(Circular) (Six-base) MAPSORT of: pFN21AE3020.seq Check: 4389 from: 1 to: 5080 pFN21AE3020 5080 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2274 2890 278 Size: 53 83 186 936 738 616 2468 Fragments arranged by size: 2468 936 738 616 186 83 53 AccI GT'mk_AC Cuts at: 1937 2423 1937 Size: 486 4594 Acc65I G'GTAC_C Cuts at: 2401 2401 Size: 5080 AclI AA'CG_TT Cuts at: 1563 3207 3580 1563 Size: 1644 373 3063 Fragments arranged by size: 3063 1644 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3143 4527 4795 1632 Size: 1511 1384 268 1917 Fragments arranged by size: 1917 1511 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4883 AflIII A'CryG_T Cuts at: 1203 3979 4956 1203 Size: 2776 977 1327 Fragments arranged by size: 2776 1327 977 AgeI A'CCGG_T Cuts at: 3911 3911 Size: 5080 AhdI GACnn_n'nnGTC Cuts at: 3809 3809 Size: 5080 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5080 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2383 1184 Size: 1199 3881 AlwNI CAG_nnn'CTG Cuts at: 2823 4395 2823 Size: 1572 3508 ApaLI G'TGCA_C Cuts at: 3137 4293 3137 Size: 1156 3924 ApoI r'AATT_y Cuts at: 1493 2389 2615 2767 4876 1493 Size: 896 226 152 2109 1697 Fragments arranged by size: 2109 1697 896 226 152 AseI AT'TA_AT Cuts at: 160 1032 3634 160 Size: 872 2602 1606 Fragments arranged by size: 2602 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5080 AvaI C'yCGr_G Cuts at: 1870 1943 2197 2405 1870 Size: 73 254 208 4545 Fragments arranged by size: 4545 254 208 73 BamHI G'GATC_C Cuts at: 2410 2777 2410 Size: 367 4713 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2210 2401 3856 618 Size: 325 217 627 272 151 191 1455 1842 Fragments arranged by size: 1842 1455 627 325 272 217 191 151 BanII G_rGCy'C Cuts at: 1391 2399 1391 Size: 1008 4072 BbeI G_GCGC'C Cuts at: 2063 2214 2063 Size: 151 4929 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2335 928 Size: 971 436 3673 Fragments arranged by size: 3673 971 436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3271 3305 1903 Size: 34 1334 34 3678 Fragments arranged by size: 3678 1334 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2972 4182 2972 Size: 1210 3870 BclI T'GATC_A Cuts at: 1548 1548 Size: 5080 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 3691 136 Size: 107 122 71 631 605 367 1652 1525 Fragments arranged by size: 1652 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 5075 5075 Size: 5080 BlpI GC'TnA_GC Cuts at: 2497 2497 Size: 5080 Bme1580I G_kGCm'C Cuts at: 1790 2100 3141 4297 1790 Size: 310 1041 1156 2573 Fragments arranged by size: 2573 1156 1041 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5080 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2811 3769 442 Size: 416 53 83 399 24 1394 958 1753 Fragments arranged by size: 1753 1394 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5080 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3740 1239 Size: 2501 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3205 4070 4368 3205 Size: 865 298 3917 Fragments arranged by size: 3917 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3743 882 Size: 617 2244 2219 Fragments arranged by size: 2244 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2871 493 Size: 711 1667 2702 Fragments arranged by size: 2702 1667 711 BsaBI GATnn'nnATC Cuts at: 5074 5074 Size: 5080 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2211 2271 2887 Size: 53 83 186 936 527 151 60 616 Cuts at: 2887 3269 3904 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 616 527 382 186 151 83 60 53 BsaWI w'CCGG_w Cuts at: 3513 3911 4185 4332 4743 3513 Size: 398 274 147 411 3850 Fragments arranged by size: 3850 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4855 Fragments arranged by size: 4855 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5080 BseYI C'CCAG_C Cuts at: 2172 4283 4779 2172 Size: 2111 496 2473 Fragments arranged by size: 2473 2111 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 1163 Size: 573 480 4027 Fragments arranged by size: 4027 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 2314 2530 3291 4319 1540 Size: 275 186 30 283 216 761 1028 2301 Fragments arranged by size: 2301 1028 761 283 275 216 186 30 BsiHKAI G_wGCw'C Cuts at: 3141 3226 4297 3141 Size: 85 1071 3924 Fragments arranged by size: 3924 1071 85 BsiWI C'GTAC_G Cuts at: 2157 2157 Size: 5080 BsmI GAATG_Cn' Cuts at: 2591 2684 2591 Size: 93 4987 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2399 3141 3226 4297 1391 Size: 399 310 299 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 299 85 BspEI T'CCGG_A Cuts at: 4743 4743 Size: 5080 BspHI T'CATG_A Cuts at: 2969 4699 2969 Size: 1730 3350 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2292 2435 844 Size: 1295 153 143 3489 Fragments arranged by size: 3489 1295 153 143 BsrBI CCG'CTC Cuts at: 1394 1823 2299 2967 1394 Size: 429 476 668 3507 Fragments arranged by size: 3507 668 476 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3575 3749 65 Size: 1183 2327 174 1396 Fragments arranged by size: 2327 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2207 3724 3911 1700 Size: 110 397 1517 187 2869 Fragments arranged by size: 2869 1517 397 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5080 BssHII G'CGCG_C Cuts at: 1925 2356 1925 Size: 431 4649 BssSI C'ACGA_G Cuts at: 3140 4152 3140 Size: 1012 4068 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5080 BstBI TT'CG_AA Cuts at: 3884 3884 Size: 5080 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5080 BstYI r'GATC_y Cuts at: 1972 2410 2777 3162 3179 4631 4717 4729 5075 Size: 438 367 385 17 1452 86 12 346 Cuts at: 5075 1972 Size: 1977 Fragments arranged by size: 1977 1452 438 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2124 2314 513 Size: 552 939 120 190 3279 Fragments arranged by size: 3279 939 552 190 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4341 Fragments arranged by size: 4341 683 56 BtsI GCAGTG_nn' Cuts at: 2596 3390 3410 2596 Size: 794 20 4266 Fragments arranged by size: 4266 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5080 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5045 DraI TTT'AAA Cuts at: 2384 2384 Size: 5080 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4087 809 Size: 507 2771 1802 Fragments arranged by size: 2771 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2311 2527 Size: 54 1425 50 275 18 198 283 216 Cuts at: 2527 3416 5045 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 283 275 216 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2028 2311 2527 1537 Size: 275 216 283 216 4090 Fragments arranged by size: 4090 283 275 216 216 EarI CTCTTCn'nnn_ Cuts at: 1353 3010 1353 Size: 1657 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2204 3667 4041 4187 387 Size: 1064 138 615 1463 374 146 1280 Fragments arranged by size: 1463 1280 1064 615 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3143 3740 4527 4795 1239 Size: 393 1511 597 787 268 1524 Fragments arranged by size: 1524 1511 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2151 2319 2151 Size: 168 4912 EcoRI G'AATT_C Cuts at: 2389 2767 2389 Size: 378 4702 FspI TGC'GCA Cuts at: 3586 3586 Size: 5080 HaeII r_GCGC'y Cuts at: 1678 2045 2063 2170 2214 4227 1678 Size: 367 18 107 44 2013 2531 Fragments arranged by size: 2531 2013 367 107 44 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3721 3753 3795 3827 1373 Size: 32 135 32 2149 32 42 32 2626 Fragments arranged by size: 2626 2149 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2424 2670 669 Size: 988 281 486 246 3079 Fragments arranged by size: 3079 988 486 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5080 HpaI GTT'AAC Cuts at: 2670 2670 Size: 5080 KasI G'GCGC_C Cuts at: 2059 2210 2059 Size: 151 4929 KpnI G_GTAC'C Cuts at: 2405 2405 Size: 5080 MfeI C'AATT_G Cuts at: 2679 2679 Size: 5080 MluI A'CGCG_T Cuts at: 4956 4956 Size: 5080 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4194 4378 1305 Size: 2889 184 2007 Fragments arranged by size: 2889 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3258 Fragments arranged by size: 3258 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3038 3397 3556 518 Size: 648 1872 359 159 2042 Fragments arranged by size: 2042 1872 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2090 2126 2316 2496 3173 4321 4566 1606 Size: 484 36 190 180 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 484 245 190 180 36 NaeI GCC'GGC Cuts at: 2209 2209 Size: 5080 NarI GG'CG_CC Cuts at: 2060 2211 2060 Size: 151 4929 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3589 Fragments arranged by size: 3589 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5080 NgoMIV G'CCGG_C Cuts at: 2207 2207 Size: 5080 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5080 NotI GC'GGCC_GC Cuts at: 2028 2527 2028 Size: 499 4581 NspI r_CATG'y Cuts at: 1132 1229 2438 3983 1132 Size: 97 1209 1545 2229 Fragments arranged by size: 2229 1545 1209 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5080 PciI A'CATG_T Cuts at: 3979 3979 Size: 5080 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5080 PfoI T'CCnGG_A Cuts at: 2265 2265 Size: 5080 PmeI GTTT'AAAC Cuts at: 2384 2384 Size: 5080 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3149 3181 4662 4694 3149 Size: 32 1481 32 3535 Fragments arranged by size: 3535 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2151 2319 2151 Size: 168 4912 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5080 PsiI TTA'TAA Cuts at: 2650 2650 Size: 5080 PstI C_TGCA'G Cuts at: 830 2289 2432 830 Size: 1459 143 3478 Fragments arranged by size: 3478 1459 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5080 RsrII CG'GwC_CG Cuts at: 2013 2013 Size: 5080 SacII CC_GC'GG Cuts at: 2127 2317 2127 Size: 190 4890 SalI G'TCGA_C Cuts at: 1936 2422 1936 Size: 486 4594 SbfI CC_TGCA'GG Cuts at: 2432 2432 Size: 5080 ScaI AGT'ACT Cuts at: 3328 5040 3328 Size: 1712 3368 SfcI C'TryA_G Cuts at: 826 1045 2179 2285 2428 3563 4244 4435 826 Size: 219 1134 106 143 1135 681 191 1471 Fragments arranged by size: 1471 1135 1134 681 219 191 143 106 SfoI GGC'GCC Cuts at: 2061 2212 2061 Size: 151 4929 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5080 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5080 SmaI CCC'GGG Cuts at: 1872 2199 2407 1872 Size: 327 208 4545 Fragments arranged by size: 4545 327 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 3184 4085 4347 820 Size: 197 926 1241 901 262 1553 Fragments arranged by size: 1553 1241 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2871 493 Size: 2378 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5080 SphI G_CATG'C Cuts at: 1132 2438 1132 Size: 1306 3774 SspI AAT'ATT Cuts at: 5 52 3004 5 Size: 47 2952 2081 Fragments arranged by size: 2952 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5080 StyI C'CwwG_G Cuts at: 513 1065 2004 2519 513 Size: 552 939 515 3074 Fragments arranged by size: 3074 939 552 515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 3124 3277 3462 3907 325 Size: 1255 1527 17 153 185 445 1498 Fragments arranged by size: 1527 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3326 5038 96 Size: 274 80 33 51 1443 1349 1712 138 Fragments arranged by size: 1712 1443 1349 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5040 5072 5040 Size: 32 5048 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5080 XbaI T'CTAG_A Cuts at: 2416 2416 Size: 5080 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5080 XmaI C'CCGG_G Cuts at: 1870 2197 2405 1870 Size: 327 208 4545 Fragments arranged by size: 4545 327 208 XmnI GAAnn'nnTTC Cuts at: 2342 3209 2342 Size: 867 4213 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2272 2888 276 Size: 53 83 186 936 738 616 2468 Fragments arranged by size: 2468 936 738 616 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI