################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiHKAI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI DdeI DpnII DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NdeII NheI NlaIII NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AatII 265 GACGTC GACGT^C Asp700I 333 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 192 CCCGGG C^[CT]CG[GA]G BbsI 337 GTCTTC GAAGAC(2/6) BglI 28 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 91 GGGCAC G[AGT]GC[ACT]^C BpuAI 337 GTCTTC GAAGAC(2/6) BsgI 226 CTGCAC GTGCAG(16/14) BsiWI 152 CGTACG C^GTACG BsoBI 192 CCCGGG C^[CT]CG[GA]G Bsp1286I 91 GGGCAC G[AGT]GC[ACT]^C BsrBI 292 GAGCGG CCGCTC(-3/-3) BsrFI 202 GCCGGC [GA]^CCGG[CT] BssHII 351 GCGCGC G^CGCGC Cfr10I 202 GCCGGC [GA]^CCGG[CT] CpoI 7 CGGTCCG CG^G[AT]CCG HgaI 257 GCGTC GACGC(5/10) MboII 338 TCTTC GAAGA(8/7) NaeI 202 GCCGGC GCC^GGC NgoMIV 202 GCCGGC G^CCGGC NotI 22 GCGGCCGC GC^GGCCGC PstI 280 CTGCAG CTGCA^G RsrII 7 CGGTCCG CG^G[AT]CCG SmaI 192 CCCGGG CCC^GGG XmaCI 192 CCCGGG C^CCGGG XmaI 192 CCCGGG C^CCGGG XmnI 333 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 75 GTAC GT^AC 153 GTAC AluI 97 AGCT AG^CT 218 AGCT BanI 54 GGCGCC G^G[CT][GA]CC 205 GGCGCC BbeI 54 GGCGCC GGCGC^C 205 GGCGCC BsiEI 23 CGGCCG CG[GA][CT]^CG 306 CGGCCG BspMI 278 ACCTGC ACCTGC(4/8) 143 GCAGGT BsrI 18 ACTGG ACTGG(1/-1) 321 CCAGT DraII 145 AGGTCCC [GA]G^G[ACGT]CC[CT] 313 GGGACCT DsaI 119 CCGCGG C^C[GA][CT]GG 309 CCGCGG EaeI 23 CGGCCG [CT]^GGCC[GA] 306 CGGCCG EagI 23 CGGCCG C^GGCCG 306 CGGCCG EclXI 23 CGGCCG C^GGCCG 306 CGGCCG Eco52I 23 CGGCCG C^GGCCG 306 CGGCCG EcoO109I 145 AGGTCCC [GA]G^G[ACGT]CC[CT] 313 GGGACCT KasI 54 GGCGCC G^GCGCC 205 GGCGCC KspI 119 CCGCGG CCGC^GG 309 CCGCGG MaeII 266 ACGT A^CGT 335 ACGT NarI 54 GGCGCC GG^CGCC 205 GGCGCC PpuMI 145 AGGTCCC [GA]G^G[AT]CC[CT] 313 GGGACCT RsaI 75 GTAC GT^AC 153 GTAC SacII 119 CCGCGG CCGC^GG 309 CCGCGG SfcI 174 CTACAG C^T[GA][CT]AG 280 CTGCAG SfoI 54 GGCGCC GGC^GCC 205 GGCGCC TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^ 322 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AcyI AvaII BbvI BcnI BsaHI BsaJI BsiYI BslI BsmFI BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI