(Circular) (Six-base) MAPSORT of: pFN21AE4307.seq Check: 2469 from: 1 to: 5641 pFN21AE4307 5641 bp With 182 enzymes: SgfI * March 28, 2012 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2358 2358 Size: 5641 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3704 5088 5356 1632 Size: 870 1202 1384 268 1917 Fragments arranged by size: 1917 1384 1202 870 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 2155 4370 2155 Size: 2215 3426 AleI CACnn'nnGTG Cuts at: 1166 2071 1166 Size: 905 4736 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2551 2752 2770 2830 3384 4956 2551 Size: 201 18 60 554 1572 3236 Fragments arranged by size: 3236 1572 554 201 60 18 ApaI G_GGCC'C Cuts at: 2863 2887 2906 2863 Size: 24 19 5598 Fragments arranged by size: 5598 24 19 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2863 2966 1870 Size: 73 920 103 4545 Fragments arranged by size: 4545 920 103 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2116 2962 4417 618 Size: 325 217 627 329 846 1455 1842 Fragments arranged by size: 1842 1455 846 627 329 325 217 BanII G_rGCy'C Cuts at: 1391 2381 2672 2727 2863 2887 2906 2960 1391 Size: 990 291 55 136 24 19 54 4072 Fragments arranged by size: 4072 990 291 136 55 54 24 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2490 928 Size: 971 591 4079 Fragments arranged by size: 4079 971 591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3832 3866 1903 Size: 34 50 34 1811 34 3678 Fragments arranged by size: 3678 1811 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 2268 2863 2887 2906 3702 4858 1790 Size: 478 595 24 19 796 1156 2573 Fragments arranged by size: 2573 1156 796 595 478 24 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2213 3372 4330 Size: 416 53 83 399 24 796 1159 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1159 958 796 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2328 4301 1239 Size: 1067 22 1973 2579 Fragments arranged by size: 2579 1973 1067 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2579 1178 Size: 30 165 30 1146 30 4240 Fragments arranged by size: 4240 1146 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2325 2651 1186 Size: 1139 326 4176 Fragments arranged by size: 4176 1139 326 BseYI C'CCAG_C Cuts at: 2830 2899 4844 5340 2830 Size: 69 1945 496 3131 Fragments arranged by size: 3131 1945 496 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2079 2336 1163 Size: 573 343 257 4468 Fragments arranged by size: 4468 573 343 257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2873 3091 3852 4880 1540 Size: 275 186 872 218 761 1028 2301 Fragments arranged by size: 2301 1028 872 761 275 218 186 BsiHKAI G_wGCw'C Cuts at: 2388 2406 2594 3702 3787 4858 2388 Size: 18 188 1108 85 1071 3171 Fragments arranged by size: 3171 1108 1071 188 85 18 BsmI GAATG_Cn' Cuts at: 2756 3152 3245 2756 Size: 396 93 5152 Fragments arranged by size: 5152 396 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2381 2388 2406 2594 2672 2727 Size: 399 478 113 7 18 188 78 55 Cuts at: 2727 2863 2887 2906 2960 3702 3787 4858 1391 Size: 136 24 19 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 188 136 113 85 78 55 54 24 19 18 7 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 2631 2696 2996 844 Size: 1514 273 65 300 3489 Fragments arranged by size: 3489 1514 300 273 65 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2927 4285 4472 1700 Size: 110 1117 1358 187 2869 Fragments arranged by size: 2869 1358 1117 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstXI CCAn_nnnn'nTGG Cuts at: 2127 2127 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2748 2971 3338 3723 3740 5192 5278 5290 Size: 776 223 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 776 385 367 346 223 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2412 513 Size: 552 972 375 3742 Fragments arranged by size: 3742 972 552 375 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2706 487 Size: 683 56 1480 3422 Fragments arranged by size: 3422 1480 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2113 2148 529 Size: 35 1549 35 4022 Fragments arranged by size: 4022 1549 35 35 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2272 2272 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2052 2870 Size: 54 1425 50 275 18 215 7 818 Cuts at: 2870 2925 3088 3977 5606 8 Size: 55 163 889 1629 43 Fragments arranged by size: 1629 1425 889 818 275 215 163 55 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2870 3088 1537 Size: 275 1058 218 4090 Fragments arranged by size: 4090 1058 275 218 EarI CTCTTCn'nnn_ Cuts at: 1353 3571 1353 Size: 2218 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2328 2502 3704 4301 5088 5356 Size: 393 674 22 174 1202 597 787 268 Cuts at: 5356 1239 Size: 1524 Fragments arranged by size: 1524 1202 787 674 597 393 268 174 22 EcoO109I rG'GnC_Cy Cuts at: 2476 2817 2859 2860 2884 2903 2476 Size: 341 42 1 24 19 5214 Fragments arranged by size: 5214 341 42 24 19 1 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 2024 4788 1678 Size: 346 2764 2531 Fragments arranged by size: 2764 2531 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2549 2581 2733 Size: 32 135 32 569 32 376 32 152 Cuts at: 2733 2765 4282 4314 4356 4388 1373 Size: 32 1517 32 42 32 2626 Fragments arranged by size: 2626 1517 569 376 152 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 2385 2598 4755 4939 1305 Size: 819 261 213 2157 184 2007 Fragments arranged by size: 2157 2007 819 261 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2071 2417 2597 3599 3958 4117 Size: 648 843 62 346 180 1002 359 159 Cuts at: 4117 518 Size: 2042 Fragments arranged by size: 2042 1002 843 648 359 346 180 159 62 MspA1I CmG'CkG Cuts at: 1606 2039 2830 2875 3057 3734 4882 5127 1606 Size: 433 791 45 182 677 1148 245 2120 Fragments arranged by size: 2120 1148 791 677 433 245 182 45 NaeI GCC'GGC Cuts at: 2929 2929 Size: 5641 NcoI C'CATG_G Cuts at: 513 1065 2412 513 Size: 552 1347 3742 Fragments arranged by size: 3742 1347 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NgoMIV G'CCGG_C Cuts at: 2927 2927 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 2880 1348 Size: 1532 4109 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 2551 1108 Size: 1443 4198 PfoI T'CCnGG_A Cuts at: 2718 2718 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 2476 Size: 5641 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2859 2883 2902 2859 Size: 24 19 5598 Fragments arranged by size: 5598 24 19 PstI C_TGCA'G Cuts at: 830 2826 2993 830 Size: 1996 167 3478 Fragments arranged by size: 3478 1996 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2830 2830 Size: 5641 SacII CC_GC'GG Cuts at: 2040 2040 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2822 2989 4124 4805 4996 826 Size: 219 1777 167 1135 681 191 1471 Fragments arranged by size: 1777 1471 1135 681 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2865 2968 1872 Size: 993 103 4545 Fragments arranged by size: 4545 993 103 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2181 2412 2631 3080 513 Size: 552 1116 231 219 449 3074 Fragments arranged by size: 3074 1116 552 449 231 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2428 2772 3887 Size: 274 80 33 51 1443 451 344 1115 Cuts at: 3887 5599 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1115 451 344 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2546 5601 5633 2514 Size: 32 3055 32 2522 Fragments arranged by size: 3055 2522 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2810 1316 Size: 1494 4147 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 2188 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2863 2966 1870 Size: 993 103 4545 Fragments arranged by size: 4545 993 103 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI