(Linear) (Six-base) MAPSORT of: KIEE1702.seq Check: 6224 from: 1 to: 612 KIEE1702.orf With 182 enzymes: SgfI * July 15, 2010 18:45 .. AatII G_ACGT'C Cuts at: 0 419 612 Size: 419 193 AfeI AGC'GCT Cuts at: 0 179 612 Size: 179 433 AlwNI CAG_nnn'CTG Cuts at: 0 51 294 363 612 Size: 51 243 69 249 Fragments arranged by size: 249 243 69 51 ApaI G_GGCC'C Cuts at: 0 103 612 Size: 103 509 ApaLI G'TGCA_C Cuts at: 0 65 612 Size: 65 547 AvaI C'yCGr_G Cuts at: 0 220 379 612 Size: 220 159 233 Fragments arranged by size: 233 220 159 BanI G'GyrC_C Cuts at: 0 493 612 Size: 493 119 BanII G_rGCy'C Cuts at: 0 25 103 267 353 612 Size: 25 78 164 86 259 Fragments arranged by size: 259 164 86 78 25 BglI GCCn_nnn'nGGC Cuts at: 0 502 612 Size: 502 110 Bme1580I G_kGCm'C Cuts at: 0 69 103 612 Size: 69 34 509 Fragments arranged by size: 509 69 34 BmrI ACTGGGnnnn_n' Cuts at: 0 469 612 Size: 469 143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 592 612 Size: 166 426 20 Fragments arranged by size: 426 166 20 Bpu10I CC'TnA_GC Cuts at: 0 271 612 Size: 271 341 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 333 612 Size: 118 215 279 Fragments arranged by size: 279 215 118 BsaHI Gr'CG_yC Cuts at: 0 416 612 Size: 416 196 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 178 208 565 595 612 Size: 178 30 357 30 17 Fragments arranged by size: 357 178 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 238 612 Size: 238 374 BseYI C'CCAG_C Cuts at: 0 283 605 612 Size: 283 322 7 Fragments arranged by size: 322 283 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 612 Size: 100 512 BsiHKAI G_wGCw'C Cuts at: 0 69 612 Size: 69 543 Bsp1286I G_dGCh'C Cuts at: 0 25 69 103 267 353 612 Size: 25 44 34 164 86 259 Fragments arranged by size: 259 164 86 44 34 25 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 18 612 Size: 18 594 BsrFI r'CCGG_y Cuts at: 0 501 612 Size: 501 111 BstAPI GCAn_nnn'nTGC Cuts at: 0 51 612 Size: 51 561 BstXI CCAn_nnnn'nTGG Cuts at: 0 96 169 612 Size: 96 73 443 Fragments arranged by size: 443 96 73 BstYI r'GATC_y Cuts at: 0 158 371 612 Size: 158 213 241 Fragments arranged by size: 241 213 158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 182 612 Size: 182 430 BtsI GCAGTG_nn' Cuts at: 0 403 612 Size: 403 209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 592 612 Size: 166 426 20 Fragments arranged by size: 426 166 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 343 612 Size: 343 269 EcoO109I rG'GnC_Cy Cuts at: 0 100 291 360 388 540 612 Size: 100 191 69 28 152 72 Fragments arranged by size: 191 152 100 72 69 28 HaeII r_GCGC'y Cuts at: 0 181 528 612 Size: 181 347 84 Fragments arranged by size: 347 181 84 MspA1I CmG'CkG Cuts at: 0 48 278 300 411 612 Size: 48 230 22 111 201 Fragments arranged by size: 230 201 111 48 22 NaeI GCC'GGC Cuts at: 0 503 612 Size: 503 109 NgoMIV G'CCGG_C Cuts at: 0 501 612 Size: 501 111 PasI CC'CwG_GG Cuts at: 0 96 260 489 612 Size: 96 164 229 123 Fragments arranged by size: 229 164 123 96 PflMI CCAn_nnn'nTGG Cuts at: 0 440 612 Size: 440 172 PfoI T'CCnGG_A Cuts at: 0 182 569 612 Size: 182 387 43 Fragments arranged by size: 387 182 43 PpuMI rG'GwC_Cy Cuts at: 0 388 540 612 Size: 388 152 72 Fragments arranged by size: 388 152 72 PspOMI G'GGCC_C Cuts at: 0 99 612 Size: 99 513 PstI C_TGCA'G Cuts at: 0 47 299 359 410 482 563 612 Size: 47 252 60 51 72 81 49 Fragments arranged by size: 252 81 72 60 51 49 47 PvuII CAG'CTG Cuts at: 0 48 278 300 411 612 Size: 48 230 22 111 201 Fragments arranged by size: 230 201 111 48 22 SanDI GG'GwC_CC Cuts at: 0 388 612 Size: 388 224 SbfI CC_TGCA'GG Cuts at: 0 359 612 Size: 359 253 SfcI C'TryA_G Cuts at: 0 43 295 355 406 478 559 612 Size: 43 252 60 51 72 81 53 Fragments arranged by size: 252 81 72 60 53 51 43 SmlI C'TyrA_G Cuts at: 0 133 312 612 Size: 133 179 300 Fragments arranged by size: 300 179 133 StyI C'CwwG_G Cuts at: 0 395 612 Size: 395 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 31 502 612 Size: 31 471 110 Fragments arranged by size: 471 110 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 178 210 612 Size: 178 32 402 Fragments arranged by size: 402 178 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 33 612 Size: 33 579 ZraI GAC'GTC Cuts at: 0 417 612 Size: 417 195 Enzymes that do cut: AatII AfeI AlwNI ApaI ApaLI AvaI BanI BanII BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI BtgZI BtsI Eco57MI EcoNI EcoO109I HaeII MspA1I NaeI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SbfI SfcI SmlI StyI TaqII TstI XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI