################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcsI AcyI AflII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspHI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 269 GGTACC G^GTACC AflIII 257 ACATGT A^C[GA][CT]GT AhdI 695 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 393 GATCC GGATC(4/5) ApaI 445 GGGCCC GGGCC^C Asp700I 43 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 269 GGTACC G^GTACC AspEI 695 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 836 TGGCCA TGG^CCA BciVI 10 GGATAC GTATCC(6/5) BglII 137 AGATCT A^GATCT BpmI 754 CTGGAG CTGGAG(16/14) BsaJI 329 CCCTGG C^C[ACGT][ACGT]GG BsaWI 500 ACCGGA [AT]^CCGG[AT] BsgI 800 CTGCAC GTGCAG(16/14) BsiEI 498 CGACCG CG[GA][CT]^CG BsmI 842 GCATTC GAATGC(1/-1) BspLU11I 257 ACATGT A^CATGT BspMI 248 GCAGGT ACCTGC(4/8) BstXI 827 CCATTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 137 AGATCT [GA]^GATC[CT] Eam1105I 695 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 382 GATATC GAT^ATC HapII 501 CCGG C^CGG HgaI 643 GACGC GACGC(5/10) HinfI 701 GAGTC G^A[ACGT]TC HpaII 501 CCGG C^CGG KpnI 269 GGTACC GGTAC^C MaeII 552 ACGT A^CGT MflI 137 AGATCT [GA]^GATC[CT] MluNI 836 TGGCCA TGG^CCA MscI 836 TGGCCA TGG^CCA MseI 124 TTAA T^TAA MspA1I 785 CAGCTG C[AC]G^C[GT]G MspI 501 CCGG C^CGG NspI 257 ACATGT [GA]CATG^[CT] PflMI 309 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 701 GAGTC GAGTC(4/5) PvuII 785 CAGCTG CAG^CTG SexAI 414 ACCTGGT A^CC[AT]GGT SmlI 74 CTCAAG C^T[CT][GA]AG TaqI 699 TCGA T^CGA Tru9I 124 TTAA T^TAA TthHB8I 699 TCGA T^CGA Van91I 309 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 137 AGATCT [GA]^GATC[CT] XmnI 43 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BanI 269 GGTACC G^G[CT][GA]CC 568 GGCACC BanII 445 GGGCCC G[GA]GC[CT]^C 518 GGGCTC BmyI 445 GGGCCC G[AGT]GC[ACT]^C 518 GGGCTC Bsp1286I 445 GGGCCC G[AGT]GC[ACT]^C 518 GGGCTC EaeI 195 TGGCCG [CT]^GGCC[GA] 836 TGGCCA EcoNI 436 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 753 CCTGGAGAAGG MboII 22 GAAGA GAAGA(8/7) 772 GAAGA MwoI 549 GCAACGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 624 GCTGCAGAAGC PstI 38 CTGCAG CTGCA^G 625 CTGCAG SfaNI 240 GATGC GCATC(5/9) 490 GATGC Tsp509I 122 AATT ^AATT 809 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AfaI AluI AlwNI BbvI BsiYI BslI BsmAI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI MaeIII MboI MnlI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I TspRI