Restriction map for pFN21AB6977
 (Circular) (Six-base) MAPSORT of: pFN21AB6977.seq  Check: 2627  from: 1  to: 5356



pFN21AB6977  5356 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:55  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2027    3166     278 
   Size:         53      83     186     936     491    1139    2468

  Fragments arranged by size: 

                2468    1139     936     491     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2699    1937 
   Size:        762    4594
 
Acc65I G'GTAC_C

Cuts at:   2677    2677 
   Size:       5356
 
AclI AA'CG_TT

Cuts at:   1563    3483    3856    1563 
   Size:       1920     373    3063

  Fragments arranged by size: 

                3063    1920     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2563    3419    4803    5071    1632 
   Size:        931     856    1384     268    1917

  Fragments arranged by size: 

                1917    1384     931     856     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5159
 
AflIII A'CryG_T

Cuts at:   1203    4255    5232    1203 
   Size:       3052     977    1327

  Fragments arranged by size: 

                3052    1327     977
 
AgeI A'CCGG_T

Cuts at:   4187    4187 
   Size:       5356
 
AhdI GACnn_n'nnGTC

Cuts at:   4085    4085 
   Size:       5356
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5356
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2336    1184 
   Size:       1152    4204
 
AlwNI CAG_nnn'CTG

Cuts at:   3099    4671    3099 
   Size:       1572    3784
 
ApaLI G'TGCA_C

Cuts at:   3413    4569    3413 
   Size:       1156    4200
 
ApoI r'AATT_y

Cuts at:   1493    2665    2891    3043    5152    1493 
   Size:       1172     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1172     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3910     160 
   Size:        872    2878    1606

  Fragments arranged by size: 

                2878    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5356
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2681    1870 
   Size:         73     738    4545

  Fragments arranged by size: 

                4545     738      73
 
BamHI G'GATC_C

Cuts at:   2686    3053    2686 
   Size:        367    4989
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2393    2677    4132     618 
   Size:        325     217     627     606     284    1455    1842

  Fragments arranged by size: 

                1842    1455     627     606     325     284     217
 
BanII G_rGCy'C

Cuts at:   1391    2349    2675    1391 
   Size:        958     326    4072

  Fragments arranged by size: 

                4072     958     326
 
BbeI G_GCGC'C

Cuts at:   2397    2397 
   Size:       5356
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2362     928 
   Size:        971     463    3922

  Fragments arranged by size: 

                3922     971     463
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3547    3581    1903 
   Size:         34    1610      34    3678

  Fragments arranged by size: 

                3678    1610      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3248    4458    3248 
   Size:       1210    4146
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5356
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3967     136 
   Size:        107     122      71     631     605    2295    1525

  Fragments arranged by size: 

                2295    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2493    5351    2493 
   Size:       2858    2498
 
BlpI GC'TnA_GC

Cuts at:   2773    2773 
   Size:       5356
 
Bme1580I G_kGCm'C

Cuts at:   1790    3417    4573    1790 
   Size:       1627    1156    2573

  Fragments arranged by size: 

                2573    1627    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5356
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2496    3087    4045 
   Size:        416      53      83     399      24    1079     591     958

Cuts at:   4045     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1079     958     591     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5356
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2363    4016    1239 
   Size:       1124    1653    2579

  Fragments arranged by size: 

                2579    1653    1124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2537    3481    4346    4644    2537 
   Size:        944     865     298    3249

  Fragments arranged by size: 

                3249     944     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4019     882 
   Size:        617    2520    2219

  Fragments arranged by size: 

                2520    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3147     493 
   Size:        711    1943    2702

  Fragments arranged by size: 

                2702    1943     711
 
BsaBI GATnn'nnATC

Cuts at:   5350    5350 
   Size:       5356
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2024    2394    3163    3545 
   Size:         53      83     186     936     491     370     769     382

Cuts at:   3545    4180     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     769     635     491     382     370     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   3789    4187    4461    4608    5019    3789 
   Size:        398     274     147     411    4126

  Fragments arranged by size: 

                4126     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5131

  Fragments arranged by size: 

                5131     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5356
 
BseYI C'CCAG_C

Cuts at:   4559    5055    4559 
   Size:        496    4860
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2126    2372    2642    1163 
   Size:        573     390     246     270    3877

  Fragments arranged by size: 

                3877     573     390     270     246
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2806    3567    4595    1540 
   Size:        275     186     805     761    1028    2301

  Fragments arranged by size: 

                2301    1028     805     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3417    3502    4573    3417 
   Size:         85    1071    4200

  Fragments arranged by size: 

                4200    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2867    2960    2867 
   Size:         93    5263
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2349    2675    3417    3502    4573    1391 
   Size:        399     559     326     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     559     399     326      85
 
BspEI T'CCGG_A

Cuts at:   5019    5019 
   Size:       5356
 
BspHI T'CATG_A

Cuts at:   3245    4975    3245 
   Size:       1730    3626
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2711     844 
   Size:       1867    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2631    3243    1394 
   Size:        429     808     612    3507

  Fragments arranged by size: 

                3507     808     612     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3851    4025      65 
   Size:       1183    2603     174    1396

  Fragments arranged by size: 

                2603    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4000    4187    1700 
   Size:        110    2190     187    2869

  Fragments arranged by size: 

                2869    2190     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5356
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5356
 
BssSI C'ACGA_G

Cuts at:   3416    4428    3416 
   Size:       1012    4344
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5356
 
BstBI TT'CG_AA

Cuts at:   4160    4160 
   Size:       5356
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5356
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2383    2383 
   Size:       5356
 
BstYI r'GATC_y

Cuts at:   1972    2493    2686    3053    3438    3455    4907    4993    5005 
   Size:        521     193     367     385      17    1452      86      12

Cuts at:   5005    5351    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     521     385     367     346     193      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2360    2360 
   Size:       5356
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3865

  Fragments arranged by size: 

                3865     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4617

  Fragments arranged by size: 

                4617     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2083    2630    2872    3666    3686    2083 
   Size:        547     242     794      20    3753

  Fragments arranged by size: 

                3753     794     547     242      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5356
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5321
 
DraI TTT'AAA

Cuts at:   2660    2660 
   Size:       5356
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2408    4363     809 
   Size:        507    1092    1955    1802

  Fragments arranged by size: 

                1955    1802    1092     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2803    3692    5321 
   Size:         54    1425      50     275      18     973     889    1629

Cuts at:   5321       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     973     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2803    1537 
   Size:        275     991    4090

  Fragments arranged by size: 

                4090     991     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3286    1353 
   Size:       1933    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3943    4317    4463     387 
   Size:       1064     138    2354     374     146    1280

  Fragments arranged by size: 

                2354    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2363    2563    3419    4016    4803    5071    1239 
   Size:        393     731     200     856     597     787     268    1524

  Fragments arranged by size: 

                1524     856     787     731     597     393     268     200
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2101    2269    2423    2101 
   Size:        168     154    5034

  Fragments arranged by size: 

                5034     168     154
 
EcoO109I rG'GnC_Cy

Cuts at:   2428    2428 
   Size:       5356
 
EcoRI G'AATT_C

Cuts at:   2665    3043    2665 
   Size:        378    4978
 
FspI TGC'GCA

Cuts at:   2615    3862    2615 
   Size:       1247    4109
 
HaeII r_GCGC'y

Cuts at:   1678    2397    4503    1678 
   Size:        719    2106    2531

  Fragments arranged by size: 

                2531    2106     719
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2513    2545    3997    4029    4071 
   Size:         32     135      32     941      32    1452      32      42

Cuts at:   4071    4103    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1452     941     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2700    2946     669 
   Size:        988     281     762     246    3079

  Fragments arranged by size: 

                3079     988     762     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5356
 
HpaI GTT'AAC

Cuts at:   2946    2946 
   Size:       5356
 
KasI G'GCGC_C

Cuts at:   2393    2393 
   Size:       5356
 
KpnI G_GTAC'C

Cuts at:   2681    2681 
   Size:       5356
 
MfeI C'AATT_G

Cuts at:   2955    2955 
   Size:       5356
 
MluI A'CGCG_T

Cuts at:   5232    5232 
   Size:       5356
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2282    2292    2319    4470    4654    1305 
   Size:        977      10      27    2151     184    2007

  Fragments arranged by size: 

                2151    2007     977     184      27      10
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3534

  Fragments arranged by size: 

                3534    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2538    3314    3673    3832     518 
   Size:        648    1372     776     359     159    2042

  Fragments arranged by size: 

                2042    1372     776     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2149    2646    2772    3449    4597    4842    1606 
   Size:        543     497     126     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     543     497     245     126
 
NarI GG'CG_CC

Cuts at:   2394    2394 
   Size:       5356
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3865

  Fragments arranged by size: 

                3865     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5356
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5356
 
NotI GC'GGCC_GC

Cuts at:   2803    2803 
   Size:       5356
 
NspI r_CATG'y

Cuts at:   1132    1229    2714    4259    1132 
   Size:         97    1485    1545    2229

  Fragments arranged by size: 

                2229    1545    1485      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5356
 
PciI A'CATG_T

Cuts at:   4255    4255 
   Size:       5356
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5356
 
PfoI T'CCnGG_A

Cuts at:   2018    2018 
   Size:       5356
 
PmeI GTTT'AAAC

Cuts at:   2660    2660 
   Size:       5356
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3425    3457    4938    4970    3425 
   Size:         32    1481      32    3811

  Fragments arranged by size: 

                3811    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2428    2428 
   Size:       5356
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5356
 
PsiI TTA'TAA

Cuts at:   2926    2926 
   Size:       5356
 
PstI C_TGCA'G

Cuts at:    830    2220    2708     830 
   Size:       1390     488    3478

  Fragments arranged by size: 

                3478    1390     488
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5356
 
PvuII CAG'CTG

Cuts at:   2149    2646    2149 
   Size:        497    4859
 
SalI G'TCGA_C

Cuts at:   1936    2698    1936 
   Size:        762    4594
 
SbfI CC_TGCA'GG

Cuts at:   2708    2708 
   Size:       5356
 
ScaI AGT'ACT

Cuts at:   3604    5316    3604 
   Size:       1712    3644
 
SfcI C'TryA_G

Cuts at:    826    1045    2010    2216    2509    2704    3839    4520    4711 
   Size:        219     965     206     293     195    1135     681     191

Cuts at:   4711     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135     965     681     293     219     206     195
                 191
 
SfoI GGC'GCC

Cuts at:   2395    2395 
   Size:       5356
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5356
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5356
 
SmaI CCC'GGG

Cuts at:   1872    2683    1872 
   Size:        811    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2552    3460    4361    4623     820 
   Size:        197     926     609     908     901     262    1553

  Fragments arranged by size: 

                1553     926     908     901     609     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3147     493 
   Size:       2654    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5356
 
SphI G_CATG'C

Cuts at:   1132    2714    1132 
   Size:       1582    3774
 
SspI AAT'ATT

Cuts at:      5      52    3280       5 
   Size:         47    3228    2081

  Fragments arranged by size: 

                3228    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5356
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2795     513 
   Size:        552     939     791    3074

  Fragments arranged by size: 

                3074     939     791     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3383    3400    3553    3738    4183     325 
   Size:       1255    1803      17     153     185     445    1498

  Fragments arranged by size: 

                1803    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3602    5314      96 
   Size:        274      80      33      51    1443    1625    1712     138

  Fragments arranged by size: 

                1712    1625    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5316    5348    5316 
   Size:         32    5324
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2597    1316 
   Size:       1281    4075
 
XbaI T'CTAG_A

Cuts at:   2692    2692 
   Size:       5356
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5356
 
XmaI C'CCGG_G

Cuts at:   1870    2681    1870 
   Size:        811    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3485    3485 
   Size:       5356
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2025    3164     276 
   Size:         53      83     186     936     491    1139    2468

  Fragments arranged by size: 

                2468    1139     936     491     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI
    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI
     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SrfI     SwaI     XcmI
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