(Circular) (Six-base) MAPSORT of: pFN21AE1781.seq Check: 8986 from: 1 to: 5572 pFN21AE1781 5572 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 1937 Size: 978 4594 Acc65I G'GTAC_C Cuts at: 2274 2893 2274 Size: 619 4953 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3635 5019 5287 1632 Size: 2003 1384 268 1917 Fragments arranged by size: 2003 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 2262 4471 5448 1203 Size: 1059 2209 977 1327 Fragments arranged by size: 2209 1327 1059 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 2705 4301 2705 Size: 1596 3976 AleI CACnn'nnGTG Cuts at: 1166 2579 1166 Size: 1413 4159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AlwNI CAG_nnn'CTG Cuts at: 2235 2535 2638 2651 3315 4887 2235 Size: 300 103 13 664 1572 2920 Fragments arranged by size: 2920 1572 664 300 103 13 ApaI G_GGCC'C Cuts at: 2454 2454 Size: 5572 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2897 1870 Size: 73 954 4545 Fragments arranged by size: 4545 954 73 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2274 2573 2893 4348 618 Size: 325 217 627 487 299 320 1455 1842 Fragments arranged by size: 1842 1455 627 487 325 320 299 217 BanII G_rGCy'C Cuts at: 1391 2454 2527 2891 1391 Size: 1063 73 364 4072 Fragments arranged by size: 4072 1063 364 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4601 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2009 3464 4674 2009 Size: 1455 1210 2907 Fragments arranged by size: 2907 1455 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4183 136 Size: 107 122 71 631 605 2511 1525 Fragments arranged by size: 2511 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2142 5567 2142 Size: 3425 2147 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 2454 3633 4789 1790 Size: 664 1179 1156 2573 Fragments arranged by size: 2573 1179 1156 664 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2294 3303 4261 Size: 416 53 83 399 24 877 1009 958 Cuts at: 4261 442 Size: 1753 Fragments arranged by size: 1753 1009 958 877 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2780 4232 1239 Size: 1541 1452 2579 Fragments arranged by size: 2579 1541 1452 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2064 3697 4562 4860 2064 Size: 1633 865 298 2776 Fragments arranged by size: 2776 1633 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4235 882 Size: 617 2736 2219 Fragments arranged by size: 2736 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3363 493 Size: 711 2159 2702 Fragments arranged by size: 2702 2159 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3379 3761 4396 275 Size: 53 83 186 936 1846 382 635 1451 Fragments arranged by size: 1846 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 4005 4403 4677 4824 5235 2505 Size: 1500 398 274 147 411 2842 Fragments arranged by size: 2842 1500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5347 Fragments arranged by size: 5347 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5572 BseYI C'CCAG_C Cuts at: 2597 2638 4775 5271 2597 Size: 41 2137 496 2898 Fragments arranged by size: 2898 2137 496 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2790 1163 Size: 573 1054 3945 Fragments arranged by size: 3945 1054 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2506 3022 3783 4811 1540 Size: 275 186 505 516 761 1028 2301 Fragments arranged by size: 2301 1028 761 516 505 275 186 BsiHKAI G_wGCw'C Cuts at: 3633 3718 4789 3633 Size: 85 1071 4416 Fragments arranged by size: 4416 1071 85 BsmI GAATG_Cn' Cuts at: 2847 3083 3176 2847 Size: 236 93 5243 Fragments arranged by size: 5243 236 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2454 2527 2891 3633 3718 4789 1391 Size: 399 664 73 364 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 399 364 85 73 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2927 844 Size: 1400 683 3489 Fragments arranged by size: 3489 1400 683 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4067 4241 65 Size: 1183 2819 174 1396 Fragments arranged by size: 2819 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4216 4403 1700 Size: 110 2406 187 2869 Fragments arranged by size: 2869 2406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5572 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstXI CCAn_nnnn'nTGG Cuts at: 2839 2839 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2142 2902 3269 3654 3671 5123 5209 5221 Size: 170 760 367 385 17 1452 86 12 Cuts at: 5221 5567 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 760 385 367 346 170 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4081 Fragments arranged by size: 4081 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4833 Fragments arranged by size: 4833 683 56 BtsI GCAGTG_nn' Cuts at: 2040 3088 3882 3902 2040 Size: 1048 794 20 3710 Fragments arranged by size: 3710 1048 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2200 2841 3019 Size: 54 1425 50 275 18 370 641 178 Cuts at: 3019 3908 5537 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 641 370 275 178 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3019 1537 Size: 275 1207 4090 Fragments arranged by size: 4090 1207 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3502 1353 Size: 2149 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2780 3635 4232 5019 5287 1239 Size: 393 1148 855 597 787 268 1524 Fragments arranged by size: 1524 1148 855 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2445 2762 2445 Size: 317 5255 EcoO109I rG'GnC_Cy Cuts at: 2232 2450 2594 2232 Size: 218 144 5210 Fragments arranged by size: 5210 218 144 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 EcoRV GAT'ATC Cuts at: 2389 2389 Size: 5572 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 4719 1678 Size: 3041 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2691 2723 4213 4245 4287 Size: 32 135 32 1119 32 1490 32 42 Cuts at: 4287 4319 1373 Size: 32 2626 Fragments arranged by size: 2626 1490 1119 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3162 669 Size: 988 281 978 246 3079 Fragments arranged by size: 3079 988 978 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2278 2897 2278 Size: 619 4953 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2362 4686 4870 1305 Size: 1057 2324 184 2007 Fragments arranged by size: 2324 2007 1057 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2843 10 Size: 54 1425 343 1011 2739 Fragments arranged by size: 2739 1425 1011 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2265 2579 2837 3530 3889 4048 518 Size: 648 1099 314 258 693 359 159 2042 Fragments arranged by size: 2042 1099 693 648 359 314 258 159 MspA1I CmG'CkG Cuts at: 1606 2792 2988 3665 4813 5058 1606 Size: 1186 196 677 1148 245 2120 Fragments arranged by size: 2120 1186 1148 677 245 196 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4081 Fragments arranged by size: 4081 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2266 2930 4475 1132 Size: 97 1037 664 1545 2229 Fragments arranged by size: 2229 1545 1037 664 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5572 PciI A'CATG_T Cuts at: 2262 4471 2262 Size: 2209 3363 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 1108 Size: 1212 4360 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 2034 3142 2034 Size: 1108 4464 PspOMI G'GGCC_C Cuts at: 2450 2450 Size: 5572 PstI C_TGCA'G Cuts at: 830 2047 2634 2924 830 Size: 1217 587 290 3478 Fragments arranged by size: 3478 1217 587 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 PvuII CAG'CTG Cuts at: 2792 2792 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SexAI A'CCwGG_T Cuts at: 2419 2419 Size: 5572 SfcI C'TryA_G Cuts at: 826 1045 2043 2564 2630 2920 4055 4736 4927 Size: 219 998 521 66 290 1135 681 191 Cuts at: 4927 826 Size: 1471 Fragments arranged by size: 1471 1135 998 681 521 290 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2079 3676 4577 4839 820 Size: 197 926 136 1597 901 262 1553 Fragments arranged by size: 1597 1553 926 901 262 197 136 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 2004 3011 513 Size: 552 939 1007 3074 Fragments arranged by size: 3074 1007 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2312 3599 3616 3769 3954 4399 325 Size: 1255 732 1287 17 153 185 445 1498 Fragments arranged by size: 1498 1287 1255 732 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2180 2663 3818 Size: 274 80 33 51 1443 203 483 1155 Cuts at: 3818 5530 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1155 483 274 203 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5532 5564 5532 Size: 32 5540 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5572 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 2052 3701 2052 Size: 1649 3923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI