################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AccIII AclI AcsI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvrII BbeI BciVI BglII BlnI BsaBI BseAI BsiWI BsmBI Bsp1407I BspEI BspLU11I BsrGI Bst1107I BstBI BstZ17I Bsu36I CpoI DraI DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FseI HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII ScaI SexAI SfoI SfuI SnaBI SpeI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 682 GACGTC GACGT^C AflII 2026 CTTAAG C^TTAAG AflIII 2723 ACACGT A^C[GA][CT]GT AgeI 2320 ACCGGT A^CCGGT Aor51HI 36 AGCGCT AGC^GCT BanI 3478 GGTGCC G^G[CT][GA]CC BbsI 2961 GAAGAC GAAGAC(2/6) BcgI 2149 CGAATCGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 599 TGATCA T^GATCA BfaI 1090 CTAG C^TAG BfrI 2026 CTTAAG C^TTAAG BpuAI 2961 GAAGAC GAAGAC(2/6) BsiEI 2180 CGGCCG CG[GA][CT]^CG BspDI 2917 ATCGAT AT^CGAT BsrDI 2351 CATTGC GCAATG(2/0) ClaI 2917 ATCGAT AT^CGAT EagI 2180 CGGCCG C^GGCCG EclXI 2180 CGGCCG C^GGCCG Eco47III 36 AGCGCT AGC^GCT Eco52I 2180 CGGCCG C^GGCCG FbaI 599 TGATCA T^GATCA HindIII 2536 AAGCTT A^AGCTT MaeI 1090 CTAG C^TAG NotI 2179 GCGGCCGC GC^GGCCGC NspI 2159 GCATGC [GA]CATG^[CT] PinAI 2320 ACCGGT A^CCGGT SacI 550 GAGCTC GAGCT^C SalI 796 GTCGAC G^TCGAC SapI 1908 GAAGAGC GCTCTTC(1/4) SbfI 3055 CCTGCAGG CCTGCA^GG SfiI 1857 GGCCCCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 2319 CACCGGTG C[GA]^CCGG[CT]G SphI 2159 GCATGC GCATG^C Sse8387I 3055 CCTGCAGG CCTGCA^GG StuI 1392 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 796 GTCGAC GT^[AC][GT]AC 3148 GTAGAC AspI 2370 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3460 GACGCTGTC AviII 158 TGCGCA TGC^GCA 1685 TGCGCA BbvCI 354 CCTCAGC CCTCAGC(-5/-2) 3169 CCTCAGC BlpI 591 GCTTAGC GC^T[ACGT]AGC 1640 GCTCAGC Bpu1102I 591 GCTTAGC GC^T[ACGT]AGC 1640 GCTCAGC BsaWI 2320 ACCGGT [AT]^CCGG[AT] 2786 ACCGGA BsmI 991 GAATGC GAATGC(1/-1) 2068 GCATTC BspHI 323 TCATGA T^CATGA 542 TCATGA BsrFI 2320 ACCGGT [GA]^CCGG[CT] 3303 GCCGGT BssSI 2047 CACGAG CACGAG(-5/-1) 952 CTCGTG BstEII 582 GGTCACC G^GT[ACGT]ACC 3591 GGTGACC BstPI 582 GGTCACC G^GT[ACGT]ACC 3591 GGTGACC CelII 591 GCTTAGC GC^T[ACGT]AGC 1640 GCTCAGC Cfr10I 2320 ACCGGT [GA]^CCGG[CT] 3303 GCCGGT DraIII 838 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2404 CACCACGTG EarI 1908 GAAGAG CTCTTC(1/4) 3691 GAAGAG EcoO65I 582 GGTCACC G^GT[ACGT]ACC 3591 GGTGACC FspI 158 TGCGCA TGC^GCA 1685 TGCGCA HincII 142 GTCAAC GT[CT]^[GA]AC 796 GTCGAC HindII 142 GTCAAC GT[CT]^[GA]AC 796 GTCGAC Ksp632I 1908 GAAGAG CTCTTC(1/4) 3691 GAAGAG KspI 258 CCGCGG CCGC^GG 2976 CCGCGG MseI 1943 TTAA T^TAA 2027 TTAA PflFI 2370 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3460 GACGCTGTC RcaI 323 TCATGA T^CATGA 542 TCATGA SacII 258 CCGCGG CCGC^GG 2976 CCGCGG SmlI 2026 CTTAAG C^T[CT][GA]AG 3340 CTCAAG Tru9I 1943 TTAA T^TAA 2027 TTAA Tsp509I 1940 AATT ^AATT 2289 AATT Tth111I 2370 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3460 GACGCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI Asp700I AspHI AvaI AvaII BalI BamHI BanII BbrPI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI