(Circular) (Six-base) MAPSORT of: pFN21AE2737.seq Check: 3035 from: 1 to: 5548 pFN21AE2737 5548 bp With 182 enzymes: SgfI * December 20, 2018 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2370 3358 278 Size: 53 83 186 936 834 988 2468 Fragments arranged by size: 2468 988 936 834 186 83 53 AccI GT'mk_AC Cuts at: 1937 2823 2891 1937 Size: 886 68 4594 Fragments arranged by size: 4594 886 68 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 4995 5263 1632 Size: 1979 1384 268 1917 Fragments arranged by size: 1979 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2668 1184 Size: 1484 4064 AlwNI CAG_nnn'CTG Cuts at: 3291 4863 3291 Size: 1572 3976 ApaI G_GGCC'C Cuts at: 2242 2344 2242 Size: 102 5446 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2857 3083 3235 5344 1493 Size: 1364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2405 2768 2774 2873 1870 Size: 73 462 363 6 99 4545 Fragments arranged by size: 4545 462 363 99 73 6 BamHI G'GATC_C Cuts at: 2109 2878 3245 2109 Size: 769 367 4412 Fragments arranged by size: 4412 769 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 2869 4324 618 Size: 325 217 627 609 473 1455 1842 Fragments arranged by size: 1842 1455 627 609 473 325 217 BanII G_rGCy'C Cuts at: 1391 2242 2253 2344 2775 2867 1391 Size: 851 11 91 431 92 4072 Fragments arranged by size: 4072 851 431 92 91 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4577 BbvCI CC'TCA_GC Cuts at: 2027 2027 Size: 5548 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2265 4159 136 Size: 107 122 71 631 605 593 1894 1525 Fragments arranged by size: 1894 1525 631 605 593 122 107 71 BglII A'GATC_T Cuts at: 2169 5543 2169 Size: 3374 2174 BlpI GC'TnA_GC Cuts at: 2606 2965 2606 Size: 359 5189 Bme1580I G_kGCm'C Cuts at: 1790 2242 2344 2538 3609 4765 1790 Size: 452 102 194 1071 1156 2573 Fragments arranged by size: 2573 1156 1071 452 194 102 BmgBI CAC'GTC Cuts at: 1318 2821 1318 Size: 1503 4045 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 2690 4208 1239 Size: 1130 321 1518 2579 Fragments arranged by size: 2579 1518 1130 321 Bpu10I CC'TnA_GC Cuts at: 2027 2027 Size: 5548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 3673 4538 4836 2228 Size: 1445 865 298 2940 Fragments arranged by size: 2940 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2405 4211 882 Size: 617 906 1806 2219 Fragments arranged by size: 2219 1806 906 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 2757 3355 3737 Size: 53 83 186 936 834 390 598 382 Cuts at: 3737 4372 275 Size: 635 1451 Fragments arranged by size: 1451 936 834 635 598 390 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 2438 1186 Size: 1237 15 4296 Fragments arranged by size: 4296 1237 15 BseYI C'CCAG_C Cuts at: 2343 2430 2601 4751 5247 2343 Size: 87 171 2150 496 2644 Fragments arranged by size: 2644 2150 496 171 87 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2462 2664 2702 2824 1163 Size: 573 726 202 38 122 3887 Fragments arranged by size: 3887 726 573 202 122 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2421 2998 3759 4787 1540 Size: 275 186 420 577 761 1028 2301 Fragments arranged by size: 2301 1028 761 577 420 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 2775 3609 3694 4765 2253 Size: 522 834 85 1071 3036 Fragments arranged by size: 3036 1071 834 522 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2242 2253 2344 2538 2775 2867 3609 Size: 399 452 11 91 194 237 92 742 Cuts at: 3609 3694 4765 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 399 237 194 92 91 85 11 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 2781 3437 5167 2781 Size: 656 1730 3162 Fragments arranged by size: 3162 1730 656 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 844 Size: 2059 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4043 4217 65 Size: 1183 2795 174 1396 Fragments arranged by size: 2795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2829 4192 4379 1700 Size: 110 1019 1363 187 2869 Fragments arranged by size: 2869 1363 1019 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 2694 1925 Size: 769 4779 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 2533 794 Size: 1739 3809 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstXI CCAn_nnnn'nTGG Cuts at: 2185 2185 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2109 2169 2878 3245 3630 3647 5099 5185 Size: 137 60 709 367 385 17 1452 86 Cuts at: 5185 5197 5543 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 709 385 367 346 137 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2208 2415 513 Size: 552 1143 207 3646 Fragments arranged by size: 3646 1143 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2200 487 Size: 683 56 974 3835 Fragments arranged by size: 3835 974 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3858 3878 3064 Size: 794 20 4734 Fragments arranged by size: 4734 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DraIII CAC_nnn'GTG Cuts at: 2533 2533 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2418 2995 Size: 54 1425 50 275 18 357 231 577 Cuts at: 2995 3884 5513 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 577 357 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2418 2995 1537 Size: 275 606 577 4090 Fragments arranged by size: 4090 606 577 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2069 3478 1353 Size: 701 15 1409 3423 Fragments arranged by size: 3423 1409 701 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2251 2773 2251 Size: 522 5026 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2690 3611 4208 4995 5263 1239 Size: 393 737 321 921 597 787 268 1524 Fragments arranged by size: 1524 921 787 737 597 393 321 268 EcoNI CCTnn'n_nnAGG Cuts at: 2585 2585 Size: 5548 EcoO109I rG'GnC_Cy Cuts at: 2238 2239 2701 2238 Size: 1 462 5085 Fragments arranged by size: 5085 462 1 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2146 2611 2146 Size: 465 5083 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 4695 1678 Size: 3017 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373 Size: 32 135 32 2617 32 42 32 2626 Fragments arranged by size: 2626 2617 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 2356 748 Size: 1608 3940 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2499 4662 4846 1305 Size: 1194 2163 184 2007 Fragments arranged by size: 2163 2007 1194 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 10 Size: 54 1425 343 357 3369 Fragments arranged by size: 3369 1425 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3506 3865 4024 518 Size: 648 2340 359 159 2042 Fragments arranged by size: 2340 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2256 2338 2347 2417 2452 2964 3641 4789 Size: 650 82 9 70 35 512 677 1148 Cuts at: 4789 5034 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 650 512 245 82 70 35 9 NcoI C'CATG_G Cuts at: 513 1065 2208 513 Size: 552 1143 3853 Fragments arranged by size: 3853 1143 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PasI CC'CwG_GG Cuts at: 1348 2314 1348 Size: 966 4582 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2762 2794 3617 3649 5130 5162 2762 Size: 32 823 32 1481 32 3148 Fragments arranged by size: 3148 1481 823 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PspOMI G'GGCC_C Cuts at: 2238 2340 2238 Size: 102 5446 PspXI vC'TCGA_Gb Cuts at: 2768 2768 Size: 5548 PstI C_TGCA'G Cuts at: 830 2169 2668 2900 830 Size: 1339 499 232 3478 Fragments arranged by size: 3478 1339 499 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2347 2452 2347 Size: 105 5443 SacI G_AGCT'C Cuts at: 2253 2775 2253 Size: 522 5026 SacII CC_GC'GG Cuts at: 2418 2418 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 2042 3796 5508 2042 Size: 1754 1712 2082 Fragments arranged by size: 2082 1754 1712 SfcI C'TryA_G Cuts at: 826 1045 2165 2664 2896 4031 4712 4903 826 Size: 219 1120 499 232 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 681 499 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2875 1872 Size: 1003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 2768 3652 4553 4815 820 Size: 197 926 300 525 884 901 262 1553 Fragments arranged by size: 1553 926 901 884 525 300 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2208 2987 513 Size: 552 1143 779 3074 Fragments arranged by size: 3074 1143 779 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3575 3592 3745 3930 4375 325 Size: 1255 1995 17 153 185 445 1498 Fragments arranged by size: 1995 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 3794 5506 Size: 274 80 33 51 1443 63 1754 1712 Cuts at: 5506 96 Size: 138 Fragments arranged by size: 1754 1712 1443 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 2819 1316 Size: 1503 4045 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 2768 1943 Size: 825 4723 XmaI C'CCGG_G Cuts at: 1870 2873 1870 Size: 1003 4545 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2368 3356 276 Size: 53 83 186 936 834 988 2468 Fragments arranged by size: 2468 988 936 834 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI