################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 115 CGCG CG^CG AflIII 114 ACGCGT A^C[GA][CT]GT AlwI 60 GATCC GGATC(4/5) AocI 578 CCTCAGG CC^T[ACGT]AGG AvaII 377 GGACC G^G[AT]CC BalI 335 TGGCCA TGG^CCA BanI 417 GGTGCC G^G[CT][GA]CC BbsI 348 GAAGAC GAAGAC(2/6) BclI 155 TGATCA T^GATCA BpuAI 348 GAAGAC GAAGAC(2/6) BsaWI 206 ACCGGA [AT]^CCGG[AT] BsmFI 376 GGGAC GGGAC(10/14) BspMI 14 ACCTGC ACCTGC(4/8) BsrDI 684 GCAATG GCAATG(2/0) BstUI 115 CGCG CG^CG BstYI 59 AGATCC [GA]^GATC[CT] Bsu36I 578 CCTCAGG CC^T[ACGT]AGG CfoI 223 GCGC GCG^C DraIII 544 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 113 GACGCGTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 578 CCTCAGG CC^T[ACGT]AGG EcoNI 380 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 229 CCCGC CCCGC(4/6) FbaI 155 TGATCA T^GATCA HaeII 222 GGCGCT [GA]GCGC^[CT] HhaI 223 GCGC GCG^C HinP1I 223 GCGC G^CGC MaeII 248 ACGT A^CGT MboII 348 GAAGA GAAGA(8/7) MflI 59 AGATCC [GA]^GATC[CT] MluI 114 ACGCGT A^CGCGT MluNI 335 TGGCCA TGG^CCA MscI 335 TGGCCA TGG^CCA MseI 11 TTAA T^TAA MspA1I 421 CCGCTG C[AC]G^C[GT]G MvnI 115 CGCG CG^CG PpuMI 376 GGGACCT [GA]G^G[AT]CC[CT] SexAI 413 ACCTGGT A^CC[AT]GGT SfaNI 284 GCATC GCATC(5/9) SfcI 180 CTACAG C^T[GA][CT]AG SfiI 252 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 50 CCCGGG CCC^GGG StuI 575 AGGCCT AGG^CCT Tru9I 11 TTAA T^TAA Tsp509I 357 AATT ^AATT XcmI 404 CCATCCTGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 59 AGATCC [GA]^GATC[CT] XmaCI 50 CCCGGG C^CCGGG XmaI 50 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 141 GTAC GT^AC 540 GTAC AlwNI 189 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 676 CAGATTCTG AvaI 50 CCCGGG C^[CT]CG[GA]G 73 CTCGGG BglI 253 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 588 GCCTGCCGGGC BsgI 195 CTGCAC GTGCAG(16/14) 365 CTGCAC BsmAI 111 GAGAC GTCTC(1/5) 245 GAGAC BsmBI 111 GAGACG CGTCTC(1/5) 245 GAGACG BsoBI 50 CCCGGG C^[CT]CG[GA]G 73 CTCGGG BsrI 369 ACTGG ACTGG(1/-1) 619 CCAGT BstF5I 405 CATCC GGATG(2/0) 372 GGATG DpnII 60 GATC ^GATC 156 GATC DraII 260 AGGCCCT [GA]G^G[ACGT]CC[CT] 376 GGGACCT EaeI 335 TGGCCA [CT]^GGCC[GA] 425 TGGCCG EcoO109I 260 AGGCCCT [GA]G^G[ACGT]CC[CT] 376 GGGACCT EcoT14I 215 CCATGG C^C[AT][AT]GG 332 CCATGG FokI 405 CATCC GGATG(9/13) 372 GGATG HgaI 113 GACGC GACGC(5/10) 116 GCGTC HinfI 69 GATTC G^A[ACGT]TC 678 GATTC MboI 60 GATC ^GATC 156 GATC NcoI 215 CCATGG C^CATGG 332 CCATGG NdeII 60 GATC ^GATC 156 GATC RsaI 141 GTAC GT^AC 540 GTAC Sau3AI 60 GATC ^GATC 156 GATC StyI 215 CCATGG C^C[AT][AT]GG 332 CCATGG TaqI 287 TCGA T^CGA 515 TCGA TfiI 69 GATTC G^A[AT]TC 678 GATTC TthHB8I 287 TCGA T^CGA 515 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BcnI BsaJI BsiYI BslI BssKI BstAPI BstNI Cac8I Cfr13I DdeI DsaI EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI TspRI