-2) BfaI 577 CTAG C^TAG BglI 254 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 164 AGATCT A^GATCT BlpI 600 GCTCAGC GC^T[ACGT]AGC Bpu1102I 600 GCTCAGC GC^T[ACGT]AGC BsaI 406 GAGACC GGTCTC(1/5) BsiEI 413 CGGCCG CG[GA][CT]^CG BsmAI 406 GAGAC GTCTC(1/5) BspHI 776 TCATGA T^CATGA BsrFI 824 ACCGGC [GA]^CCGG[CT] BsrI 479 ACTGG ACTGG(1/-1) BssHII 689 GCGCGC G^CGCGC BstAPI 522 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 173 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 600 GCTCAGC GC^T[ACGT]AGC Cfr10I 824 ACCGGC [GA]^CCGG[CT] DraIII 523 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 413 CGGCCG C^GGCCG EclXI 413 CGGCCG C^GGCCG Eco52I 413 CGGCCG C^GGCCG EcoNI 576 CCTAGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 203 CCATGG C^C[AT][AT]GG HapII 825 CCGG C^CGG HindIII 351 AAGCTT A^AGCTT HpaII 825 CCGG C^CGG KspI 410 CCGCGG CCGC^GG MaeI 577 CTAG C^TAG MaeIII 506 GTAAC ^GT[ACGT]AC MluNI 182 TGGCCA TGG^CCA MscI 182 TGGCCA TGG^CCA MspI 825 CCGG C^CGG NcoI 203 CCATGG C^CATGG PaeR7I 763 CTCGAG C^TCGAG PflFI 811 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 565 GACTC GAGTC(4/5) RcaI 776 TCATGA T^CATGA SacII 410 CCGCGG CCGC^GG ScaI 35 AGTACT AGT^ACT StyI 203 CCATGG C^C[AT][AT]GG TspRI 636 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 811 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 763 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 361 GACGTC G[GA]^CG[CT]C 751 GGCGTC AfaI 36 GTAC GT^AC 387 GTAC AlwI 105 GATCC GGATC(4/5) 104 GGATC ApaI 233 GGGCCC GGGCC^C 335 GGGCCC AspHI 244 GAGCTC G[AT]GC[AT]^C 766 GAGCTC BpmI 700 CTCCAG CTGGAG(16/14) 343 CTGGAG BsaHI 361 GACGTC G[GA]^CG[CT]C 751 GGCGTC BseRI 403 GAGGAG GAGGAG(10/8) 418 GAGGAG BsiHKAI 244 GAGCTC G[AT]GC[AT]^C 766 GAGCTC BsiYI 326 CCACTCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 576 CCTAGATAAGG BslI 326 CCACTCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 576 CCTAGATAAGG BstYI 104 GGATCC [GA]^GATC[CT] 164 AGATCT DsaI 203 CCATGG C^C[GA][CT]GG 410 CCGCGG EaeI 182 TGGCCA [CT]^GGCC[GA] 413 CGGCCG EarI 43 CTCTTC CTCTTC(1/4) 58 CTCTTC HgaI 780 GACGC GACGC(5/10) 752 GCGTC Hin1I 361 GACGTC G[GA]^CG[CT]C 751 GGCGTC Ksp632I 43 CTCTTC CTCTTC(1/4) 58 CTCTTC MaeII 362 ACGT A^CGT 815 ACGT MflI 104 GGATCC [GA]^GATC[CT] 164 AGATCT NlaIII 204 CATG CATG^ 777 CATG PstI 160 CTGCAG CTGCA^G 659 CTGCAG PvuII 340 CAGCTG CAG^CTG 445 CAGCTG RsaI 36 GTAC GT^AC 387 GTAC SacI 244 GAGCTC GAGCT^C 766 GAGCTC SfcI 160 CTGCAG C^T[GA][CT]AG 659 CTGCAG SmlI 238 CTCAAG C^T[CT][GA]AG 763 CTCGAG TfiI 194 GAATC G^A[AT]TC 314 GAATC XhoII 104 GGATCC [GA]^GATC[CT] 164 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AccII AciI AluI AvaI AvaII BanII BbvI BmyI BsaJI BsgI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MboI MboII MnlI MspA1I MvaI MvnI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I