(Circular) (Six-base) MAPSORT of: pFN21AE1702.seq Check: 4996 from: 1 to: 5317 pFN21AE1702 5317 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2424 3127 278 Size: 53 83 186 936 888 703 2468 Fragments arranged by size: 2468 936 888 703 186 83 53 AccI GT'mk_AC Cuts at: 1937 2660 1937 Size: 723 4594 Acc65I G'GTAC_C Cuts at: 2638 2638 Size: 5317 AclI AA'CG_TT Cuts at: 1563 3444 3817 1563 Size: 1881 373 3063 Fragments arranged by size: 3063 1881 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3380 4764 5032 1632 Size: 1748 1384 268 1917 Fragments arranged by size: 1917 1748 1384 268 AfeI AGC'GCT Cuts at: 2184 2184 Size: 5317 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5120 AflIII A'CryG_T Cuts at: 1203 4216 5193 1203 Size: 3013 977 1327 Fragments arranged by size: 3013 1327 977 AgeI A'CCGG_T Cuts at: 4148 4148 Size: 5317 AhdI GACnn_n'nnGTC Cuts at: 4046 4046 Size: 5317 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5317 AlwNI CAG_nnn'CTG Cuts at: 2056 2299 2368 3060 4632 2056 Size: 243 69 692 1572 2741 Fragments arranged by size: 2741 1572 692 243 69 ApaI G_GGCC'C Cuts at: 2108 2108 Size: 5317 ApaLI G'TGCA_C Cuts at: 2070 3374 4530 2070 Size: 1304 1156 2857 Fragments arranged by size: 2857 1304 1156 ApoI r'AATT_y Cuts at: 1493 2626 2852 3004 5113 1493 Size: 1133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1133 226 152 AseI AT'TA_AT Cuts at: 160 1032 3871 160 Size: 872 2839 1606 Fragments arranged by size: 2839 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5317 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2384 2642 1870 Size: 73 282 159 258 4545 Fragments arranged by size: 4545 282 258 159 73 BamHI G'GATC_C Cuts at: 2647 3014 2647 Size: 367 4950 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2498 2638 4093 618 Size: 325 217 627 711 140 1455 1842 Fragments arranged by size: 1842 1455 711 627 325 217 140 BanII G_rGCy'C Cuts at: 1391 2030 2108 2272 2358 2636 1391 Size: 639 78 164 86 278 4072 Fragments arranged by size: 4072 639 278 164 86 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4346 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3508 3542 1903 Size: 34 1571 34 3678 Fragments arranged by size: 3678 1571 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3209 4419 3209 Size: 1210 4107 BclI T'GATC_A Cuts at: 1548 1548 Size: 5317 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2507 3928 136 Size: 107 122 71 631 605 835 1421 1525 Fragments arranged by size: 1525 1421 835 631 605 122 107 71 BglII A'GATC_T Cuts at: 5312 5312 Size: 5317 BlpI GC'TnA_GC Cuts at: 2734 2734 Size: 5317 Bme1580I G_kGCm'C Cuts at: 1790 2074 2108 3378 4534 1790 Size: 284 34 1270 1156 2573 Fragments arranged by size: 2573 1270 1156 284 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5317 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2474 3048 4006 Size: 416 53 83 399 24 1057 574 958 Cuts at: 4006 442 Size: 1753 Fragments arranged by size: 1753 1057 958 574 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2597 3977 1239 Size: 932 426 1380 2579 Fragments arranged by size: 2579 1380 932 426 Bpu10I CC'TnA_GC Cuts at: 2276 2276 Size: 5317 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2123 2338 3442 4307 4605 2123 Size: 215 1104 865 298 2835 Fragments arranged by size: 2835 1104 865 298 215 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3980 882 Size: 617 2481 2219 Fragments arranged by size: 2481 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3108 493 Size: 711 1904 2702 Fragments arranged by size: 2702 1904 711 BsaBI GATnn'nnATC Cuts at: 5311 5311 Size: 5317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2421 3124 3506 4141 Size: 53 83 186 936 888 703 382 635 Cuts at: 4141 275 Size: 1451 Fragments arranged by size: 1451 936 888 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3750 4148 4422 4569 4980 3750 Size: 398 274 147 411 4087 Fragments arranged by size: 4087 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2213 2570 2600 1178 Size: 30 165 30 780 30 357 30 3895 Fragments arranged by size: 3895 780 357 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 1186 Size: 1057 4260 BseYI C'CCAG_C Cuts at: 2288 2610 4520 5016 2288 Size: 322 1910 496 2589 Fragments arranged by size: 2589 1910 496 322 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 1163 Size: 573 369 4375 Fragments arranged by size: 4375 573 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2767 3528 4556 1540 Size: 275 186 766 761 1028 2301 Fragments arranged by size: 2301 1028 766 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 3378 3463 4534 2074 Size: 1304 85 1071 2857 Fragments arranged by size: 2857 1304 1071 85 BsmI GAATG_Cn' Cuts at: 2828 2921 2828 Size: 93 5224 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2074 2108 2272 2358 2636 3378 Size: 399 240 44 34 164 86 278 742 Cuts at: 3378 3463 4534 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 278 240 164 86 85 44 34 BspEI T'CCGG_A Cuts at: 4980 4980 Size: 5317 BspHI T'CATG_A Cuts at: 3206 4936 3206 Size: 1730 3587 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 2672 844 Size: 1179 649 3489 Fragments arranged by size: 3489 1179 649 BsrBI CCG'CTC Cuts at: 1394 1823 3204 1394 Size: 429 1381 3507 Fragments arranged by size: 3507 1381 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3812 3986 65 Size: 1183 2564 174 1396 Fragments arranged by size: 2564 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2506 3961 4148 1700 Size: 110 696 1455 187 2869 Fragments arranged by size: 2869 1455 696 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5317 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5317 BssSI C'ACGA_G Cuts at: 3377 4389 3377 Size: 1012 4305 BstAPI GCAn_nnn'nTGC Cuts at: 794 2056 794 Size: 1262 4055 BstBI TT'CG_AA Cuts at: 4121 4121 Size: 5317 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5317 BstXI CCAn_nnnn'nTGG Cuts at: 2101 2174 2101 Size: 73 5244 BstYI r'GATC_y Cuts at: 1972 2163 2376 2647 3014 3399 3416 4868 4954 Size: 191 213 271 367 385 17 1452 86 Cuts at: 4954 4966 5312 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 271 213 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3826 Fragments arranged by size: 3826 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2187 487 Size: 683 56 961 3617 Fragments arranged by size: 3617 961 683 56 BtsI GCAGTG_nn' Cuts at: 2408 2833 3627 3647 2408 Size: 425 794 20 4078 Fragments arranged by size: 4078 794 425 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5317 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5282 DraI TTT'AAA Cuts at: 2621 2621 Size: 5317 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4324 809 Size: 507 3008 1802 Fragments arranged by size: 3008 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 3653 5282 Size: 54 1425 50 275 18 934 889 1629 Cuts at: 5282 8 Size: 43 Fragments arranged by size: 1629 1425 934 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2764 1537 Size: 275 952 4090 Fragments arranged by size: 4090 952 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3247 1353 Size: 1894 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3904 4278 4424 387 Size: 1064 138 2315 374 146 1280 Fragments arranged by size: 2315 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2597 3380 3977 4764 5032 1239 Size: 393 539 426 783 597 787 268 1524 Fragments arranged by size: 1524 787 783 597 539 426 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2348 2348 Size: 5317 EcoO109I rG'GnC_Cy Cuts at: 2105 2296 2365 2393 2545 2105 Size: 191 69 28 152 4877 Fragments arranged by size: 4877 191 152 69 28 EcoRI G'AATT_C Cuts at: 2626 3004 2626 Size: 378 4939 FspI TGC'GCA Cuts at: 3823 3823 Size: 5317 HaeII r_GCGC'y Cuts at: 1678 2186 2533 4464 1678 Size: 508 347 1931 2531 Fragments arranged by size: 2531 1931 508 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3958 3990 4032 4064 1373 Size: 32 135 32 2386 32 42 32 2626 Fragments arranged by size: 2626 2386 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2661 2907 669 Size: 988 281 723 246 3079 Fragments arranged by size: 3079 988 723 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5317 HpaI GTT'AAC Cuts at: 2907 2907 Size: 5317 KpnI G_GTAC'C Cuts at: 2642 2642 Size: 5317 MfeI C'AATT_G Cuts at: 2916 2916 Size: 5317 MluI A'CGCG_T Cuts at: 5193 5193 Size: 5317 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4431 4615 1305 Size: 3126 184 2007 Fragments arranged by size: 3126 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3495 Fragments arranged by size: 3495 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3275 3634 3793 518 Size: 648 2109 359 159 2042 Fragments arranged by size: 2109 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2053 2283 2305 2416 2733 3410 4558 4803 Size: 447 230 22 111 317 677 1148 245 Cuts at: 4803 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 447 317 245 230 111 22 NaeI GCC'GGC Cuts at: 2508 2508 Size: 5317 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3826 Fragments arranged by size: 3826 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5317 NgoMIV G'CCGG_C Cuts at: 2506 2506 Size: 5317 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5317 NotI GC'GGCC_GC Cuts at: 2764 2764 Size: 5317 NspI r_CATG'y Cuts at: 1132 1229 2675 4220 1132 Size: 97 1446 1545 2229 Fragments arranged by size: 2229 1545 1446 97 PasI CC'CwG_GG Cuts at: 1348 2101 2265 2494 1348 Size: 753 164 229 4171 Fragments arranged by size: 4171 753 229 164 PciI A'CATG_T Cuts at: 4216 4216 Size: 5317 PflMI CCAn_nnn'nTGG Cuts at: 1108 2445 1108 Size: 1337 3980 PfoI T'CCnGG_A Cuts at: 2187 2574 2187 Size: 387 4930 PmeI GTTT'AAAC Cuts at: 2621 2621 Size: 5317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3386 3418 4899 4931 3386 Size: 32 1481 32 3772 Fragments arranged by size: 3772 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2393 2545 2393 Size: 152 5165 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5317 PsiI TTA'TAA Cuts at: 2887 2887 Size: 5317 PspOMI G'GGCC_C Cuts at: 2104 2104 Size: 5317 PstI C_TGCA'G Cuts at: 830 2052 2304 2364 2415 2487 2568 2669 830 Size: 1222 252 60 51 72 81 101 3478 Fragments arranged by size: 3478 1222 252 101 81 72 60 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5317 PvuII CAG'CTG Cuts at: 2053 2283 2305 2416 2053 Size: 230 22 111 4954 Fragments arranged by size: 4954 230 111 22 SalI G'TCGA_C Cuts at: 1936 2659 1936 Size: 723 4594 SanDI GG'GwC_CC Cuts at: 2393 2393 Size: 5317 SbfI CC_TGCA'GG Cuts at: 2364 2669 2364 Size: 305 5012 ScaI AGT'ACT Cuts at: 3565 5277 3565 Size: 1712 3605 SfcI C'TryA_G Cuts at: 826 1045 2048 2300 2360 2411 2483 2564 2665 Size: 219 1003 252 60 51 72 81 101 Cuts at: 2665 3800 4481 4672 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 681 252 219 191 101 81 72 60 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5317 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5317 SmaI CCC'GGG Cuts at: 1872 2644 1872 Size: 772 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2138 2317 3421 4322 4584 820 Size: 197 926 195 179 1104 901 262 1553 Fragments arranged by size: 1553 1104 926 901 262 197 195 179 SnaBI TAC'GTA Cuts at: 493 3108 493 Size: 2615 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5317 SphI G_CATG'C Cuts at: 1132 2675 1132 Size: 1543 3774 SspI AAT'ATT Cuts at: 5 52 3241 5 Size: 47 3189 2081 Fragments arranged by size: 3189 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5317 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2756 513 Size: 552 939 396 356 3074 Fragments arranged by size: 3074 939 552 396 356 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 2507 3344 3361 3514 3699 4144 Size: 1255 456 471 837 17 153 185 445 Cuts at: 4144 325 Size: 1498 Fragments arranged by size: 1498 1255 837 471 456 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3563 5275 96 Size: 274 80 33 51 1443 1586 1712 138 Fragments arranged by size: 1712 1586 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2183 2215 5277 5309 2183 Size: 32 3062 32 2191 Fragments arranged by size: 3062 2191 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5317 XbaI T'CTAG_A Cuts at: 2653 2653 Size: 5317 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2038 Size: 5317 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5317 XmaI C'CCGG_G Cuts at: 1870 2642 1870 Size: 772 4545 XmnI GAAnn'nnTTC Cuts at: 3446 3446 Size: 5317 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2422 3125 276 Size: 53 83 186 936 888 703 2468 Fragments arranged by size: 2468 936 888 703 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI