################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 364 GCGG CCGC(-3/-1) AclI 376 AACGTT AA^CGTT AluI 317 AGCT AG^CT AlwI 417 GGATC GGATC(4/5) AlwNI 141 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 329 GGTCC G^G[AT]CC BbvI 224 GCAGC GCAGC(8/12) BcnI 132 CCGGG CC^[GC]GG BpmI 81 CTCCAG CTGGAG(16/14) BseRI 162 GAGGAG GAGGAG(10/8) BsmAI 165 GAGAC GTCTC(1/5) BsmBI 165 GAGACG CGTCTC(1/5) BsmFI 330 GTCCC GGGAC(10/14) BspHI 158 TCATGA T^CATGA BsrI 348 CCAGT ACTGG(1/-1) BstEII 303 GGTCACC G^GT[ACGT]ACC BstF5I 135 GGATG GGATG(2/0) BstPI 303 GGTCACC G^GT[ACGT]ACC Cac8I 314 GCAAGC GC[ACGT]^[ACGT]GC CfoI 222 GCGC GCG^C DpnII 418 GATC ^GATC EcoNI 66 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 303 GGTCACC G^GT[ACGT]ACC FauI 364 GCGGG CCCGC(4/6) Fnu4HI 224 GCAGC GC^[ACGT]GC FokI 135 GGATG GGATG(9/13) HapII 132 CCGG C^CGG HhaI 222 GCGC GCG^C HinP1I 222 GCGC G^CGC HpaII 132 CCGG C^CGG ItaI 224 GCAGC GC^[ACGT]GC MaeII 377 ACGT A^CGT MaeIII 304 GTCAC ^GT[ACGT]AC MboI 418 GATC ^GATC MslI 109 CACTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 362 CAGCGG C[AC]G^C[GT]G MspI 132 CCGG C^CGG MwoI 215 GCAACAAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 132 CCGGG CC^[GC]GG NdeII 418 GATC ^GATC PpuMI 328 GGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 376 AACGTT AA^CGTT RcaI 158 TCATGA T^CATGA Sau3AI 418 GATC ^GATC TseI 224 GCAGC G^C[AT]GC Tsp45I 304 GTCAC ^GT[GC]AC Tsp509I 426 AATT ^AATT ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AfaI 87 GTAC GT^AC 351 GTAC BbsI 31 GTCTTC GAAGAC(2/6) 291 GTCTTC BpuAI 31 GTCTTC GAAGAC(2/6) 291 GTCTTC BsiYI 66 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 359 CCACAGCGGGG BslI 66 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 359 CCACAGCGGGG DdeI 71 CTCAG C^T[ACGT]AG 277 CTAAG DraII 170 GGGGCCT [GA]G^G[ACGT]CC[CT] 328 GGGTCCC EcoO109I 170 GGGGCCT [GA]G^G[ACGT]CC[CT] 328 GGGTCCC MboII 32 TCTTC GAAGA(8/7) 292 TCTTC RsaI 87 GTAC GT^AC 351 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BsaJI BssKI BstNI Cfr13I EcoRII HaeIII HphI MnlI MvaI NlaIII NlaIV Sau96I ScrFI SfaNI