(Linear) (Six-base) MAPSORT of: KIEE4113.seq Check: 8660 from: 1 to: 1455 KIEE4113.orf With 182 enzymes: SgfI * March 22, 2012 17:23 .. Acc65I G'GTAC_C Cuts at: 0 1055 1455 Size: 1055 400 AfeI AGC'GCT Cuts at: 0 616 1455 Size: 616 839 AflIII A'CryG_T Cuts at: 0 428 1455 Size: 428 1027 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1247 1279 1455 Size: 1247 32 176 Fragments arranged by size: 1247 176 32 AlwNI CAG_nnn'CTG Cuts at: 0 693 792 1455 Size: 693 99 663 Fragments arranged by size: 693 663 99 ApaI G_GGCC'C Cuts at: 0 272 1455 Size: 272 1183 ApaLI G'TGCA_C Cuts at: 0 253 1455 Size: 253 1202 ApoI r'AATT_y Cuts at: 0 377 851 1455 Size: 377 474 604 Fragments arranged by size: 604 474 377 AvaI C'yCGr_G Cuts at: 0 273 601 710 1180 1455 Size: 273 328 109 470 275 Fragments arranged by size: 470 328 275 273 109 BamHI G'GATC_C Cuts at: 0 1225 1455 Size: 1225 230 BanI G'GyrC_C Cuts at: 0 64 291 1010 1055 1455 Size: 64 227 719 45 400 Fragments arranged by size: 719 400 227 64 45 BanII G_rGCy'C Cuts at: 0 178 242 272 401 602 837 1172 1442 Size: 178 64 30 129 201 235 335 270 Cuts at: 1442 1455 Size: 13 Fragments arranged by size: 335 270 235 201 178 129 64 30 13 BbeI G_GCGC'C Cuts at: 0 68 1455 Size: 68 1387 BbsI GAAGACnn'nnnn_ Cuts at: 0 324 735 1019 1455 Size: 324 411 284 436 Fragments arranged by size: 436 411 324 284 BglI GCCn_nnn'nGGC Cuts at: 0 649 1127 1455 Size: 649 478 328 Fragments arranged by size: 649 478 328 Bme1580I G_kGCm'C Cuts at: 0 257 272 296 1455 Size: 257 15 24 1159 Fragments arranged by size: 1159 257 24 15 BmgBI CAC'GTC Cuts at: 0 252 393 1455 Size: 252 141 1062 Fragments arranged by size: 1062 252 141 BmrI ACTGGGnnnn_n' Cuts at: 0 771 888 1421 1455 Size: 771 117 533 34 Fragments arranged by size: 771 533 117 34 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 715 934 1116 1455 Size: 556 159 219 182 339 Fragments arranged by size: 556 339 219 182 159 Bpu10I CC'TnA_GC Cuts at: 0 1436 1455 Size: 1436 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1156 1455 Size: 1156 299 BsaI GGTCTCn'nnnn_ Cuts at: 0 347 913 1455 Size: 347 566 542 Fragments arranged by size: 566 542 347 BsaHI Gr'CG_yC Cuts at: 0 65 1455 Size: 65 1390 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 610 886 931 1380 1455 Size: 610 276 45 449 75 Fragments arranged by size: 610 449 276 75 45 BseYI C'CCAG_C Cuts at: 0 103 504 620 1116 1455 Size: 103 401 116 496 339 Fragments arranged by size: 496 401 339 116 103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1375 1455 Size: 1375 80 BsiEI CG_ry'CG Cuts at: 0 38 1455 Size: 38 1417 BsiHKAI G_wGCw'C Cuts at: 0 242 257 1172 1390 1442 1455 Size: 242 15 915 218 52 13 Fragments arranged by size: 915 242 218 52 15 13 Bsp1286I G_dGCh'C Cuts at: 0 178 242 257 272 296 401 602 837 Size: 178 64 15 15 24 105 201 235 Cuts at: 837 1172 1390 1442 1455 Size: 335 218 52 13 Fragments arranged by size: 335 235 218 201 178 105 64 52 24 15 15 13 BsrFI r'CCGG_y Cuts at: 0 61 584 683 1455 Size: 61 523 99 772 Fragments arranged by size: 772 523 99 61 BsrGI T'GTAC_A Cuts at: 0 431 1455 Size: 431 1024 BstAPI GCAn_nnn'nTGC Cuts at: 0 792 1455 Size: 792 663 BstYI r'GATC_y Cuts at: 0 1225 1430 1455 Size: 1225 205 25 Fragments arranged by size: 1225 205 25 BtgI C'CryG_G Cuts at: 0 32 38 71 1059 1455 Size: 32 6 33 988 396 Fragments arranged by size: 988 396 33 32 6 BtsI GCAGTG_nn' Cuts at: 0 1094 1363 1455 Size: 1094 269 92 Fragments arranged by size: 1094 269 92 ClaI AT'CG_AT Cuts at: 0 446 1455 Size: 446 1009 EaeI y'GGCC_r Cuts at: 0 35 245 470 947 1292 1455 Size: 35 210 225 477 345 163 Fragments arranged by size: 477 345 225 210 163 35 EagI C'GGCC_G Cuts at: 0 35 1455 Size: 35 1420 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 22 376 787 1455 Size: 22 354 411 668 Fragments arranged by size: 668 411 354 22 EcoICRI GAG'CTC Cuts at: 0 240 1170 1440 1455 Size: 240 930 270 15 Fragments arranged by size: 930 270 240 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 715 934 1116 1455 Size: 556 159 219 182 339 Fragments arranged by size: 556 339 219 182 159 EcoO109I rG'GnC_Cy Cuts at: 0 268 269 717 993 1455 Size: 268 1 448 276 462 Fragments arranged by size: 462 448 276 268 1 EcoRI G'AATT_C Cuts at: 0 851 1455 Size: 851 604 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 327 1389 1455 Size: 327 1062 66 Fragments arranged by size: 1062 327 66 HaeII r_GCGC'y Cuts at: 0 32 68 110 124 618 890 1068 1455 Size: 32 36 42 14 494 272 178 387 Fragments arranged by size: 494 387 272 178 42 36 32 14 HincII GTy'rAC Cuts at: 0 528 1455 Size: 528 927 KasI G'GCGC_C Cuts at: 0 64 1455 Size: 64 1391 KpnI G_GTAC'C Cuts at: 0 1059 1455 Size: 1059 396 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 880 1455 Size: 880 575 MscI TGG'CCA Cuts at: 0 472 1455 Size: 472 983 MspA1I CmG'CkG Cuts at: 0 34 40 1061 1455 Size: 34 6 1021 394 Fragments arranged by size: 1021 394 34 6 NaeI GCC'GGC Cuts at: 0 63 586 1455 Size: 63 523 869 Fragments arranged by size: 869 523 63 NarI GG'CG_CC Cuts at: 0 65 1455 Size: 65 1390 NgoMIV G'CCGG_C Cuts at: 0 61 584 1455 Size: 61 523 871 Fragments arranged by size: 871 523 61 NotI GC'GGCC_GC Cuts at: 0 35 1455 Size: 35 1420 NspI r_CATG'y Cuts at: 0 432 823 1455 Size: 432 391 632 Fragments arranged by size: 632 432 391 PciI A'CATG_T Cuts at: 0 428 1455 Size: 428 1027 PfoI T'CCnGG_A Cuts at: 0 1304 1455 Size: 1304 151 PshAI GACnn'nnGTC Cuts at: 0 1414 1455 Size: 1414 41 PspOMI G'GGCC_C Cuts at: 0 268 1455 Size: 268 1187 RsrII CG'GwC_CG Cuts at: 0 659 1455 Size: 659 796 SacI G_AGCT'C Cuts at: 0 242 1172 1442 1455 Size: 242 930 270 13 Fragments arranged by size: 930 270 242 13 SacII CC_GC'GG Cuts at: 0 35 41 1062 1455 Size: 35 6 1021 393 Fragments arranged by size: 1021 393 35 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 649 1455 Size: 649 806 SfoI GGC'GCC Cuts at: 0 66 1455 Size: 66 1389 SgrAI Cr'CCGG_yG Cuts at: 0 61 1455 Size: 61 1394 SmaI CCC'GGG Cuts at: 0 275 1455 Size: 275 1180 SmlI C'TyrA_G Cuts at: 0 1171 1455 Size: 1171 284 TatI w'GTAC_w Cuts at: 0 425 431 494 1151 1455 Size: 425 6 63 657 304 Fragments arranged by size: 657 425 304 63 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1342 1374 1455 Size: 1342 32 81 Fragments arranged by size: 1342 81 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 735 1302 1455 Size: 735 567 153 Fragments arranged by size: 735 567 153 XmaI C'CCGG_G Cuts at: 0 273 1455 Size: 273 1182 Enzymes that do cut: Acc65I AfeI AflIII AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BsrGI BstAPI BstYI BtgI BtsI ClaI EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII HincII KasI KpnI MmeI MscI MspA1I NaeI NarI NgoMIV NotI NspI PciI PfoI PshAI PspOMI RsrII SacI SacII SfiI SfoI SgrAI SmaI SmlI TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AflII AgeI AhdI AleI AlfI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EarI EcoNI EcoRV FseI FspI FspAI Hin4I HindIII HpaI MfeI MluI MslI NcoI NdeI NheI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI